Carbohydrate Research最新文献_第3页

α-lipoic acid and L-serine conjugated chitosan as a stimuli-responsive drug delivery platform α-硫辛酸-l -丝氨酸偶联壳聚糖作为刺激反应性给药平台的研究

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2026-04-01 Epub Date: 2026-01-21 DOI: 10.1016/j.carres.2026.109837

Özlem Kaplan , Kevser Bal , Sibel Küçükertuğrul Çeli̇k , Nazan Gökşen Tosun , Mehmet Koray Gök

{"title":"α-lipoic acid and L-serine conjugated chitosan as a stimuli-responsive drug delivery platform","authors":"Özlem Kaplan , Kevser Bal , Sibel Küçükertuğrul Çeli̇k , Nazan Gökşen Tosun , Mehmet Koray Gök","doi":"10.1016/j.carres.2026.109837","DOIUrl":"10.1016/j.carres.2026.109837","url":null,"abstract":"<div><div>In this study, a novel chitosan-based nanocarrier system was developed through dual modification with α-lipoic acid and <span>l</span>-serine (Chi<sub>Lipo–Ser</sub>) to enhance its physicochemical and biological properties for drug delivery applications. FTIR and <sup>1</sup>H NMR spectroscopy verified the successful conjugation of functional groups onto the chitosan backbone, while GPC analysis confirmed an increase in molecular weight consistent with the modification. Compared with native chitosan, the Chi<sub>Lipo–Ser</sub> exhibited improved solubility at physiological pH and enhanced buffering capacity. Nanoparticles prepared via ionotropic gelation demonstrated favorable characteristics, including an average particle size of ∼150 nm, low polydispersity, and a positive surface charge. Redox sensitivity was evidenced by DLS analysis, which revealed particle destabilization in the presence of 10–20 mM DTT, resulting from disulfide bond cleavage. Importantly, Chi<sub>Lipo–Ser</sub> modification significantly enhanced the encapsulation efficiency of curcumin compared to unmodified chitosan, while the resulting formulations also exhibited effective release behavior at pH 5, mimicking the tumor intracellular environment. Furthermore, <em>in vitro</em> cytotoxicity assays showed that CUR-loaded Chi<sub>Lipo–Ser</sub> nanoparticles exerted cytotoxic effects against HeLa and HT29 cancer cells while maintaining acceptable biocompatibility in BJ fibroblasts. Collectively, these results highlight the potential of Chi<sub>Lipo–Ser</sub> nanoparticles as a promising platform for stimuli-responsive anticancer drug delivery.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"562 ","pages":"Article 109837"},"PeriodicalIF":2.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146036015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Only in Halobacterium salinarum: Sugar modifications unique to an archaeal N-linked glycan 仅在盐盐菌中：古细菌n链聚糖特有的糖修饰

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2026-04-01 Epub Date: 2026-01-12 DOI: 10.1016/j.carres.2026.109817

Jerry Eichler, Zlata Vershinin, Marianna Zaretsky

{"title":"Only in Halobacterium salinarum: Sugar modifications unique to an archaeal N-linked glycan","authors":"Jerry Eichler, Zlata Vershinin, Marianna Zaretsky","doi":"10.1016/j.carres.2026.109817","DOIUrl":"10.1016/j.carres.2026.109817","url":null,"abstract":"<div><div><em>Halobacterium salinarum</em> are halophilic archaea that grow at or near saturating salt conditions. In addition to providing insight into how life copes with extreme conditions, studying <em>Hbt. salinarum</em> has been central to advances made in numerous fields, including bioenergetics, membrane protein structure determination and optogenetics. From the perspective of carbohydrate research, <em>Hbt. salinarum</em> provided the first example of N-glycosylation outside Eukarya. Yet, even 50 years after the first report of such post-translational modification in these haloarchaea, various aspects of <em>Hbt. salinarum</em> N-glycosylation seemingly unique to this organism remain largely unaddressed. These include questions related to the incorporation of iduronic acid in an N-linked glycan decorating <em>Hbt. salinarum</em> glycoproteins and the sulfation of this sugar at the O-3 position, as well as the transient methylation of this glycan at the lipid carrier- but not the protein-bound stage. In this review, recent progress on each of these unusual and unique aspects of <em>Hbt. salinarum</em> N-glycosylation is discussed.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"562 ","pages":"Article 109817"},"PeriodicalIF":2.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145976368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extraction, purification, structure characteristics and biological activities of polysaccharides from Saccharina japonica (Laminaria japonica): A review 海带多糖的提取纯化、结构特征及生物活性研究进展。

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2026-04-01 Epub Date: 2026-01-13 DOI: 10.1016/j.carres.2026.109826

