Cell stem cell最新文献_第8页

Dopaminergic neurons entering the brain under the immunological cover of darkness 多巴胺能神经元在黑暗的免疫掩护下进入大脑

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-05-01 DOI: 10.1016/j.stem.2025.04.004

Timothy Chai, Julie Y. Chen, Kyle M. Loh

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Bioengineering innovations for neural organoids with enhanced fidelity and function 增强保真度和功能的类神经器官生物工程创新

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-05-01 DOI: 10.1016/j.stem.2025.03.014

Yubing Sun, Yoshiho Ikeuchi, Feng Guo, Insoo Hyun, Guo-li Ming, Jianping Fu

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Signaling switches: Metabolism regulates gastruloid self-organization 信号开关：代谢调节胃原质自组织

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-05-01 DOI: 10.1016/j.stem.2025.04.005

María J. Rodríguez Colman, Katharina F. Sonnen

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Roger A. Pedersen (1944–2025)

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-05-01 DOI: 10.1016/j.stem.2025.04.003

Victoria L. Mascetti

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Litigation of unlicensed US stem cell products 未经许可的美国干细胞产品的诉讼

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-05-01 DOI: 10.1016/j.stem.2025.04.001

Branden Lee, Noah Brookes, Adam S. Levin

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IRF6 hits the sweet spot IRF6达到了最佳点

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-05-01 DOI: 10.1016/j.stem.2025.04.002

Jane Reznick, Carien M. Niessen

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Induction of Multipotential Hematopoietic Progenitors from Human Pluripotent Stem Cells via Respecification of Lineage-Restricted Precursors 通过对谱系限制前体的再规范从人多能干细胞诱导多能造血祖细胞

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-04-29 DOI: 10.1016/j.stem.2025.04.009

Sergei Doulatov, Linda T. Vo, Stephanie S. Chou, Peter G. Kim, Natasha Arora, Hu Li, Brandon K. Hadland, Irwin D. Bernstein, James J. Collins, Leonard I. Zon, George Q. Daley

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Glycolytic activity instructs germ layer proportions through regulation of Nodal and Wnt signaling 糖酵解活性通过调节Nodal和Wnt信号传导来指导胚层比例

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-04-16 DOI: 10.1016/j.stem.2025.03.011

Kristina S. Stapornwongkul, Elisa Hahn, Patryk Poliński, Laura Salamó Palau, Krisztina Arató, LiAng Yao, Kate Williamson, Nicola Gritti, Kerim Anlas, Mireia Osuna Lopez, Kiran R. Patil, Idse Heemskerk, Miki Ebisuya, Vikas Trivedi

{"title":"Glycolytic activity instructs germ layer proportions through regulation of Nodal and Wnt signaling","authors":"Kristina S. Stapornwongkul, Elisa Hahn, Patryk Poliński, Laura Salamó Palau, Krisztina Arató, LiAng Yao, Kate Williamson, Nicola Gritti, Kerim Anlas, Mireia Osuna Lopez, Kiran R. Patil, Idse Heemskerk, Miki Ebisuya, Vikas Trivedi","doi":"10.1016/j.stem.2025.03.011","DOIUrl":"https://doi.org/10.1016/j.stem.2025.03.011","url":null,"abstract":"Metabolic pathways can influence cell fate decisions, yet their regulative role during embryonic development remains poorly understood. Here, we demonstrate an instructive role of glycolytic activity in regulating signaling pathways involved in mesoderm and endoderm specification. Using a mouse embryonic stem cell (mESC)-based <em>in vitro</em> model for gastrulation, we found that glycolysis inhibition increases ectodermal cell fates at the expense of mesodermal and endodermal lineages. We demonstrate that this relationship is dose dependent, enabling metabolic control of germ layer proportions through exogenous glucose levels. We further show that glycolysis acts as an upstream regulator of Nodal and Wnt signaling and that its influence on cell fate specification can be decoupled from its effects on growth. Finally, we confirm the generality of our findings using a human gastrulation model. Our work underscores the dependence of signaling pathways on metabolic conditions and provides mechanistic insight into the nutritional regulation of cell fate decision-making.","PeriodicalId":9665,"journal":{"name":"Cell stem cell","volume":"47 1","pages":""},"PeriodicalIF":23.9,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143837079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated molecular-phenotypic profiling reveals metabolic control of morphological variation in a stem-cell-based embryo model 综合分子表型分析揭示了干细胞胚胎模型中形态变异的代谢控制

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-04-16 DOI: 10.1016/j.stem.2025.03.012

Alba Villaronga-Luque, Ryan G. Savill, Natalia López-Anguita, Adriano Bolondi, Sumit Garai, Seher Ipek Gassaloglu, Roua Rouatbi, Kathrin Schmeisser, Aayush Poddar, Lisa Bauer, Tiago Alves, Sofia Traikov, Jonathan Rodenfels, Triantafyllos Chavakis, Aydan Bulut-Karslioglu, Jesse V. Veenvliet

