Biochemia medica最新文献

Cardiac biomarkers in term newborns with common pathological conditions during the first 24 hours postpartum. 产后24小时内具有常见病理状况的足月新生儿的心脏生物标志物。

IF 1.8

Biochemia medica Pub Date : 2025-10-15 Epub Date: 2025-08-15 DOI: 10.11613/BM.2025.030702

Helena Karlović, Marjana Jerković Raguž, Ivanka Mikulić, Vinka Mikulić, Vajdana Tomić

{"title":"Cardiac biomarkers in term newborns with common pathological conditions during the first 24 hours postpartum.","authors":"Helena Karlović, Marjana Jerković Raguž, Ivanka Mikulić, Vinka Mikulić, Vajdana Tomić","doi":"10.11613/BM.2025.030702","DOIUrl":"10.11613/BM.2025.030702","url":null,"abstract":"Introduction: Cardiac biomarkers may help diagnose and monitor different neonatal conditions, but their concentrations are still underexplored in common pathologies diagnosed within the first day. This study compared N-terminal pro b-type natriuretic peptide (NT-proBNP), high sensitivity troponin I (hs-TnI), creatine kinase (CK), and its isoenzyme creatine kinase-myocardial band (CK-MB) concentrations and activities, measured within the first 24 hours (h) postpartum, between the healthy term neonates and those with jaundice, perinatal infection, transient neurological abnormalities (TNA), and heart ultrasound abnormalities.Materials and methods: The study included 100 term newborns, whose cardiac biomarkers' concentrations were determined from the serum within 24 h postpartum on the Alinity ci analyzer (Abbott, Chicago, USA). The Mann-Whitney and Kruskal-Wallis tests, performed in SPSS Statistics v. 25.0 (IBM Corp., Armonk, USA), were used to test the significance of differences between the study groups, with P < 0.05 indicating significance.Results: Within first 24 h postpartum healthy newborns had significantly higher CK activities compared to those with jaundice (P = 0.047), perinatal infection (P = 0.012), or combination of both (P = 0.017). Lower CK activities were demonstrated in perinatal infection compared to TNA (P = 0.041). Other biomarkers' concentrations did not differ between the study groups. No significant differences were found in cardiac biomarkers' concentrations regarding gender or heart ultrasound findings.Conclusions: During the first 24 h postpartum, only CK activities differed between healthy newborns and those with the common pathologic conditions, being lower in the newborns with jaundice and/or infection. Analogous differences were present between newborns with infection and those with TNA.","PeriodicalId":94370,"journal":{"name":"Biochemia medica","volume":"35 3","pages":"030702"},"PeriodicalIF":1.8,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12334941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144877697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

What is uric acid concentration in urine in patients with uric acid kidney stones? - a case study. 尿酸性肾结石患者尿中尿酸浓度是多少？-案例研究。

IF 1.8

Biochemia medica Pub Date : 2025-10-15 Epub Date: 2025-08-15 DOI: 10.11613/BM.2025.031001

Tomáš Šálek, Pavel Musil, Irena Zlatníková

引用次数: 0

Diagnostic value of leucine-rich alpha-2-glycoprotein 1 and calprotectin in acute appendicitis: a short review. 富亮氨酸-2-糖蛋白1和钙保护蛋白在急性阑尾炎中的诊断价值

IF 1.8

Biochemia medica Pub Date : 2025-10-15 DOI: 10.11613/BM.2025.030504

Lea Gvozdanović, Željka Dragila, Luka Maršić, Denis Klapan, Željka Dujmić, Josip Samardžić, Zrinka Mihaljević, Višnja Nesek-Adam

{"title":"Diagnostic value of leucine-rich alpha-2-glycoprotein 1 and calprotectin in acute appendicitis: a short review.","authors":"Lea Gvozdanović, Željka Dragila, Luka Maršić, Denis Klapan, Željka Dujmić, Josip Samardžić, Zrinka Mihaljević, Višnja Nesek-Adam","doi":"10.11613/BM.2025.030504","DOIUrl":"10.11613/BM.2025.030504","url":null,"abstract":"Acute abdominal pain accounts for 7-10% of all emergency department visits. Appendicitis, being one of the leading causes of acute abdominal surgery, poses significant diagnostic challenges. Negative appendectomy rates can be as high as 40%, while complications occur in more than 90% if the diagnosis is missed during the initial examination. Therefore, more effective preoperative screening is required for patients with suspected appendicitis. Recent studies suggest that novel biomarkers, particularly leucine-rich alpha 2-glycoprotein and calprotectin, may improve the early and accurate diagnosis of acute appendicitis by demonstrating high specificity and sensitivity, respectively. Unlike C-reactive protein, the production of leucine-rich alpha 2-glycoprotein 1 and calprotectin takes place at the site of inflammation. As a result, their raised concentrations might be evident early in a disease, possibly before other common markers of inflammation start to rise. This literature review aims to assess the potential role of leucine-rich alpha 2-glycoprotein 1 and calprotectin as diagnostic biomarkers in patients with suspected acute appendicitis, acknowledging the need for additional data to fully assess their diagnostic accuracy.","PeriodicalId":94370,"journal":{"name":"Biochemia medica","volume":"35 3","pages":"030504"},"PeriodicalIF":1.8,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12523658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145310511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analyzing clinical laboratory data outcomes in retrospective cohort studies using TriNetX. 使用TriNetX分析回顾性队列研究的临床实验室数据结果。

