Journal of pharmacy & bioallied sciences最新文献

{"title":"Salivary Biomarkers vs. Conventional Biopsy for Oral Cancer Detection in India: A Comparative Study.","authors":"Anuj Singh Parihar, Mehak Tariq, Abhishek K Singh, Priyesh Mathur, Akanksha Bhattacharjee, Kriti Priyadarshini","doi":"10.4103/jpbs.jpbs_152_25","DOIUrl":"10.4103/jpbs.jpbs_152_25","url":null,"abstract":"<p><strong>Objective: </strong>Oral cancer, predominantly oral squamous cell carcinoma (OSCC), constitutes a major global health concern with alarmingly high-morbidity and mortality rates. Early detection is crucial for improving patient outcomes. This study aims to compare the diagnostic accuracy of salivary biomarkers against conventional biopsy in detecting oral cancer.</p><p><strong>Methods: </strong>This prospective study recruited 120 participants with suspected oral lesions from the People's Dental Academy, Bhopal, India. Unstimulated saliva samples were collected and analyzed using enzyme-linked immunosorbent assay (ELISA) for a panel of four biomarkers: interleukin-8 (IL-8), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-<i>α</i>), and matrix metalloproteinase-9 (MMP-9). The adults in the study also had a biopsy performed with a scalpel which is the standard way of doing it and served as a gold standard in the diagnosis process. Both salivary biomarkers and biopsy received sensitivity, specificity, positive predictive value, and negative predictive value calculations.</p><p><strong>Results: </strong>Salivary analysis revealed significantly elevated levels of all four biomarkers in patients with OSCC compared to those with benign lesions. The combined biomarker panel demonstrated a sensitivity of 88%, specificity of 80%, PPV of 76%, and NPV of 91% in detecting OSCC. In comparison, scalpel biopsy exhibited a sensitivity of 96%, specificity of 100%, PPV of 100%, and NPV of 98%.</p><p><strong>Conclusion: </strong>While scalpel biopsy remains the gold standard for OSCC diagnosis, our findings suggest that salivary biomarkers, particularly when used in combination, offer a promising adjunctive tool for oral cancer detection. This non-invasive approach may prove valuable in identifying high-risk individuals who require further investigation with biopsy, potentially leading to earlier diagnosis and improved patient outcomes.</p>","PeriodicalId":94339,"journal":{"name":"Journal of pharmacy & bioallied sciences","volume":"17 Suppl 1","pages":"S278-S280"},"PeriodicalIF":0.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12156604/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144287995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smile Enhancement by Innovative Approach of Direct Composite Veneering.","authors":"Reetu Arora, Munish Singla, Harleen Kaur, Saloni Watts, Vaani Mahajan","doi":"10.4103/jpbs.jpbs_1904_24","DOIUrl":"10.4103/jpbs.jpbs_1904_24","url":null,"abstract":"<p><p>This article reports two cases of innovative direct composite veneering for smile enhancement. The technique was used for aesthetic concerns like fluorosis, interdental spacing, and discoloration, providing a minimally invasive yet highly effective approach for the patients. Both cases were carried out by using a polyvinyl chloride (PVC) template to achieve precise placement of the composite material to produce natural and aesthetically pleasing results. The conservative nature of the technique, combined with meticulous execution, provided significant aesthetic improvements, meeting patient expectations. This method demonstrates the efficacy of direct composite veneering in achieving superior results while preserving tooth structure, reinforcing its value as a reliable option in aesthetic dentistry.</p>","PeriodicalId":94339,"journal":{"name":"Journal of pharmacy & bioallied sciences","volume":"17 Suppl 1","pages":"S1050-S1052"},"PeriodicalIF":0.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12156692/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144287999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study Protocol: Association of Platelet-Rich Plasma (PRP) with Endometrium Thickness and Pregnancy Outcome in Women with Recurrent Implantation Failure (RIF).","authors":"Aru Chaudhary, Akash More, Namrata Choudhary","doi":"10.4103/jpbs.jpbs_315_25","DOIUrl":"10.4103/jpbs.jpbs_315_25","url":null,"abstract":"<p><strong>Background: </strong>This study, conducted in the Wardha district, Recurrent implantation failure (RIF) and thin endometrium are significant challenges in IVF success. Various techniques have been explored to enhance endometrial receptivity. Platelet-rich Plasma (PRP) is an emerging technique that can enhance the endometrium lining and clinical pregnancy rate. This study will demonstrate the effectiveness of the infusion of the Platelet-rich Plasma (PRP) intra utrine for increasing the endometrium lining and compare the success rate in clinical pregnancy outcome in patients after infusing PRP and not infusing PRP undergoing frozen-thawed embryo transfer.