CARTILAGE最新文献

{"title":"Arthroscopic Debridement Combined With Platelet-Rich Plasma is a Viable Option for the Treatment of Advanced Ankle Osteoarthritis.","authors":"Taihei Miura, Yasuyuki Jujo, Jun Horng Tan, Chu Kai Man, Masato Takao","doi":"10.1177/19476035251378223","DOIUrl":"10.1177/19476035251378223","url":null,"abstract":"<p><p>ObjectiveTo assess the efficacy of arthroscopic debridement combined with platelet-rich plasma (PRP) therapy in treating advanced ankle osteoarthritis (OA).DesignThe data of 34 advanced ankle OA patients were retrospectively analyzed, with 17 patients undergoing arthroscopic debridement alone (No-PRP group) and 17 receiving debridement with PRP therapy (PRP group). The Self-Administered Foot Evaluation Questionnaire (SAFE-Q) was evaluated preoperatively and at 3, 6, and 12 months postoperatively. The complications were assessed throughout the follow-up period.ResultsIn the PRP group, all SAFE-Q subscales significantly improved at 6- and 12-month post-treatment compared with baseline (<i>P</i> < 0.05), whereas improvements in the No-PRP group were limited. For severe ankle OA patients, only the pain-related subscale of the SAFE-Q significantly improved from baseline in the No-PRP group (<i>P</i> < 0.05). In contrast, all SAFE-Q subscales demonstrated significant improvement at 12 months postoperatively in the PRP group (<i>P</i> < 0.05). The recurrence rates were 23.5% in the No-PRP group and 11.8% in the PRP group.ConclusionArthroscopic debridement combined with PRP therapy for advanced ankle OA significantly improved all SAFE-Q subscales postoperatively, whereas debridement alone had limited benefits. This combination may offer an effective treatment for advanced ankle OA.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"459-466"},"PeriodicalIF":2.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12449301/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145085074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interleukin-37 Ameliorates Articular Cartilage Damage in Two Murine Models of Osteoarthritis.","authors":"Ellen W van Geffen, Henk M van Beuningen, Joyce Aarts, Elly L Vitters, Wim H C Rijnen, Arjen B Blom, Fons A J van de Loo, Esmeralda N Blaney Davidson, Marije I Koenders, Arjan P M van Caam, Peter M van der Kraan","doi":"10.1177/19476035251372304","DOIUrl":"10.1177/19476035251372304","url":null,"abstract":"<p><p>ObjectiveIn this study, we investigated whether interleukin (IL)-37 ameliorates experimental osteoarthritis (OA).MethodsThe effects of IL-37 were investigated in collagenase-induced OA (CiOA) and destabilization of the medial meniscus (DMM). Human IL-37-adenovirus (ad-IL-37) was injected into the affected knee joint 4 and 18 days after the induction of OA. Luciferase-adenovirus was injected as control. Mice were sacrificed for histology at early and late stage of OA development. Interleukin-37 protein expression was confirmed by immunohistochemistry. Cartilage damage, osteophyte size and joint capsule thickness were measured. The effectiveness of ad-IL-37 was confirmed in vitro in human OA fibroblasts using real-time qualitative polymerase chain reaction (RT-qPCR) and Western blotting.ResultsInterleukin-37 protein expression was visible for at least 7 days after injection. At day 28, 10 days after the second injection, no clear synovial IL-37 staining was found any more, in both models. At day 28 of CiOA, ad-IL-37 significantly reduced articular cartilage damage and this was still reduced, although not significantly, at the late time point (day 42). In the DMM model, significant beneficial effect of IL-37 on cartilage damage was found at the late time point. In both OA models ad-IL-37 injections reduced the size of osteophytes.ConclusionInterleukin-37 ameliorated OA-induced articular cartilage damage and osteophyte formation in both models.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"808-820"},"PeriodicalIF":2.