IF 1.8

Biochemia medica Pub Date : 2025-10-15 Epub Date: 2025-08-15 DOI: 10.11613/BM.2025.030502

Joshua Wang, Kuo-Wang Tsai, Chien-Lin Lu, Kuo-Cheng Lu

引用次数: 0

Association of inflammatory markers with depression and anxiety in female patients with primary Sjögren's syndrome. 炎症标志物与女性原发性Sjögren综合征患者抑郁和焦虑的关系

IF 1.8

Biochemia medica Pub Date : 2025-10-15 Epub Date: 2025-08-15 DOI: 10.11613/BM.2025.030701

Fanika Mrsić, Ines Vukasović, Andrea Tešija Kuna, Blaženka Ladika Davidović, Jasenka Markeljević

{"title":"Association of inflammatory markers with depression and anxiety in female patients with primary Sjögren's syndrome.","authors":"Fanika Mrsić, Ines Vukasović, Andrea Tešija Kuna, Blaženka Ladika Davidović, Jasenka Markeljević","doi":"10.11613/BM.2025.030701","DOIUrl":"10.11613/BM.2025.030701","url":null,"abstract":"Introduction: Primary Sjögren's syndrome (pSS) is a chronic autoimmune disease affecting exocrine glands and is frequently accompanied by depression and anxiety. Proinflammatory cytokines, particularly interleukin 6 (IL-6), have been implicated in the pathogenesis of both pSS and mood disorders. This study aimed to assess the association between inflammatory markers, disease activity, and psychological symptoms in patients with pSS.Materials and methods: A cross-sectional study was conducted on 60 female patients diagnosed with pSS at Sestre milosrdnice University Hospital Center between 2019 and 2021. Depression and anxiety were evaluated using the Hospital Anxiety and Depression Scale. Inflammatory biomarkers (erythrocyte sedimentation rate, rheumatoid factor, ferritin, fibrinogen, CRP, C3, C4, IL-6) and disease activity indices (ESSDAI, ESSPRI) were analyzed. Statistical analyses, including logistic regression, were applied to determine independent predictors of depression and anxiety.Results: Depression was detected in 39/60 of patients, while 34/60 exhibited anxiety symptoms. Patients with either depression or anxiety had significantly higher IL-6 concentration (P < 0.001 and P = 0.002, respectively). Logistic regression identified IL-6 as an independent predictor of depression (OR = 3.23, 95%CI: 1.07 - 9.80, P = 0.038), while ESSPRI fatigue was a significant predictor of anxiety (OR = 2.01, 95%CI: 1.13 - 3.58, P = 0.018).Conclusions: The findings suggest that IL-6 could be a predictor of pSS-related depression, potentially serving as a biomarker for this extraglandular manifestation and ESSPRI fatigue as a predictor for anxiety.","PeriodicalId":94370,"journal":{"name":"Biochemia medica","volume":"35 3","pages":"030701"},"PeriodicalIF":1.8,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12334945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144877696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perspective and consideration in the application of personalized reference intervals based on biological variation: a four-month observation of a woman with SARS-CoV-2 reinfection. 基于生物学变异的个性化参考区间应用的视角与思考：1例女性SARS-CoV-2再感染的4个月观察

IF 1.8

Biochemia medica Pub Date : 2025-10-15 Epub Date: 2025-08-15 DOI: 10.11613/BM.2025.030901

