Carbohydrate Research最新文献

{"title":"Isolation, structural characterization of (1 → 4), (1 → 6)-α-D-glucan from Dioscorea Alata and synthesis and catalytic activity studies of (1 → 4), (1 → 6)-α-D-glucan derived carbon dots.","authors":"Arnab Mallik, Sukesh Patra, Animesh Patra, Akhil Pandey, Madhumita Hazra, Gobinda Prasad Sahoo","doi":"10.1016/j.carres.2025.109383","DOIUrl":"10.1016/j.carres.2025.109383","url":null,"abstract":"<p><p>Herein, a straightforward, productive protocol was adopted for the synthesis of carbon dots (CDs) by a simple microwave-assisted technique from (1 → 4), (1 → 6)-α-D-glucan polysaccharide (DAPS). The isolation and structural characterization of (1 → 4), (1 → 6)-α-D-glucan from the aqueous extracting of the Diascorea Alata was described here. The photo-physical and morphological studies of the prepared high quantum yield (27.70 %) CDs were systematically characterized using different analytical techniques: TEM, DLS, XPS, XRD, FT-IR, TCSPC, EDX, Fluorescence and UV-vis spectroscopy. In our study the carbon dots (CDs) obtained are non-toxic, highly water-soluble, spherical-shaped negatively charged particles with an average diameter of 3.28 nm. The photo-catalytic activity of carbon dots (CDs) was tested under the sunlight for photo-degradation of crystal violet (CV), a pollutant organic dye. Carbon dots (CDs) showed outstanding results regarding the photo degradation of CV (achieving 99.9 % degradation within 8 min) under sunlight.</p>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"549 ","pages":"109383"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structure characterization and preliminary immune activity of a glucomannan purified from Allii Tuberosi Semen.","authors":"Huiwen Li, Aigen Fu, Heping Hui, Feng Jia, Huan Wang, Tong Zhao, Jing Wei, Peijuan Zhang, Wuying Lang, Kejuan Li, Xuansheng Hu","doi":"10.1016/j.carres.2025.109375","DOIUrl":"https://doi.org/10.1016/j.carres.2025.109375","url":null,"abstract":"<p><p>A novel glucomannan, named ATSW-1, was obtained from Allii Tuberosi Semen by ion and gel permeation chromatography purification. Its structure was characterized using high-performance chromatography, Fourier transform infrared spectroscopy, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy. The in vitro immune activities of ATSW-1 were examined using the Cell Counting Kit-8, the neutral red phagocytosis assay, and the secretion of related cytokines. The results showed that the molecular weight of ATSW-1 was 35.9 kDa and mainly constituted of mannose, glucose, and arabinose with a molar ratio of 1:0.72:0.04. Its main backbone was composed of →4)-β-D-Glcp-(1→, →4)-β-D-Manp-(1→, and →4,6)-α-D-Manp-(1→, with the branches β-D-Glcp-(1→, α-L-Araf-(1→ and →5)-α-L-Araf-(1→ linkage substituted at C-6 position of →4)-α-D-Manp-(1 → . ATSW-1 significantly enhanced immune responses, increasing the phagocytic activity of RAW264.7 cells by 1.52 times compared to the blank control group. Further investigations revealed that ATSW-1 promotes immune activity by upregulating the NO, TNF-α, and IL-6 secretion. These findings provide a foundation for the potential development and application of Allii Tuberosi Semen polysaccharides in functional foods or immune-related therapeutics.</p>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"549 ","pages":"109375"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drug release potential of polyacrylamide grafted-Assam Bora rice polysaccharide graft copolymer (ABRS-g-PAM) as effective controlled release polymer.","authors":"Neha Singh, Itishree Jogamaya Das, Sutapa Satpathi, Devjit Das, Bhabani Shankar Patro, Karmabeer Jena, Subhendu Chakroborty, Pankaj Dagar, Trishna Bal","doi":"10.1016/j.carres.2024.109355","DOIUrl":"10.1016/j.carres.2024.109355","url":null,"abstract":"<p><p>The research focuses on the characterization and evaluation of drug delivery efficiency of a microwave-assisted, free-radical synthesized polyacrylamide-grafted Assam Bora rice starch (ABRS) graft copolymer (ABRS-g-PAM). Percentage grafting efficiency (% GE) and intrinsic viscosity were chosen as the optimization parameters. The optimized ABRS-g-PAM Grade Formulation 4 (GF4) was found to be the best grade. GF4 was characterized through XRD, FTIR, TGA, DSC, and ¹³C NMR, confirming efficient polyacrylamide grafting onto ABRS, besides swelling studies. SEM and FESEM showed a rough GF4 surface. Further analysis using Atomic Force Microscopy (AFM) detailed the irregular, rough internal architecture. The maximum swelling of GF4 occurred at pH 7 and 0.005 (M) NaCl, demonstrating second-order swelling kinetics. The soil biodegradability study of GF4 confirmed its biocompatibility. In vitro drug release studies showed that GF4 achieved 99 % release of Rosuvastatin over 26 h, thereby supporting the controlled release behaviour of the optimized polymer.