Plant signaling & behavior最新文献

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Expression characteristics of CsPG23 in citrus and analysis of its interacting protein. CsPG23在柑橘中的表达特征及其互作蛋白分析。
Plant signaling & behavior Pub Date : 2025-12-01 Epub Date: 2025-05-22 DOI: 10.1080/15592324.2025.2508418
Qing He, Xiao He
{"title":"Expression characteristics of <i>CsPG23</i> in citrus and analysis of its interacting protein.","authors":"Qing He, Xiao He","doi":"10.1080/15592324.2025.2508418","DOIUrl":"10.1080/15592324.2025.2508418","url":null,"abstract":"<p><p>Exploring the resistance genes of citrus to Huanglongbing (HLB) is the foundation and key to citrus disease-resistant breeding. Through the analysis of comparative transcriptome data, we identified six cell wall degradation genes that respond to citrus infection with <i>Ca</i>Las. We selected one of the genes with high differential expression levels and cloned it, naming it <i>CsPG23</i>. The subcellular localization results of tobacco indicated that the <i>CsPG23</i> protein is localized in the nucleus, cytoplasm, and cell membrane. Real-time fluorescence quantitative PCR (RT-qPCR) analysis showed that the expression of <i>CsPG23</i> is related to variety tolerance, tissue location, and symptom development. In addition, we constructed overexpression and silencing vectors for <i>CsPG23</i> and obtained <i>CsPG23</i> silencing plants, overexpression and silencing hairy roots, and analyzed the expression characteristics of <i>CsPG23</i> in response to SA, JA, MeSA and H<sub>2</sub>O<sub>2</sub> induction through RT-qPCR. Using Protein-Protein Interaction (PPI) to predict and screen for a citrus protein CsAGD8 that may interact with CsPG23, and preliminarily verifying its interaction with CsPG23 protein through Yeast Two-hybrid (Y2H). We constructed overexpression and silencing vectors for <i>CsAGD8</i> and obtained <i>CsAGD8</i> overexpression and silencing hairy roots. In summary, it is indicated that CsPG23 may interact with CsAGD8 in response to <i>Ca</i>Las infection.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"20 1","pages":"2508418"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144129837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
APE1 localizes to chloroplast stromules and interacts with ATI1 in Arabidopsis. 在拟南芥中,APE1定位于叶绿体基质并与ATI1相互作用。
Plant signaling & behavior Pub Date : 2025-12-01 Epub Date: 2025-05-30 DOI: 10.1080/15592324.2025.2511830
Zihan Ge, Yunhe Jing, Jiaoyu Zhu, Li-En Yang, Shan Lu, Yinyin Deng
{"title":"APE1 localizes to chloroplast stromules and interacts with ATI1 in Arabidopsis.","authors":"Zihan Ge, Yunhe Jing, Jiaoyu Zhu, Li-En Yang, Shan Lu, Yinyin Deng","doi":"10.1080/15592324.2025.2511830","DOIUrl":"10.1080/15592324.2025.2511830","url":null,"abstract":"<p><p>Chloroplast stromules are dynamic tubular extensions implicated in inter-organelle communication and stress responses in plants. However, the molecular mechanisms underlying stromule-mediated processes remain largely unexplored. In this study, we investigated the localization and function of ACCLIMATION OF PHOTOSYNTHESIS TO ENVIRONMENT1 (APE1) and its interaction with the autophagy-related protein ATG8-INTERACTING PROTEIN1 (ATI1) in <i>Arabidopsis thaliana</i>. Expression analysis revealed that <i>APE1</i> is predominantly expressed in rosette leaves. Using yeast two-hybrid and luciferase complementation assays, we confirmed a direct interaction between APE1 and ATI1. Confocal microscopy demonstrated that APE1 localizes both within chloroplasts and stromules, co-localizing with stromule markers, while sub-organelle fractionation indicated APE1 enrichment in the thylakoid and inner envelope membranes. Our findings suggest that APE1, through its interaction with ATI1, may play a crucial role in stromule-mediated signaling and the selective autophagic degradation of chloroplast proteins during stress acclimation. This work provides new insights into the functional dynamics of stromules and highlights the importance of the APE1-ATI1 interaction in chloroplast-nucleus communication under environmental stress.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"20 1","pages":"2511830"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12128654/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Putrescine mitigates NaCl-induced stress by modulating gene expression, antioxidants, and ethylene level in tomato. 腐胺通过调节番茄的基因表达、抗氧化剂和乙烯水平来减轻nacl诱导的应激。
