Burns & Trauma最新文献

{"title":"Fibroblast Exosomes Promote Wound Healing and Improve the Quality of Healed Skin via miR-29a-3p-Mediated KEAP1/Nrf2 Pathway Activation","authors":"Lingfeng Yan, Dejiang Fan, Jiacai Yang, Jue Wang, Xiaohong Hu, Xiaorong Zhang, Yong Huang, Hong Wang, Wenjing Yin, Xin Cai, Ruoyu Shang, Canhua Huang, Gaoxing Luo, Weifeng He","doi":"10.1093/burnst/tkaf035","DOIUrl":"https://doi.org/10.1093/burnst/tkaf035","url":null,"abstract":"Background Wound healing is a sophisticated biological process characterized by the orchestrated interplay of diverse cellular components, growth factors, and signaling cascades. Recent research has highlighted the pivotal role of fibroblast exosomes in mediating intercellular communication and facilitating tissue regeneration. This investigation aimed to elucidate the therapeutic efficacy of fibroblast exosomes in enhancing wound repair mechanisms, with a particular emphasis on their differential effects in normal and diabetic wound healing paradigms. Methods A mouse full-thickness skin defect model was used to evaluate the effects of fibroblast exosomes on wound re-epithelialization, granulation tissue formation, and epidermal barrier function. Molecular and cellular experiments were conducted to analyze the roles of exosomes in epidermal stem cell proliferation, migration, differentiation, and antioxidant stress, with further validation of the associated signaling pathways. The therapeutic efficacy was additionally confirmed in a type 1 diabetic mouse model. Results Fibroblast exosomes significantly enhanced wound re-epithelialization by promoting the proliferation, migration, and differentiation of epidermal stem cells. Additionally, exosomes increased fibroblast abundance and myofibroblast activation, facilitating granulation tissue formation as well as improving extracellular matrix (ECM) deposition and the biomechanical properties of healed skin. Furthermore, exosomes improved epidermal barrier function by upregulating tight junction proteins (e.g., Claudin-1 and ZO-1) and reducing transepidermal water loss (TEWL). In diabetic mouse models, exosomes accelerated wound closure, restored ECM deposition and biomechanical integrity, and repaired epidermal barrier function. Mechanistically, exosomes target the 3′ untranslated region (UTR) of Keap1 mRNA through miR-29a-3p and activate the KEAP1/Nrf2 antioxidant pathway, mitigating oxidative stress and protecting epidermal stem cells from reactive oxygen species (ROS)-induced damage. Conclusion Fibroblast exosomes alleviate oxidative damage by modulating the KEAP1/Nrf2 pathway through miR-29a-3p and enhancing epidermal stem cell function. These exosomes exhibit remarkable therapeutic potential in accelerating wound healing and improving healing quality under both normal and diabetic conditions, offering a robust foundation for innovative therapeutic strategies.","PeriodicalId":9553,"journal":{"name":"Burns & Trauma","volume":"1 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144083180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring machine learning strategies for single-cell transcriptomic analysis in wound healing","authors":"Jianzhou Cui, Mei Wang, Chenshi Lin, Xu Xu, Zhenqing Zhang","doi":"10.1093/burnst/tkaf032","DOIUrl":"https://doi.org/10.1093/burnst/tkaf032","url":null,"abstract":"Wound healing is a highly orchestrated, multi-phase process that involves various cell types and molecular pathways. Recent advances in single-cell transcriptomics and machine learning have provided unprecedented insights into the complexity of this process, enabling the identification of novel cellular subpopulations and molecular mechanisms underlyingtissue repair. In particular, single-cell RNA sequencing (scRNA-seq) has revealedsignificant cellular heterogeneity, especially withinfibroblast populations, and has provided valuable information on immune cell dynamics during healing. Machine learning algorithms have enhanced data analysis by improving cell clustering, dimensionality reduction, and trajectory inference, leading to a better understanding of wound healing at the single-cell level. This review synthesizes the latest findings on the application of scRNA-seq and machine learning in wound healing research, with a focus on fibroblast diversity, immune responses, and spatial organization of cells. The integration of these technologies has the potential to revolutionize therapeutic strategies for chronic wounds, fibrosis, and tissue regeneration, offering new opportunities for precision medicine. By combining computational approacheswith biological insights, this review highlights the transformative impact of scRNA-seq and machine learning on wound healing research.","PeriodicalId":9553,"journal":{"name":"Burns & Trauma","volume":"231 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143946102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular vesicles and their mimetics: clinical application prospects in medical