{"title":"Gap Junction Protein Connexin-43 in a Rat Dorsal Root Ganglion","authors":"E. A. Kolos, D. E. Korzhevsky","doi":"10.1134/s1990519x23700049","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The study aimed at investigating the dynamics of the distribution and localization of the gap junction protein connexin-43 (Cx43) in rat dorsal root ganglion (DRG) cells at different stages of postnatal ontogenesis to assess morphological signs of age-related changes in intercellular interactions. The work was performed on Wistar rats aged 4 and 18 months using immunohistochemical methods. Glial cells were detected using antibodies to glutamine synthetase, and Iba-1 marker was used for macrophages. It has been established that connexin-43-containing structures are identified predominantly in satellite glial cells of young and aging animals. Sensory neurons, as well as DRG macrophages, of the examined age groups do not contain connexin-43. When analyzing age-related changes in intercellular contacts in the DRG of rats, it was found that areas enriched with connexin-43, corresponding to plaques of protein channels that ensure metabolic interaction of satellite glial cells in the dorsal root ganglia, became more numerous with age. This fact may indicate activation of interaction between glial cells in the sensory ganglia during rat aging.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"207 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell and Tissue Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1134/s1990519x23700049","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
The study aimed at investigating the dynamics of the distribution and localization of the gap junction protein connexin-43 (Cx43) in rat dorsal root ganglion (DRG) cells at different stages of postnatal ontogenesis to assess morphological signs of age-related changes in intercellular interactions. The work was performed on Wistar rats aged 4 and 18 months using immunohistochemical methods. Glial cells were detected using antibodies to glutamine synthetase, and Iba-1 marker was used for macrophages. It has been established that connexin-43-containing structures are identified predominantly in satellite glial cells of young and aging animals. Sensory neurons, as well as DRG macrophages, of the examined age groups do not contain connexin-43. When analyzing age-related changes in intercellular contacts in the DRG of rats, it was found that areas enriched with connexin-43, corresponding to plaques of protein channels that ensure metabolic interaction of satellite glial cells in the dorsal root ganglia, became more numerous with age. This fact may indicate activation of interaction between glial cells in the sensory ganglia during rat aging.
期刊介绍：
The journal publishes papers on vast aspects of cell research, including morphology, biochemistry, biophysics, genetics, molecular biology, immunology. The journal accepts original experimental studies, theoretical articles suggesting novel principles and approaches, presentations of new hypotheses, reviews highlighting major developments in cell biology, discussions. The main objective of the journal is to provide a competent representation and integration of research made on cells (animal and plant cells, both in vivo and in cell culture) offering insight into the structure and functions of live cells as a whole. Characteristically, the journal publishes articles on biology of free-living and parasitic protists, which, unlike Metazoa, are eukaryotic organisms at the cellular level of organization.
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