Plant signaling & behavior Pub Date : 2025-12-01 Epub Date: 2025-06-16 DOI: 10.1080/15592324.2025.2515431
Yalaga Rama Rao, Priya Yadav, Varsha Rani, Devayani Muley, Ranjan Kumar Sahoo, Brajendra, Shashi Bhushan Kumar, Ritu Gill, Sarvajeet Singh Gill, Mohammad Wahid Ansari, Narendra Tuteja
{"title":"Putrescine mitigates NaCl-induced stress by modulating gene expression, antioxidants, and ethylene level in tomato.","authors":"Yalaga Rama Rao, Priya Yadav, Varsha Rani, Devayani Muley, Ranjan Kumar Sahoo, Brajendra, Shashi Bhushan Kumar, Ritu Gill, Sarvajeet Singh Gill, Mohammad Wahid Ansari, Narendra Tuteja","doi":"10.1080/15592324.2025.2515431","DOIUrl":"10.1080/15592324.2025.2515431","url":null,"abstract":"<p><p>Plant development and productivity are significantly hindered by salt stress, leading to substantial financial losses in the agriculture sector. Salinity stress negatively impacts the overall growth, physiology, and metabolism of plants. Specifically, NaCl stress is particularly harmful to tomato plants, causing suppression of seedling growth, accumulation of sodium (Na<sup>+</sup>) and chloride (Cl<sup>-</sup>) ions, disrupted ion homeostasis, reduced proline and chlorophyll content, and impairment of antioxidant enzyme systems. This research aimed to investigate the role of exogenous putrescine (PUT) application on tomato (<i>Solanum lycopersicum</i> L.) seedlings under NaCl stress (250 mm) to determine its potential protective effects. Various physio-biochemical attributes were estimated using precise protocols for NaCl-treated, PUT-treated, and untreated controlled tomato seedlings also analyzed for the expression of ACS1, NHX1, HKT1;2, and SOS1 genes. Additionally, ACC synthase activity, ethylene content, electrolyte leakage, proline content, Na<sup>+</sup> and potassium (K<sup>+</sup>) ion content, lycopene content, and antioxidant enzyme activities were examined. Results indicated that PUT application enhanced the expression of ACS1, NHX1, HKT1;2, and SOS1 genes increase the ACC synthase activity, ethylene content, proline content, and Na<sup>+</sup> and K<sup>+</sup> ion content, while reducing electrolyte leakage. Furthermore, PUT application significantly increased the activity of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR), as well as other morphological parameters. Overall, our research demonstrated the potential benefits of PUT applications for enhancing crop growth and improving salt stress tolerance, which are crucial for agronomy.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"20 1","pages":"2515431"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12184179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144311145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Francis Darwin (1848-1925): the biologist in the shadow of father Charles. 弗朗西斯·达尔文(1848-1925):受父亲查尔斯影响的生物学家。
Plant signaling & behavior Pub Date : 2025-12-01 Epub Date: 2025-05-30 DOI: 10.1080/15592324.2025.2509264
Ulrich Kutschera, František Baluška
{"title":"Francis Darwin (1848-1925): the biologist in the shadow of father Charles.","authors":"Ulrich Kutschera, František Baluška","doi":"10.1080/15592324.2025.2509264","DOIUrl":"10.1080/15592324.2025.2509264","url":null,"abstract":"<p><p>This year marks the 100th anniversary of the death of Sir Francis Darwin (1848-1925), the biologist/naturalist, musicologist, and biographer/philosopher of science in the shadow of father Charles (1809-1882). Francis was the seventh child and third son of the British couple. Here, we present a concise biography of Francis Darwin, based on his neglected \"Recollections\" published in his book <i>Springtime and other Essays</i> (1920). We summarize the scientific legacy of the younger Darwin with a focus on the \"root-brain-hypotheses\", as described in the 1880- \"Darwin & Darwin book <i>The Power of Movements in Plants</i>, his extensive work on water-loss (transpiration) in plants via the stomata, and the Francis D.-papers on music instruments. In addition, we shed new light on the well-known \"Darwin-Julius Sachs\"-controversy of the 1880s. Finally, we document that the Biologist Francis Darwin was also a musician and polymath, with broad expertise in the natural sciences and the humanities alike.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"20 1","pages":"2509264"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12128675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cloning and function analysis of ZmICE1a, a contributor to the melioration of maize kernel traits. 玉米籽粒性状改良基因ZmICE1a的克隆与功能分析