Feiya Zhao , Mingyang Cao , Bei Wang , Shineng Jiang , Jing Li , Tao Aien

{"title":"Extraction, purification, structure characteristics and biological activities of polysaccharides from Saccharina japonica (Laminaria japonica): A review","authors":"Feiya Zhao , Mingyang Cao , Bei Wang , Shineng Jiang , Jing Li , Tao Aien","doi":"10.1016/j.carres.2026.109826","DOIUrl":"10.1016/j.carres.2026.109826","url":null,"abstract":"<div><div><em>Saccharina japonica</em> (formerly known as <em>Laminaria japonica</em>) is a valuable marine resource with a triple significance: it serves not only as a food source but also as a component in traditional medicine, while simultaneously playing an important ecological role in marine environments. Among its key chemical constituents, <em>Saccharina japonica</em> polysaccharides (SJPs; <em>Laminaria japonica</em> polysaccharides, LJPs) exhibit a range of notable biological activities, including immunomodulatory, antioxidant, and antitumor effects, as well as the ability to modulate gut microbiota. Contemporary research on SJPs aims to elucidate their biological mechanisms, thereby validating and scientifically explaining the molecular basis of their traditional therapeutic effects. This effort not only deepens our understanding of ethnopharmacological knowledge but also provides critical scientific evidence for their application in high-value domains such as functional foods, nutraceuticals, and novel pharmaceutical adjuvants. This review summarizes current extraction and purification methods for SJPs, discusses their biological activities with emphasis on structure-activity relationships, and explores their potential applications in functional foods, biomedicine, and cosmetics, thereby laying a theoretical foundation for further research and product development.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"562 ","pages":"Article 109826"},"PeriodicalIF":2.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146008125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methyl 2-deoxy-3,5-di-O-p-toluoyl-α-d-ribofuranoside: isolation, crystal structure and conformation 甲基2-脱氧-3,5-二- o -对甲苯基-α-d-核呋喃苷：分离、晶体结构和构象。

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2026-04-01 Epub Date: 2026-01-16 DOI: 10.1016/j.carres.2026.109831

Eleanor Dodd , Simon J. Coles , William Fraser

引用次数: 0

Synthesis of glycosyl chlorides and fluorides via a mild chlorination strategy 通过温和氯化策略合成糖基氯化物和氟化物

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2026-04-01 Epub Date: 2026-01-28 DOI: 10.1016/j.carres.2026.109841

Soumyadip Dey, Adrita Nandy, Shubhi Dwivedi, Abhijit Sau

引用次数: 0

Anomeric effect in six-membered rings: An NBO perspective 六元环的反常效应：一个NBO的视角

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2026-04-01 Epub Date: 2026-01-28 DOI: 10.1016/j.carres.2026.109840

Matheus P. Freitas

{"title":"Anomeric effect in six-membered rings: An NBO perspective","authors":"Matheus P. Freitas","doi":"10.1016/j.carres.2026.109840","DOIUrl":"10.1016/j.carres.2026.109840","url":null,"abstract":"<div><div>The anomeric effect arises from a subtle balance between steric, electrostatic, and orbital interactions, yet its relative energetic origins remain debated. Here, we present a systematic computational study of substituted cyclohexanes, tetrahydropyrans, and thianes (Y<img>CH<sub>3</sub>, CF<sub>3</sub>, F, and Cl) to disentangle these contributions. Conformational energies were evaluated in the gas phase and in implicit water, and natural bond orbital (NBO) analyses were employed to decompose steric exchange, electrostatic (NCE), and electron-delocalization effects. While cyclohexane derivatives largely follow steric expectations, heterocycles containing oxygen or sulfur display pronounced axial stabilization for electronegative substituents, consistent with the anomeric effect. This stabilization is dominated by <em>n</em><sub>X</sub> → σ∗<sub>C</sub>–<sub>Y</sub> hyperconjugation and supported by favorable electrostatics, whereas steric effects act mainly as a modulating factor and appear underestimated within the present partitioning scheme. Importantly, the qualitative balance between electrostatic and hyperconjugative contributions persists in polar solution, indicating that intramolecular dipolar interactions remain operative even under high-dielectric conditions.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"562 ","pages":"Article 109840"},"PeriodicalIF":2.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146074863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural studies on cell wall and matrix polysaccharides of Micrococcus luteus HB3.2.1 黄体微球菌HB3.2.1细胞壁及基质多糖的结构研究

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2026-04-01 Epub Date: 2026-01-08 DOI: 10.1016/j.carres.2026.109814

Evelina L. Zdorovenko , Ecaterina V. Diuvenji , Svetlana V. Zhenilo , Ekaterina D. Nevolina , Andrey S. Dmitrenok , Sergey V. Martyanov , Alexander S. Shashkov , Artem G. Chebotarevskii , Egor B. Prokhorchuk , Vladimir K. Plakunov , Andrei V. Gannesen

