Cell stem cell最新文献_第7页

Human pluripotent stem cell-derived atrioventricular node-like pacemaker cells exhibit biological conduction bridge properties 人多能干细胞衍生的房室结样起搏器细胞表现出生物传导桥特性

IF 23.9 1区医学

Cell stem cell Pub Date : 2026-03-23 DOI: 10.1016/j.stem.2026.02.012

Michelle Lohbihler, Amos A. Lim, Stéphane Massé, Brandon M. Murareanu, Maggie Kwan, Omar Mourad, Olya Mastikhina, Malak Elbatarny, Renu Sarao, Beiping Qiang, Wahiba Dhahri, Matthew L. Chang, Alice L.Y. Xu, Kasinan Suthiwanich, Merrilyn Chen, Amine Mazine, Shahryar Khattak, Sara S. Nunes, Kumaraswamy Nanthakumar, Michael A. Laflamme, Stephanie Protze

引用次数: 0

Decoding the spatiotemporal development of the blood-brain barrier in human cortex 解码人类皮层血脑屏障的时空发展

IF 23.9 1区医学

Cell stem cell Pub Date : 2026-03-23 DOI: 10.1016/j.stem.2026.02.010

Zhongqiu Li, Yanxin Li, Ziqing He, Changliang Wang, Yuehong Zhang, Rong Li, Lei Jin, Jin Jiao, Fen Ji, Bing Zhu, Jingjing Zhang, Peng Du, Ji Dong, Jianwei Jiao

引用次数: 0

Auditory activity sustains adult neurogenesis and cognition through the locus coeruleus-norepinephrine system. 听觉活动通过蓝斑-去甲肾上腺素系统维持成人神经发生和认知。

IF 23.9 1区医学

Cell stem cell Pub Date : 2026-03-19 DOI: 10.1016/j.stem.2026.02.008

Qiang Liu,Xing Luo,Ziqi Liang,Dezhe Qin,Min Wang,Weixiang Guo

引用次数: 0

Paired patient-derived organoids reveal transcription factor-driven epigenetic remodeling in breast cancer metastasis 配对患者来源的类器官揭示了乳腺癌转移中转录因子驱动的表观遗传重塑

IF 23.9 1区医学

Cell stem cell Pub Date : 2026-03-16 DOI: 10.1016/j.stem.2026.02.007

Xinxin Rao, Jingwen Wang, Zhibin Qiao, Lei Hong, Mengxue Qiao, Liangwei Ni, Aixia Song, Yun Deng, Xu Zhao, Jin Meng, Xingxing Chen, Yifan Zhou, Jingyan Xue, Yayun Chi, Xinrui Wang, Zhaowei Yu, Qianqian Chen, Congling Xu, Shuang Tang, Jing Hu, Midie Xu, Wei Xu, Zhen Zhang, Yong Zhang, Yanhui Xu, Yi-Zhou Jiang, Jiong Wu, Minhong Shen, Xiaomao Guo, Xiaoli Yu, Fei Xavier Chen

{"title":"Paired patient-derived organoids reveal transcription factor-driven epigenetic remodeling in breast cancer metastasis","authors":"Xinxin Rao, Jingwen Wang, Zhibin Qiao, Lei Hong, Mengxue Qiao, Liangwei Ni, Aixia Song, Yun Deng, Xu Zhao, Jin Meng, Xingxing Chen, Yifan Zhou, Jingyan Xue, Yayun Chi, Xinrui Wang, Zhaowei Yu, Qianqian Chen, Congling Xu, Shuang Tang, Jing Hu, Midie Xu, Wei Xu, Zhen Zhang, Yong Zhang, Yanhui Xu, Yi-Zhou Jiang, Jiong Wu, Minhong Shen, Xiaomao Guo, Xiaoli Yu, Fei Xavier Chen","doi":"10.1016/j.stem.2026.02.007","DOIUrl":"https://doi.org/10.1016/j.stem.2026.02.007","url":null,"abstract":"Breast cancer exhibits marked clinical heterogeneity and dynamic epigenetic reprogramming during tumor progression, yet current subtyping approaches fail to capture molecular changes associated with metastasis. Here, we establish a comprehensive biobank of patient-derived organoids (PDOs) from matched primary tumors, adjacent normal tissues, and lymph node metastases. Integrated genomic, transcriptomic, and epigenetic analyses demonstrate that these PDOs preserve tumor-specific molecular signatures and recapitulate epigenetic remodeling during disease evolution. Epigenetic profiling defines four distinct clusters, characterized by unique transcription factor (TF) networks, pathway activities, and therapeutic vulnerabilities not fully represented by conventional classifications. The lymph node metastasis cluster, predominantly comprising metastatic PDOs, displays extensive chromatin remodeling driven by metastasis-enriched TFs, whose depletion markedly impairs spontaneous metastasis <ce:italic>in vivo</ce:italic>. Together, these findings establish PDO-based epigenetic characterization as a platform for elucidating regulatory mechanisms underlying breast cancer progression and for advancing precision therapeutic strategies.","PeriodicalId":9665,"journal":{"name":"Cell stem cell","volume":"11 1","pages":""},"PeriodicalIF":23.9,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147465833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vivo CAR-Tfh cell reprogramming restores tolerance in a mouse model of autoimmune hepatitis 体内CAR-Tfh细胞重编程恢复自身免疫性肝炎小鼠模型的耐受性

IF 23.9 1区医学

Cell stem cell Pub Date : 2026-03-13 DOI: 10.1016/j.stem.2026.02.006

Zuolin Zheng, Ying Liu, Ganyu Wang, Xinyuan Li, Shengxuan Peng, Zhen Jia, Xiao Yue, Zhipeng Fu, Xiaotian Zhao, Maosen Han, Zhenmei Yang, Chunwei Tang, Jinxin Gao, Zhichao Kong, Chen Chen, Kun Zhao, Tao Li, Xinyi Jiang, Weiqiang Jing

