Plant signaling & behavior最新文献

{"title":"Overexpression of <i>ORP1C</i> gene increases the rice resistance to <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> through negatively regulating transcription activator-like effectors translocation.","authors":"Hongtao Ji, Lan Zhou, Ruibin Yang, Mingliang Xu, Hengjie Qian, Jingyi Tong, Mengjie Sun","doi":"10.1080/15592324.2024.2441864","DOIUrl":"https://doi.org/10.1080/15592324.2024.2441864","url":null,"abstract":"<p><p>Bacterial leaf blight (BLB) caused by <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>) has shown a high incidence rate in rice fields in recent years. Rice resistance breeding is considered as the most effective method for achieving economical and sustainable management of BLB disease. The essential basis for resistance breeding is rooted in the exploration of rice resistance genes and the clarification of the molecular mechanisms that underlie <i>Xoo</i> resistance. In our previous research, we showed that <i>Xanthomonas</i> outer protein XopZ and rice oxysterol-binding related protein ORP1C collaboratively regulate the compatible interaction between <i>Xoo</i> strain PXO99 and Nipponbare rice, but the deeper regulatory mechanisms remain unknown. In this study, we successfully constructed <i>ORP1C</i> overexpression rice using the plant binary expression vector pCAMBIA1301. Through a series of virulence and effector translocation detections in <i>Xoo</i>-rice interactions, we revealed that overexpression of the <i>ORP1C</i> gene largely increases rice resistance to multiple <i>Xoo</i> strains from different countries and regions. Mechanistically, ORP1C plays a <i>Xoo</i> resistant role through negatively regulating transcription activator-like effectors (TALEs) translocation, <i>ORP1C</i> has become a potential candidate gene resource for disease-resistant breeding in rice. Further studies also indicated that XopZ and ORP1C collaboratively regulate the compatible interaction of PXO99-Nipponbare by modulating TALEs translocation.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"20 1","pages":"2441864"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The inhibitory activities of two compounds from <i>Securidaca longepedunculata</i> Fresen on the acetylcholinesterase from wheat pest <i>Schizaphis graminum</i> Rondani: <i>in silico</i> analysis.","authors":"Rasmané Guiré, Pousbila Salo, Eliasse Zongo, Mohamed Fawzy Ramadan, Benjamin Kouliga Koama, Roland Nag-Tiero Meda, Fahad Al-Asmari, Muhammad Abdul Rahim","doi":"10.1080/15592324.2024.2444311","DOIUrl":"10.1080/15592324.2024.2444311","url":null,"abstract":"<p><p>Wheat is the third most widely consumed cereal in the world, after maize and rice. However, it is regularly attacked by the wheat aphid (<i>Schizaphis graminum</i>), causing considerable damage to wheat crops. The acetylcholinesterase enzyme, which plays a key role in the transmission of the synaptic cholinergic signal, has emerged as a promising target for the development of pest control strategies. Inhibition of this enzyme leads to the paralysis or even death of the aphid. The objective of this study is to identify the bioactive compounds in <i>Securidaca longepedunculata (S. longepedunculata)</i> that are capable of interacting with acetylcholinesterase from <i>Schizaphis graminum</i> and inhibiting its activity. Furthermore, a computer simulation of these compounds in interaction with the key protein was conducted. First, the secondary metabolites of <i>S. longepedunculata</i> were selected on the basis of GC-MS data available from specific reference sources. Subsequently, the compounds were subjected to virtual screening based on their docking scores in order to identify those with inhibitory properties. The compounds with the highest scores were subjected to molecular dynamics simulation over a 50 ns trajectory. Subsequently, MMGBSA free energy calculations were conducted. The results demonstrated that eight compounds exhibited inhibitory properties, four of which (echimidine, populin, salidroside, and farrerol) demonstrated superior stabilizing effects on proteins compared to the remaining compounds. In terms of free energy by MMGBSA and molecular simulation, it was observed that echimidine and populin formed robust and stable hydrogen bonds with the amino acids of the acetylcholinesterase enzyme. This study identifies and attempts to validate the potential inhibitory activities of echimidine and populin against acetylcholinesterase, with a view to developing potent insecticides and unique treatment strategies.