</p>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"549 ","pages":"109355"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cancer cell adhesion property on all-chitin composite films with reduced crystallinity.","authors":"Masayasu Totani, Hiroyuki Shinchi, Jun-Ichi Kadokawa","doi":"10.1016/j.carres.2024.109373","DOIUrl":"10.1016/j.carres.2024.109373","url":null,"abstract":"<p><p>We previously prepared self-reinforced chitin composite (SR-ChC) films, also called all-chitin composites, comprising two components, that is, scale-down chitin nanofibers (SD-ChNFs) with high crystallinity and scale-down low-crystalline chitin (SD-LC-Ch) matrixes. In this study, we precisely evaluated hydrophilicity under water enviromental conditions and its effect on cell adhesion using human-derived cancer cells on the SR-ChC film surfaces. The surface analysis of the SR-ChC films with reduced crystallinity revealed reorientation of the molecular chain assemblies with amino groups in the SD-LC-Ch components in water. Consequently, the amount of the SD-LC-Ch components gave rise to increasing the number of hydrophilic amino groups on the film surfaces, which resulted in efficiency of cell adhesion and elongation. This study concludes that the hydrophilic SD-LC-Ch components in the lower crystalline SR-ChC films strongly contribute to exhibiting new functions, related to interaction with biological substrates, such as cells. © 2017 ElsevierInc.Allrightsreserved.</p>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"549 ","pages":"109373"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142929964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Block synthesis of Forssman pentasaccharide GalNAcα1-3GalNAcβ1-3Galα1-4Galβ1-4Glcβ in the form of 3-aminopropyl glycoside.","authors":"Inna S Popova, Marina A Sablina, Ivan M Ryzhov, Darya O Anisimova, Tatiana V Ovchinnikova, Tatiana V Tyrtysh, Elena Yu Korchagina, Alexander O Chizhov, Alexander S Paramonov, Nicolai V Bovin","doi":"10.1016/j.carres.2024.109370","DOIUrl":"10.1016/j.carres.2024.109370","url":null,"abstract":"<p><p>A total chemical synthesis of spacer-armed Forssman pentasaccharide is reported. The choice of the 2(donor) + 3(acceptor) block scheme, the optimal combination of a limited number of simple protecting groups and the sequence of deprotection steps allowed to achieve the high yield and stereoselectivity of glycosylation and to avoid losses during deprotection. The target pentasaccharide was obtained in a 10-mg scale. ¹H and ¹³C NMR spectra of the Forssman pentasaccharide were completely assigned with the use of various 2D-NMR experiments.</p>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"549 ","pages":"109370"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142963764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in structural modification of fucoidans, ulvans, and carrageenans to improve their biological functions for potential therapeutic application.","authors":"Sara El Asri, Reda Ben Mrid, Zakia Zouaoui, Zoulfa Roussi, Abdelhamid Ennoury, Mohamed Nhiri, Fatiha Chibi","doi":"10.1016/j.carres.2024.109358","DOIUrl":"10.1016/j.carres.2024.109358","url":null,"abstract":"<p><p>Marine sulfated polysaccharides constitute a class of bioactive polymers commonly found in cell walls of macroalgae. Among these macromolecular substances, fucoidans, ulvans, and carrageenans have attracted considerable attention providing interesting therapeutic properties affected by a combination of various structural factors, such as sulfation pattern, molecular weight, monosaccharide composition, and glycosidic linkages. Remarkably, chemical modification, enzymatic hydrolysis and crosslinking are promising approaches for developing the application of these polysaccharides through enhancement and/or addition of new biological properties. This paper reviews the recent advances on these structure modification methods on fucoidans, ulvans, and carrageenans. The physical, chemical and biological properties influenced by the addition of functional groups are also discussed. In addition, an overview of specific enzymes selectively producing oligosaccharides with improved bioactivities as well as ionic and covalent cross-linking strategies are provided. These targeted methods have the potential to develop novel compounds with outstanding biodegradability and biocompatibility, along with low toxicity suitable for diverse applications in biomedical fields, including drug delivery.