Access microbiologyPub Date : 2025-03-11eCollection Date: 2025-01-01DOI: 10.1099/acmi.0.000995.v3
Melissa M Lacey, Michael J Dillon, Sean Goodman, Victoria Easton, Alison I Graham
{"title":"Towards an inclusive conference experience: evaluation of the Education and Outreach Symposium at the Microbiology Society Annual Conference 2024.","authors":"Melissa M Lacey, Michael J Dillon, Sean Goodman, Victoria Easton, Alison I Graham","doi":"10.1099/acmi.0.000995.v3","DOIUrl":"https://doi.org/10.1099/acmi.0.000995.v3","url":null,"abstract":"<p><p>The Microbiology Society Education and Outreach Symposium serves as a platform for microbiology educators to share contemporary practices with an international audience. The Symposium is held yearly during the Microbiology Society's Annual Conference and has become increasingly popular among conference attendees. In an effort to create an inclusive and engaging environment, the 2024 Symposium included contributions from participants at all career stages and from diverse global regions through a variety of presentation formats, including invited talks, offered presentations, flash talks and posters. Cabaret-style seating was used to encourage discussion amongst participants, and digital tools were used for anonymous feedback and questions after each talk to ensure all voices had an opportunity to be heard. Here, we present an analysis of qualitative and quantitative participant responses addressing two key research questions: (1) Did the Symposium foster an inclusive atmosphere for participants across all career stages? and (2) Was the content engaging and relevant to the audience? A post-Symposium questionnaire revealed strong positive feedback, with all 18 respondents agreeing or strongly agreeing that the 2024 Symposium was both an inclusive environment and covered interesting topics. Thematic content analysis of free-text responses emphasized a high appreciation for the Symposium's diversity in speakers and topics, an inclusive room layout and an overall welcoming feeling. Feedback from participants, along with the authors' own reflections, will actively feed into planning for the 2025 Symposium.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12013379/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144061289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2025-03-11eCollection Date: 2025-01-01DOI: 10.1099/acmi.0.000947.v3
Melissa M Lacey, Michael J Dillon, Sean Goodman, Victoria Easton, Alison I Graham
{"title":"Towards an inclusive conference experience: evaluation of the Education and Outreach Symposium at the Microbiology Society Annual Conference 2024.","authors":"Melissa M Lacey, Michael J Dillon, Sean Goodman, Victoria Easton, Alison I Graham","doi":"10.1099/acmi.0.000947.v3","DOIUrl":"10.1099/acmi.0.000947.v3","url":null,"abstract":"<p><p>The Microbiology Society Education and Outreach Symposium serves as a platform for microbiology educators to share contemporary practices with an international audience. The Symposium is held yearly during the Microbiology Society's Annual Conference and has become increasingly popular among conference attendees. In an effort to create an inclusive and engaging environment, the 2024 Symposium included contributions from participants at all career stages and from diverse global regions through a variety of presentation formats, including invited talks, offered presentations, flash talks and posters. Cabaret-style seating was used to encourage discussion amongst participants, and digital tools were used for anonymous feedback and questions after each talk to ensure all voices had an opportunity to be heard. Here, we present an analysis of qualitative and quantitative participant responses addressing two key research questions: (1) Did the Symposium foster an inclusive atmosphere for participants across all career stages? and (2) Was the content engaging and relevant to the audience? A post-Symposium questionnaire revealed strong positive feedback, with all 18 respondents agreeing or strongly agreeing that the 2024 Symposium was both an inclusive environment and covered interesting topics. Thematic content analysis of free-text responses emphasized a high appreciation for the Symposium's diversity in speakers and topics, an inclusive room layout and an overall welcoming feeling. Feedback from participants, along with the authors' own reflections, will actively feed into planning for the 2025 Symposium.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143627324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2025-03-07eCollection Date: 2025-01-01DOI: 10.1099/acmi.0.000901.v3
