Plant signaling & behavior最新文献_第4页

Quasi-targeted metabolomics revealed isoliquiritigenin and lauric acid associated with resistance to tobacco black shank. 准靶向代谢组学揭示了与烟草黑柄病抗性相关的isisiquiritigenin和月桂酸。

Plant signaling & behavior Pub Date : 2024-12-31 Epub Date: 2024-03-25 DOI: 10.1080/15592324.2024.2332019

Shiwen Peng, Fangling Shu, Yanhui Lu, Dongsheng Fan, Dehong Zheng, Gaoqing Yuan

{"title":"Quasi-targeted metabolomics revealed isoliquiritigenin and lauric acid associated with resistance to tobacco black shank.","authors":"Shiwen Peng, Fangling Shu, Yanhui Lu, Dongsheng Fan, Dehong Zheng, Gaoqing Yuan","doi":"10.1080/15592324.2024.2332019","DOIUrl":"10.1080/15592324.2024.2332019","url":null,"abstract":"Tobacco black shank (TBS), caused by Phytophthora nicotianae, is a severe disease. Plant root exudates play a crucial role in mediating plant-pathogen interactions in the rhizosphere. However, the specific interaction between key secondary metabolites present in root exudates and the mechanisms of disease resistance remains poorly understood. This study conducted a comprehensive comparison via quasi-targeted metabolomic analysis on the root exudate metabolites from the tobacco cultivar Yunyan87 and K326, both before and after inoculation with P. nicotianae. The results showed that the root exudate metabolites changed after P. nicotianae inoculation, and the root exudate metabolites of different tobacco cultivar was significantly different. Furthermore, homovanillic acid, lauric acid, and isoliquiritigenin were identified as potential key compounds for TBS resistance based on their impact on the mycelium growth of the pathogens. The pot experiment showed that isoliquiritigenin reduced the incidence by 55.2%, while lauric acid reduced it by 45.8%. This suggests that isoliquiritigenin and lauric acid have potential applications in the management of TBS. In summary, this study revealed the possible resistance mechanisms of differential metabolites in resistance of commercial tobacco cultivar, and for the first time discovered the inhibitory effects of isoliquiritigenin and homovanillic acid on P. nictianae, and attempt to use plants secondary metabolites of for plant protection.","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"19 1","pages":"2332019"},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10965109/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140290091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The 14-3-3 protein nt GF14e interacts with CIPK2 and increases low potassium stress in tobacco. 14-3-3 蛋白 nt GF14e 与 CIPK2 相互作用，增加烟草的低钾胁迫。

Plant signaling & behavior Pub Date : 2024-12-31 Epub Date: 2024-06-02 DOI: 10.1080/15592324.2024.2359257

Li Xu, Yifei Lu, Jiarui Jiang, Qian Chen, Yong Xu, Qili Mi, Haiying Xiang, Liming Lu, Xuemei Li, Qian Gao, Liqin Li

{"title":"The 14-3-3 protein nt GF14e interacts with CIPK2 and increases low potassium stress in tobacco.","authors":"Li Xu, Yifei Lu, Jiarui Jiang, Qian Chen, Yong Xu, Qili Mi, Haiying Xiang, Liming Lu, Xuemei Li, Qian Gao, Liqin Li","doi":"10.1080/15592324.2024.2359257","DOIUrl":"10.1080/15592324.2024.2359257","url":null,"abstract":"Potassium (K+) plays a role in enzyme activation, membrane transport, and osmotic regulation processes. An increase in potassium content can significantly improve the elasticity and combustibility of tobacco and reduce the content of harmful substances. Here, we report that the expression analysis of Nt GF14e, a 14-3-3 gene, increased markedly after low-potassium treatment (LK). Then, chlorophyll content, POD activity and potassium content, were significantly increased in overexpression of Nt GF14e transgenic tobacco lines compared with those in the wild type plants. The net K+ efflux rates were severely lower in the transgenic plants than in the wild type under LK stress. Furthermore, transcriptome analysis identified 5708 upregulated genes and 2787 downregulated genes between Nt GF14e overexpressing transgenic tobacco plants. The expression levels of some potassium-related genes were increased, such as CBL-interacting protein kinase 2 (CIPK2), Nt CIPK23, Nt CIPK25, H+-ATPase isoform 2 a (AHA2a), Nt AHA4a, Stelar K+ outward rectifier 1(SKOR1), and high affinity K+ transporter 5 (HAK5). The result of yeast two-hybrid and luciferase complementation imaging experiments suggested Nt GF14e could interact with CIPK2. Overall, these findings indicate that NtGF14e plays a vital roles in improving tobacco LK tolerance and enhancing potassium nutrition signaling pathways in tobacco plants.","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"19 1","pages":"2359257"},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11152103/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141201339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of mitogen-activated protein kinases substrates in Arabidopsis using kinase client assay. 利用激酶客户测定法鉴定拟南芥中的丝裂原活化蛋白激酶底物。

