A Study of the Effect of Glycodelin on the Level of T-Regulatory Lymphocytes and Acute Phase Proteins in Wistar Rats during the Administration of Allogenic Bone Marrow Cells

Abstract

The amniotic variant of glycodelin (GdA) has pronounced immunomodulatory properties, being involved in the formation of immune tolerance during pregnancy. The effect of GdA on the amount of T-regulatory lymphocytes (Treg) and the level of acute phase proteins (α-2-macroglobulin (α-2M), orosomucoid, C-reactive protein (CRP)) were studied during the administration of allogeneic red bone marrow (BM) cells to Wistar rats in a dynamic in vivo experiment. It was established that the administration of GdA against the background of the administration of allogeneic BM caused an increase in the portion of peripheral Tregs among CD4⁺ lymphocytes at the end of the experiment (on the 21st day) as compared with the group, which received BM. It was demonstrated that GdA decreased the level of CRP and α-2M, but increased the orosomucoid concentration in the serum of experimental animals at the beginning of the experiment (third day); however, normalization of the content of acute phase proteins to the level of intact animals was observed by the end of the experiment (21st day) in all groups of experimental animals. Thus, glycodelin is able to realize an immunosuppressive effect relative to the allogeneic cells through an increase in the level of Treg and orosomucoid, as well as a decrease in the concentration of CRP and α-2M.