</p><p><strong>Objective: </strong>The objective of the study will be to investigate the role of PRP in addressing recurrent implantation failure due to thin endometrium and to assess the success rate of clinical pregnancy outcome in frozen-thawed embryo transfer after inducing PRP in the patient with RIF.</p><p><strong>Methodology: </strong>A total of 190 patients will be selected for the study. The experimental population will be divided into two groups, i.e., 95 patients will be in the Study group, which will be exposed to PRP infusion, and 95 patients will be in the Control group, which will be exposed to the placebo. The study will be conducted on the population with thin endometrium and recurrent implantation failure (RIF) and females below 50 years of age. In this study, the control group and the study group will be compared, and the result of the intervention given to the study group. This study will also demonstrate how PRP is prepared and then injected in the patient.</p><p><strong>Expected result: </strong>Therefore, the study will demonstrate that PRP improves the endometrium thickness, reduces the recurrent implantation failure, and also increases the clinical pregnancy rate in the patients who are infused with the PRP. It will demonstrate that PRP is very cost-effective as it is an affordable and low-risk adjunct to improve endometrium receptivity.</p>","PeriodicalId":94339,"journal":{"name":"Journal of pharmacy & bioallied sciences","volume":"17 Suppl 1","pages":"S928-S931"},"PeriodicalIF":0.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12156690/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study Protocol: Evaluating the Impact of Diabetic Medications on Semen Quality: A Comparative Analysis of Diabetic Patients and Nondiabetic Controls with Correlation to Fertility Potential.","authors":"Lakhan P Padwal, Akash More, Namrata Choudhary, Ganesh L Kalasakar","doi":"10.4103/jpbs.jpbs_316_25","DOIUrl":"10.4103/jpbs.jpbs_316_25","url":null,"abstract":"<p><strong>Background: </strong>Infertility is a growing global concern, with diabetes emerging as a significant contributor to male reproductive dysfunction. Diabetes negatively affects sperm quality by altering hormonal balance, increasing oxidative stress, and impairing sperm motility, morphology, and count. While previous studies have established a link between diabetes and infertility, limited research has focused on the effects of diabetic medications on sperm parameters and fertility outcomes.</p><p><strong>Objectives: </strong>This study aims to evaluate the impact of diabetic medications on semen quality by comparing diabetic patients receiving pharmacological treatment, untreated diabetic patients, and nondiabetic controls. The research will focus on assessing sperm count, motility, morphology, and oxidative stress markers to determine how different diabetes management strategies influence male fertility.</p><p><strong>Methodology: </strong>A comparative observational study will be conducted over 6 months at Wardha Test Tube Baby Centre, AVBRH, Sawangi Meghe, Wardha. Male participants diagnosed with diabetes and experiencing infertility will be categorized into four groups: (1) Type 1 diabetes patients on insulin therapy, (2) Type 2 diabetes patients on oral medications, (3) diabetic patients without pharmacological treatment, and (4) nondiabetic controls. A semen analysis will be performed using standard laboratory techniques to evaluate sperm parameters and oxidative stress levels. ANOVA and correlation tests will be used for statistical analysis.</p><p><strong>Expected results: </strong>This study aims to provide detailed insights into how different diabetes treatments impact sperm vitality, helping identify the best options for fertility preservation. By generating comparative data on the effects of insulin and oral medications on sperm quality and fertility, the findings will guide clinicians in recommending the most suitable treatment for diabetic men concerned about reproduction. Additionally, understanding how sperm quality changes over time in diabetic men will help determine critical periods for intervention based on disease duration and treatment type. A comparison between treated diabetic patients and nondiabetic controls will further clarify the effects of diabetic medications on sperm health.