Cell stem cell最新文献_第9页

Human adult neurogenesis loss corresponds with cognitive decline during epilepsy progression 人类成人神经发生丧失与癫痫进展过程中的认知能力下降相对应

IF 23.9 1区医学

Cell stem cell Pub Date : 2024-12-05 DOI: 10.1016/j.stem.2024.11.002

Aswathy Ammothumkandy, Luis Corona, Kristine Ravina, Victoria Wolseley, Jeremy Nelson, Nadiya Atai, Aidin Abedi, Nora Jimenez, Michelle Armacost, Lina M. D'Orazio, Virginia Zuverza-Chavarria, Alisha Cayce, Carol McCleary, George Nune, Laura Kalayjian, Darrin J. Lee, Brian Lee, Robert H. Chow, Christianne Heck, Jonathan J. Russin, Michael A. Bonaguidi

{"title":"Human adult neurogenesis loss corresponds with cognitive decline during epilepsy progression","authors":"Aswathy Ammothumkandy, Luis Corona, Kristine Ravina, Victoria Wolseley, Jeremy Nelson, Nadiya Atai, Aidin Abedi, Nora Jimenez, Michelle Armacost, Lina M. D'Orazio, Virginia Zuverza-Chavarria, Alisha Cayce, Carol McCleary, George Nune, Laura Kalayjian, Darrin J. Lee, Brian Lee, Robert H. Chow, Christianne Heck, Jonathan J. Russin, Michael A. Bonaguidi","doi":"10.1016/j.stem.2024.11.002","DOIUrl":"https://doi.org/10.1016/j.stem.2024.11.002","url":null,"abstract":"Mesial temporal lobe epilepsy (MTLE) is a syndromic disorder presenting with seizures and cognitive comorbidities. Although seizure etiology is increasingly understood, the pathophysiological mechanisms contributing to cognitive decline and epilepsy progression remain less recognized. We have previously shown that adult hippocampal neurogenesis dramatically declines in MTLE patients with increased disease duration. Here, we investigate when multiple cognitive domains become affected during epilepsy progression and how human neurogenesis levels contribute to it. We find that intelligence, verbal learning, and memory decline at a critical period of 20 years disease duration. In contrast to rodents, the number of human immature neurons positively associates with auditory verbal, rather than visuospatial, learning and memory. Moreover, this association does not apply to mature granule neurons. Our study provides cellular evidence of how adult neurogenesis corresponds with human cognition and signifies an opportunity to advance regenerative medicine for patients with MTLE and other cognitive disorders.","PeriodicalId":9665,"journal":{"name":"Cell stem cell","volume":"36 1","pages":""},"PeriodicalIF":23.9,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142776745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Making blood from iPSCs: Reaching for the most sought-after prize 从多能干细胞中制造血液：追求最受欢迎的奖项

IF 23.9 1区医学

Cell stem cell Pub Date : 2024-12-05 DOI: 10.1016/j.stem.2024.11.008

Anna Bigas, Gayathri M. Kartha

引用次数: 0

Preventing radiation and chemo toxicity: Insights from bone marrow-on-a-chip 预防辐射和化学毒性：来自骨髓芯片的见解

IF 23.9 1区医学

Cell stem cell Pub Date : 2024-12-05 DOI: 10.1016/j.stem.2024.11.009

Milica Radisic

引用次数: 0

Stem cell islet replacement in type 1 diabetes: From “shelf” to “self” 1型糖尿病的干细胞胰岛替代：从“货架”到“自我”

IF 23.9 1区医学

Cell stem cell Pub Date : 2024-12-05 DOI: 10.1016/j.stem.2024.11.011

Eelco J.P. de Koning, Françoise Carlotti

引用次数: 0

Self-organization of the hematopoietic vascular niche and emergent innate immunity on a chip 芯片上造血血管生态位的自组织与突发先天免疫

IF 23.9 1区医学

Cell stem cell Pub Date : 2024-12-05 DOI: 10.1016/j.stem.2024.11.003

Andrei Georgescu, Joseph Hai Oved, Jonathan H. Galarraga, Thomas Cantrell, Samira Mehta, Brian M. Dulmovits, Timothy S. Olson, Pouria Fattahi, Anni Wang, Pelin L. Candarlioglu, Asli Muvaffak, Michele M. Kim, Sezin Aday Aydin, Jeongyun Seo, Eric S. Diffenderfer, Anthony Lynch, G. Scott Worthen, Dan Dongeun Huh

