Cell stem cell最新文献_第9页

Vascular architecture regulates mesenchymal stromal cell heterogeneity via P53-PDGF signaling in the mouse incisor 血管结构通过 P53-PDGF 信号调节小鼠门齿间充质基质细胞的异质性

IF 23.9 1区医学

Cell stem cell Pub Date : 2024-05-03 DOI: 10.1016/j.stem.2024.04.011

Tingwei Guo, Fei Pei, Mingyi Zhang, Takahiko Yamada, Jifan Feng, Junjun Jing, Thach-Vu Ho, Yang Chai

{"title":"Vascular architecture regulates mesenchymal stromal cell heterogeneity via P53-PDGF signaling in the mouse incisor","authors":"Tingwei Guo, Fei Pei, Mingyi Zhang, Takahiko Yamada, Jifan Feng, Junjun Jing, Thach-Vu Ho, Yang Chai","doi":"10.1016/j.stem.2024.04.011","DOIUrl":"https://doi.org/10.1016/j.stem.2024.04.011","url":null,"abstract":"Mesenchymal stem cells (MSCs) reside in niches to maintain tissue homeostasis and contribute to repair and regeneration. Although the physiological functions of blood and lymphatic vasculature are well studied, their regulation of MSCs as niche components remains largely unknown. Using adult mouse incisors as a model, we uncover the role of Trp53 in regulating vascular composition through THBS2 to maintain mesenchymal tissue homeostasis. Loss of Trp53 in GLI1+ progeny increases arteries and decreases other vessel types. Platelet-derived growth factors from arteries deposit in the MSC region and interact with PDGFRA and PDGFRB. Significantly, PDGFRA+ and PDGFRB+ cells differentially contribute to defined cell lineages in the adult mouse incisor. Collectively, our results highlight Trp53’s importance in regulating the vascular niche for MSCs. They also shed light on how different arterial cells provide unique cues to regulate MSC subpopulations and maintain their heterogeneity. Furthermore, they provide mechanistic insight into MSC-vasculature crosstalk.","PeriodicalId":9665,"journal":{"name":"Cell stem cell","volume":"87 1","pages":""},"PeriodicalIF":23.9,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140826427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Addressing challenges for repairing adult spinal cord with insights from neonates 从新生儿身上汲取灵感，应对修复成人脊髓的挑战

IF 23.9 1区医学

Cell stem cell Pub Date : 2024-05-02 DOI: 10.1016/j.stem.2024.04.008

Mickey E. Abraham, Joel Martin, Joseph D. Ciacci

引用次数: 0

Lumen expansion is initially driven by apical actin polymerization followed by osmotic pressure in a human epiblast model 在人类外胚层模型中，管腔扩张最初由顶端肌动蛋白聚合驱动，随后由渗透压驱动

IF 23.9 1区医学

Cell stem cell Pub Date : 2024-05-02 DOI: 10.1016/j.stem.2024.03.016

Dhiraj Indana, Andrei Zakharov, Youngbin Lim, Alexander R. Dunn, Nidhi Bhutani, Vivek B. Shenoy, Ovijit Chaudhuri

{"title":"Lumen expansion is initially driven by apical actin polymerization followed by osmotic pressure in a human epiblast model","authors":"Dhiraj Indana, Andrei Zakharov, Youngbin Lim, Alexander R. Dunn, Nidhi Bhutani, Vivek B. Shenoy, Ovijit Chaudhuri","doi":"10.1016/j.stem.2024.03.016","DOIUrl":"https://doi.org/10.1016/j.stem.2024.03.016","url":null,"abstract":"Post-implantation, the pluripotent epiblast in a human embryo forms a central lumen, paving the way for gastrulation. Osmotic pressure gradients are considered the drivers of lumen expansion across development, but their role in human epiblasts is unknown. Here, we study lumenogenesis in a pluripotent-stem-cell-based epiblast model using engineered hydrogels. We find that leaky junctions prevent osmotic pressure gradients in early epiblasts and, instead, forces from apical actin polymerization drive lumen expansion. Once the lumen reaches a radius of ∼12 μm, tight junctions mature, and osmotic pressure gradients develop to drive further growth. Computational modeling indicates that apical actin polymerization into a stiff network mediates initial lumen expansion and predicts a transition to pressure-driven growth in larger epiblasts to avoid buckling. Human epiblasts show transcriptional signatures consistent with these mechanisms. Thus, actin polymerization drives lumen expansion in the human epiblast and may serve as a general mechanism of early lumenogenesis.","PeriodicalId":9665,"journal":{"name":"Cell stem cell","volume":"84 1","pages":""},"PeriodicalIF":23.9,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140819887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alternative splicing of a chromatin modifier alters the transcriptional regulatory programs of stem cell maintenance and neuronal differentiation 染色质修饰因子的交替剪接改变了干细胞维持和神经元分化的转录调控程序

IF 23.9 1区医学

Cell stem cell Pub Date : 2024-05-02 DOI: 10.1016/j.stem.2024.04.001

Mohammad Nazim, Chia-Ho Lin, An-Chieh Feng, Wen Xiao, Kyu-Hyeon Yeom, Mulin Li, Allison E. Daly, Xianglong Tan, Ha Vu, Jason Ernst, Michael F. Carey, Stephen T. Smale, Douglas L. Black