</p>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"549 ","pages":"109358"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of branched heterooligosaccharides related to Aspergillus galactomannan containing short Galf side chains.","authors":"Darya A Rastrepaeva, Dmitry A Argunov, Ilya A Puchkin, Dmitry V Yashunsky, Vadim B Krylov, Nikolay E Nifantiev","doi":"10.1016/j.carres.2024.109360","DOIUrl":"10.1016/j.carres.2024.109360","url":null,"abstract":"<p><p>The members of a widespread Aspergillus fungi genus cause various diseases including the invasive aspergillosis with high morbidity and mortality rates, especially for immunosuppressed patients. One of the main carbohydrate structures on the surface of their cell wall is the galactomannan (GM) which is used in diagnostic kits for the detection of specific types of aspergillosis. However, limited specificity of currently available test systems urges the need for their further improvement. Herein we report the first synthesis of branched heterosaccharides related to GM and containing α-(1→2)-/α-(1→6)-linked tetramannoside backbone chain bearing one galactofuranoside unit or its β-(1→5)-linked dimer. The preparation of conjugates of the obtained spacered oligosaccharides with BSA is also performed to produce tools for the assessment the specificity of anti-Aspergillus immune response and to select antibodies suitable for the development of novel diagnostic kits that may discriminate distinct types of aspergillosis.</p>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"549 ","pages":"109360"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selection of alkaliphilic Bacillus pectate lyases based on reactivity and pH-dependent stability in simulated environment for industrial applications.","authors":"S G Sree Agash, R Rajasekaran","doi":"10.1016/j.carres.2024.109372","DOIUrl":"10.1016/j.carres.2024.109372","url":null,"abstract":"<p><p>Pectate lyases, known for their alkaliphilic nature, are ideal for industrial applications that require specific pH conditions, particularly in industries such as textiles and pulp extraction. These enzymes, primarily from the polysaccharide lyase family 1 (PL1) of different microbial sources, play a vital role in polysaccharide degradation. Given the potent pectinolytic activity of Bacillus pectate lyases, targeting these enzymes is crucial for identifying the most effective candidates. To address challenges in enzyme selection, we examined the initial catalytic interactions of Bacillus species N16-5 (sp_N16-5), Bacillus species TS-47 (sp_TS-47), and Bacillus species subtilis strain 168 (sub_168) with pectin using molecular docking, focusing on the binding of pectin to the active-site tunnel region. We employed steered molecular dynamics simulations to analyze the disassociation period of pectin, where sp_N16-5 demonstrated higher compactness and we applied a semi-empirical quantum mechanical approach for reaction modeling. Our analysis through NPT ensemble-based dynamics analysis emphasised the structural stability and compactness required to withstand high-production conditions. We identified Bacillus species N16-5 (sp_N16-5) as the most efficient pectinolytic lyase, as it showed strong affinity, reactivity and higher interaction, also sp_N16-5 shows its enthalpy of reaction at 9 kcal/mol with a lower activation energy barrier at 27 kcal/mol which is closest to the typical range among the chosen Bacillus pectate lyase, enabling rapid pectin conversion alongside low energy input. Outcomes from the pH-dependent molecular dynamics revealed the sp_N16-5 to possess a greater structural endurability, comparatively, this study streamlines the screening process for selecting optimal Bacillus pectate lyases through in-silico investigation for industrial applications.