Plant signaling & behavior Pub Date : 2024-12-31 Epub Date: 2024-03-17 DOI: 10.1080/15592324.2024.2326238

Sunghwa Bahk, Nagib Ahsan, Jonguk An, Sun Ho Kim, Zakiyah Ramadany, Jong Chan Hong, Jay J Thelen, Woo Sik Chung

{"title":"Identification of mitogen-activated protein kinases substrates in Arabidopsis using kinase client assay.","authors":"Sunghwa Bahk, Nagib Ahsan, Jonguk An, Sun Ho Kim, Zakiyah Ramadany, Jong Chan Hong, Jay J Thelen, Woo Sik Chung","doi":"10.1080/15592324.2024.2326238","DOIUrl":"10.1080/15592324.2024.2326238","url":null,"abstract":"Mitogen-activated protein kinase (MPK) cascades are essential signal transduction components that control a variety of cellular responses in all eukaryotes. MPKs convert extracellular stimuli into cellular responses by the phosphorylation of downstream substrates. Although MPK cascades are predicted to be very complex, only limited numbers of MPK substrates have been identified in plants. Here, we used the kinase client (KiC) assay to identify novel substrates of MPK3 and MPK6. Recombinant MPK3 or MPK6 were tested against a large synthetic peptide library representing in vivo phosphorylation sites, and phosphorylated peptides were identified by high-resolution tandem mass spectrometry. From this screen, we identified 23 and 21 putative client peptides of MPK3 and MPK6, respectively. To verify the phosphorylation of putative client peptides, we performed in vitro kinase assay with recombinant fusion proteins of isolated client peptides. We found that 13 and 9 recombinant proteins were phosphorylated by MPK3 and MPK6. Among them, 11 proteins were proven to be the novel substrates of two MPKs. This study suggests that the KiC assay is a useful method to identify new substrates of MPKs.","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"19 1","pages":"2326238"},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10950278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140144907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel perspectives on plant behavior. 植物行为的新视角

Plant signaling & behavior Pub Date : 2024-12-31 Epub Date: 2024-11-04 DOI: 10.1080/15592324.2024.2419673

E Van Volkenburgh, E D Brenner

引用次数: 0

Exploring cotton SFR2's conundrum in response to cold stress. 探索棉花 SFR2 在应对寒冷胁迫时的难题。

Plant signaling & behavior Pub Date : 2024-12-31 Epub Date: 2024-06-05 DOI: 10.1080/15592324.2024.2362518

Samantha M Surber, Ngoc Pham Thien Thao, Cailin N Smith, Zachery D Shomo, Allison C Barnes, Rebecca L Roston

引用次数: 0

Physiological and transcriptomic analysis of tomato in response to sub-optimal temperature stress. 番茄对次优温度胁迫的生理和转录组分析。

Plant signaling & behavior Pub Date : 2024-12-31 Epub Date: 2024-03-21 DOI: 10.1080/15592324.2024.2332018