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12449308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145085039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arthroscopic Non-Concentrated Autologous Iliac Bone Marrow Stimulation Shows Stable Medium-Term Clinical and Radiological Outcomes for Small Cystic Talar Osteochondral Lesions.","authors":"Boyu Zheng, Fei Yan, Yanjun Zhong, Shijun Wei, Helin Wu, Feng Xu","doi":"10.1177/19476035251356380","DOIUrl":"10.1177/19476035251356380","url":null,"abstract":"<p><p>ObjectiveTo evaluate radiological, short-term, and medium-term clinical outcomes of arthroscopic non-concentrated iliac bone marrow stimulation (BMS) for small talar cystic osteochondral lesions of the talus (OLTs).DesignForty-three cases underwent this modified BMS between 2014 and 2019 were evaluated. Clinical outcomes were assessed by the Foot and Ankle Ability Measure (FAAM) Activities of Daily Living (ADL) and Sports Subscales (SS). Regenerated tissue was evaluated with the Magnetic Resonance Observation of Cartilage Repair Tissue scales (MOCART-2.0). Subgroup analysis based on locations and concomitant anterior talofibular ligament (ATFL) injuries.ResultsThe average diameter and depth of cysts were 6.97 ± 1.53 mm and 5.47 ± 1.10 mm, respectively. At a mean follow-up of 57.02 ± 19.61 months, FAAM-ADL and FAAM-SS improved significantly (45.65 ± 4.56 to 74.77 ± 8.03 and 12.63 ± 1.87 to 26.67 ± 3.41, respectively). From short-term to medium-term, FAAM-ADL revealed a minor decline (75.53 ± 7.76 vs. 74.77 ± 8.03, <i>P</i> = 0.421); FAAM-SS improved (25.37 ± 3.51 vs. 26.67 ± 3.41, <i>P</i> = 0.089). Medial lesions demonstrated favorable outcomes compared to lateral lesions [FAAM-ADL (77.04 ± 7.23 vs. 70.75 ± 8.10, <i>P</i> = 0.013), FAAM-SS (28.08 ± 2.40 vs. 24.19 ± 3.51, <i>P</i> < 0.001), and MOCART-2.0 (85.19 ± 11.27 vs. 71.88 ± 11.09, <i>P</i> < 0.001)]. Lateral lesions indicated higher rates of major hypertrophy (56.25% vs. 7.69%) and split-like defects (56.25% vs. 15.38%). The ATFL injuries did not significantly influence revision rates (15.8% vs. 4.2%, <i>P</i> = 0.439).ConclusionsArthroscopic non-concentrated iliac BMS demonstrated stable outcomes for small cystic OLTs. Lateral lesions were associated with inferior subjective scores and relatively higher rates of irregular fibrocartilage.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"427-437"},"PeriodicalIF":2.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12271133/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of Toll-Like Receptor 3 Relieves Osteoarthritis by Suppression of Cartilage Degradation, Nuclear Factor Kappa B-Mediated Inflammation, and Activation of Autophagy.","authors":"Zhe Hou, Mou Wang, Shun Cao","doi":"10.1177/19476035251317713","DOIUrl":"10.1177/19476035251317713","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the regulatory role of Toll-like receptor 3 (TLR3) in osteoarthritis (OA) progression, particularly its impacts on cartilage degradation, NF-κB-mediated inflammation, and autophagy activation.</p><p><strong>Method: </strong>1. Model Constuction: OA mouse model generated via anterior cruciate ligament transection (ACLT); LPS-induced inflammatory injury in murine ATDC5 chondrocytes; Histomorphological analysis of cartilage tissue using H&E and Safranine O staining. 2. Molecular Detection: TLR3 expression assessed by Western blot; Cartilage degradation markers (MMP-13, ADAMTS) and NF-κB pathway proteins analyzed via Western blot; Pro-inflammatory cytokine levels (IL-1β, TNF-α) quantified via RT-qPCR and Western blot. 3. Functional Assays: Cell viability examined via CCK-8 assay.</p><p><strong>Results: </strong>1. TLR3 Upregulation: TLR3 was highly expressed in OA cartilage and LPS-treated chondrocytes. 2. Cartilage Protection: TLR3 inhibition reduced cartilage erosion and proteoglycan loss in ACLT mice (confirmed by H&E and Safranine O staining); Downregulation of cartilage degradation markers (MMP-13, ADAMTS-5) observed in TLR3-knockdown models. 3. Anti-inflammatory Effects: TLR3 knockdown suppressed NF-κB activation, reducing IL-1β and TNF-α levels. 4. Autophagy Activation: Enhanced LC3-II/LC3-I ratio and Beclin-1 expression indicated TLR3 inhibition promotes autophagy.</p><p><strong>Conclusion: </strong>TLR3 drives OA progression through dual mechanisms: 1. Pro-inflammatory Pathway: Activates NF-κB signaling to amplify cytokine release and cartilage matrix breakdown. 2. Autophagy Suppression: Inhibits autophagy-related proteins, impairing cellular homeostasis. Targeting TLR3 may represent a therapeutic strategy to balance inflammation and autophagy, potentially slowing OA progression in multi-joint involvement cases.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"783-793"},"PeriodicalIF":2.