{"title":"In vivo CAR-Tfh cell reprogramming restores tolerance in a mouse model of autoimmune hepatitis","authors":"Zuolin Zheng, Ying Liu, Ganyu Wang, Xinyuan Li, Shengxuan Peng, Zhen Jia, Xiao Yue, Zhipeng Fu, Xiaotian Zhao, Maosen Han, Zhenmei Yang, Chunwei Tang, Jinxin Gao, Zhichao Kong, Chen Chen, Kun Zhao, Tao Li, Xinyi Jiang, Weiqiang Jing","doi":"10.1016/j.stem.2026.02.006","DOIUrl":"https://doi.org/10.1016/j.stem.2026.02.006","url":null,"abstract":"Autoimmune diseases involve the coordinated dysregulation of multiple types of immune cells, and single-cell targeted therapies often yield suboptimal results. Here, we developed chimeric antigen receptor (CAR)-engineered follicular helper T (Tfh) cells capable of simultaneously suppressing T cells, B cells, and dendritic cells (DCs) to restore immune homeostasis in an autoimmune hepatitis (AIH) mouse model. Using amino acid-derived lipid nanoparticles, we deliver self-amplifying RNA encoding Forkhead box protein P3 (Foxp3) and a cytochrome P4502D6 (CYP2D6)-specific CAR to Tfh cells, conferring stable regulatory features and antigen-dependent suppressive activity. Engineered CAR-Tfh cells preferentially localize to the liver, recognize CYP2D6-expressing hepatocytes, and suppress pathogenic T cell and B cell responses. In AIH-II mouse models, CAR-Tfh cell generation restores a tolerogenic hepatic immune environment and ameliorates autoimmune liver injury. These findings establish <em>in situ</em> Tfh cell reprogramming as a modular approach to coordinately modulate multiple immune compartments, providing a potential therapeutic framework for AIH and related autoimmune diseases.","PeriodicalId":9665,"journal":{"name":"Cell stem cell","volume":"19 1","pages":""},"PeriodicalIF":23.9,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147439684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling human prenatal adrenocortical functional zonation dynamics from pluripotent stem cells. 用多能干细胞模拟人产前肾上腺皮质功能分区动力学。

IF 23.9 1区医学

Cell stem cell Pub Date : 2026-03-05 DOI: 10.1016/j.stem.2026.02.002

Michinori Mayama,Eoin C Whelan,Takeshi Sato,David G Stouffer,Adrian N Leu,Jerome F Strauss,Richard J Auchus,Kotaro Sasaki

{"title":"Modeling human prenatal adrenocortical functional zonation dynamics from pluripotent stem cells.","authors":"Michinori Mayama,Eoin C Whelan,Takeshi Sato,David G Stouffer,Adrian N Leu,Jerome F Strauss,Richard J Auchus,Kotaro Sasaki","doi":"10.1016/j.stem.2026.02.002","DOIUrl":"https://doi.org/10.1016/j.stem.2026.02.002","url":null,"abstract":"The adrenal cortex produces essential steroid hormones through a concentric zonal architecture, established by the centripetal transdifferentiation of subcapsular progenitors within a capsule-derived niche. To capture this complexity, we establish a human pluripotent stem cell-derived adrenal organoid system that faithfully recapitulates this process. RSPO3/WNT signaling from the capsule specifies definitive zone (DZ) progenitors from the adrenal primordium, which then differentiate into a cortisol-producing transitional zone and an androgen-producing fetal zone under the influence of RSPO3 and ACTH. Loss of NR0B1 impairs DZ specification and triggers direct adrenal primordium-to-fetal zone conversion, mirroring the mechanism of X-linked adrenal hypoplasia congenita. When DZ cells are encapsulated with capsule cells separately derived from pluripotent stem cells, they reconstitute zonation in vivo, forming ACTH-responsive tissue that produces both cortisol and androgens. This organoid platform offers a powerful tool to dissect human adrenal development and establishes a foundation for regenerative therapies targeting adrenal diseases.","PeriodicalId":9665,"journal":{"name":"Cell stem cell","volume":"56 1","pages":"454-469.e9"},"PeriodicalIF":23.9,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147371019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adult neurogenesis: New neurons, new opportunities. 成人神经发生：新的神经元，新的机会。

IF 23.9 1区医学

Cell stem cell Pub Date : 2026-03-05 DOI: 10.1016/j.stem.2026.01.014

Birte Doludda,Warsha Barde,Francesco D'Egidio,Carlos P Fitzsimons,Jonas Frisén,Fred H Gage,Sebastian Jessberger,Orly Lazarov,D Chichung Lie,Paul J Lucassen,Chiara de Lucia,Evgenia Salta,Hongjun Song,Juan Song,Sandrine Thuret,Tomohisa Toda,Gerd Kempermann

引用次数: 0

Off-the-shelf CAR natural killer progenitor cell therapies are built to last. 现成的CAR自然杀伤祖细胞疗法是持久的。

IF 23.9 1区医学

Cell stem cell Pub Date : 2026-03-05 DOI: 10.1016/j.stem.2026.02.004

Noor Radde,Max Jan

引用次数: 0

Neuronal VIP shapes intestinal stem cell activity and mucosal immunity. 神经元VIP影响肠干细胞活性和粘膜免疫。

IF 23.9 1区医学

Cell stem cell Pub Date : 2026-03-05 DOI: 10.1016/j.stem.2026.02.001

Camilla Anastasio,Lucie Peduto

引用次数: 0

Tissues assemble! Rebuilding the human periportal liver in a dish. 组织组装!在培养皿中重建人类门静脉周围肝脏。