aesthetics","authors":"Zixuan Sun, Jiali Li, Zhijing Wu, Jiaxin Zhang, Tangrong Wang, Yuzhou Zheng, XueZhong Xu, Wei Ding, Hui Qian, Yulin Tan","doi":"10.1093/burnst/tkaf033","DOIUrl":"https://doi.org/10.1093/burnst/tkaf033","url":null,"abstract":"The persistent desire for beauty has fueled the rapid development of medical aesthetics. Multiple approaches, including cosmetics, drugs, and cell therapies, have been developed to improve skin conditions. However, none of these methods achieves the intended outcomes and may produce adverse repercussions. Consequently, it is crucial to develop effective and safe therapies to promote skin repair and regeneration. Extracellular vesicles (EVs) are cell-derived, lipid bilayer-encapsulated nanoparticles containing multiple active components. There is now strong evidence that EV therapies can improve skin barrier function, reduce scarring, inhibit aging and pigmentation, and suppress hair loss, highlighting the potential for cosmeceutical development based on EVs. This review summarizes the latest applications of natural EVs, preconditioned and engineered EVs, and EV mimetics in medical aesthetics. Additionally, the article discusses advanced delivery strategies for EVs and the commercial prospects of EVs and provides insights into present security concerns, regulations, and the field’s future directions.","PeriodicalId":9553,"journal":{"name":"Burns & Trauma","volume":"16 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144083183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrospun Nanofiber Scaffolds Loaded with Copper Oxide for Repairing Traumatic Brain Injury through Restoring Copper Homeostasis and Regulating Pyroptosis pathway","authors":"Yumei An, Sunao Li, Xinqi Huang, Xueshi Chen, Mingyuan Xu, Chen Chen, Xuefeng Zhou, Haiyan Shan, Luyang Tao, Mingyang Zhang","doi":"10.1093/burnst/tkaf030","DOIUrl":"https://doi.org/10.1093/burnst/tkaf030","url":null,"abstract":"Background TBI is one of the leading causes of injury and disability worldwide. Pyroptosis, a specific type of programmed cell death (PCD) triggered by inflammatory signals, plays a significant part in the pathological process after TBI. Copper ions play an important role in anti-inflammation and anti-oxidative stress. There is a more active copper metabolism in neurons after injury, and that neurons may require more copper ions and downstream copper-based enzymes to maintain normal physiological functions. Methods We developed an electrostatic spinning scaffold loaded with copper oxide (CuO@ PCL/gelatin) to achieve small-dose local administration and avoid toxic side effects. The membranes underwent preparation and characterization through various techniques including Fourier transform infrared spectroscopy, measurement of water contact angle, antibacterial experiment, scanning electron microscopy, and assessment of in vitro release of copper. In addition, we used a controlled cortical impact (CCI) to establish a TBI model in mice to examine the effect of CuO@PG on TBI-induced pyroptosis and the ability of the membranes to heal brain injury. Results CuO@PG inhibited TBI-induced neuronal pyroptosis. CuO@PG can inhibit the expression of the pyroptosis-related proteins. Moreover, CuO@PG also alleviates brain edema and the degree of neurodegeneration in the acute phase of TBI. The neuroprotective effect of CuO@PG was further confirmed by wire-grip test, open field test, Morris water maze test. Lastly, the beneficial results were significantly inhibited by the use of the copper chelator TTM. Conclusions In this study, we successfully constructed electrostatically spun scaffolds loaded with copper oxide to achieve slow, continuous and low-dose copper supply to the local brain, which provides a new theoretical basis for the imbalance of copper homeostasis in the brain after TBI.","PeriodicalId":9553,"journal":{"name":"Burns & Trauma","volume":"3 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organoids/organs-on-chips towards biomimetic human artificial skin","authors":"Yuting Huang, Xiaoyan Wu, Yongxin Xu, Nengjie Yang, Peipei Xi, Yunan Wang, Yujuan Zhu, Xiaodong Chen","doi":"10.1093/burnst/tkaf029","DOIUrl":"https://doi.org/10.1093/burnst/tkaf029","url":null,"abstract":"As the largest organ in the human body, the skin protects the body from pathogens and harmful substances through physical, chemical and immune barrier functions. However, accurately replicating the complex physiology of human skin in mouse models remains a significant challenge. Accurately replicating the complex physiology of human skin in mouse models remains a significant challenge, making the development of bionic artificial skin particularly important. In recent years, skin organoid and skin-on-a-chip technologies have greatly enhanced in vitro skin modeling, overcoming many limitations of traditional approaches. In this review, we comprehensively summarize important advances in research on skin organoids and skin-on-a-chip. First, we present the anatomical