羊肚菌子实体中一种新的多糖：化学结构和免疫调节能力

IF 2.4 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research Pub Date : 2025-05-15 DOI:10.1016/j.carres.2025.109529

Gopal De , Debsankar Das , Andreas Koschella , Sanhita Ghosh , Chiranjib Pal

{"title":"羊肚菌子实体中一种新的多糖：化学结构和免疫调节能力","authors":"Gopal De , Debsankar Das , Andreas Koschella , Sanhita Ghosh , Chiranjib Pal","doi":"10.1016/j.carres.2025.109529","DOIUrl":null,"url":null,"abstract":"<div><div>A new polysaccharide (MEPS, Morchella elata polysaccharide) obtained through alkali extraction from fruit bodies of Morchella elata was purified by dialysis and sepharose-6B column chromatography. Chemical and spectroscopic analysis including GLC, GLC-MS, FT-IR, 1D NMR (1H, 13C, DEPT-135) and 2D NMR (TOCSY, HMQC, NOESY, DQF, ROESY, and HMBC) revealed that the backbone of its repeating unit was →6)-β-d-Manp-(1 → 3)-α-d-Glcp-(1 → 4)-α-d-Glcp-(1→, branched at C-3 of (1 → 4)-linked- α-d-Glcp by α-d-Manp-(1 → 4)-α-d-Glcp-(1 → 4)-α-d-Galp-(1 → . It was also found that a terminal β-l-fucopyranosyl residue was attached at C-3 of α-d-glucopyranosyl residue of the branched chain. In vitro evaluation of immune modulation of the polysaccharide revealed the significant macrophage polarizing properties and capability to induce an alternative phenotype M2 in peritoneal macrophages obtained from BALB/c mice. It was further confirmed by the ability to induce the expression of M2 polarizing cytokines, specifically IL-10.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"554 ","pages":"Article 109529"},"PeriodicalIF":2.4000,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A new polysaccharide from fruit bodies of Morchella elata: Chemical structure and immunomodulation abilities\",\"authors\":\"Gopal De , Debsankar Das , Andreas Koschella , Sanhita Ghosh , Chiranjib Pal\",\"doi\":\"10.1016/j.carres.2025.109529\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>A new polysaccharide (MEPS, Morchella elata polysaccharide) obtained through alkali extraction from fruit bodies of Morchella elata was purified by dialysis and sepharose-6B column chromatography. Chemical and spectroscopic analysis including GLC, GLC-MS, FT-IR, 1D NMR (1H, 13C, DEPT-135) and 2D NMR (TOCSY, HMQC, NOESY, DQF, ROESY, and HMBC) revealed that the backbone of its repeating unit was →6)-β-d-Manp-(1 → 3)-α-d-Glcp-(1 → 4)-α-d-Glcp-(1→, branched at C-3 of (1 → 4)-linked- α-d-Glcp by α-d-Manp-(1 → 4)-α-d-Glcp-(1 → 4)-α-d-Galp-(1 → . It was also found that a terminal β-l-fucopyranosyl residue was attached at C-3 of α-d-glucopyranosyl residue of the branched chain. In vitro evaluation of immune modulation of the polysaccharide revealed the significant macrophage polarizing properties and capability to induce an alternative phenotype M2 in peritoneal macrophages obtained from BALB/c mice. It was further confirmed by the ability to induce the expression of M2 polarizing cytokines, specifically IL-10.</div></div>\",\"PeriodicalId\":9415,\"journal\":{\"name\":\"Carbohydrate Research\",\"volume\":\"554 \",\"pages\":\"Article 109529\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2025-05-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Carbohydrate Research\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0008621525001557\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Carbohydrate Research","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0008621525001557","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

以羊肚菌（Morchella elata）的子实体为原料，经透析和蔗糖- 6b柱层析纯化得到一种新的羊肚菌多糖MEPS。GLC、GLC- ms、FT-IR、1D NMR （1H、13C、DEPT-135）和2D NMR （TOCSY、HMQC、NOESY、DQF、ROESY和HMBC）的化学和光谱分析表明，其重复单元的主链为→6)-β-d- manp -(1→3)-α-d-Glcp-(1→4)-α-d-Glcp-(1→4)-α-d-Glcp-(1→4)-α-d- galp -(1→4)-(1→4)-α-d-Glcp-(1→4)-α-d- galp -（1→）在（1→4）-连接-α-d-Glcp的C-3处支链。还发现在支链α-d-葡萄糖吡喃基残基的C-3处有末端β-l-fucopyranosyl残基。体外免疫调节评价表明，该多糖在BALB/c小鼠腹腔巨噬细胞中具有显著的巨噬细胞极化特性和诱导替代表型M2的能力。通过诱导M2极化细胞因子，特别是IL-10的表达，进一步证实了这一点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

A new polysaccharide from fruit bodies of Morchella elata: Chemical structure and immunomodulation abilities

A new polysaccharide (MEPS, Morchella elata polysaccharide) obtained through alkali extraction from fruit bodies of Morchella elata was purified by dialysis and sepharose-6B column chromatography. Chemical and spectroscopic analysis including GLC, GLC-MS, FT-IR, 1D NMR (¹H, ¹³C, DEPT-135) and 2D NMR (TOCSY, HMQC, NOESY, DQF, ROESY, and HMBC) revealed that the backbone of its repeating unit was →6)-β-d-Manp-(1 → 3)-α-d-Glcp-(1 → 4)-α-d-Glcp-(1→, branched at C-3 of (1 → 4)-linked- α-d-Glcp by α-d-Manp-(1 → 4)-α-d-Glcp-(1 → 4)-α-d-Galp-(1 → . It was also found that a terminal β-l-fucopyranosyl residue was attached at C-3 of α-d-glucopyranosyl residue of the branched chain. In vitro evaluation of immune modulation of the polysaccharide revealed the significant macrophage polarizing properties and capability to induce an alternative phenotype M2 in peritoneal macrophages obtained from BALB/c mice. It was further confirmed by the ability to induce the expression of M2 polarizing cytokines, specifically IL-10.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Carbohydrate Research 化学-生化与分子生物学

CiteScore

5.00

自引率

3.20%

发文量

183

审稿时长

3.6 weeks

期刊介绍： Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".