Plant signaling & behavior Pub Date : 2025-12-01 Epub Date: 2025-07-02 DOI: 10.1080/15592324.2025.2521320
Yifei Xiao, Liang Tu, Yulin Jiang, Pengfei Liu, Xiangyang Guo, Angui Wang, Yunfang Zhu, Xuefeng Lu, Zehui Chen, Xun Wu
{"title":"Cloning and function analysis of <i>ZmICE1a</i>, a contributor to the melioration of maize kernel traits.","authors":"Yifei Xiao, Liang Tu, Yulin Jiang, Pengfei Liu, Xiangyang Guo, Angui Wang, Yunfang Zhu, Xuefeng Lu, Zehui Chen, Xun Wu","doi":"10.1080/15592324.2025.2521320","DOIUrl":"10.1080/15592324.2025.2521320","url":null,"abstract":"<p><p>Kernel traits are important factors in determining maize yield. Gene mining and clarification of relevant gene functions associated with kernel traits is beneficial for breeding high-yield maize varieties. In our previous research, a critical quantitative trait locus (QTL), <i>qKWEI3.1</i>, associated with kernel weight was mapped using a maize F<sub>2:3</sub> population derived from the parental lines SCML0849 and ZNC442. In the present study, <i>qKWEI3.1</i> was fine-mapped, the <i>ZmICE1a</i> gene was cloned, and the relevant functions of <i>ZmICE1a</i> were dissected. The results showed that plants overexpressing <i>ZmICE1</i>a exhibited a shorter reproductive period, increased plant height, greater stem diameter, higher photosynthetic efficiency, and meliorated kernel traits. Transcriptome analysis revealed that <i>ZmICE1a</i> overexpression mediated differentially expressed genes (DEGs) such as <i>SNRK2-10</i>, the E3 ubiquitin-protein ligase <i>AIP2</i>, <i>Pho1</i>, and <i>Pho2</i>. Gene ontology and KEGG pathway enrichment analyses revealed that the DEGs were involved in the abscisic acid signaling pathway, starch, and sucrose metabolism. These results suggest that <i>ZmICE1a</i> is a critical, positive regulator promoting plant growth and meliorating kernel traits. The findings of this study have important implications for the improvement of grain yield through the application of genetic engineering in maize breeding.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"20 1","pages":"2521320"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12233868/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144546709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Aux/IAAs: specificity and redundancy. 辅助/IAAs:特异性和冗余性。
Plant signaling & behavior Pub Date : 2025-12-01 Epub Date: 2025-07-10 DOI: 10.1080/15592324.2025.2530541
Qiming Wen, Qian Gong, Huaying Yu, Panyu Yang
{"title":"Aux/IAAs: specificity and redundancy.","authors":"Qiming Wen, Qian Gong, Huaying Yu, Panyu Yang","doi":"10.1080/15592324.2025.2530541","DOIUrl":"10.1080/15592324.2025.2530541","url":null,"abstract":"<p><p><i>Auxin/Indole-3-Acetic Acids</i>(<i>Aux/IAAs</i>), a class of early auxin-responsive genes, encode short-lived nuclear proteins that play pivotal roles in auxin signaling. In vascular plants, <i>Aux/IAA</i> genes form large families-such as the 29 members in Arabidopsis, exhibiting both functional redundancy and specificity. Canonical Aux/IAA proteins contain four conserved domains and mediate nuclear auxin response by interacting with Transport Inhibitor Response 1/Auxin Signaling F-box (TIR1/AFB) auxin receptors and Auxin Response Factor (ARF) transcription factors. Loss- and gain-of-function mutants have been instrumental in dissecting the roles of individual <i>Aux/IAAs</i>. Recent studies have also uncovered the mechanism of non-canonical Aux/IAAs, which lack one or more conserved domains and regulate auxin signaling through distinct pathways. This review summarizes the structural features of Aux/IAA proteins, the functional diversity of non-canonical members, the phenotypic effects of their mutants, and their expression patterns. These findings reveal a hierarchical regulatory network of the <i>Aux/IAA</i> gene family in auxin signaling - balancing robustness through functional redundancy and precision and flexibility through member-specific functions in plant growth and development.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"20 1","pages":"2530541"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12247088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144602689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Science behind vegetable aromas: types, synthesis, and influencing factors of volatile compounds. 蔬菜香气背后的科学:挥发性化合物的类型、合成和影响因素。