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"20 1","pages":"2444311"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11660410/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142866859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the efficacy of drought tolerant, IAA-producing plant growth-promoting rhizobacteria for sustainable agriculture.","authors":"Malika Uzma, Atif Nisar, Atia Iqbal, Shahida Hasnain, Mohamed H Mahmoud, Muhammad Abdul Rahim, Tehseen Gull, Roberto Castro-Muñoz, Eliasse Zongo","doi":"10.1080/15592324.2025.2452331","DOIUrl":"10.1080/15592324.2025.2452331","url":null,"abstract":"<p><p>The growing human population and abiotic stresses pose significant threats to food security, with PGPR favorable as biofertilizers for plant growth and stress relief. In one study, soil samples from both cultivated and uncultivated plants in various cities were used to isolate rhizobacterial populations. Using 50 soil samples from both cultivated and uncultivated plants, isolated rhizobacterial populations were screened for various biochemical changes, PGP activities and morphological characteristics. A total of 199 rhizobacteria were isolated and screened for IAA production. The strain M28 produced maximum IAA 378.44 ± 2.5 µg ml^-1, M9 formed only 34.72 ± 0.15 µg ml^-1. About 19% of IAA producers were isolated from Multan, 18% Lahore, 15% from soils of Faisalabad and Sheikhupura, while 7% from Gujrat. The 21 isolates were drought tolerant to -0.14Mpa, 14 of those were PSB and 15 were N fixers. In PGP traits, maximum zinc solubility was expressed by M4 as 2 ± 0.5 cm of zone. The strain M22 produced amount of HCN, 40.12 ± 0.052 ppm. All isolates showed diverse behavior in biocompatibility, motility patterns and hydrophobicity. Selected drought tolerant strains were genetically identified by ribotyping. Multitrait PGPR could be effective biofertilizers rather than with single trait. The strain M28 having highest production of IAA, was gelatinase, methyl red positive and was also capable of nitrogen fixation. Moreover, it had maximum swimming (8.9 mm) and swarming (8.7 mm) activities after 24 h, indicating its best PGP traits for future use.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"20 1","pages":"2452331"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11740683/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The biochemical and molecular mechanisms of plants: a review on insect herbivory.","authors":"Afeez Adesina Adedayo, Richard Musser, Mari Aanaenson, Olubukola Oluranti Babalola","doi":"10.1080/15592324.2024.2439248","DOIUrl":"https://doi.org/10.1080/15592324.2024.2439248","url":null,"abstract":"<p><p>Biochemical and molecular mechanisms have been essential mechanisms to reduce various insect attacks on plants. The biochemical methods are wide involving direct and indirect defenses. The defensive chemical substances are secreted effectively to the wound caused by the herbivores (insects and phytopathogens) on plants. Plants responded by producing VOCs which draw the natural enemies of the insects and phytopathogens. The progress observed in the cognition of the stimulus in plants and their potential to control the responses is characterized by the modification observed in molecular mechanisms which shifts our attention to the development of the endogenous resistance methods of preserving crops. The main objective of implementing a biotechnological mechanism in crop production is to employ durable and multimechanistic alternatives to insect pests via the stimulus the plant produces upon encountering the insect attack.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"20 1","pages":"2439248"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142901552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Do strigolactones play a role in the ascent and attachment behavior of <i>Pisum sativum</i>?","authors":"Bianca Bonato, Tom Bennett, Silvia Guerra, Sara Avesani, Umberto Castiello","doi":"10.1080/15592324.2024.2447455","DOIUrl":"https://doi.org/10.1080/15592324.2024.2447455","url":null,"abstract":"<p><p>Strigolactones (SLs) are signaling compounds made by plants. They play a crucial role in acting as long-distance signals from root to shoot to coordinate shoot growth with root environmental conditions. Here, we test whether and how SLs play a role in the climbing behavior of pea plants by studying the circumnutation of the tendrils using three-dimensional (3D) kinematical analysis. To assess this, we compare the typical behavior of <i>P. sativum</i>, a wild-type plant that produces and perceives SLs, with mutants defective in SLs synthesis or signaling, known as <i>ramosus</i>(<i>rms</i>) mutants. The results indicate that mutant plants seem unable to locate and grasp a potential support. Their movement appears to be disoriented and much less energized. We contend that this research opens new avenues for exploring SLs' role in plant behavior, a novel lens through which the role of SLs in root-to-shoot communication can be observed and analyzed.