</p><p><strong>Study implications: </strong>This study will help evaluate how different diabetes treatments, such as insulin and oral medications, affect sperm health. Understanding these effects can lead to personalized treatment approaches that reduce the negative impact on male fertility. Additionally, the research will provide valuable insights into the complex relationship between diabetes, its management, and male reproductive health. This knowledge will help healthcare professionals develop better strategies for managing infertility in diabetic men, ultimately improving outcomes for couples struggling with conception.</p>","PeriodicalId":94339,"journal":{"name":"Journal of pharmacy & bioallied sciences","volume":"17 Suppl 1","pages":"S932-S935"},"PeriodicalIF":0.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12156715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study to Establish the Relationship between Rabeprazole (PPI) and Acute Interstitial Nephritis (AIN).","authors":"Bite Bapurao, Swanand Pathak, Ranjit Sidram Ambad","doi":"10.4103/jpbs.jpbs_1965_24","DOIUrl":"10.4103/jpbs.jpbs_1965_24","url":null,"abstract":"<p><strong>Introduction: </strong>Proton pump inhibitors (PPIs) are the most commonly used drug worldwide. PPIs are benzimidazole derivatives and are basic in nature; therefore, they are dispensed in enteric-coated tablets or capsules to avoid degradation from gastric acid. When PPIs are administered by oral route of administration, they get absorbed from small intestine and cross the acidic parietal cell canaliculus. In this acidic environment, PPI is activated and produces a sulfenamide form of the drug. This activated form binds with the H⁺ K⁺ ATPase enzyme covalently, which results in irreversible inhibition of acid secretion.</p><p><strong>Aim: </strong>Study to establish the relationship between rabeprazole (PPI) and acute interstitial nephritis (AIN).</p><p><strong>Objective: </strong>To find the effect of PPI alone, PPI with diclofenac, PPI with ofloxacin on renal function tests.</p><p><strong>Methods: </strong>In the present study, we are analyzing PPI (omeprazole) as a main group. The group is again divided into three subgroups containing six rats in each. The relation between PPI and AIN is observed by the administration of PPI alone. To find this relation, we combine the PPI with nonsteroidal anti-inflammatory drug (NSAID) diclofenac and antimicrobial agent ofloxacin.</p><p><strong>Results: </strong>Rabeprazole 20 mg/kg/day alone showed a statistically significant difference in serum creatinine and blood urea values. Rabeprazole 20 mg/kg/day and ofloxacin 60 mg/kg/day did not show a statistically significant difference in serum creatinine and blood urea values. Rabeprazole 20 mg/kg/day and diclofenac showed a statistically significant difference in serum creatinine and blood urea values.</p><p><strong>Conclusion: </strong>With longer administration of PPIs, it did produce derangement of renal function tests (RFTs), and with concurrent administration of drugs like ofloxacin and diclofenac, it hastened the process of renal damage as indicated by deranged RFTs.</p>","PeriodicalId":94339,"journal":{"name":"Journal of pharmacy & bioallied sciences","volume":"17 Suppl 1","pages":"S378-S380"},"PeriodicalIF":0.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12156607/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Comparative Study of Platelet Rich Plasma (PRP) and Plasma Rich in Growth factor (PRGF) in Endometrial Thickness, Implantation Rate and Pregnancy Outcomes in IVF Patients.","authors":"Mariyam Khan, Akash More, Namrata Anjankar","doi":"10.4103/jpbs.jpbs_409_25","DOIUrl":"10.4103/jpbs.jpbs_409_25","url":null,"abstract":"<p><strong>Background: </strong>Platelet-rich plasma (PRP) and Plasma Rich in Growth Factors (PRGF) are promising regenerative therapies aimed at enhancing endometrial thickness and improving <i>in vitro</i> fertilization (IVF) outcomes. This study aims to assess the impact of PRP and PRGF on endometrial development, implantation success, and pregnancy rates in IVF patients. A total of 10 ml of venous blood will be drawn from participants and processed to isolate platelets. PRP will be prepared through centrifugation to concentrate platelets, while PRGF will be generated using a leukocyte-poor method and activated with calcium chloride. Endometrial thickness (EMT) will be measured via transvaginal ultrasound (TVS) on the fifth day of the menstrual cycle. If EMT is below 7 mm, PRP or PRGF will be administered, with additional treatments as needed. The study will compare endometrial response, implantation rates, clinical pregnancy rates, and live birth rates between the PRP and PRGF groups. Additionally, safety and potential side effects will be closely monitored to ensure patient well-being.