Plant signaling & behavior Pub Date : 2025-12-01 Epub Date: 2025-07-12 DOI: 10.1080/15592324.2025.2527958
Chenxi Ji, Chenxu Liu, Rongqing Wang, Meiying Ruan, Zhuping Yao, Hongjian Wan, Zhimiao Li, Yuan Cheng, Qingjing Ye
{"title":"The Science behind vegetable aromas: types, synthesis, and influencing factors of volatile compounds.","authors":"Chenxi Ji, Chenxu Liu, Rongqing Wang, Meiying Ruan, Zhuping Yao, Hongjian Wan, Zhimiao Li, Yuan Cheng, Qingjing Ye","doi":"10.1080/15592324.2025.2527958","DOIUrl":"10.1080/15592324.2025.2527958","url":null,"abstract":"<p><p>Volatile organic compounds (VOCs) are key determinants of the aroma characteristics in vegetable crops. They not only directly affect consumer sensory experiences but also play important roles in plant - environment interactions. With the continuous advancement of analytical technologies, the composition, biosynthetic pathways, and regulatory mechanisms of VOCs in vegetables have become research hotspots in recent years. This review systematically summarizes the major types of VOCs (aldehydes, esters, terpenes, and sulfur-containing compounds) in vegetable crops and the types of aromas they present. It further explores their biosynthetic pathways, such as those with fatty acids, amino acids, and carotenoids as precursors, as well as the synthesis pathways of terpenoids and phenylpropanoids. In addition, this review covers commonly used enrichment and analytical techniques, including HS-SPME, GC-MS, GC×GC-TOF-MS, as well as the various factors that influence VOC composition - such as genotype, developmental stage, environmental conditions, and postharvest handling. This work provides theoretical support and research directions for the breeding and industrial application of vegetable varieties with improved flavor quality. Future studies should further investigate the functions of VOCs, their regulatory networks, and their roles in plant ecological adaptation.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"20 1","pages":"2527958"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12258172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144621577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome-wide identification and analysis of the NF-Y transcription factor family reveal its potential roles in tobacco (Nicotiana tabacum L.). NF-Y转录因子家族的全基因组鉴定和分析揭示了其在烟草中的潜在作用。
Plant signaling & behavior Pub Date : 2025-12-01 Epub Date: 2025-01-16 DOI: 10.1080/15592324.2025.2451700
Zhen Tian, Luyao Xue, Jincun Fu, Wenting Song, Baojian Wang, Jinhao Sun, Xiujiang Yue, Fanrui Cheng, Jingjing Mao, Jiangtao Chao, Dawei Wang, Shaopeng Li
{"title":"Genome-wide identification and analysis of the NF-Y transcription factor family reveal its potential roles in tobacco (<i>Nicotiana tabacum</i> L.).","authors":"Zhen Tian, Luyao Xue, Jincun Fu, Wenting Song, Baojian Wang, Jinhao Sun, Xiujiang Yue, Fanrui Cheng, Jingjing Mao, Jiangtao Chao, Dawei Wang, Shaopeng Li","doi":"10.1080/15592324.2025.2451700","DOIUrl":"10.1080/15592324.2025.2451700","url":null,"abstract":"<p><p>Nuclear Factor Y (NF-Y) represents a group of transcription factors commonly present in higher eukaryotes, typically consisting of three subunits: NF-YA, NF-YB, and NF-YC. They play crucial roles in the embryonic development, photosynthesis, flowering, abiotic stress responses, and other essential processes in plants. To better understand the genome-wide NF-Y domain-containing proteins, the protein physicochemical properties, chromosomal localization, synteny, phylogenetic relationships, genomic structure, promoter <i>cis</i>-elements, and protein interaction network of NtNF-Ys in tobacco (<i>Nicotiana tabacum</i> L.) were systematically analyzed. In this study, we identified 58 NtNF-Ys in tobacco, respectively, and divided into three subfamilies corresponding to their phylogenetic relationships. Their tissue specificity and expression pattern analyses for leaf development, drought and saline-alkali stress, and ABA response were carried out using RNA-seq or qRT-PCR. These findings illuminate the role of NtNF-Ys in regulating plant leaf development, drought and saline-alkali stress tolerance, and ABA response. This study offers new insights to enhance our understanding of the roles of NtNF-Ys and identify potential genes involved in leaf development, as well as drought and saline-alkali stress tolerance of plants.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"20 1","pages":"2451700"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11740682/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143019103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Aba-induced active stomatal closure in bulb scales of Lanzhou lily. aba诱导兰州百合鳞茎气孔主动关闭。