{"title":"Alternative splicing of a chromatin modifier alters the transcriptional regulatory programs of stem cell maintenance and neuronal differentiation","authors":"Mohammad Nazim, Chia-Ho Lin, An-Chieh Feng, Wen Xiao, Kyu-Hyeon Yeom, Mulin Li, Allison E. Daly, Xianglong Tan, Ha Vu, Jason Ernst, Michael F. Carey, Stephen T. Smale, Douglas L. Black","doi":"10.1016/j.stem.2024.04.001","DOIUrl":"https://doi.org/10.1016/j.stem.2024.04.001","url":null,"abstract":"Development of embryonic stem cells (ESCs) into neurons requires intricate regulation of transcription, splicing, and translation, but how these processes interconnect is not understood. We found that polypyrimidine tract binding protein 1 (PTBP1) controls splicing of DPF2, a subunit of BRG1/BRM-associated factor (BAF) chromatin remodeling complexes. Dpf2 exon 7 splicing is inhibited by PTBP1 to produce the DPF2-S isoform early in development. During neuronal differentiation, loss of PTBP1 allows exon 7 inclusion and DPF2-L expression. Different cellular phenotypes and gene expression programs were induced by these alternative DPF2 isoforms. We identified chromatin binding sites enriched for each DPF2 isoform, as well as sites bound by both. In ESC, DPF2-S preferential sites were bound by pluripotency factors. In neuronal progenitors, DPF2-S sites were bound by nuclear factor I (NFI), while DPF2-L sites were bound by CCCTC-binding factor (CTCF). DPF2-S sites exhibited enhancer modifications, while DPF2-L sites showed promoter modifications. Thus, alternative splicing redirects BAF complex targeting to impact chromatin organization during neuronal development.","PeriodicalId":9665,"journal":{"name":"Cell stem cell","volume":"5 1","pages":""},"PeriodicalIF":23.9,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140819818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial metabolite steers intestinal stem cell fate under stress 微生物代谢物引导压力下的肠干细胞命运

IF 23.9 1区医学

Cell stem cell Pub Date : 2024-05-02 DOI: 10.1016/j.stem.2024.04.006

Shu Zhu, Wen Pan

引用次数: 0

The epidermal circadian clock integrates and subverts brain signals to guarantee skin homeostasis 表皮昼夜节律时钟整合并颠覆大脑信号，保证皮肤平衡

IF 23.9 1区医学

Cell stem cell Pub Date : 2024-05-02 DOI: 10.1016/j.stem.2024.04.013

Thomas Mortimer, Valentina M. Zinna, Muge Atalay, Carmelo Laudanna, Oleg Deryagin, Guillem Posas, Jacob G. Smith, Elisa García-Lara, Mireia Vaca-Dempere, Leonardo Vinícius Monteiro de Assis, Isabel Heyde, Kevin B. Koronowski, Paul Petrus, Carolina M. Greco, Stephen Forrow, Henrik Oster, Paolo Sassone-Corsi, Patrick-Simon Welz, Pura Muñoz-Cánoves, Salvador Aznar Benitah

{"title":"The epidermal circadian clock integrates and subverts brain signals to guarantee skin homeostasis","authors":"Thomas Mortimer, Valentina M. Zinna, Muge Atalay, Carmelo Laudanna, Oleg Deryagin, Guillem Posas, Jacob G. Smith, Elisa García-Lara, Mireia Vaca-Dempere, Leonardo Vinícius Monteiro de Assis, Isabel Heyde, Kevin B. Koronowski, Paul Petrus, Carolina M. Greco, Stephen Forrow, Henrik Oster, Paolo Sassone-Corsi, Patrick-Simon Welz, Pura Muñoz-Cánoves, Salvador Aznar Benitah","doi":"10.1016/j.stem.2024.04.013","DOIUrl":"https://doi.org/10.1016/j.stem.2024.04.013","url":null,"abstract":"In mammals, the circadian clock network drives daily rhythms of tissue-specific homeostasis. To dissect daily inter-tissue communication, we constructed a mouse minimal clock network comprising only two nodes: the peripheral epidermal clock and the central brain clock. By transcriptomic and functional characterization of this isolated connection, we identified a gatekeeping function of the peripheral tissue clock with respect to systemic inputs. The epidermal clock concurrently integrates and subverts brain signals to ensure timely execution of epidermal daily physiology. Timely cell-cycle termination in the epidermal stem cell compartment depends upon incorporation of clock-driven signals originating from the brain. In contrast, the epidermal clock corrects or outcompetes potentially disruptive feeding-related signals to ensure the optimal timing of DNA replication. Together, we present an approach for cataloging the systemic dependencies of daily temporal organization in a tissue and identify an essential gate-keeping function of peripheral circadian clocks that guarantees tissue homeostasis.","PeriodicalId":9665,"journal":{"name":"Cell stem cell","volume":"17 1","pages":""},"PeriodicalIF":23.9,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140821234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hallmarks of cancer stemness 癌症干细胞的特征

IF 23.9 1区医学

Cell stem cell Pub Date : 2024-05-02 DOI: 10.1016/j.stem.2024.04.004

Jia-Jian Loh, Stephanie Ma

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Actin pushes open a leaky lumen 肌动蛋白推开泄漏的管腔

IF 23.9 1区医学

Cell stem cell Pub Date : 2024-05-02 DOI: 10.1016/j.stem.2024.04.003

Jia Guo, Yue Shao

引用次数: 0

Sox9-coordinated cellular neighborhoods generate fibrosis Sox9 协调的细胞邻域产生纤维化