Cumulative IgG reagent carryover detected in carryover evaluation of urinary total protein testing using AU5800 biochemistry analyzer.

Abstract

Introduction: Accurate measurement of urinary total protein (UTP) is crucial for diagnosing renal and systemic diseases. This study aims to comprehensively evaluate potential carryover scenarios in UTP testing using AU5800 biochemistry analyzer and to identify factors influencing assay accuracy.

Materials and methods: High-concentration quality control materials and pure water was used to evaluate specific sample probe carryover. Additionally, 24-hour mixed urine samples from patients were used to evaluate specific carryover from the reagent probe, mixbars, and cuvettes. For cumulative sample carryover evaluation, pure water was used as reagent and mixed serum as sample for continuous testing. During the process, 24-hour urine samples were interspersed, and UTP concentrations were measured at 0, 10, 20, and 30 minutes. Cumulative reagent carryover was evaluated by testing pure water sequentially with eight reagents sharing the same analytical unit and inner cuvettes as UTP, with UTP concentrations of 24-hour urine samples recorded at 0, 10, 20, and 30 minutes.

Results: Specific carryover from the sample probe, reagent probe, mixbars, and cuvettes was not detected during carryover evaluation of UTP testing. However, a significant cumulative reagent carryover of Immunoglobulin G (IgG) reagent was observed, while no cumulative sample carryover was identified.

Conclusions: The full range of possible carryover scenarios in UTP testing was evaluated using AU5800 biochemistry analyzer. Our data provide valuable references for evaluating carryover in laboratories, ensuring the accuracy and reliability of laboratory results.