Plant signaling & behavior Pub Date : 2025-12-01 Epub Date: 2024-12-28 DOI: 10.1080/15592324.2024.2446865
Lei Gong, Hai-Qing Liu, Ye Hua, Ya-Yun Zhang, Md Mahadi Hasan
{"title":"Aba-induced active stomatal closure in bulb scales of Lanzhou lily.","authors":"Lei Gong, Hai-Qing Liu, Ye Hua, Ya-Yun Zhang, Md Mahadi Hasan","doi":"10.1080/15592324.2024.2446865","DOIUrl":"https://doi.org/10.1080/15592324.2024.2446865","url":null,"abstract":"<p><p>Abscisic acid (ABA) mediated stomatal closure is a highly effective mode of active stomatal regulation under drought stress. Previous studies on stomatal regulation have primarily focused on the leaves of vascular plants, while research on the stomatal behavior of bulbous plants remains unknown. In addition, ABA-induced stomatal regulation in bulbs has yet to be explored. Therefore, we aim to investigate the ABA-induced active regulation in the bulb of the Lanzhou lily (<i>Lilium davidii</i> var. unicolor). The morphological characteristics of epidermal strips were analyzed along with a stomatal aperture assay to investigate the bulb's stomatal response to ABA. Moreover, the mechanism of ABA signaling was explored using treatments with ABA signaling chemicals and corresponding scavengers. This study revealed that stomata are mainly distributed on the upper part and outer surface of the bulb. The guard cells of the lily bulb are inflated, and the stomata have a nearly circular shape with relatively low stomatal density. Exogenous ABA was found to induce varying degrees of stomatal closure in a dose-dependent manner, with significant stomatal aperture reduction observed after treatment with 10 µM ABA. Overall, the study indicated that both hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and nitric oxide (NO) are involved in the ABA-induced stomatal closure process, with H<sub>2</sub>O<sub>2</sub> functioning as an upstream component of NO.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"20 1","pages":"2446865"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142901551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antifungal and enzymatic activities of endophytic fungi associated with Tamarix nilotica. 柽柳内生真菌抗真菌活性及酶活性研究。
Plant signaling & behavior Pub Date : 2025-12-01 Epub Date: 2024-12-29 DOI: 10.1080/15592324.2024.2439250
Helal F Al-Harthi
{"title":"Antifungal and enzymatic activities of endophytic fungi associated with <i>Tamarix nilotica</i>.","authors":"Helal F Al-Harthi","doi":"10.1080/15592324.2024.2439250","DOIUrl":"10.1080/15592324.2024.2439250","url":null,"abstract":"<p><p>Fungal endophytes were recovered from <i>Tamarix nilotica</i> (Tamaricaceae) roots and stems collected in Taif, Saudi Arabia. A total of 49 different taxa were identified. The overall colonization rate of root and stem segments was 30.6%. A total of 49 isolates were collected and categorized into 21 operational taxonomic units using the rRNA gene's internal transcribed spacer region. The most prevalent species were <i>Penicillium chrysogenum</i> (16 isolates), Fungal sp. (12), and <i>Alternaria alternata</i> (10). Forty-nine isolates were investigated for antifungal activity against <i>Fusarium solani</i> and <i>Rhizoctonia solani</i>; all tested isolates showed antifungal activity against <i>Fusarium solani</i>, while 43 isolates showed antifungal activity against <i>Rhizoctonia solani</i>. The most potent antifungal agents against <i>Fusarium solani</i> and <i>Rhizoctonia solani</i> are <i>Aspergillus ochraceus</i> (2 isolates) and <i>Penicillium chrysogenum</i> (16 isolates). Endophytic isolates collected during this experiment were evaluated to produce amylase, cellulase, lipase, and protease enzymes. Among the strains examined for enzymatic potentials (4 enzymes), 26 strains (53% of the total strains) produce only one enzyme. <i>Penicillium chrysogenum</i> (TUPc2, 3, and 4) produced the highest amount of the four enzymes that were examined.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"20 1","pages":"2439250"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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