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"20 1","pages":"2447455"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142924211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hormonomics profiles revealed the mechanisms of cold stratification in breaking the dormancy during seed germination and emergence process of Polygonatum sibiricum Red.","authors":"Haiqing Liu, Jie Yuan, Hanjin Wu, Xiaobin Ou, Zhengkun Liu, Xiuli Liu, Shuyan He","doi":"10.1080/15592324.2024.2447460","DOIUrl":"https://doi.org/10.1080/15592324.2024.2447460","url":null,"abstract":"<p><p><i>Polygonatum sibiricum</i> Red, known as Huangjing in Chinese, is a perennial plant valued in traditional Chinese medicine and is a nutritional food ingredient. With increasing market demand outpacing wild resource availability, cultivation has become essential for sustainable production. However, the cultivation of <i>P. sibiricum</i> is challenged by the double dormancy characteristics of seeds, which include embryo and physiological dormancy. This affected the germination of seeds and the establishment of seedlings. This study investigates the role of plant hormones in breaking seed dormancy and regulating germination and emergence in <i>P. sibiricum</i>. We found that cold stratification at 4°C for over 70 d significantly alleviates seed dormancy, associated with changes in endogenous hormone levels. Auxin, gibberellin, abscisic acid, cytokinin, salicylic acid, jasmonic acid, and ethylene were identified as key players in these processes. Exogenous applications of GA3 and 2-coumarate (2-hydroxycinnamic acid) significantly enhanced seed germination, while 6-BA and GA3 promoted corm growth and development. In conclusion, our research provides insights into the hormonal regulation of seed dormancy and germination in <i>P. sibiricum</i>, offering valuable strategies for improving cultivation practices. Further studies are needed to explore the specific mechanisms of hormone interactions and to develop optimized germination and seedling establishment strategies for this medicinally important plant.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"20 1","pages":"2447460"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142904671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly expressed cell wall genes contribute to robustness of sepal size.","authors":"Diego A Hartasánchez, Mathilde Dumond, Nelly Dubrulle, Françoise Monéger, Arezki Boudaoud","doi":"10.1080/15592324.2024.2446858","DOIUrl":"https://doi.org/10.1080/15592324.2024.2446858","url":null,"abstract":"<p><p>Reproducibility in organ size and shape is a fascinating trait of living organisms. The mechanisms underlying such robustness remain, however, to be elucidated. Taking the sepal of Arabidopsis as a model, we investigated whether variability of gene expression plays a role in variation of organ size and shape. Previous work from our team identified cell-wall related genes as being enriched among the genes whose expression is highly variable. We then hypothesized that the variation of measured morphological parameters in cell-wall related single knockout mutants could be correlated with the variation in gene expression of the corresponding gene (the knocked-out gene) in wild-type plants. We analyzed sepal size and shape from 16 cell-wall mutants and found that sepal size variability correlates positively, not with gene expression variation, but with mean gene expression of the corresponding gene in wild type. These findings support a contribution of cell-wall related genes to the robustness of sepal size.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"20 1","pages":"2446858"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142911205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression characteristics of <i>CsESA1</i> in citrus and analysis of its interacting protein.","authors":"Xiao He, Huiying Wang, Wei Wei, Ziyue Han, Jiaqi Zuo, Qing He","doi":"10.1080/15592324.2024.2439249","DOIUrl":"10.1080/15592324.2024.2439249","url":null,"abstract":"<p><p>The most damaging disease affecting citrus globally is Huanglongbing (HLB), primarily attributed to the infection by '<i>Candidatus Liberibacter</i> asiaticus' (<i>Ca</i>Las). Based on comparative transcriptome data, two cellulose synthase (CESA) genes responsive to <i>Ca</i>Las infection induction were screened, and one gene cloned with higher differential expression level was selected and named <i>CsCESA1</i>. we verified the interaction between CsCESA1 