</p>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"549 ","pages":"109372"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling cyclodextrin conjugation as multidentate excipients: An exploratory journey across industries.","authors":"Roshani Gandhi, Nishant Chopade, Prashant K Deshmukh, Rahul G Ingle, Minal Harde, Sameer Lakade, Mahesh P More, Rahul S Tade, Mahesh S Bhadane","doi":"10.1016/j.carres.2024.109357","DOIUrl":"10.1016/j.carres.2024.109357","url":null,"abstract":"<p><p>The discovery of branched molecules like dextrin by Schardinger in 1903 marked the inception of cyclodextrin (CD) utilization, catalyzing its journey from laboratory experimentation to widespread commercialization within the pharmaceutical industry. CD, a cyclic oligosaccharide containing glucopyranose units, acts as a versatile guest molecule, forming inclusion complexes (ICs) with various host molecules. Computational studies have become instrumental in elucidating the intricate interactions between β-CD and guest molecules, enabling the prediction of binding energy, forces, affinity, and complex stability. The computational approach has established robust correlations with experimental outcomes, enhancing our understanding of CD-mediated complexation phenomena. This comprehensive review delves into the CD based Inclusion complex (CDIC) formation and a myriad of components, including drug molecules, amino acids, vitamins, and volatile oils. These complexes find applications across diverse industries, ranging from pharmaceuticals to nutraceuticals, food, fragrance, and beyond. In the pharmaceutical realm, β- CDICs offer innovative solutions for enhancing drug solubility, stability, and bioavailability, thus overcoming formulation challenges associated with poorly water-soluble drugs. Furthermore, the versatility of CDs extends beyond pharmaceuticals, with applications in the encapsulation of phytoactive compounds in nutraceuticals and the enhancing flavor, aroma in food and fragrance industries. This review underscores the pivotal role of CDs conjugation in modern drug delivery systems, emphasizing the importance of interdisciplinary approaches that integrate computational modeling with experimental validation. As the pharmaceutical landscape continues to evolve, CDs-based formulations stand poised to drive innovation and address the ever-growing demand for efficacious and patient-friendly drug delivery solutions.</p>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"549 ","pages":"109357"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and synthesis of a carbohydrate-derived chemosensor for selective Ni(II) ion detection: A turn-off approach.","authors":"Aditi Arora, Sumit Kumar, Shivani Sapra, Gautam Deo, Mrityunjay K Tiwari, Brajendra K Singh, Sandeep Kumar","doi":"10.1016/j.carres.2025.109380","DOIUrl":"10.1016/j.carres.2025.109380","url":null,"abstract":"<p><p>Nickel, an essential transition metal, plays a vital role in biological systems and industries. However, exposure to nickel can cause severe health issues, such as asthma, dermatitis, pneumonitis, neurological disorders, and cancers of the nasal cavity and lungs. Due to nickel's toxicity and extensive industrial use, efficient sensors for detecting Ni²⁺ ions in environmental and biological contexts are essential. Carbohydrates, with their inherent water solubility and biocompatibility, are ideal for constructing chemosensors. Incorporating a pyridyl group enhances the selectivity and sensitivity of these sensors. We present a carbohydrate-derived colorimetric chemosensor 5-(2'-Pyridoylethene-1'-yl)-4-(2''-phenylethene-1''-yl)-2,3-O-isopropylidene-2,3-dihydrofuran-2,3-diol (7a) that exhibits a distinct colour change and significant fluorescence quenching upon binding with Ni²⁺ ions. The synthesis of receptor (7a) was validated by using ¹H, ¹³C NMR, HRMS, and single crystal X-ray analysis. Detection limit of receptor (7a) for Ni²⁺ was calculated to be 0.97 μM, which is below the standard (1.2 μM) set by the United States Environmental Protection Agency (EPA). The binding ratio of receptor (7a) to Ni²⁺ was determined to be 1:1 by using Job's plot. The binding constant of receptor (7a) and Ni²⁺ was calculated as 4.38 × 10⁴ M^-1 by using the Benesi-Hildebrand equation. This sensor demonstrates exceptional selectivity for Ni²⁺ ions over other metal cations. Receptor (7a) is stable and can be used to detect Ni²⁺ in the range of pH from 6 to 10. The sensor responded to Ni²⁺ ions selectively and a large number of coexisting ions showed almost no obvious interference with the detection. Our findings shed light on the potential of carbohydrate-derived chemosensors for nickel detection, paving the way for further exploration in this field. The binding mechanism of receptor (7a) to Ni²⁺ ions was proposed by Job's plot, UV-vis spectra and DFT (Density Functional Theory) calculations.</p>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"549 ","pages":"109380"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}