Huan Gao, Fengzhi Wu

{"title":"Physiological and transcriptomic analysis of tomato in response to sub-optimal temperature stress.","authors":"Huan Gao, Fengzhi Wu","doi":"10.1080/15592324.2024.2332018","DOIUrl":"10.1080/15592324.2024.2332018","url":null,"abstract":"Tomato (Solanum lycopersicum L.) is one of the most important economic crops in China. However, its quality and yield are susceptible to the adverse effects of low temperatures. In our study, two tomato cultivars, showing different tolerance to low temperatures, namely the cold-sensitive tomato cultivar (S708) and cold-tolerant tomato cultivar (T722), were grown at optimal (25/18°C) and sub-optimal (15/10°C) temperature conditions for 5 days. Our study aimed to explore the effect of sub-optimal temperature on fresh weight, chlorophyll content and chlorophyll fluorescence, soluble sugars and proline content of two tomato cultivars. Moreover, we employed RNA-Seq to analyze the transcriptomic response of tomato roots to sub-optimal temperature. The results revealed that S708 showed a more significant reduction in fresh weight, chlorophyll content, photochemical efficiency of PSII (YII), maximum quantum yield of PSII (Fv/Fm), photochemical quenching (qP) and electron transport rate (ETR) compared to T722 under the sub-optimal temperature condition. Notably, T722 maintained higher level of soluble sugars and proline in comparison to S708 uner sub-optimal temperature. RNA-seq data showed that up-regulated DEGs in both tomato cultivars were involved in \"plant-pathogen interaction\", \"MAPK signaling pathway\", \"plant hormone signal transduction\", and \"phosphatidylinositol signaling system\". Furthermore, \"Amino sugar and nucleotide sugar metabolism\" pathway was enriched only in T722. Moreover, under sub-optimal temperature, transcription factor genes and osmoregulation genes showed varying degrees of response in both tomato cultivars. Conclusion: In summary, our results offer detailed insights into the response characteristics of tomato to sub-optimal temperature, providing valuable references for the practical management of tomato crops under sub-optimal temperature condition.","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"19 1","pages":"2332018"},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10962623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140178419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Luciferase-mediated assay to detect the PAMP-triggered gene expression in transgenic Nicotiana benthamiana. 以荧光素酶为介导的检测方法，用于检测转基因烟草中 PAMP 触发的基因表达。

Plant signaling & behavior Pub Date : 2024-12-31 Epub Date: 2024-11-25 DOI: 10.1080/15592324.2024.2411918

Ola Barakat, Fushuang Zhang, Mengzhu Zeng, Yuanchao Wang

{"title":"Luciferase-mediated assay to detect the PAMP-triggered gene expression in transgenic Nicotiana benthamiana.","authors":"Ola Barakat, Fushuang Zhang, Mengzhu Zeng, Yuanchao Wang","doi":"10.1080/15592324.2024.2411918","DOIUrl":"10.1080/15592324.2024.2411918","url":null,"abstract":"Luciferase is one of the bioluminescence-producing agents, which was widely used as a reporter enzyme for constructing bioassay systems to study gene expression with high accuracy and within a broad dynamic spectrum. Perception of pathogen associated molecular patterns (PAMPs) in plants often lead to significant transcriptional changes. The transcriptional changes of defense-related genes are often used as a marker to assay PAMP-triggered plant immune response. In this study, we showed that the marker gene CYP71D20 was rapidly activated in Nicotiana benthamiana upon treatment with the bacterial PAMP flg22 and the Phytophthora elicitin INF1. In addition, we generated transgenic N. benthamiana using the luciferase as a reporter gene to analyze CYP71D20 gene expression upon PAMP treatment. The transgenic line carrying the luciferase gene driven by CYP71D20 promoter was treated with the bacterial PAMP flg22 or Phytophthora elicitin INF1. Transcriptional activation of CYP71D20 was measured by monitoring the luciferase activity. The results showed that the LUC activity was increased after treatment with different PAMPs, indicating that the CYP71D20-promotor luciferase assay can be used to study the PAMP-triggered gene expression in N. benthamiana.","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"19 1","pages":"2411918"},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11591477/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant ultrasound detection: a cost-effective method for identifying plant ultrasonic emissions. 植物超声波探测：一种识别植物超声波发射的经济有效的方法。

Plant signaling & behavior Pub Date : 2024-12-31 Epub Date: 2024-02-12 DOI: 10.1080/15592324.2024.2310974

Luca Bonisoli, Ivano Pelicella, Laura Arru

引用次数: 0

Proteome-wide analysis reveals G protein-coupled receptor-like proteins in rice (Oryza sativa). 全蛋白质组分析揭示了水稻（Oryza sativa）中的 G 蛋白偶联受体样蛋白。