and citrus exopolysaccharide 2 (CsEPS2) proteins. Subcellular localization in tobacco indicated that both CsCESA1 and CsEPS2 proteins are primarily located in the nucleus and cytoplasm. RT-qPCR analysis indicated that the expression levels of <i>CsCESA1</i> and <i>CsEPS2</i> were associated with variety tolerance, tissue site, and symptom development. Furthermore, we generated <i>CsCESA1</i> and <i>CsEPS2</i> silencing plants and obtained <i>CsCESA1</i> and <i>CsEPS2</i> silencing and overexpressing hairy roots. The analysis of hormone content and gene expression also showed that <i>CsCESA1</i> and <i>CsEPS2</i> are involved in transcriptional regulation of genes involved in systemic acquired resistance (SAR) response. In conclusion, our results suggested that <i>CsCESA1</i> and <i>CsEPS2</i> could serve as potential resistance genes for HLB disease, offering insights into the plant's defense mechanisms against HLB.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"20 1","pages":"2439249"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142879434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative effects of biocontrol agent and pathogen on <i>Nicotiana tabacum</i>: insights into fungal-plant interactions.","authors":"Quanyu Yin, Zhichao Ren, Dongling Wu, Zhao Feng, Zhengkang Zhu, Amit Jaisi, Hui Wang, Mengquan Yang","doi":"10.1080/15592324.2025.2453562","DOIUrl":"https://doi.org/10.1080/15592324.2025.2453562","url":null,"abstract":"<p><p>Tobacco (<i>Nicotiana tabacum</i>) black shank disease, caused by <i>Phytophthora nicotianae</i>, is a significant threat to tobacco crops, leading to severe economic losses. Prolonged use of agrochemicals to control this disease has prompted the exploration of eco-friendly biological control strategies. This study investigated the effects of <i>Trichoderma harzianum</i>, a biocontrol agent, on <i>N. tabacum</i> in comparison to <i>P. nicotianae</i>, focusing on growth, biomass, root morphology and anatomy, hormonal changes, and osmotic regulation. <i>T. harzianum</i> significantly enhanced plant growth, biomass accumulation, root system development, and physiological attributes such as photosynthetic pigment levels and antioxidant enzyme activity. In contrast, <i>P. nicotianae</i> negatively impacted these parameters, inhibiting growth and physiological function. Notably, <i>T. harzianum</i> increased proline content and enhanced induced resistance mechanisms, mitigating stress and promoting overall plant health. These findings highlight the potential of <i>T. harzianum</i> as a sustainable solution for managing black shank disease while improving tobacco crop productivity.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"20 1","pages":"2453562"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143056175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aba-induced active stomatal closure in bulb scales of Lanzhou lily.","authors":"Lei Gong, Hai-Qing Liu, Ye Hua, Ya-Yun Zhang, Md Mahadi Hasan","doi":"10.1080/15592324.2024.2446865","DOIUrl":"https://doi.org/10.1080/15592324.2024.2446865","url":null,"abstract":"<p><p>Abscisic acid (ABA) mediated stomatal closure is a highly effective mode of active stomatal regulation under drought stress. Previous studies on stomatal regulation have primarily focused on the leaves of vascular plants, while research on the stomatal behavior of bulbous plants remains unknown. In addition, ABA-induced stomatal regulation in bulbs has yet to be explored. Therefore, we aim to investigate the ABA-induced active regulation in the bulb of the Lanzhou lily (<i>Lilium davidii</i> var. unicolor). The morphological characteristics of epidermal strips were analyzed along with a stomatal aperture assay to investigate the bulb's stomatal response to ABA. Moreover, the mechanism of ABA signaling was explored using treatments with ABA signaling chemicals and corresponding scavengers. This study revealed that stomata are mainly distributed on the upper part and outer surface of the bulb. The guard cells of the lily bulb are inflated, and the stomata have a nearly circular shape with relatively low stomatal density. Exogenous ABA was found to induce varying degrees of stomatal closure in a dose-dependent manner, with significant stomatal aperture reduction observed after treatment with 10 µM ABA. Overall, the study indicated that both hydrogen peroxide (H₂O₂) and nitric oxide (NO) are involved in the ABA-induced stomatal closure process, with H₂O₂ functioning as an upstream component of NO.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"20 1","pages":"2446865"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142901551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}