Burns & Trauma最新文献

{"title":"Exosomes derived from fibroblasts enhance skin wound angiogenesis by regulating HIF-1α/VEGF/VEGFR pathway","authors":"Yunxia Chen, Wenjing Yin, Zhihui Liu, Guang Lu, Xiaorong Zhang, Jiacai Yang, Yong Huang, Xiaohong Hu, Cheng Chen, Ruoyu Shang, Wengang Hu, Jue Wang, Han-Ming Shen, Jun Hu, Gaoxing Luo, Weifeng He","doi":"10.1093/burnst/tkae071","DOIUrl":"https://doi.org/10.1093/burnst/tkae071","url":null,"abstract":"Background Angiogenesis is vital for tissue repair but insufficient in chronic wounds due to paradoxical growth factor overexpression yet reduced neovascularization. Therapeutics physiologically promoting revascularization remain lacking. This study aims to investigate the molecular mechanisms underlying fibroblast-derived exosome-mediated angiogenesis during wound repair. Methods To assess the effects of fibroblasts derived exosomes on wound healing and angiogenesis, a full-thickness mouse skin injury model was established, followed by pharmacological inhibition of exosome secretion. The number and state of blood vessels in wounds were assessed by immunofluorescence, immunohistochemistry, hematoxylin–eosin staining, and laser Doppler imaging system. The high-throughput miRNA sequencing was carried out to detect the miRNA profiles of fibroblast-derived exosomes. The roles of candidate miRNAs, their target genes, and relevant pathways were predicted by bioinformatic online software. The knockdown and overexpression of candidate miRNAs, co-culture system, matrigel assay, pharmacological blockade, cell migration, EdU incorporation assay, and cell apoptosis were employed to investigate their contribution to angiogenesis mediated by fibroblast-derived exosomes. The expression of vascular endothelial growth factor A (VEGFA), vascular endothelial growth factor receptor 2 (VEGFR2), hypoxia-inducible factor 1α (HIF-1α), von Hippel–Lindau (VHL), and proline hydroxylases 2 was detected by western blot, co-immunoprecipitation, immunofluorescence, real-time quantitative polymerase chain reaction, flow cytometry, and immunohistochemistry. Furthermore, a full-thickness mouse skin injury model based on type I diabetes mellitus induced by streptozotocin was established for estimating the effect of fibroblast-derived exosomes on chronic wound healing. Results Pharmacological inhibition of exosome biogenesis markedly reduces neovascularization and delays murine cutaneous wound closure. Topical administration of fibroblast-secreted exosomes rescues these defects. Mechanistically, exosomal microRNA-24-3p suppresses VHL E3 ubiquitin ligase levels in endothelial cells to stabilize hypoxia-inducible factor-1α and heighten vascular endothelial growth factor signaling. MicroRNA-24-3p-deficient exosomes exhibit attenuated pro-angiogenic effects. Strikingly, topical application of exosomes derived from fibroblasts onto chronic wounds in diabetic mice improves neovascularization and healing dynamics. Conclusions Overall, we demonstrate central roles for exosomal miR-24-3p in stimulating endothelial HIF-VEGF signaling by inhibiting VHL-mediated degradation. The findings establish fibroblast-derived exosomes as promising acellular therapeutic candidates to treat vascular insufficiency underlying recalcitrant wounds.","PeriodicalId":9553,"journal":{"name":"Burns & Trauma","volume":"58 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144153363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Primary repair of proximal anterior cruciate ligament injury: a global expert consensus statement","authors":"Shurong Zhang, Tian Xia, Xuesong Dai, Gregory S DiFelice, Lars Engebretsen, Alberto Gobbi, Christiaan H Heusdens, Gordon M MacKay, Bertrand Sonnery-cottet, Jelle P van der List, Shu-Hang P Yung, Jianquan Wang, Yinghui Hua","doi":"10.1093/burnst/tkae079","DOIUrl":"https://doi.org/10.1093/burnst/tkae079","url":null,"abstract":"The application of primary repair for anterior cruciate ligament (ACL) injuries remains controversial, and evidence-based guidelines have not yet been established. Remarkable advancements in arthroscopic techniques and biological stimuli have been achieved in the past decades, which may change expectations regarding the potential