Calvin Ka-Fung Lo, Lauren Hughes, Cole Schonhofer, William R Bowie, Iain McCormick, Andrew Kirker
{"title":"Isolated Neisseria meningitidis-associated endophthalmitis in an immunocompetent host: case report and literature review.","authors":"Calvin Ka-Fung Lo, Lauren Hughes, Cole Schonhofer, William R Bowie, Iain McCormick, Andrew Kirker","doi":"10.1099/acmi.0.000901.v3","DOIUrl":"10.1099/acmi.0.000901.v3","url":null,"abstract":"<p><p><b>Introduction.</b> <i>Neisseria meningitidis</i> is a Gram-negative diplococcus with significant infectious sequelae, including meningitis and disseminated meningococcal bloodstream infection. Rarely has it been reported in the context of endophthalmitis without central nervous system involvement. We report a clinical case of isolated meningococcal endophthalmitis in an immunocompetent patient and present a literature review on published cases, including treatment regimens and clinical outcomes. <b>Case Presentation.</b> A 51-year-old male with no significant medical history presented to the emergency department with acute vision loss in the right eye after returning from Mexico. Ophthalmic examination was consistent with endophthalmitis, presumed to be endogenous in the absence of recent ocular trauma or surgery. Vitreous culture was positive for growth of Gram-negative diplococci, subsequently identified as <i>N. meningitidis</i>. Blood and cerebrospinal fluid cultures were negative for growth of similar or implicative pathogens. There was no evidence of disseminated meningococcal infection; imaging did not demonstrate any drainable collections or sequelae of extension into the central nervous system. The patient was treated with intravitreal antibiotics as well as topical steroids and antibiotics. In addition, he completed a 2-week course of systemic antibiotics. Visual outcome was unfortunately poor. <b>Conclusion.</b> This case illustrates a rare case of isolated endophthalmitis secondary to meningococcus, presumably from a nasopharyngeal source. In these clinical scenarios, clinicians should perform a thorough evaluation for predisposing immunodeficiencies.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936350/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2025-03-07eCollection Date: 2025-01-01DOI: 10.1099/acmi.0.000865.v3
Jessica M Labonté, Kathryn L Campbell, Jordan R Walker, Milena A Rodriguez-Pilco
{"title":"Ubiquitous microbial contaminants associated with scientific ocean drilling.","authors":"Jessica M Labonté, Kathryn L Campbell, Jordan R Walker, Milena A Rodriguez-Pilco","doi":"10.1099/acmi.0.000865.v3","DOIUrl":"10.1099/acmi.0.000865.v3","url":null,"abstract":"<p><p>Deep-sea subsurface samples typically have low microbial biomass, making them more susceptible to contamination. Potential contaminants can be introduced during any step of the scientific process, including drilling (contamination from the drilling fluid and lubricants and seawater); sample preparation (contamination from air and human handling); and DNA extraction, amplification and sequencing (contamination from reagents). The International Ocean Discovery Program (IODP) samples that are dedicated to microbiological analyses (known as MBIO samples) are routinely tested for contamination by injecting known concentrations of fluorescent microspheres or tracers directly into the drilling fluids. These tracers are a great tool to determine the level of drilling fluid contamination on board, but they are not consistently used, and contamination of the samples could occur during other steps of sample processing and analysis. Moreover, there is an increased risk of contamination dominating the results of microbial surveys using PCR amplification of marker genes. Here, we built a database of common contaminants through the screening of contamination controls from available 16S rRNA gene amplicon datasets from past IODP expeditions. These controls included various lubricants used on board, drilling fluids, seawater, DNA extraction blanks and PCR blanks. The order <i>Burkholderiales</i> dominated most of the very low biomass samples, including negative controls, indicating the order's ubiquity and its potential to be overamplified with common 16S rRNA amplification protocols. We amplified the 16S rRNA gene from preserved IODP legacy microbiological core samples and tested their level of contamination using the database. We also looked at published studies that did not sequence negative controls. Our results demonstrate that the type of drilling, amount of manipulation of the sample prior to preservation and sample depth, often associated with biomass, can influence the level of contamination within subsurface samples. This work provides an analysis framework for microbial taxonomic survey studies from low biomass subsurface samples for future scientific ocean drilling expeditions.