structures and functional roles of the different skin layers. We then highlight current construction techniques and research findings on skin organoids and skin-on-a-chip. We then discuss in detail the biomedical applications of these emerging technologies. However, current models of skin organoids and skin-on-a-chip still have limitations. Therefore, we summarize the key challenges and explore strategies to improve the complexity and maturation of skin models via the precise control over the microenvironment. In the future, with the advancement of bioengineering technology, skin organoids and skin-on-a-chip will provide more powerful tools for skin disease research and treatment.","PeriodicalId":9553,"journal":{"name":"Burns & Trauma","volume":"24 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-Dimensional Composite Aerogel Scaffolds Based on Electrospun Poly(lactic acid)/Gelatin and Silica-Strontium Oxide Short Fibers Promote Bone Defect Healing","authors":"Jie Cui, Lixiang Zhang, Muhammad Shafiq, Panpan Shang, Xiao Yu, Yangfan Ding, Pengfei Cai, JiaHui Song, Binbin Sun, Mohamed EL-Newehy, Meera Moydeen Abdulhameed, Urszula Stachewicz, Xingping Zhou, Yuan Xu, Xiumei Mo","doi":"10.1093/burnst/tkaf028","DOIUrl":"https://doi.org/10.1093/burnst/tkaf028","url":null,"abstract":"Bone defect regeneration is a dynamic healing process, which relies on intrinsic ability of the body to repair albeit limited healing. The objective of this research was to synthesize hybrid scaffolds based on natural/synthetic polymers and inorganic nanomaterials (NMs). We prepared three-dimensional (3D) composite scaffolds based on flexible silica-strontium oxide (SiO2-SrO) nanofibers and poly(lactic acid)/gelatin (PG) fibers. These scaffolds displayed an ordered porous structure as well as exhibited biocompatibility and biological activity. In vitro release studies demonstrated that the scaffolds enabled sustained and controlled release of silicon ions (Si4+) and strontium ions (Sr2+). Furthermore, these scaffolds not only upregulated the expression of osteogenic-related genes but also promoted tubule-like network formation in human umbilical vein endothelial cells (HUVECs) in vitro. The scaffold enabled concurrent bone regeneration and vascularization in rat skull defect repair. Taken together, our strategy of leveraging the synergistic effect of SiO2-SrO short fibers and PG fibers may have potential to promote bone regeneration and potentially other bio-related disciplines.","PeriodicalId":9553,"journal":{"name":"Burns & Trauma","volume":"15 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143872687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adipose Mesenchymal Stem Cell-derived Exosomes Rescue Mitochondrial Function through SIRT1 to Improve Diabetic Wound Healing","authors":"Xiaozhi Bai, Yu Li, Peng Wang, Zhigang Xu, Jingtao Wei, Ting He, Juntao Han","doi":"10.1093/burnst/tkaf017","DOIUrl":"https://doi.org/10.1093/burnst/tkaf017","url":null,"abstract":"Background Diabetic wounds represent the most common type of chronic wounds. Persistent inflammation and elevated oxidative stress are hallmark features of chronic wounds, where macrophage phenotypic polarization playing a critical role in the healing process. Although adipose-derived mesenchymal stem cell exosomes (ADSC-exos) have shown therapeutic potential for diabetic wounds, their precise mechanisms remain elucidated. Methods A streptozotocin-induced diabetic mouse model and high glucose-stimulated RAW 264.7 macrophages were utilized to mimic diabetic microenvironments. Wound tissues were collected from patients with diabetic foot ulcer. A skin incision model was established in mice and ADSC-exos were given subcutaneously. Streptozotocin-induced diabetic myeloid-specific sirt1−/− mice SIRT1 siRNA-transfected macrophages were employed to investigate the role of SIRT1 in vivo and in vitro. Wound healing rates were quantified. Mitochondrial function, lysosomal activity, autophagy flux, and inflammation status were systematically assessed. Results In diabetic mice and high glucose-treated macrophages, lysosomal dysfunction preceded mitochondrial and autophagy flux impairments. SIRT1 expression was significantly reduced in both diabetic wound tissues and macrophages, accompanied by M1 macrophage polarization. SIRT1 interference experiments revealed that the impact of ADSC-exos on mitochondrial function, autophagy flux, and inflammatory response were partially dependent on SIRT1. Notably, the therapeutic effects of ADSC-exos on mitochondrial and autophagic pathways were markedly attenuated upon SIRT1 suppression. Conclusions These findings demonstrate that ADSC-exos promotes diabetic wound healing by restoring mitochondrial function and autophagy via SIRT1 activation. These findings highlight the therapeutic potential of ADSC-exos and provide a mechanistic foundation for future exosome engineering strategies.","PeriodicalId":9553,"journal":{"name":"Burns & Trauma","volume":"134 