Gaofeng Hu, Lei Xu, Kai Guo, Chenbin Li

{"title":"Perspective and consideration in the application of personalized reference intervals based on biological variation: a four-month observation of a woman with SARS-CoV-2 reinfection.","authors":"Gaofeng Hu, Lei Xu, Kai Guo, Chenbin Li","doi":"10.11613/BM.2025.030901","DOIUrl":"10.11613/BM.2025.030901","url":null,"abstract":"This study aimed to investigate potential benefit of personalized reference intervals (prRIs) by conducting a four-month observation of a woman with SARS-CoV-2 reinfection. Two types of prRIs were calculated: one derived from the population biological variation (BV) data provided by the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) biological variation database (prRIs_pop.), and the other derived from individual variation data (prRIs_ind.). These were subsequently compared. A total of 110 test results encompassing complete blood count (CBC) and leukocyte differential counts from the case were assessed according to the limits of prRIs_pop., reference change values (RCVs_pop.) and the population-based reference intervals (popRIs). In instances where limited historical health data are available (N ≤ 3), the application of prRIs_pop. was recommended over prRIs_ind. The prRIs_pop. and RCVs_pop. identified a greater number of potential clinical pathological change compared to popRIs (the ratio of potential abnormal values to total test values: prRIs_pop. 22/110, RCVs_pop. 25/110, popRIs 2/110, respectively). The findings suggest that the use of prRIs can be advantageous in clinical settings and is worthy of broader adoption. However, it is essential to choose an appropriate calculation method tailored to the specific clinical context.","PeriodicalId":94370,"journal":{"name":"Biochemia medica","volume":"35 3","pages":"030901"},"PeriodicalIF":1.8,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12334944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144877699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

National recommendations of the Croatian society of medical biochemistry and laboratory medicine: Thyroid function tests from the laboratory point of view. 克罗地亚医学生物化学和实验室医学学会的国家建议：从实验室的角度进行甲状腺功能检查。

IF 1.8

Biochemia medica Pub Date : 2025-10-15 DOI: 10.11613/BM.2025.030505

Adriana Bokulić, Ivana Zec, Domagoj Marijančević, Marija Siter Kuprešanin, Sanja Goreta, Anamarija Đuras, Koraljka Đurić, Sanda Jelisavac Ćosić, Iva Lukić, Tihana Serdar Hiršl, Lada Stanišić, Daniela Šupe-Domić, Alenka Pezo, Marija Kocijančić

{"title":"National recommendations of the Croatian society of medical biochemistry and laboratory medicine: Thyroid function tests from the laboratory point of view.","authors":"Adriana Bokulić, Ivana Zec, Domagoj Marijančević, Marija Siter Kuprešanin, Sanja Goreta, Anamarija Đuras, Koraljka Đurić, Sanda Jelisavac Ćosić, Iva Lukić, Tihana Serdar Hiršl, Lada Stanišić, Daniela Šupe-Domić, Alenka Pezo, Marija Kocijančić","doi":"10.11613/BM.2025.030505","DOIUrl":"10.11613/BM.2025.030505","url":null,"abstract":"Thyroid function tests (TFTs) - thyroid stimulating hormone (TSH), total triiodothyronine (T3), total thyroxine (T4), free triiodothyronine (FT3), free thyroxine (FT4), thyroid peroxidase antibodies (anti-TPO), thyroglobulin antibodies (anti-Tg), TSH receptors antibodies (anti-TSHR), and thyroglobulin (Tg) - are used to diagnose thyroid disorders and are crucial biomarkers for monitoring and managing thyroid cancer. The 2022 national survey results revealed that thyroid function testing is not standardized among Croatian medical-biochemistry laboratories. Laboratories follow individual protocols at each testing stage, from patient preparation to result reporting. To address this, the Working group for laboratory endocrinology of the Croatian society of medical biochemistry and laboratory medicine has developed recommendations based on the latest national and international guidelines, research and the authors' expert opinion. The document aims to standardize all phases of thyroid function testing, with 7 preanalytical, 12 analytical, and 8 postanalytical recommendations, each supported by expert explanations. While primarily directed at Croatian laboratory professionals, this document is also relevant to other healthcare professionals managing thyroid-related health issues.","PeriodicalId":94370,"journal":{"name":"Biochemia medica","volume":"35 3","pages":"030505"},"PeriodicalIF":1.8,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12523660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145310438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum hepcidin concentration is lower in advanced stages of sporadic colorectal cancer. 晚期散发性结直肠癌患者血清hepcidin浓度较低。

IF 1.8

Biochemia medica Pub Date : 2025-10-15 DOI: 10.11613/BM.2025.030703

Tara Rolić, Sanja Mandić, Mazyar Yazdani, Marina Ferenac Kiš, Sonia Distante, Ines Banjari