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936376/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2025-02-26eCollection Date: 2025-01-01DOI: 10.1099/acmi.0.000897.v3
Sophie Gregg, Niamh Purcell, Maeve Doyle, Grace Chan
{"title":"Reducing blood culture contamination: an environmental imperative.","authors":"Sophie Gregg, Niamh Purcell, Maeve Doyle, Grace Chan","doi":"10.1099/acmi.0.000897.v3","DOIUrl":"10.1099/acmi.0.000897.v3","url":null,"abstract":"<p><p>Blood culture (BC) investigation remains the gold standard for the diagnosis of bloodstream infections. However, BC contamination can have clinical implications for the patient, cost implications for service providers and less well-documented, environmental impacts. Efforts to reduce BC contamination are a long-standing theme in quality improvement initiatives in emergency departments (EDs) and hospitals, prompted by hospital costs, healthcare inefficiencies and antimicrobial stewardship efforts. The WHO's global analysis of healthcare waste in the context of COVID-19 has reported that tens of thousands of tonnes of extra medical waste were produced from the response to the COVID-19 pandemic, basing its estimates on the quantity of personal protective equipment. Additionally, recent literature has also shown increased BC contamination rates during the COVID-19 pandemic. We performed a retrospective review of the trend of BC contamination during the COVID-19 pandemic in our institution's ED. We further discuss some of the potential implications of BC contamination, including potential environmental, economic and efficiency implications.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11865496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143525761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2025-02-21eCollection Date: 2025-01-01DOI: 10.1099/acmi.0.000760.v4
Linda M Maya-Rodríguez, Gabriela Gómez-Verduzco, Francisco J Trigo-Tavera, Leticia Moreno-Fierros, Verónica Rojas-Trejo, Rosa Elena Miranda-Morales
{"title":"A comparative in silico analysis of the vlhA gene regions of Mycoplasma gallisepticum and Mycoplasma synoviae isolates from commercial hen farms in Mexico.","authors":"Linda M Maya-Rodríguez, Gabriela Gómez-Verduzco, Francisco J Trigo-Tavera, Leticia Moreno-Fierros, Verónica Rojas-Trejo, Rosa Elena Miranda-Morales","doi":"10.1099/acmi.0.000760.v4","DOIUrl":"10.1099/acmi.0.000760.v4","url":null,"abstract":"<p><p>Avian mycoplasmosis, caused by <i>Mycoplasma synoviae</i> and <i>Mycoplasma gallisepticum</i>, poses significant economic challenges due to respiratory issues, reduced egg production and soft eggshells. The variable lipoprotein haemagglutinin (VlhA) protein, crucial for pathogenicity, comprises conserved (MSPB) and variable (MSPA) regions. The aim of this study was to identify the conserved region of <i>vlhA</i> gene sequences in field strain. We examined <i>vlhA</i> sequences from field strains collected in central Mexico (Jalisco and Mexico City). Specifically, we analysed 124 deformed eggs and 10 laying hens from 9 farms with Hy-line and Bovans breeds. Using PCR targeting the <i>mgc2</i> and 16S rRNA genes, we characterized 24 field strains, 4 of which were <i>Myc. synoviae</i> and 20 of which were <i>Myc. gallisepticum</i>. We analysed the <i>vlhA</i> regions, based on the AF035624.1 reference sequence, with American Type Culture Collection strains as positive controls. Additionally, we validated the PCR with 20 negative samples from <i>Mycoplasma</i> isolation without the need for cultivation. We identified two amplification regions: MSPB and MSPA. Bioanalysis revealed relationships between our field samples and avian <i>Mycoplasma</i> sequences in GenBank, alongside similarities with lipoproteins present in <i>Acholeplasma laidlawii</i> PG8 and <i>Escherichia coli</i>. Given the significance of the VlhA protein in pathogenicity and immune evasion, the identified conserved sequences hold potential as therapeutic targets and for phylogenetic studies.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11845793/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143485301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2025-02-19eCollection Date: 2025-01-01DOI: 10.1099/acmi.0.000962.v3