of ACL healing and clinical outcomes for patients. In this study, a global expert consensus on the primary repair of proximal ACL injuries was established. A panel of 16 experts from the fields of sports medicine and arthroscopic surgery was invited to participate in the compilation of this consensus statement. This project followed the Delphi approach to the consensus process involving steering, rating, and peer review groups. Ultimately, 14 statements were retained: four achieved unanimous support, six achieved strong consensus, and four did not achieve consensus. The expert consensus statement established in this study focused on surgical indications, decision-making, surgical techniques, adjunctive methods, prognostic factors, and rehabilitation following ACL repair. The accepted recommendations in these areas will assist doctors and therapists in standardizing the management of related pathologies. The consensus statement clearly states that the tear site and tissue quality are important for ensuring successful ACL repair, while other factors should also be considered. ACL reconstruction remains the gold standard for ACL repair until long-term follow-up data demonstrates otherwise.","PeriodicalId":9553,"journal":{"name":"Burns & Trauma","volume":"47 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144130672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indocyanine green and protoporphyrin IX fluorescence imaging of inflammation, hypoxia, and necrosis of burns.","authors":"Aiping Liu,Marien Ochoa,Matthew S Reed,Mary Junak,Joana Pashaj,Brian W Pogue,Angela L F Gibson","doi":"10.1093/burnst/tkaf021","DOIUrl":"https://doi.org/10.1093/burnst/tkaf021","url":null,"abstract":"","PeriodicalId":9553,"journal":{"name":"Burns & Trauma","volume":"31 1","pages":"tkaf021"},"PeriodicalIF":5.3,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144114266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in mesenchymal stem cells and their derivatives for promoting peripheral nerve regeneration","authors":"Shuai Wei, Jin Dong, Qian Hu, Jinyu Bai, Xiang Gao, Huajian Shan, Lei Sheng, Jun Dai, Fengxian Jiang, Lide Tao, Bing Yan, Xiaozhong Zhou","doi":"10.1093/burnst/tkaf027","DOIUrl":"https://doi.org/10.1093/burnst/tkaf027","url":null,"abstract":"Peripheral nerve injury constitutes a complex neurotraumatic pathology characterized by mechanical disruption of neural integrity, manifesting as multimodal sensorimotor deficits and impaired neuromuscular coordination. The primary clinical interventions include surgical tension-free suturing of the severed nerve ends and autologous nerve transplantation. Despite these interventions, patients often experience complications, and the outcomes are not entirely satisfactory for either patients or clinicians. Mesenchymal stem cells (MSCs) have gradually become a novel therapeutic option, with burgeoning preclinical evidence elucidating their multimodal therapeutic potential in peripheral nerve reconstruction. This research has produced promising outcomes, contributing to both fundamental research and translational medicine. However, a comprehensive synthesis of the roles of MSCs and their derivatives in nerve regeneration is still lacking. This article presents a review of the current research advancements in this area, aiming to encourage further investigations and therapeutic applications of MSCs and their derivatives in peripheral nerve injury and regenerative medicine.","PeriodicalId":9553,"journal":{"name":"Burns & Trauma","volume":"25 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144097243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NUFIP1-mediated ribophagy regulates immune function of dendritic cells in polymicrobial sepsis","authors":"Li-yu Zheng, Peng-yi He, Peng-yue Zhao, Yu Duan, Meng-yao Wu, Shu-ting Wei, Yao Wu, Ning Dong, Lei Zheng, Ren-qi Yao, Yong-ming Yao","doi":"10.1093/burnst/tkaf034","DOIUrl":"https://doi.org/10.1093/burnst/tkaf034","url":null,"abstract":"Background Dendritic cells are crucial in the development of sepsis, yet the effect of ribophagy on dendritic cell activation remains unclear. This study aimed to investigate the potential role of nuclear fragile X mental