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PANoptosis: a new insight into the mechanism of ischemia–reperfusion injury","authors":"Huapei Song, Fengjun Wang","doi":"10.1093/burnst/tkaf026","DOIUrl":"https://doi.org/10.1093/burnst/tkaf026","url":null,"abstract":"Programmed cell death, which occurs via modes such as apoptosis, necroptosis and pyroptosis, is an important mechanism for host defence against pathogens and inflammation-mediated immune responses. Recently, interactions between various types of cell death have gradually been discovered. PANoptosis is a newly discovered mode of programmed cell death that involves apoptosis, necroptosis and pyroptosis and is closely related to many diseases. Ischaemia–reperfusion injury (IRI) is common in patients with blood circulation disorders such as those related to burns, traumatic shock, surgery, organ transplantation and thrombus. However, the literature on the role of PANoptosis in IRI is limited. Herein, we systematically described the emergence of PANoptosis as a cell death mode, clinical evidence of its occurrence, the molecular mechanisms of PANoptosis and its role in IRI. This study is expected to provide novel approaches for the prevention and treatment of tissue and organ IRI after severe burns.","PeriodicalId":9553,"journal":{"name":"Burns & Trauma","volume":"5 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143818962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovations in hydrogel therapies for diabetic wound healing: bridging the gap between pathophysiology and clinical application","authors":"Yao Wang, Haomin Wu, Yan Pan, Yibo Xiao, Yingying Chen, Shuhao Yang, Jun Wang, Wanyue Feng, Cheng Hu, Xiangke Niu, Xin Yong, Jin Yang, Xulin Hu","doi":"10.1093/burnst/tkaf025","DOIUrl":"https://doi.org/10.1093/burnst/tkaf025","url":null,"abstract":"Diabetic wounds (DWs), which are complex and challenging to treat due to delayed healing and incomplete regeneration, pose a significant burden on global healthcare systems. Existing clinical interventions, which mainly comprise debridement, decompression, and wound dressings, have limited efficacy. In addition, DW pathogenesis is complex, with diabetic peripheral neuropathy (DPN), diabetic peripheral arterial disease (PAD), and diabetic foot infections (DWIs) further complicating wound management. Owing to their unique versatility, tunability, and hydrophilicity, hydrogels show promise in several biomedical applications, including DW management. They can effectively promote DW healing by loading therapeutic substances for on-demand release. Given the distinct physiological milieu of DWs, hydrogels with tailored attributes can be engineered to enable on-demand drug release, optimize the wound microenvironment, and cater to the diverse stages of wound healing. Based on the clinical status and pathophysiological features of DWs, this review explores hydrogel wound dressings with the following effects: hypoglycemic, nerve regeneration, vascular regeneration, anti-infective, and bone repair. Additionally, the strategy for applying hydrogels to DWs has been comprehensively studied to provide a robust theoretical foundation for DW treatment and pave the way for clinical translation.","PeriodicalId":9553,"journal":{"name":"Burns & Trauma","volume":"8 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143813592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-Omics Analysis of Small Extracellular Vesicles in Osteoarthritis: Bridging the Gap between Molecular Insights and Clinical Applications","authors":"Tianqi Wang, Chiew Yong Ng, Bryan Zheng Jie Ng, Wei Seong Toh, James Hoi Po Hui","doi":"10.1093/burnst/tkaf023","DOIUrl":"https://doi.org/10.1093/burnst/tkaf023","url":null,"abstract":"Osteoarthritis (OA) is a prevalent degenerative joint disorder with significant socioeconomic impact. Despite advances in understanding its pathophysiology, current therapeutic strategies remain largely palliative. Small extracellular vesicles (sEV) have emerged as crucial mediators of intercellular communication in joint tissues, offering new insights into OA pathogenesis and potential therapeutic targets. This review explores the application of multi-omics approaches to sEV research in OA, assessing how these advanced technologies are contributing to our understanding of the disease and their potential to revolutionize OA management. We discuss the latest findings on the role of sEV in OA, the applications of multi-omics technologies in deciphering sEV cargo, the progress towards clinical translation, and the challenges and opportunities in this field. By synthesizing current knowledge and identifying key research gaps, this review provides a roadmap for leveraging sEV multi-omics to bridge the gap between molecular discoveries and clinical applications in OA.","PeriodicalId":9553,"journal":{"name":"Burns & Trauma","volume":"34 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143666074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}