Plant signaling & behavior Pub Date : 2024-12-31 Epub Date: 2024-06-21 DOI: 10.1080/15592324.2024.2365572

Dinesh K Yadav, Gyan Prakash Srivastava, Ananya Singh, Madhavi Singh, Neelam Yadav, Narendra Tuteja

{"title":"Proteome-wide analysis reveals G protein-coupled receptor-like proteins in rice (Oryza sativa).","authors":"Dinesh K Yadav, Gyan Prakash Srivastava, Ananya Singh, Madhavi Singh, Neelam Yadav, Narendra Tuteja","doi":"10.1080/15592324.2024.2365572","DOIUrl":"10.1080/15592324.2024.2365572","url":null,"abstract":"G protein-coupled receptors (GPCRs) constitute the largest family of transmembrane proteins in metazoans that mediate the regulation of various physiological responses to discrete ligands through heterotrimeric G protein subunits. The existence of GPCRs in plant is contentious, but their comparable crucial role in various signaling pathways necessitates the identification of novel remote GPCR-like proteins that essentially interact with the plant G protein α subunit and facilitate the transduction of various stimuli. In this study, we identified three putative GPCR-like proteins (OsGPCRLPs) (LOC_Os06g09930.1, LOC_Os04g36630.1, and LOC_Os01g54784.1) in the rice proteome using a stringent bioinformatics workflow. The identified OsGPCRLPs exhibited a canonical GPCR 'type I' 7TM topology, patterns, and biologically significant sites for membrane anchorage and desensitization. Cluster-based interactome mapping revealed that the identified proteins interact with the G protein α subunit which is a characteristic feature of GPCRs. Computational results showing the interaction of identified GPCR-like proteins with G protein α subunit and its further validation by the membrane yeast-two-hybrid assay strongly suggest the presence of GPCR-like 7TM proteins in the rice proteome. The absence of a regulator of G protein signaling (RGS) box in the C- terminal domain, and the presence of signature motifs of canonical GPCR in the identified OsGPCRLPs strongly suggest that the rice proteome contains GPCR-like proteins that might be involved in signal transduction.","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"19 1","pages":"2365572"},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11195488/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141433737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitric oxide in plants: an insight on redox activity and responses toward abiotic stress signaling. 植物中的一氧化氮：对氧化还原活动和非生物胁迫信号反应的认识。

Plant signaling & behavior Pub Date : 2024-12-31 Epub Date: 2024-01-08 DOI: 10.1080/15592324.2023.2298053

Khushboo Khator, Suman Parihar, Jan Jasik, Gyan Singh Shekhawat

{"title":"Nitric oxide in plants: an insight on redox activity and responses toward abiotic stress signaling.","authors":"Khushboo Khator, Suman Parihar, Jan Jasik, Gyan Singh Shekhawat","doi":"10.1080/15592324.2023.2298053","DOIUrl":"10.1080/15592324.2023.2298053","url":null,"abstract":"Plants, as sessile organisms, are subjected to diverse abiotic stresses, including salinity, desiccation, metal toxicity, thermal fluctuations, and hypoxia at different phases of plant growth. Plants can activate messenger molecules to initiate a signaling cascade of response toward environmental stresses that results in either cell death or plant acclimation. Nitric oxide (NO) is a small gaseous redox-active molecule that exhibits a plethora of physiological functions in growth, development, flowering, senescence, stomata closure and responses to environmental stresses. It can also facilitate alteration in protein function and reprogram the gene profiling by direct or indirect interaction with different target molecules. The bioactivity of NO can be manifested through different redox-based protein modifications including S-nitrosylation, protein nitration, and metal nitrosylation in plants. Although there has been considerable progress in the role of NO in regulating stress signaling, still the physiological mechanisms regarding the abiotic stress tolerance in plants remain unclear. This review summarizes recent advances in understanding the emerging knowledge regarding NO function in plant tolerance against abiotic stresses. The manuscript also highlighted the importance of NO as an abiotic stress modulator and developed a rational design for crop cultivation under a stress environment.","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"19 1","pages":"2298053"},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10793691/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139405809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0