Beatrice Achan, Tonny Luggya, Robert Innocent Ebwongu, Simon Sekyanzi, Henry Kajumbula
{"title":"Tossing the coin of extended-spectrum β-lactamase: prevalence of extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolated from patients with sepsis.","authors":"Beatrice Achan, Tonny Luggya, Robert Innocent Ebwongu, Simon Sekyanzi, Henry Kajumbula","doi":"10.1099/acmi.0.000962.v3","DOIUrl":"10.1099/acmi.0.000962.v3","url":null,"abstract":"<p><p><b>Background.</b> <i>Klebsiella pneumoniae</i> is part of the ESKAPE (<i>Enterococcus faecium</i>, <i>Staphylococcus aureus</i>, <i>K. pneumoniae</i>, <i>Acinetobacter baumannii</i>, <i>Pseudomonas aeruginosa</i> and <i>Enterobacter</i> spp.) group of multidrug-resistant (MDR) pathogens. <i>K. pneumoniae</i> is the leading cause of antimicrobial resistance-associated mortality and the second leading cause of nosocomial bloodstream infections (BSIs), globally and in sub-Saharan Africa. Therefore, it was aimed to determine the antibiotic resistance patterns of <i>K. pneumoniae</i> isolated from blood cultures of patients with features of sepsis at Mulago National Referral Hospital, Uganda. <b>Methods.</b> The cross-sectional study on patients with features of sepsis utilized <i>K. pneumoniae</i> (<i>n</i>=30) isolated from positive blood culture specimens. The antibiotic resistance profile was determined by the Clinical and Laboratory Standards Institute's Kirby-Bauer disc diffusion method, which was used to classify the isolates as susceptible, intermediate and resistant. <i>K. pneumoniae</i> isolates that were resistant to third-generation cephalosporins were subjected to extended-spectrum <i>β</i>-lactamase (ESBL) screening and confirmation using the double-disc synergy test using cefotaxime, ceftazidime, ceftriaxone, cefotaxime-clavulanic acid and ceftazidime-clavulanic acid. The results were analysed for frequencies. <b>Results.</b> <i>K. pneumoniae</i> isolates showed emerging resistance to imipenem at 13% (4 out of 30) followed by amikacin at 17% (5 out of 30). There was intermediate resistance to gentamycin at 60% (18 out of 30). However, <i>K. pneumoniae</i> showed the highest resistance to piperacillin at 100% (30 out of 30) followed by sulphamethoxazole-trimethoprim and cefepime, both showing a percentage of 97% (29 out of 30). Up to 16 out of 30 (53.3%) of <i>K. pneumoniae</i> were positive for ESBL production, whilst 14 out of 30 (46.7%) were negative. <b>Conclusion.</b> There was a high prevalence of antibiotic-resistant <i>ESBL</i>-producing <i>K. pneumoniae</i> isolates from BSI of patients with features of sepsis in Uganda's Mulago National Referral Hospital.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11840160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143470331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2025-02-17eCollection Date: 2025-01-01DOI: 10.1099/acmi.0.000925.v3
Hai Trong Nguyen, Ravendra Garg, Andrea Kroeker, Volker Gerdts, Darryl Falzarano, Qiang Liu
{"title":"Immunogenicity of virus-like particle vaccine candidates against SARS-CoV-2 infection.","authors":"Hai Trong Nguyen, Ravendra Garg, Andrea Kroeker, Volker Gerdts, Darryl Falzarano, Qiang Liu","doi":"10.1099/acmi.0.000925.v3","DOIUrl":"10.1099/acmi.0.000925.v3","url":null,"abstract":"<p><p>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to evolve, potentially leading to variants of concern that could become more transmissible, resist treatment, evade host immunity and reduce the effectiveness of currently available vaccines. Improved vaccines are still required as vaccination remains the most effective strategy against this virus. We have produced two SARS-CoV-2 virus-like particles (VLPs) using a baculovirus BacMam expression platform and examined their immunogenicity in mice. VLP1 contains the spike protein from the Wuhan strain, whereas VLP2 contains that of an Omicron variant. Mice immunized with VLP1 and boosted with VLP2 developed significantly higher antibodies in the sera, as well as higher numbers of IFN-γ secreting cells than the control group. Furthermore, both VLPs induced virus-neutralizing antibodies against Wuhan and Omicron variants. In conclusion, VLPs have the potential for the development of a safe and effective vaccine against SARS-CoV-2 variants.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11833050/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143451404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2025-02-17eCollection Date: 2025-01-01DOI: 10.1099/acmi.0.000732.v3