</p><p><strong>Objectives: </strong>The study will assess the impact of PRP and PRGF on endometrial thickness and compare pregnancy outcomes, including clinical pregnancy rates in patients treated with either PRP or PRGF. The study will also evaluate the safety of these treatments and identify any adverse effects associated with them.</p><p><strong>Materials and methods: </strong>On the 8^th day of the menstrual cycle, transvaginal ultrasound (TVS) will assess endometrial thickness. If it's less than 7 mm, PRP will be injected into the uterine cavity using a PRP catheter. EMT will be reassessed after 48 hours, with additional PRP if needed. Embryo transfer will occur only when EMT reaches 7 mm, using the GnRH antagonist protocol. If EMT exceeds 7 mm during ovarian stimulation, no PRP will be given. After one week of estrogen administration, 1 ml of PRGF will be injected under ultrasound guidance. Three PRGF insertions will constitute one cycle, repeated based on endometrial thickness.</p><p><strong>Expected result: </strong>Our study demonstrates that PRGF is more effective in increasing endometrium thickness than PRP in patients with thin endometrium.</p>","PeriodicalId":94339,"journal":{"name":"Journal of pharmacy & bioallied sciences","volume":"17 Suppl 1","pages":"S952-S955"},"PeriodicalIF":0.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12156504/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Focused Nurse-Midwife Interventions on Postnatal Discomfort and Quality of Life Among Postnatal Mothers - A Quasi Experimental Study.","authors":"Baby Kannan, Theranirajan Ethiraj, Shankar Shanmugam Rajendran, Valveeman Vijayalakshmi, Seethalakshmi Durairaj, Sabari Raja","doi":"10.4103/jpbs.jpbs_1878_24","DOIUrl":"10.4103/jpbs.jpbs_1878_24","url":null,"abstract":"<p><strong>Background: </strong>It has been shown that postnatal discomfort is one of the common issues that postpartum women go through and, in most cases, have a low quality of life, thus impairing their recovery and overall well-being. This research study was aimed at establishing the effectiveness of nurse-midwife-specific interventions aimed at reducing postnatal discomfort and enhancing the quality of life among postpartum women.</p><p><strong>Methods: </strong>This quasi-experimental study had a control and an intervention group of postpartum women. Standardized questionnaires to assess pain and quality of life data were collected. Statistical analysis by Chi-square test and <i>t</i>-test to compare scores before and after the intervention in both the groups were performed.</p><p><strong>Results: </strong>Intervention outcomes were a significant reduction in pain scores and quality-of-life improvements compared to the control group. Therefore, interventions targeted by the nurse-midwife may improve postpartum recovery.</p><p><strong>Conclusion: </strong>Focused interventions by the nurse-midwife improved outcomes related to postnatal recovery. The treatment can be useful for inclusion in postpartum care to enhance the quality of life and maternal well-being.</p>","PeriodicalId":94339,"journal":{"name":"Journal of pharmacy & bioallied sciences","volume":"17 Suppl 1","pages":"S652-S654"},"PeriodicalIF":0.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12156539/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Detoxifying Effects of <i>Spirulina</i> in Promoting Liver Health and Heavy Metal Elimination - A Review.","authors":"Sunil Namdeo Thitame, Ashwini Ashok Aher, Viddyasagar Mopagar","doi":"10.4103/jpbs.jpbs_570_25","DOIUrl":"https://doi.org/10.4103/jpbs.jpbs_570_25","url":null,"abstract":"<p><p><i>Spirulina</i>, a blue-green algae rich in essential nutrients, has garnered significant attention for its potential health benefits, particularly its role in liver detoxification and the elimination of heavy metals. This review explores the detoxifying effects of <i>spirulina</i>, focusing on its ability to enhance liver health and facilitate the removal of toxic metals from the body. <i>Spirulina</i> contains bioactive compounds such as phycocyanin, chlorophyll, and various antioxidants, which have been shown to possess hepatoprotective properties. Studies highlight <i>spirulina's</i> capacity to reduce oxidative stress, inflammation, and liver enzyme markers associated with liver damage. Furthermore, <i>spirulina's</i> chelating agents support the binding and excretion of heavy metals, such as lead, mercury, and cadmium, from the body. The synergistic effects of <i>spirulina</i> on liver function and detoxification processes present a promising avenue for alternative therapeutic interventions in mitigating the harmful effects of environmental pollutants and supporting liver health. This review synthesizes current research findings, providing an in-depth understanding of the molecular mechanisms through which <i>spirulina</i> may aid in detoxification and liver protection, while also highlighting the need for further clinical studies to validate these therapeutic claims.