{"title":"Integrated molecular-phenotypic profiling reveals metabolic control of morphological variation in a stem-cell-based embryo model","authors":"Alba Villaronga-Luque, Ryan G. Savill, Natalia López-Anguita, Adriano Bolondi, Sumit Garai, Seher Ipek Gassaloglu, Roua Rouatbi, Kathrin Schmeisser, Aayush Poddar, Lisa Bauer, Tiago Alves, Sofia Traikov, Jonathan Rodenfels, Triantafyllos Chavakis, Aydan Bulut-Karslioglu, Jesse V. Veenvliet","doi":"10.1016/j.stem.2025.03.012","DOIUrl":"https://doi.org/10.1016/j.stem.2025.03.012","url":null,"abstract":"Considerable phenotypic variation under identical culture conditions limits the potential of stem-cell-based embryo models (SEMs) in basic and applied research. The biological processes causing this seemingly stochastic variation remain unclear. Here, we investigated the roots of phenotypic variation by parallel recording of transcriptomic states and morphological history in individual structures modeling embryonic trunk formation. Machine learning and integration of time-resolved single-cell RNA sequencing with imaging-based phenotypic profiling identified early features predictive of phenotypic end states. Leveraging this predictive power revealed that early imbalance of oxidative phosphorylation and glycolysis results in aberrant morphology and a neural lineage bias, which we confirmed by metabolic measurements. Accordingly, metabolic interventions improved phenotypic end states. Collectively, our work establishes divergent metabolic states as drivers of phenotypic variation and offers a broadly applicable framework to chart and predict phenotypic variation in organoids and SEMs. The strategy can be used to identify and control underlying biological processes, ultimately increasing reproducibility.","PeriodicalId":9665,"journal":{"name":"Cell stem cell","volume":"1 1","pages":""},"PeriodicalIF":23.9,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143837080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing human iPSC-microglia for CNS-wide delivery of disease-modifying proteins 利用人类ipsc -小胶质细胞在全中枢神经系统范围内传递疾病修饰蛋白

IF 23.9 1区医学

Cell stem cell Pub Date : 2025-04-14 DOI: 10.1016/j.stem.2025.03.009

Jean Paul Chadarevian, Hayk Davtyan, Alina L. Chadarevian, Jasmine Nguyen, Joia K. Capocchi, Lauren Le, Adrian Escobar, Talar Chadarevian, Kimiya Mansour, Ekaterina Deynega, Michael Mgerian, Christina Tu, Sepideh Kiani Shabestari, William Carlen-Jones, Ghazaleh Eskandari-Sedighi, Jonathan Hasselmann, Robert C. Spitale, Mathew Blurton-Jones

{"title":"Harnessing human iPSC-microglia for CNS-wide delivery of disease-modifying proteins","authors":"Jean Paul Chadarevian, Hayk Davtyan, Alina L. Chadarevian, Jasmine Nguyen, Joia K. Capocchi, Lauren Le, Adrian Escobar, Talar Chadarevian, Kimiya Mansour, Ekaterina Deynega, Michael Mgerian, Christina Tu, Sepideh Kiani Shabestari, William Carlen-Jones, Ghazaleh Eskandari-Sedighi, Jonathan Hasselmann, Robert C. Spitale, Mathew Blurton-Jones","doi":"10.1016/j.stem.2025.03.009","DOIUrl":"https://doi.org/10.1016/j.stem.2025.03.009","url":null,"abstract":"Widespread delivery of therapeutic proteins to the brain remains challenging. To determine whether human induced pluripotent stem cell (iPSC)-microglia (iMG) could enable brain-wide and pathology-responsive delivery of therapeutic cargo, we utilized CRISPR gene editing to engineer iMG to express the Aβ-degrading enzyme neprilysin under control of the plaque-responsive promoter, CD9. To further determine whether increased engraftment enhances efficacy, we utilized a CSF1R-inhibitor resistance approach. Interestingly, both localized and brain-wide engraftment in Alzheimer’s disease (AD) mice reduced multiple biochemical measures of pathology. However, within the plaque-dense subiculum, reductions in plaque load, dystrophic neurites, and astrogliosis and preservation of neuronal density were only achieved following widespread microglial engraftment. Lastly, we examined chimeric models of breast cancer brain metastases and demyelination, demonstrating that iMG adopt diverse transcriptional responses to differing neuropathologies, which could be harnessed to enable widespread and pathology-responsive delivery of therapeutics to the CNS.","PeriodicalId":9665,"journal":{"name":"Cell stem cell","volume":"42 1","pages":""},"PeriodicalIF":23.9,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143827152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0