{"title":"Structural studies on cell wall and matrix polysaccharides of Micrococcus luteus HB3.2.1","authors":"Evelina L. Zdorovenko , Ecaterina V. Diuvenji , Svetlana V. Zhenilo , Ekaterina D. Nevolina , Andrey S. Dmitrenok , Sergey V. Martyanov , Alexander S. Shashkov , Artem G. Chebotarevskii , Egor B. Prokhorchuk , Vladimir K. Plakunov , Andrei V. Gannesen","doi":"10.1016/j.carres.2026.109814","DOIUrl":"10.1016/j.carres.2026.109814","url":null,"abstract":"<div><div>Matrix and cell wall polysaccharides of <em>Micrococcus luteus</em> HB3.2.1 were obtained. The following structures of the glycopolymers were established by compositional analysis and 1D and 2D NMR spectroscopy:</div><div>→4)-ɑ-<span>d</span>-Gal<em>p</em>NAc-(1→2)-<span>l</span>-2HO-Glt-(5→2)-<span>d</span>-Asn-(1→4)-ɑ-<span>d</span>-Qui<em>p</em>NAc4N-(1→ PSI.</div><div>where <span>d</span>-Asn indicates <span>d</span>-asparagine, <span>l</span>-2HO-Glt – <span>l</span>-2-hydroxy-glutaric acid.</div><div>→2)-ɑ-<span>d</span>-Man<em>p</em>-(1→3)-ɑ-<span>d</span>-Man<em>p</em>-(1→6)-ɑ-<span>d</span>-Man<em>p</em>-(1→ PSII.</div><div>→6)-ɑ-<span>d</span>-Glc<em>p</em>-(1→4)-β-<span>d</span>-Man<em>p</em>NAcA-(1→ PSIII.</div><div>→6)-ɑ-<span>d</span>-Glc<em>p</em>-(1→4)-β-<span>d</span>-Glc<em>p</em>NAc3NAcA-(1→ PSIV.</div><div>The structure of the PSI is unique among the bacterial polysaccharides. It is including non-sugar residues such as <span>d</span>-asparagine and<span>l</span>-2-hydroxy-glutaric acid in the main chain.</div><div>The matrix polysaccharide (PSI) possesses a cytotoxic activity.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"562 ","pages":"Article 109814"},"PeriodicalIF":2.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145950155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural fragments of an immunomodulatory active exopolysaccharide produced by the green microalgae Dictyosphaerium tetrachotomum Fott 绿色微藻Dictyosphaerium tetrachotomum Fott产生的免疫调节活性外多糖的结构片段。

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2026-04-01 Epub Date: 2026-01-16 DOI: 10.1016/j.carres.2026.109834

Peter Capek, Mária Matulová, Iveta Uhliariková

{"title":"Structural fragments of an immunomodulatory active exopolysaccharide produced by the green microalgae Dictyosphaerium tetrachotomum Fott","authors":"Peter Capek, Mária Matulová, Iveta Uhliariková","doi":"10.1016/j.carres.2026.109834","DOIUrl":"10.1016/j.carres.2026.109834","url":null,"abstract":"<div><div>Microalgal metabolites represent source of valuable compounds with wide range of biological effects and industrial applications. Eight sugar units found in more than twenty methylated derivatives, of which Gal, Glc, Fuc, Xyl and GlcA were dominant, suggest complex structure of <em>D. tetrachotomum</em> exopolysaccharide (DtF). Almost 20 % of Glc is partially methylated as 2-OMe-Glc. Ten fractions obtained by ion-exchange chromatography were analysed by chemical and spectroscopic methods. Fractions DtF1, DtF4 and DtF5 were isolated in the highest yields. The lower molecular weight DtF1 biopolymer was identified as the neutral β-D-<em>gluco</em>-3,6-β-D-galactan with fragments of unsubstituted 1,3-linked βGalp alternating 1,3,6-linked βGalp branched at O3 by <em>O</em>Me and βGlcp. The high molecular weight fractions DtF4 and DtF5 were rich in Gal, Glc, Fuc, Xyl and GlcA residues. Methylation results support 2,4-fuco-4-β-D-galactan backbone terminated by Glc, Xyl and GlcA residues. The results indicate at least two types of polymers in DtF; a minor neutral β-D-<em>gluco</em>-3,6-β-D-galactan and a dominant acidic one with a 2,4-fuco-4-β-D-galactan backbone. Partial acid hydrolyses of DtF, DtF4 and DtF5 led to identification of oligomers. Each one has at the reducing end Fuc substituted at O2 by 2-<em>O</em>Me-αGlc and at O4 by substituents R = βGlcpA; βGalp; βGalp (1 → 3)βGalp (1→ and Xylp (1 → 4)βGalp (1 → 3)βGalp (1 → .</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"562 ","pages":"Article 109834"},"PeriodicalIF":2.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146028531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A rapid, high-throughput assay for the detection of O-GlcNAcylation 一种快速、高通量的o - glcn酰化检测方法。