</p>","PeriodicalId":94339,"journal":{"name":"Journal of pharmacy & bioallied sciences","volume":"17 Suppl 1","pages":"S43-S45"},"PeriodicalIF":0.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12156544/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In Vitro</i> Analysis of Compressive Strength of Three Different Aesthetic Restorative Materials.","authors":"Rohit Nagar, Simone Grandini, Carlo Gaeta, Sumit Dubey, Niladri Maiti, Madhura Avinash Jadhav, Neerieza Konthoujam","doi":"10.4103/jpbs.jpbs_1884_24","DOIUrl":"10.4103/jpbs.jpbs_1884_24","url":null,"abstract":"<p><strong>Aim: </strong>The study aims to assess and contrast the compressive strengths of three aesthetically pleasing restorative materials.</p><p><strong>Materials and methods: </strong>Using metallic molds, 30 pellets were created, then split into 3 groups of 10, each meeting the required specifications. Glass Ionomer Cement (GIC) cylindrical specimens are in Group A; Resin Modified GIC (RMGIC) cylindrical specimens are in Group B; and Zirconomer cylindrical specimens are in Group C. A universal testing equipment was used to compress all of the samples, and the results were acquired. Tukey's <i>post hoc</i> test and one-way ANOVA were used for statistical analysis.</p><p><strong>Result: </strong>Zirconomer's compressive strength is significantly higher than that of RMGIC and GIC.</p><p><strong>Conclusion: </strong>The compressive strength of Zirconomer is the highest. As a result, Zirconomer may be the preferred material for aesthetic restorative procedures.</p>","PeriodicalId":94339,"journal":{"name":"Journal of pharmacy & bioallied sciences","volume":"17 Suppl 1","pages":"S391-S393"},"PeriodicalIF":0.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12156493/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144287711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In Vitro</i> Analysis of Healing Properties of Platelet-Rich Fibrin (PRF) vs. Collagen Membranes in Guided Tissue Regeneration.","authors":"B Saravana Kumar, Ushma H Prajapati, Neelam Das, K Vel Latha, Vishal S Shah, Arshiya F Mohammadi","doi":"10.4103/jpbs.jpbs_491_25","DOIUrl":"10.4103/jpbs.jpbs_491_25","url":null,"abstract":"<p><strong>Aim: </strong>The present study sought to assess, using growth factor release and tissue integration, the <i>in vitro</i> healing characteristics of collagen membranes and platelet-rich fibrin (PRF) in guided tissue regeneration (GTR).</p><p><strong>Materials and methods: </strong>The control was commercially available collagen membranes; PRF membranes were produced using a normal centrifugation technique. Scanning electron microscopy (SEM) was used to examine structural features. Key growth factors-including transforming growth factor-beta 1 (TGF-β1), platelet-derived growth factor (PDGF-BB), and vascular endothelial growth factor (VEGF)-were assessed using enzyme-linked immunosorbent assays (ELISA). Using scratch tests, we assessed wound healing capacity and fibroblast migration. The student's <i>t</i>-test was used for statistical analysis; <i>P</i> < 0.001 was considered to be significant.</p><p><strong>Results: </strong>While collagen membranes showed a consistent porosity shape, PRF showed a rich fibrin network, including embedded platelets and leukocytes. At 14 days, PRF showed much more cell growth (<i>P</i> < 0.001). Growth factor analysis showed that PRF generated more TGF-β1, PDGF-BB, and VEGF with consistent release (<i>P</i> < 0.001). PRF shows greater healing properties than collagen membranes with enhanced cellular proliferation, movement, and release of growth factors.</p><p><strong>Conclusion: </strong>These results suggest that PRF might be a more effective biomaterial for use in guided tissue regeneration.</p>","PeriodicalId":94339,"journal":{"name":"Journal of pharmacy & bioallied sciences","volume":"17 Suppl 1","pages":"S519-S521"},"PeriodicalIF":0.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12156619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144287712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}