{"title":"Serum hepcidin concentration is lower in advanced stages of sporadic colorectal cancer.","authors":"Tara Rolić, Sanja Mandić, Mazyar Yazdani, Marina Ferenac Kiš, Sonia Distante, Ines Banjari","doi":"10.11613/BM.2025.030703","DOIUrl":"10.11613/BM.2025.030703","url":null,"abstract":"Introduction: Hepcidin (Hep), a key regulatory hormone of iron (Fe) homeostasis, governs its absorption and storage, and is influenced by inflammation and Fe status. This study investigated serum Hep concentrations and their associations with Fe markers and inflammation in patients with sporadic colorectal cancer (CRC).Materials and methods: We compared serum concentrations of Hep, Fe, unsaturated and total iron binding capacity, transferrin, transferrin saturation, ferritin, C-reactive protein (CRP), interleukin-6 (IL-6) and tumor markers in 82 CRC patients and 58 controls. Statistically significant differences were tested using the Mann-Whitney U test and Student's t test. Additionally, Hep were analyzed according to tumor stage. Colorectal cancer was confirmed histopathologically after colonoscopy with biopsy (TNM staging).Results: Colorectal cancer patients exhibited significantly lower Hep concentrations than controls (8.1 vs. 19.7 ng/mL, P = 0.020). Ferritin was also lower in CRC (109 vs. 250 µg/L, P = 0.002). Hepcidin showed the strongest positive correlation with ferritin in CRC. Inflammatory markers (CRP and IL-6) correlated moderately to weakly with hepcidin in both groups (controls: rho = 0.52 (P < 0.001); CRC: rho = 0.26 (P = 0.022) for CRP and CRC: rho = 0.30 (P = 0.033) for IL-6). Notably, Hep concentrations were lower in patients with advanced tumor stage (T0 vs. T3, P = 0.043).Conclusion: These findings suggest that CRC is associated with lower hepcidin and ferritin concentrations, potentially reflecting complex and cancer-specific dysregulation in Fe metabolism beyond inflammation alone.","PeriodicalId":94370,"journal":{"name":"Biochemia medica","volume":"35 3","pages":"030703"},"PeriodicalIF":1.8,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12523599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145310503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of LDL cholesterol and Lp(a) on monocytes and macrophages in atherosclerosis. LDL胆固醇和Lp(a)对动脉粥样硬化中单核细胞和巨噬细胞的影响。

IF 1.8

Biochemia medica Pub Date : 2025-10-15 DOI: 10.11613/BM.2025.030503

Sabina Ugovšek, Jernej Jeras, Miran Šebeštjen, Janja Zupan

{"title":"Influence of LDL cholesterol and Lp(a) on monocytes and macrophages in atherosclerosis.","authors":"Sabina Ugovšek, Jernej Jeras, Miran Šebeštjen, Janja Zupan","doi":"10.11613/BM.2025.030503","DOIUrl":"10.11613/BM.2025.030503","url":null,"abstract":"Atherosclerosis is an active interaction between lipoproteins and inflammatory cells. Monocytes and macrophages are the most important immune cells involved in the process of atherosclerosis. They interact with atherogenic lipoproteins, in particular low density lipoprotein (LDL) cholesterol and lipoprotein(a) (Lp(a)). The increased concentration of the LDL cholesterol and Lp(a) accelerates the polarization of monocytes and macrophages toward proinflammatory phenotype and the formation of the foam cells. These cells then release large quantities of inflammatory cytokines that stimulate the oxidation of atherogenic lipoproteins that are even more atherogenic and contribute to the formation of foam cells and the secretion of the pro-inflammatory cytokines, thus creating a vicious circle. Surface marker C-C chemokine receptor type 2, expressed on monocytes/macrophages, enables their adhesion and migration into the subendothelial layer. The rupture of the atherosclerotic plaque on one hand, and the ability of the oxidized LDL cholesterol and Lp(a) to trigger arterial thrombosis by different mechanisms on the other hand, result in acute cardiovascular event. Here, we summarize the role of the monocytes and macrophages in atherosclerosis and explore the influence of LDL cholesterol and Lp(a) on monocytes and macrophages during the entire process of atherosclerosis, from its initiation to progression.","PeriodicalId":94370,"journal":{"name":"Biochemia medica","volume":"35 3","pages":"030503"},"PeriodicalIF":1.8,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12523661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145310574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cystatin C for gentamicin dosing - a case study. 庆大霉素剂量的胱抑素C -一个案例研究。

IF 1.8

Biochemia medica Pub Date : 2025-10-15 Epub Date: 2025-08-15 DOI: 10.11613/BM.2025.030902

Tomáš Šálek, Josef Klhůfek, Martin Vodička, Marek Pšenčík

引用次数: 0