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2026-04-01 Epub Date: 2026-01-12 DOI: 10.1016/j.carres.2026.109818

Xiaoyu Yang , Huanhuan Zhang , Chunhua Yang, Yafang Bai, Zhanyi Yang, Jia Mi, Yanping Zhu

{"title":"A rapid, high-throughput assay for the detection of O-GlcNAcylation","authors":"Xiaoyu Yang , Huanhuan Zhang , Chunhua Yang, Yafang Bai, Zhanyi Yang, Jia Mi, Yanping Zhu","doi":"10.1016/j.carres.2026.109818","DOIUrl":"10.1016/j.carres.2026.109818","url":null,"abstract":"<div><div>The level of O-GlcNAcylation is a critical sensor of cellular metabolic status. However, high-throughput quantification of protein O-GlcNAcylation remains a great challenge owing to its low abundance and labile nature. In this study, we developed a Quantitative Dot Blot (QDB)-based assay for quantifying the global O-GlcNAcylation of proteins in complex biological samples. For the detection of O-GlcNAcylation proteins, the QDB method exhibited high sensitivity, yielding a linear response over the range of 2.5–20 μg of total protein with a coefficient of determination (R<sup>2</sup>) of 0.99, and the limit of detection was determined to be 2.5 μg. And the QDB assay exhibited robust performance in quantifying variable O-GlcNAcylation levels in TMG-treated HEK293T cells, with a linear regression R<sup>2</sup> of 0.9353. We further validated the applicability of the QDB method in tissues from diabetic mice, including the soleus muscle and retina. The trends in O-GlcNAcylation changes detected by QDB were consistent with those obtained via Western blot (WB). Collectively, these data demonstrate that the QDB assay can be applied to measure O-GlcNAcylation levels in diverse tissue types. It may thus serve as an effective tool for evaluating O-GlcNAcylation activity and inhibitory effects in clinical diagnostics. Moreover, the QDB platform could potentially be extended to the detection and monitoring of other post-translational modifications.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"562 ","pages":"Article 109818"},"PeriodicalIF":2.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146009219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive preparation of uronic and aldaric acids by bacteria possessing aldose C-6 alcohol dehydrogenase activity 具有醛糖C-6醇脱氢酶活性的细菌综合制备醛酸和醛酸。

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2026-04-01 Epub Date: 2026-01-27 DOI: 10.1016/j.carres.2026.109839

Takaaki Kiryu , Koki Fujita , Motohiro Shizuma , Hiroaki Tatsuoka

{"title":"Comprehensive preparation of uronic and aldaric acids by bacteria possessing aldose C-6 alcohol dehydrogenase activity","authors":"Takaaki Kiryu , Koki Fujita , Motohiro Shizuma , Hiroaki Tatsuoka","doi":"10.1016/j.carres.2026.109839","DOIUrl":"10.1016/j.carres.2026.109839","url":null,"abstract":"<div><div>Sixteen types of uronic acids are derived from aldohexoses. Except for a few examples, uronic acids are difficult to prepare and are not commercially available as reagents. Our previous study reported that C-6 oxidation of aldose (<span>d</span>-glucose) and aldonic acid (<span>d</span>-gluconic acid) by alcohol dehydrogenases from <em>Pseudogluconobacter saccharoketogenes</em> Rh47-3 produced the uronic acids (<span>d</span>-glucuronic acid and <span>l</span>-guluronic acid, respectively). Aldaric acid (<span>d</span>-glucaric acid) was also produced by further oxidation with aldehyde dehydrogenase from the same bacterium. The present study demonstrates that alcohol dehydrogenase widely oxidizes aldoses and aldonic acids, while aldehyde dehydrogenase extensively oxidizes aldoses and uronic acids; the alcohol dehydrogenase was named “aldose C-6 alcohol dehydrogenase” Except for commercially available <span>d</span>- and <span>l</span>-gulonic acid, all aldonic acids (14 types), uronic acids (16 types), and aldaric acids (10 types) derived from aldohexoses could be prepared using the alcohol dehydrogenase and resting cells. These sugar acids were obtained by oxidizing various aldoses and aldonic acids through either aldose C-1/C-6-inverting oxidation or aldose C-1/C-6-retaining oxidation, although the production of <span>d</span>-iduronic acid and <span>d</span>-idaric acid was low. The same reaction was also performed with <em>Youhaiella</em> spp., a registered strain in the Japan Collection of Microorganisms. The results provide comprehensive and simple methods for preparing sugar acids.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"562 ","pages":"Article 109839"},"PeriodicalIF":2.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146104086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0