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12187699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144483263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"KDM3A Modulates Biological Processes in Osteoarthritis Cell Models Via the Wnt/β-Catenin Signaling Pathway.","authors":"Yang Fu, Shixiong Yi, Qifeng Peng, Heng Jiang, Jie Zhou","doi":"10.1177/19476035251344878","DOIUrl":"10.1177/19476035251344878","url":null,"abstract":"<p><p>BackgroundOsteoarthritis (OA) is a chronic disease that seriously affects human health. Although biomarkers are vital to the discovery and therapy of OA, current research on OA-specific biomarkers remains limited, indicating a need for further expansion of this field of study.MethodsIn this study, differential genes in OA patients and normal samples in Genomics Expression Omnibus (GEO) database were analyzed for signaling pathway enrichment. Then, Weighted Gene Co-expression Network Analysis (WGCNA) combined with Least Absolute Shrinkage and Selection Operator (LASSO) analysis was used to obtain key genes associated with OA diagnosis, including BCL6 co-repressor (BCOR), Coiled-Coil Domain Containing 59 (CCDC59), Jun Proto-Oncogene (JUN), Lysine Demethylase 3A (KDM3A), L3MBTL Histone Methyl-Lysine Binding Protein 4 (L3MBTL4) and Zinc Finger Protein 292 (ZNF292). Finally, the role of KDM3A in OA cell model was verified by constructing KDM3A overexpression and silencing cell lines.ResultsIt was found that overexpression of KDM3A significantly downregulated β-catenin expression compared with the oe-NC group, thus affecting a series of biological processes in the OA cell model, specifically, increasing antioxidant capacity, reducing levels of inflammatory factors, and inhibiting extracellular matrix degradation.ConclusionThis study not only provided six key target genes for OA but also revealed the important role of KDM3A in OA, providing a reference for gene targeted therapy for OA patients.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"771-782"},"PeriodicalIF":2.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12182557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EUROVISCO Good Medical Practice Recommendations for the Use of Viscosupplementation with Hyaluronic Acid in the Management of Knee Osteoarthritis.","authors":"Jordi Monfort, Yves Henrotin, Xavier Chevalier, Alberto Migliore, Dominique Baron, Jörg Jerosch, Pascal Richette, Hervé Bard, Mats Brittberg, Demirhan Diraçoglù, Raghu Raman, Thierry Conrozier","doi":"10.1177/19476035241286578","DOIUrl":"10.1177/19476035241286578","url":null,"abstract":"<p><strong>Objective: </strong>Viscosupplementation (VS) by intra-articular injections of hyaluronic acid (HA) is a commonly used treatment of knee osteoarthritis. However, there are no clear recommendations for its use in clinical situations not usually addressed in therapeutic guidelines.</p><p><strong>Design: </strong>Members of the European Viscosupplementation Consensus Group (EUROVISCO), made up of rheumatologists, orthopedic surgeons, and rehabilitation physicians from seven European countries, were asked to make a therapeutic decision on 30 clinical scenarios based on their clinical experience and data from the literature, using the Delphi method. For each scenario, the strength of agreement and the level of consensus were calculated by the chairman of the group.</p><p><strong>Results: </strong>The expert panel reached consensus on 18 of the 30 clinical scenarios proposed. According to the experts, the factors that most influence the decision to use VS with HA to treat knee osteoarthritis are the nature and severity of symptoms, the presence of comorbidity, the absence of therapeutic alternatives, and the patient's refusal to undergo knee arthroplasty. Obesity and an advanced radiological degree of osteoarthritis have been identified as the two main factors for poor response to VS with HA and should be considered before any decision is made to viscosupplement.</p><p><strong>Conclusion: </strong>This set of recommendations, based on common clinical scenarios, is intended to help practitioners make decisions about HA VS in patients with osteoarthritis of the knee.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"322-330"},"PeriodicalIF":2.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954159/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calcium-Phosphate Subchondroplasty Provides Durable 24-Month Pain Relief and >90% Surgery-free Survivorship for Tibiofemoral Bone Marrow Lesions in Mild-To-Moderate Knee Osteoarthritis: A Prospective Multicenter Study.","authors":"Giuseppe Anzillotti, Elizaveta Kon, Pietro Conte, Peter Angele, Pieter Emans, Joan Minguell-Monyart, Jennifer Woodell-May, Miguel Correa-Tapia, Berardo Di Matteo","doi":"10.1177/19476035261476205","DOIUrl":"10.1177/19476035261476205","url":null,"abstract":"<p><p>PurposeTo report 24-month clinical outcomes, surgery-free survivorship, and radiological variations after Calcium-Phosphate (CaP) Subchondroplasty (SCP) procedures in patients with symptomatic knee bone marrow lesions (BMLs) in the context of mild-to-moderate knee osteoarthritis (OA).MethodsAdults with tibiofemoral BMLs (Kellgren-Lawrence [KL] 1-3) underwent CaP SCP using a standardized protocol. Prespecified outcomes were KOOS (primary: KOOS Pain), EQ-5D-5L, and pain NRS at baseline, 1, 3, 6, 12, and 24 months; surgery-free survivorship (Kaplan-Meier); and structural status by KL and MOAKS (available-case imaging). Analyses compared time points with paired tests and 95% CIs.ResultsNinety-two knees were treated. KOOS Pain improved from 45.9 ± 14.3 preoperatively to 81.3 ± 18.1 at 24 months (<i>p</i> < .0001). All other KOOS subscales improved from baseline to 24 months (all <i>p</i> < .0001) and pain NRS decreased from 6.8 ± 1.5 to 3.3 ± 2.6 (<i>p</i> < .0001). Kaplan-Meier surgery-free survivorship was 97.7% (95% CI, 90.9-99.4) at 12 months and 91.5% (95% CI, 82.9-95.8) at 24 months; 7/92 knees (7.6%) underwent reoperation. KL distributions showed no significant change versus baseline at 12 months (<i>p</i> = .5089) or 24 months (<i>p</i> = .9801). MOAKS improved at 12 months (<i>p</i> = .0435) but not at 24 months (<i>p</i> = .2517) in the available MRI subset. No serious device-related adverse events were observed.ConclusionIn BML-positive knees with KL 1-3 OA, SCP yielded large and durable improvements in pain, function, and health-related quality of life through 24 months, with >90% surgery-free survivorship and radiographic stability.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035261476205"},"PeriodicalIF":2.6,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13530047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148863761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-Cell Transcriptomic Profiling Reveals Superficial Zone Chondrocytes as a Catabolic and Senescent Focus in Osteoarthritis Cartilage.","authors":"Yoonhee Kim, Gaeun Jung, Seungwoo Han","doi":"10.1177/19476035261481078","DOIUrl":"10.1177/19476035261481078","url":null,"abstract":"<p><p>ObjectiveTo compare zone-specific expression patterns of catabolic proteases and senescence-related genes across chondrocyte subpopulations in osteoarthritis (OA) cartilage.DesignSingle-cell RNA sequencing data from 72,616 chondrocytes (11 human cartilage samples; 6 normal, 5 OA; GSE220243) were analyzed. Following quality control and Harmony-based batch correction, cells were clustered into 27 subpopulations and assigned to superficial (SZ), middle (MZ), deep (DZ), or calcified (CZ) zones using zone-specific gene set module scores. Developmental origin scoring, catabolic gene expression ranking, senescence scoring, and signaling pathway analysis were performed across all clusters.ResultsUpon zonal assignment, the MZ constituted the largest compartment (73.6%), followed by the SZ (21.9%) and CZ (4.5%), with no cluster assigned a dominant DZ identity. MMP13 and ADAMTS5 showed their highest per-cell expression in SZ clusters, with MMP13 most prominent in a FibroC-COL1A2⁺ superficial cluster (c10). SZ chondrocytes harbored a constitutively encoded fibroblastic identity transcriptionally resembling perichondrial and synovial fibroblast programs, independent of OA status, with concurrent deficiencies in Wnt inhibitor (FRZB, DKK1, WIF1) and FGF chondroprotective signaling relative to MZ. Senescence was observed in terminally differentiated clusters of both SZ and MZ, where a constitutively senescence-prone niche in SZ associated with mTOR and HIF1A signaling was mechanistically distinct from the senescence program observed in the MZ clusters.ConclusionsThese findings identify the developmentally distinct SZ as a candidate catabolic and senescent focus in OA cartilage and a potential contributor to matrix degradation and target for zone-stratified therapeutic intervention.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035261481078"},"PeriodicalIF":2.6,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13518615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148825195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intra-Articular miR-199a-5p Attenuates Post-traumatic Ankle Cartilage Degeneration With Reduced IHH Expression and Hypertrophic-Catabolic Marker Expression.","authors":"Zhihong Fu, Yunhui Zhang, Hui Guo, Ruizheng Zhu, Zunwang Li, Dongxiao Li, Xingjie Mo, Shaojia Chen, Changren Bao, Zhongwen Lu, Fangyuan Wei, Zhaojun Chen","doi":"10.1177/19476035261482024","DOIUrl":"10.1177/19476035261482024","url":null,"abstract":"<p><p>BackgroundPost-traumatic ankle osteoarthritis (TAA) is driven by injury-related inflammation, cartilage matrix loss, and chondrocyte phenotypic instability. Indian hedgehog (IHH) is implicated in hypertrophic and catabolic