{"title":"The Influence of Perinatal Hypoxia (Asphyxia) on the Distribution of the α1 Subunit of the GABAA Receptor in the Neocortex of Newborn Rats","authors":"L. I. Khozhay","doi":"10.1134/s1990519x24010048","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The purpose of this work was to study the distribution of the α1 subunit of the GABA<sub>A</sub> receptor in the layers of the neocortex of rats in the neonatal period after exposure to hypoxia. The effect of hypoxia on the brain of newborn rats was carried out on the second neonatal day for 1 h at an oxygen content in the respiratory mixture of 7.8%. To identify the α1 subunit of GABA<sub>A</sub> receptor, immunohistochemical reaction was used. Protein content was assessed by the density of immunostaining of the reaction product in the cytoplasm and processes of neurons. We studied the somatosensory area of the neocortex at the fifth and tenth neonatal stages (P5, P10). It has been established that, in the early stages of the neonatal period, a significant population of young neurons is present in the neocortex, in the cytoplasm of which the α1 subunit, which is part of the GABA<sub>A</sub> receptor, is immunohistochemically detected. By the end of the neonatal period, in control animals, the density of staining of the immunohistochemical product of GABA<sub>A</sub> in the layers of the neocortex increases significantly. Exposure to perinatal hypoxia causes a reduction in the number of neurons containing the α1 subunit of the GABA<sub>A</sub> receptor and a significant decrease in the density of immunohistochemical staining in all layers of the neocortex.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"2013 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell and Tissue Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1134/s1990519x24010048","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
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Abstract
The purpose of this work was to study the distribution of the α1 subunit of the GABAA receptor in the layers of the neocortex of rats in the neonatal period after exposure to hypoxia. The effect of hypoxia on the brain of newborn rats was carried out on the second neonatal day for 1 h at an oxygen content in the respiratory mixture of 7.8%. To identify the α1 subunit of GABAA receptor, immunohistochemical reaction was used. Protein content was assessed by the density of immunostaining of the reaction product in the cytoplasm and processes of neurons. We studied the somatosensory area of the neocortex at the fifth and tenth neonatal stages (P5, P10). It has been established that, in the early stages of the neonatal period, a significant population of young neurons is present in the neocortex, in the cytoplasm of which the α1 subunit, which is part of the GABAA receptor, is immunohistochemically detected. By the end of the neonatal period, in control animals, the density of staining of the immunohistochemical product of GABAA in the layers of the neocortex increases significantly. Exposure to perinatal hypoxia causes a reduction in the number of neurons containing the α1 subunit of the GABAA receptor and a significant decrease in the density of immunohistochemical staining in all layers of the neocortex.
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The journal publishes papers on vast aspects of cell research, including morphology, biochemistry, biophysics, genetics, molecular biology, immunology. The journal accepts original experimental studies, theoretical articles suggesting novel principles and approaches, presentations of new hypotheses, reviews highlighting major developments in cell biology, discussions. The main objective of the journal is to provide a competent representation and integration of research made on cells (animal and plant cells, both in vivo and in cell culture) offering insight into the structure and functions of live cells as a whole. Characteristically, the journal publishes articles on biology of free-living and parasitic protists, which, unlike Metazoa, are eukaryotic organisms at the cellular level of organization.
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