基于新的显微镜方法的膝关节软骨弹性模量降低准确地反映了退化的早期组织学发现。

IF 2.7 4区 医学 Q1 ORTHOPEDICS
CARTILAGE Pub Date : 2024-12-01 Epub Date: 2023-09-01 DOI:10.1177/19476035231194770
Takahiro Maeda, Shinichi Kuriyama, Shigeo Yoshida, Kohei Nishitani, Shinichiro Nakamura, Shuichi Matsuda
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引用次数: 0

摘要

目的:据报道,体内纳米压痕测量表明,弹性模量会随着软骨的退化而降低,但目前还没有一种方法能对体内机械性能进行宏观评估。本研究的目的是根据宏观方法评估膝关节软骨的弹性模量,并将其与退化的大体和组织学结果进行比较:骨软骨切片取自 50 个接受全膝关节置换术的骨关节炎膝关节(平均年龄 75 岁)。用专门的弹性测试仪测量软骨的弹性模量。大体检查结果按国际软骨修复学会(ICRS)分级记录。组织学检查结果按Mankin评分和显微镜下软骨厚度测量进行分级:结果:在国际软骨修复学会(ICRS)0至2级的正常至中度软骨异常膝关节中,随着软骨退化的进展,软骨的弹性模量明显下降。ICRS 0 级软骨的弹性模量为 12.2 ± 3.8 N/mm,ICRS 1 级为 6.3 ± 2.6 N/mm,ICRS 2 级为 3.8 ± 2.4 N/mm。同样,弹性模量也与 Mankin 评分相关(r = -0.51,P < 0.001)。多元回归分析表明,Mankin评分的增加是与软骨弹性模量下降最相关的因素(t值,-4.53;P < 0.001),其次是软骨组织学厚度的增加(t值,-3.15;P = 0.002):结论:用新的宏观方法评估受损膝关节软骨的力学性能与组织学结果密切相关。该方法有望成为临床上早期软骨损伤的无损诊断方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Decreased Elastic Modulus of Knee Articular Cartilage Based on New Macroscopic Methods Accurately Represents Early Histological Findings of Degeneration.

Objective: Ex vivo nanoindentation measurement has reported that elastic modulus decreases as cartilage degenerates, but no method has been established to macroscopically evaluate mechanical properties in vivo. The objective of this study was to evaluate the elastic modulus of knee joint cartilage based on macroscopic methods and to compare it with gross and histological findings of degeneration.

Design: Osteochondral sections were taken from 50 knees with osteoarthritis (average age, 75 years) undergoing total knee arthroplasty. The elastic modulus of the cartilage was measured with a specialized elasticity tester. Gross findings were recorded as International Cartilage Repair Society (ICRS) grade. Histological findings were graded as Mankin score and microscopic cartilage thickness measurement.

Results: In ICRS grades 0 to 2 knees with normal to moderate cartilage abnormalities, the elastic modulus of cartilage decreased significantly as cartilage degeneration progressed. The elastic modulus of cartilage was 12.2 ± 3.8 N/mm for ICRS grade 0, 6.3 ± 2.6 N/mm for ICRS grade 1, and 3.8 ± 2.4 N/mm for ICRS grade 2. Similarly, elastic modulus was correlated with Mankin score (r = -0.51, P < 0.001). Multiple regression analyses showed that increased Mankin score is the most relevant factor associated with decreased elastic modulus of the cartilage (t-value, -4.53; P < 0.001), followed by increased histological thickness of the cartilage (t-value, -3.15; P = 0.002).

Conclusions: Mechanical properties of damaged knee cartilage assessed with new macroscopic methods are strongly correlated with histological findings. The method has potential to become a nondestructive diagnostic modality for early cartilage damage in the clinical setting.

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来源期刊
CARTILAGE
CARTILAGE ORTHOPEDICS-
CiteScore
6.90
自引率
7.10%
发文量
80
期刊介绍: CARTILAGE publishes articles related to the musculoskeletal system with particular attention to cartilage repair, development, function, degeneration, transplantation, and rehabilitation. The journal is a forum for the exchange of ideas for the many types of researchers and clinicians involved in cartilage biology and repair. A primary objective of CARTILAGE is to foster the cross-fertilization of the findings between clinical and basic sciences throughout the various disciplines involved in cartilage repair. The journal publishes full length original manuscripts on all types of cartilage including articular, nasal, auricular, tracheal/bronchial, and intervertebral disc fibrocartilage. Manuscripts on clinical and laboratory research are welcome. Review articles, editorials, and letters are also encouraged. The ICRS envisages CARTILAGE as a forum for the exchange of knowledge among clinicians, scientists, patients, and researchers. The International Cartilage Repair Society (ICRS) is dedicated to promotion, encouragement, and distribution of fundamental and applied research of cartilage in order to permit a better knowledge of function and dysfunction of articular cartilage and its repair.
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