retardation-interacting protein 1 (NUFIP1), a selective autophagy receptor, on sequestering ribosomes in autophagosomes to maintain dendritic cell function during early stages of sepsis. Methods Splenic dendritic cells were isolated using CD11c+microbeads and treated with lipopolysaccharide. Sepsis models were generated using cecal ligation and puncture. Expression of dendritic cell surface molecules was detected using flow cytometry. Cytokine level was quantified using enzyme-linked immunosorbent assay kits. Laser scanning confocal microscopy was employed to observe ribophagy and endoplasmic reticulum (ER) morphology. Transmission electron microscopy was used to examine autophagosomes containing ribosomes. Western blotting was performed to determine the levels of ribophagy- and ER stress-associated proteins. Results The results showed that NUFIP1-mediated ribophagy was significantly activated under septic challenge and facilitated the functional activation of dendritic cells by mitigating excessive ER stress. Deletion of Nufip1resulted in reduced expression of surface molecules on dendritic cells, inhibited T-cell proliferation, exacerbated peripheral immunosuppression and severe multiple organ damage, and increased mortality. Salubrinal, a specific inhibitor of EIF2A dephosphorylation, rescued dendritic cell dysfunction in septic mice with Nufip1deficiency. Mechanistically, NUFIP1 interacted directly with ATF4 and regulated its nuclear translocation. Conclusion These findings suggest that NUFIP1 regulates ER stress through the EIF2AK3–ATF4–DDIT3 pathway, highlighting its critical regulatory role in sepsis. Thus, NUFIP1 represents a new target for sepsis therapy.","PeriodicalId":9553,"journal":{"name":"Burns & Trauma","volume":"18 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144088269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fibroblast Exosomes Promote Wound Healing and Improve the Quality of Healed Skin via miR-29a-3p-Mediated KEAP1/Nrf2 Pathway Activation","authors":"Lingfeng Yan, Dejiang Fan, Jiacai Yang, Jue Wang, Xiaohong Hu, Xiaorong Zhang, Yong Huang, Hong Wang, Wenjing Yin, Xin Cai, Ruoyu Shang, Canhua Huang, Gaoxing Luo, Weifeng He","doi":"10.1093/burnst/tkaf035","DOIUrl":"https://doi.org/10.1093/burnst/tkaf035","url":null,"abstract":"Background Wound healing is a sophisticated biological process characterized by the orchestrated interplay of diverse cellular components, growth factors, and signaling cascades. Recent research has highlighted the pivotal role of fibroblast exosomes in mediating intercellular communication and facilitating tissue regeneration. This investigation aimed to elucidate the therapeutic efficacy of fibroblast exosomes in enhancing wound repair mechanisms, with a particular emphasis on their differential effects in normal and diabetic wound healing paradigms. Methods A mouse full-thickness skin defect model was used to evaluate the effects of fibroblast exosomes on wound re-epithelialization, granulation tissue formation, and epidermal barrier function. Molecular and cellular experiments were conducted to analyze the roles of exosomes in epidermal stem cell proliferation, migration, differentiation, and antioxidant stress, with further validation of the associated signaling pathways. The therapeutic efficacy was additionally confirmed in a type 1 diabetic mouse model. Results Fibroblast exosomes significantly enhanced wound re-epithelialization by promoting the proliferation, migration, and differentiation of epidermal stem cells. Additionally, exosomes increased fibroblast abundance and myofibroblast activation, facilitating granulation tissue formation as well as improving extracellular matrix (ECM) deposition and the biomechanical properties of healed skin. Furthermore, exosomes improved epidermal barrier function by upregulating tight junction proteins (e.g., Claudin-1 and ZO-1) and reducing transepidermal water loss (TEWL). In diabetic mouse models, exosomes accelerated wound closure, restored ECM deposition and biomechanical integrity, and repaired epidermal barrier function. Mechanistically, exosomes target the 3′ untranslated region (UTR) of Keap1 mRNA through miR-29a-3p and activate the KEAP1/Nrf2 antioxidant