{"title":"Self-organization of the hematopoietic vascular niche and emergent innate immunity on a chip","authors":"Andrei Georgescu, Joseph Hai Oved, Jonathan H. Galarraga, Thomas Cantrell, Samira Mehta, Brian M. Dulmovits, Timothy S. Olson, Pouria Fattahi, Anni Wang, Pelin L. Candarlioglu, Asli Muvaffak, Michele M. Kim, Sezin Aday Aydin, Jeongyun Seo, Eric S. Diffenderfer, Anthony Lynch, G. Scott Worthen, Dan Dongeun Huh","doi":"10.1016/j.stem.2024.11.003","DOIUrl":"https://doi.org/10.1016/j.stem.2024.11.003","url":null,"abstract":"Here, we present a bioengineering approach to emulate the human bone marrow in vitro. Our developmentally inspired method uses self-organization of human hematopoietic stem and progenitor cells and vascular endothelial cells cultured in a three-dimensional microphysiological system to create vascularized, perfusable tissue constructs that resemble the hematopoietic vascular niche of the human marrow. The microengineered niche is capable of multilineage hematopoiesis and can generate functionally mature human myeloid cells that can intravasate into perfused blood vessels, providing a means to model the mobilization of innate immune cells from the marrow. We demonstrate the application of this system by presenting a specialized model of ionizing radiation-induced bone marrow injury and a multiorgan model of acute innate immune responses to bacterial lung infection. Furthermore, we introduce an advanced platform that enables large-scale integration and automated experimentation of the engineered hematopoietic tissues for preclinical screening of myelotoxicity due to anti-cancer drugs.","PeriodicalId":9665,"journal":{"name":"Cell stem cell","volume":"77 1","pages":""},"PeriodicalIF":23.9,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142776507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation of iPSC-derived human venous endothelial cells for the modeling of vascular malformations and drug discovery 生成 iPSC 衍生的人静脉内皮细胞，用于血管畸形建模和药物研发

IF 23.9 1区医学

Cell stem cell Pub Date : 2024-11-22 DOI: 10.1016/j.stem.2024.10.015

Zihang Pan, Qiyang Yao, Weijing Kong, Xiaojing Ma, Liangliang Tian, Yun Zhao, Shuntian Zhu, Sheng Chen, Mengze Sun, Jiao Liu, Simin Jiang, Jianxun Ma, Qijia Liu, Xiaohong Peng, Xiaoxia Li, Zixuan Hong, Yi Hong, Xue Wang, Jiarui Liu, Jingjing Zhang, Kai Wang

{"title":"Generation of iPSC-derived human venous endothelial cells for the modeling of vascular malformations and drug discovery","authors":"Zihang Pan, Qiyang Yao, Weijing Kong, Xiaojing Ma, Liangliang Tian, Yun Zhao, Shuntian Zhu, Sheng Chen, Mengze Sun, Jiao Liu, Simin Jiang, Jianxun Ma, Qijia Liu, Xiaohong Peng, Xiaoxia Li, Zixuan Hong, Yi Hong, Xue Wang, Jiarui Liu, Jingjing Zhang, Kai Wang","doi":"10.1016/j.stem.2024.10.015","DOIUrl":"https://doi.org/10.1016/j.stem.2024.10.015","url":null,"abstract":"Venous malformations (VMs) represent prevalent vascular anomalies typically attributed to non-inherited somatic mutations within venous endothelial cells (VECs). The lack of robust disease models for VMs impedes drug discovery. Here, we devise a robust protocol for the generation of human induced VECs (iVECs) through manipulation of cell-cycle dynamics via the retinoic signaling pathway. We introduce an L914F mutation into the TIE2 gene locus of induced pluripotent stem cells (iPSCs) and show that the mutated iVECs form dilated blood vessels after transplantation into mice, thereby recapitulating the phenotypic characteristics observed in VMs. Moreover, utilizing a deep neural network and a high-throughput digital RNA with perturbation of genes sequencing (DRUG-seq) approach, we perform drug screening and demonstrate that bosutinib effectively rescues the disease phenotype in vitro and in vivo. In summary, by leveraging genome editing and stem cell technology, we generate VM models that enable the development of additional therapeutics.","PeriodicalId":9665,"journal":{"name":"Cell stem cell","volume":"19 1","pages":""},"PeriodicalIF":23.9,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142690671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Post-transplant G-CSF impedes engraftment of gene-edited human hematopoietic stem cells by exacerbating p53-mediated DNA damage response 移植后的 G-CSF 通过加剧 p53 介导的 DNA 损伤反应，阻碍基因编辑的人类造血干细胞的移植

IF 23.9 1区医学

Cell stem cell Pub Date : 2024-11-12 DOI: 10.1016/j.stem.2024.10.013

Daisuke Araki, Vicky Chen, Neelam Redekar, Christi Salisbury-Ruf, Yan Luo, Poching Liu, Yuesheng Li, Richard H. Smith, Pradeep Dagur, Christian Combs, Andre Larochelle