cartilage remodeling, but whether local miR-199a-5p administration is associated with changes in IHH-related expression patterns in traumatic ankle cartilage remains incompletely defined.MethodsForty-eight male Sprague-Dawley rats were randomized to sham, TAA model, miR-199a-5p agomir, or negative-control agomir groups. TAA-like cartilage degeneration was induced by medial malleolar fracture followed by unrestricted cage activity. From week 2, rats received weekly intra-articular saline, miR-199a-5p agomir, or negative-control agomir for six weeks. Longitudinal inflammation, function, and ankle circumference ratio were assessed, followed by radiography, histology, OARSI scoring, ELISA, Western blotting, and RT-qPCR at week 8.ResultsThe fracture model caused persistent swelling and functional impairment, radiographic post-traumatic changes, cartilage surface disruption, proteoglycan depletion, increased IL-1β/TNF-α production, increased IHH/RUNX2/MMP-13 expression, and reduced SOX9/COL2A1 expression. miR-199a-5p agomir treatment partially improved function, reduced inflammatory scores and cytokine levels, lowered OARSI scores, and was associated with lower IHH expression and a less hypertrophic/catabolic marker profile. The negative-control agomir showed no comparable protective pattern.ConclusionsIntra-articular miR-199a-5p agomir attenuated experimental TAA-like cartilage degeneration. The protective phenotype was associated with reduced inflammation, lower IHH expression, attenuation of hypertrophic/catabolic marker expression, and partial restoration of cartilage matrix-related markers. Direct miR-199a-5p binding to IHH, downstream GLI activation, and local miRNA tissue accumulation were not tested and require further study.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035261482024"},"PeriodicalIF":2.6,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13503636/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148808345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Textural Features from Quantitative MRI Using T2 Relaxation Times Correlate with Histological Cartilage Degeneration: An Ex-Vivo Study.","authors":"Markus Schreiner, Stefan Toegel, Veronika Janacova, Pavol Szomolanyi, Didier Laurent, Franziska Saxer, Matthias Schieker, Rahel Heule, Oliver Bieri, Esther Raithel, Christoph Fuchssteiner, Wolfgang J Weninger, Reinhard Windhager, Siegfried Trattnig, Benedikt Hager, Vladimir Juras","doi":"10.1177/19476035261478556","DOIUrl":"https://doi.org/10.1177/19476035261478556","url":null,"abstract":"<p><p>ObjectiveTo assess the relationship between textural features derived from quantitative MRI (T₂ mapping) and histological signs of cartilage degeneration.DesignTen fresh non-frozen knees from body donors were measured at 7T and 3T scanners using 3D-Double Echo Steady State (3D-DESS) as well as 3D-Triple Echo Steady State (3D-TESS) sequence. The 3D-DESS images were automatically segmented and co-registered with T₂ maps at both field strengths. Nine osteochondral plugs were harvested from standardized regions of the distal femur of each knee. The histological workup included Safranin O and picrosirius red staining. Mankin scoring was performed for each plug. Collagen orientation and parallelism were assessed and quantified based on polarized light microscopy. Each plug was segmented on the 3D model derived from 3D-DESS image and texture analysis was performed on the co-registered T₂ map. Relationships between T₂ relaxation time, textural features and the Mankin score, as well as collagen orientation and parallelism, were assessed using partial Spearman's correlation coefficients and mixed models.ResultsMean T₂ and six features (autocorrelation, contrast, correlation, entropy, sum entropy and inverse difference moment normalized) correlated with the MS; autocorrelation, contrast and entropy were also found to be correlated with collagen orientation. No features were significantly correlated with parallelism. Statistical models showed no significant effect of sequence or field strength in predicting Mankin score.ConclusionsThe presented ex-vivo approach enables a semi-automated assessment of articular cartilage quality that correlates with histological measures of degeneration. Additional studies are warranted to further investigate the reproducibility and validity of the measurements.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035261478556"},"PeriodicalIF":2.6,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13493633/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148788801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}