pathway, mitigating oxidative stress and protecting epidermal stem cells from reactive oxygen species (ROS)-induced damage. Conclusion Fibroblast exosomes alleviate oxidative damage by modulating the KEAP1/Nrf2 pathway through miR-29a-3p and enhancing epidermal stem cell function. These exosomes exhibit remarkable therapeutic potential in accelerating wound healing and improving healing quality under both normal and diabetic conditions, offering a robust foundation for innovative therapeutic strategies.","PeriodicalId":9553,"journal":{"name":"Burns & Trauma","volume":"1 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144083180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring machine learning strategies for single-cell transcriptomic analysis in wound healing","authors":"Jianzhou Cui, Mei Wang, Chenshi Lin, Xu Xu, Zhenqing Zhang","doi":"10.1093/burnst/tkaf032","DOIUrl":"https://doi.org/10.1093/burnst/tkaf032","url":null,"abstract":"Wound healing is a highly orchestrated, multi-phase process that involves various cell types and molecular pathways. Recent advances in single-cell transcriptomics and machine learning have provided unprecedented insights into the complexity of this process, enabling the identification of novel cellular subpopulations and molecular mechanisms underlyingtissue repair. In particular, single-cell RNA sequencing (scRNA-seq) has revealedsignificant cellular heterogeneity, especially withinfibroblast populations, and has provided valuable information on immune cell dynamics during healing. Machine learning algorithms have enhanced data analysis by improving cell clustering, dimensionality reduction, and trajectory inference, leading to a better understanding of wound healing at the single-cell level. This review synthesizes the latest findings on the application of scRNA-seq and machine learning in wound healing research, with a focus on fibroblast diversity, immune responses, and spatial organization of cells. The integration of these technologies has the potential to revolutionize therapeutic strategies for chronic wounds, fibrosis, and tissue regeneration, offering new opportunities for precision medicine. By combining computational approacheswith biological insights, this review highlights the transformative impact of scRNA-seq and machine learning on wound healing research.","PeriodicalId":9553,"journal":{"name":"Burns & Trauma","volume":"231 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143946102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular vesicles and their mimetics: clinical application prospects in medical aesthetics","authors":"Zixuan Sun, Jiali Li, Zhijing Wu, Jiaxin Zhang, Tangrong Wang, Yuzhou Zheng, XueZhong Xu, Wei Ding, Hui Qian, Yulin Tan","doi":"10.1093/burnst/tkaf033","DOIUrl":"https://doi.org/10.1093/burnst/tkaf033","url":null,"abstract":"The persistent desire for beauty has fueled the rapid development of medical aesthetics. Multiple approaches, including cosmetics, drugs, and cell therapies, have been developed to improve skin conditions. However, none of these methods achieves the intended outcomes and may produce adverse repercussions. Consequently, it is crucial to develop effective and safe therapies to promote skin repair and regeneration. Extracellular vesicles (EVs) are cell-derived, lipid bilayer-encapsulated nanoparticles containing multiple active components. There is now strong evidence that EV therapies can improve skin barrier function, reduce scarring, inhibit aging and pigmentation, and suppress hair loss, highlighting the potential for cosmeceutical development based on EVs. This review summarizes the latest applications of natural EVs, preconditioned and engineered EVs, and EV mimetics in medical aesthetics. Additionally, the article discusses advanced delivery strategies for EVs and the commercial prospects of EVs and provides insights into present security concerns, regulations, and the field’s future directions.","PeriodicalId":9553,"journal":{"name":"Burns & Trauma","volume":"16 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144083183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrospun Nanofiber Scaffolds Loaded with Copper Oxide for Repairing