Michael K Porter, Alexander Viloria Winnett, Linhui Hao, Natasha Shelby, Jessica A Reyes, Noah W Schlenker, Anne E Romano, Colton Tognazzini, Matthew Feaster, Ying-Ying Goh, Michael Gale, Rustem F Ismagilov
{"title":"The ratio between SARS-CoV-2 RNA viral load and culturable viral titre differs depending on the stage of infection: a case study of household transmission in an adult male.","authors":"Michael K Porter, Alexander Viloria Winnett, Linhui Hao, Natasha Shelby, Jessica A Reyes, Noah W Schlenker, Anne E Romano, Colton Tognazzini, Matthew Feaster, Ying-Ying Goh, Michael Gale, Rustem F Ismagilov","doi":"10.1099/acmi.0.000732.v3","DOIUrl":"10.1099/acmi.0.000732.v3","url":null,"abstract":"<p><p>Effective public health measures for communicable diseases rely on the ability to identify infectious individuals and prevent transmission from those individuals. For severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the presence of replication-competent virus in specimens from an individual is the gold standard for confirming infectiousness. However, viral culture from clinical specimens is difficult and infrequently performed. Instead, infectiousness may be inferred based on the abundance of viral RNA (or viral load) in a specimen, which is more easily assessed. For this reason, understanding the relationship between RNA viral load and infectious viral titre has important implications for public health strategy. In this case report, we quantified incident, longitudinal SARS-CoV-2 viral loads collected from saliva and nasal-swab specimens, and viral titre from nasal-swab specimens. We observed that the relationship between viral load and viral titre decreases by over five orders of magnitude throughout the course of the infection. Our work demonstrates the potential for infectious virus even in specimens with low viral loads collected during the early phases of infection.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11833051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143451405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Access microbiologyPub Date : 2025-02-14eCollection Date: 2025-01-01DOI: 10.1099/acmi.0.000958.v3
Jack W Stone, John T Munnoch, Paul A Hoskisson
{"title":"Whole-genome sequencing of the Streptomyces coelicolor bldA39 mutant (J1700) reveals hundreds of previously unknown mutations.","authors":"Jack W Stone, John T Munnoch, Paul A Hoskisson","doi":"10.1099/acmi.0.000958.v3","DOIUrl":"10.1099/acmi.0.000958.v3","url":null,"abstract":"<p><p>We report the genome sequence of the <i>bldA39</i> (J1700) mutant of <i>Streptomyces coelicolor</i>, a historically important strain that is deficient in sporulation and antimicrobial production. The <i>S. coelicolor</i> J1700 strain was used extensively from the 1980s onwards to underpin important discoveries in development and antibiotic production in <i>Streptomyces</i>. The <i>bldA</i> gene encodes a leucyl tRNA, required for the translation of the rare TTA codon found in ~2% of genes in <i>Streptomyces</i>. The whole genome of <i>S. coelicolor</i> J1700 was obtained via Illumina sequencing and mapped to the <i>S. coelicolor</i> M145 reference genome. Analysis of the genome sequence compared to <i>S. coelicolor</i> M145 identified the known <i>bldA39</i> mutation (T>C) and revealed more than 300 further mutations, likely associated with the <i>S. coelicolor</i> J1501 genetic background the strain was created in, including the nature of the <i>hisA1</i> and <i>uraA1</i> alleles used extensively in genetic mapping experiments and several mutations in natural product biosynthetic gene clusters. This work highlights the importance of whole-genome sequencing of historically important strains.</p>","PeriodicalId":94366,"journal":{"name":"Access microbiology","volume":"7 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11836424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143461548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}