{"title":"Post-transplant G-CSF impedes engraftment of gene-edited human hematopoietic stem cells by exacerbating p53-mediated DNA damage response","authors":"Daisuke Araki, Vicky Chen, Neelam Redekar, Christi Salisbury-Ruf, Yan Luo, Poching Liu, Yuesheng Li, Richard H. Smith, Pradeep Dagur, Christian Combs, Andre Larochelle","doi":"10.1016/j.stem.2024.10.013","DOIUrl":"https://doi.org/10.1016/j.stem.2024.10.013","url":null,"abstract":"Granulocyte-colony-stimulating factor (G-CSF) is commonly used to accelerate recovery from neutropenia following chemotherapy and autologous transplantation of hematopoietic stem and progenitor cells (HSPCs) for malignant disorders. However, its utility after ex vivo gene therapy in human HSPCs remains unexplored. We show that administering G-CSF from day 1 to 14 post-transplant impedes engraftment of CRISPR-Cas9 gene-edited human HSPCs in murine xenograft models. G-CSF affects gene-edited HSPCs through a cell-intrinsic mechanism, causing proliferative stress and amplifying the early p53-mediated DNA damage response triggered by Cas9-mediated DNA double-strand breaks. This underscores a threshold mechanism where p53 activation must reach a critical level to impair cellular function. Transiently inhibiting p53 or delaying the initiation of G-CSF treatment to day 5 post-transplant attenuates its negative impact on gene-edited HSPCs. The potential for increased HSPC toxicity associated with post-transplant G-CSF administration in CRISPR-Cas9 autologous HSPC gene therapy warrants consideration in clinical trials.","PeriodicalId":9665,"journal":{"name":"Cell stem cell","volume":"107 1","pages":""},"PeriodicalIF":23.9,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142599608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulated GATA1 expression as a universal gene therapy for Diamond-Blackfan anemia 将调控 GATA1 表达作为钻石-黑方贫血症的通用基因疗法

IF 23.9 1区医学

Cell stem cell Pub Date : 2024-11-11 DOI: 10.1016/j.stem.2024.10.012

Richard A. Voit, Xiaotian Liao, Alexis Caulier, Mateusz Antoszewski, Blake Cohen, Myriam Armant, Henry Y. Lu, Travis J. Fleming, Elena Kamal, Lara Wahlster, Aoife M. Roche, John K. Everett, Angelina Petrichenko, Mei-Mei Huang, William Clarke, Kasiani C. Myers, Craig Forester, Antonio Perez-Atayde, Frederic D. Bushman, Danilo Pellin, Vijay G. Sankaran

{"title":"Regulated GATA1 expression as a universal gene therapy for Diamond-Blackfan anemia","authors":"Richard A. Voit, Xiaotian Liao, Alexis Caulier, Mateusz Antoszewski, Blake Cohen, Myriam Armant, Henry Y. Lu, Travis J. Fleming, Elena Kamal, Lara Wahlster, Aoife M. Roche, John K. Everett, Angelina Petrichenko, Mei-Mei Huang, William Clarke, Kasiani C. Myers, Craig Forester, Antonio Perez-Atayde, Frederic D. Bushman, Danilo Pellin, Vijay G. Sankaran","doi":"10.1016/j.stem.2024.10.012","DOIUrl":"https://doi.org/10.1016/j.stem.2024.10.012","url":null,"abstract":"Gene therapy using hematopoietic stem and progenitor cells is altering the therapeutic landscape for patients with hematologic, immunologic, and metabolic disorders but has not yet been successfully developed for individuals with the bone marrow failure syndrome Diamond-Blackfan anemia (DBA). More than 30 mutations cause DBA through impaired ribosome function and lead to inefficient translation of the erythroid master regulator GATA1, providing a potential avenue for therapeutic intervention applicable to all patients with DBA, irrespective of the underlying genotype. Here, we report the development of a clinical-grade lentiviral gene therapy that achieves erythroid lineage-restricted expression of GATA1. We show that this vector is capable of augmenting erythropoiesis in DBA models and diverse patient samples without impacting hematopoietic stem cell function or demonstrating any signs of premalignant clonal expansion. These preclinical safety and efficacy data provide strong support for the first-in-human universal gene therapy trial for DBA through regulated GATA1 expression.","PeriodicalId":9665,"journal":{"name":"Cell stem cell","volume":"10 1","pages":""},"PeriodicalIF":23.9,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142599609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

All roads lead to cholesterol: Modulating lipid biosynthesis in multiple sclerosis patient-derived models 条条大路通罗马调节多发性硬化症患者衍生模型中的脂质生物合成

IF 23.9 1区医学

Cell stem cell Pub Date : 2024-11-07 DOI: 10.1016/j.stem.2024.10.011

Sophie Eichhorner, Larissa Traxler, Oliver Borgogno, Jerome Mertens

引用次数: 0

Nurturing protectors: Macrophages in the human pancreatic islet 培育保护者人类胰岛中的巨噬细胞

IF 23.9 1区医学

Cell stem cell Pub Date : 2024-11-07 DOI: 10.1016/j.stem.2024.10.006

Christopher Z.W. Lee, Francesca M. Spagnoli

引用次数: 0