Traumatic Brain Injury through Restoring Copper Homeostasis and Regulating Pyroptosis pathway","authors":"Yumei An, Sunao Li, Xinqi Huang, Xueshi Chen, Mingyuan Xu, Chen Chen, Xuefeng Zhou, Haiyan Shan, Luyang Tao, Mingyang Zhang","doi":"10.1093/burnst/tkaf030","DOIUrl":"https://doi.org/10.1093/burnst/tkaf030","url":null,"abstract":"Background TBI is one of the leading causes of injury and disability worldwide. Pyroptosis, a specific type of programmed cell death (PCD) triggered by inflammatory signals, plays a significant part in the pathological process after TBI. Copper ions play an important role in anti-inflammation and anti-oxidative stress. There is a more active copper metabolism in neurons after injury, and that neurons may require more copper ions and downstream copper-based enzymes to maintain normal physiological functions. Methods We developed an electrostatic spinning scaffold loaded with copper oxide (CuO@ PCL/gelatin) to achieve small-dose local administration and avoid toxic side effects. The membranes underwent preparation and characterization through various techniques including Fourier transform infrared spectroscopy, measurement of water contact angle, antibacterial experiment, scanning electron microscopy, and assessment of in vitro release of copper. In addition, we used a controlled cortical impact (CCI) to establish a TBI model in mice to examine the effect of CuO@PG on TBI-induced pyroptosis and the ability of the membranes to heal brain injury. Results CuO@PG inhibited TBI-induced neuronal pyroptosis. CuO@PG can inhibit the expression of the pyroptosis-related proteins. Moreover, CuO@PG also alleviates brain edema and the degree of neurodegeneration in the acute phase of TBI. The neuroprotective effect of CuO@PG was further confirmed by wire-grip test, open field test, Morris water maze test. Lastly, the beneficial results were significantly inhibited by the use of the copper chelator TTM. Conclusions In this study, we successfully constructed electrostatically spun scaffolds loaded with copper oxide to achieve slow, continuous and low-dose copper supply to the local brain, which provides a new theoretical basis for the imbalance of copper homeostasis in the brain after TBI.","PeriodicalId":9553,"journal":{"name":"Burns & Trauma","volume":"3 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organoids/organs-on-chips towards biomimetic human artificial skin","authors":"Yuting Huang, Xiaoyan Wu, Yongxin Xu, Nengjie Yang, Peipei Xi, Yunan Wang, Yujuan Zhu, Xiaodong Chen","doi":"10.1093/burnst/tkaf029","DOIUrl":"https://doi.org/10.1093/burnst/tkaf029","url":null,"abstract":"As the largest organ in the human body, the skin protects the body from pathogens and harmful substances through physical, chemical and immune barrier functions. However, accurately replicating the complex physiology of human skin in mouse models remains a significant challenge. Accurately replicating the complex physiology of human skin in mouse models remains a significant challenge, making the development of bionic artificial skin particularly important. In recent years, skin organoid and skin-on-a-chip technologies have greatly enhanced in vitro skin modeling, overcoming many limitations of traditional approaches. In this review, we comprehensively summarize important advances in research on skin organoids and skin-on-a-chip. First, we present the anatomical structures and functional roles of the different skin layers. We then highlight current construction techniques and research findings on skin organoids and skin-on-a-chip. We then discuss in detail the biomedical applications of these emerging technologies. However, current models of skin organoids and skin-on-a-chip still have limitations. Therefore, we summarize the key challenges and explore strategies to improve the complexity and maturation of skin models via the precise control over the microenvironment. In the future, with the advancement of bioengineering technology, skin organoids and skin-on-a-chip will provide more powerful tools for skin disease research and treatment.","PeriodicalId":9553,"journal":{"name":"Burns & Trauma","volume":"24 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}