Carbohydrate Research最新文献_第2页

Electrochemical studies of Ruthenium (III) complexes derived from chitosan Schiff base ligands 壳聚糖席夫碱配体钌（III）配合物的电化学研究

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-05-26 DOI: 10.1016/j.carres.2025.109557

T. Vadivel , S. Kulathooran , S. Chandrasekaran , S. Ilayaraja , R. Benjamin

{"title":"Electrochemical studies of Ruthenium (III) complexes derived from chitosan Schiff base ligands","authors":"T. Vadivel , S. Kulathooran , S. Chandrasekaran , S. Ilayaraja , R. Benjamin","doi":"10.1016/j.carres.2025.109557","DOIUrl":"10.1016/j.carres.2025.109557","url":null,"abstract":"<div><div>The biocompatible nature of the chitosan biopolymer enables it to facilitate a wide range of organic transformations including chemical modification with some carbonyl compounds. Transition metal complexes namely [Ru(CS)4hy3mbd)(H<sub>2</sub>O)<sub>2</sub>].Cl<sub>2,</sub> [Ru(CS)2hybd)(H<sub>2</sub>O)<sub>2</sub>].Cl<sub>2</sub>, and [Ru(CS)2hy3mbd)(H<sub>2</sub>O)<sub>2</sub>].Cl<sub>2</sub> were synthesized by a complexation of bidentate Schiff base ligands which contains nitrogen and oxygen donor atoms. The nitrogen atom of azomethine linkage and the oxygen atom of aromatic aldehydes are the active sites for complexation reactions to form stable coordination complexes with ruthenium metal ions. The electronic transitions of ruthenium (III) complexes were studied by UV–Vis spectroscopy. The functional groups analysis were performed with FT-IR spectrum. The electronic structure and hyperfine splitting pattern of the metal complexes were discussed with ESR spectral data. The proton environment of the complex molecules were studied by 1H NMR spectroscopy. The stable complexes have shown a good crystalline nature which are characterized by powder XRD. Thermal stability of complexes was studied by Thermo-gravimetry analysis. The electrochemical property of ruthenium metal complexes were discussed with cyclic voltammogram and this study helps to distinguish reversible, quasi reversible redox properties of the metal complexes derived from biopolymer based Schiff base ligands.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"554 ","pages":"Article 109557"},"PeriodicalIF":2.4,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterization of halotolerant multifunctional GH6 endoglucanases ZFEG1605 and ZFEG1663 from Mt. Everest soil metagenome 珠穆朗玛峰土壤宏基因组GH6耐盐多功能内切葡聚糖酶ZFEG1605和ZFEG1663的鉴定与表征

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-05-24 DOI: 10.1016/j.carres.2025.109549

Justice K. Rono , Qingyun Zhang , Yong He , Leqi Zhao , Shaochen Wang , Yunbin Lyu , Chunyang Li , Anastasiah N. Ngigi , Zhi Min Yang , Zhiyang Feng

{"title":"Identification and characterization of halotolerant multifunctional GH6 endoglucanases ZFEG1605 and ZFEG1663 from Mt. Everest soil metagenome","authors":"Justice K. Rono , Qingyun Zhang , Yong He , Leqi Zhao , Shaochen Wang , Yunbin Lyu , Chunyang Li , Anastasiah N. Ngigi , Zhi Min Yang , Zhiyang Feng","doi":"10.1016/j.carres.2025.109549","DOIUrl":"10.1016/j.carres.2025.109549","url":null,"abstract":"<div><div>Environmental microorganisms express enzymes with unique hydrolytic activity, stability, and kinetic parameters, which are of great interest for biotechnological applications. In this study, two novel endoglucanases, ZFEG1605 and ZFEG1663, were cloned from Mt. Everest soil metagenomic library, heterologously expressed in <em>E. coli</em> BL21(DE3), and characterized. Both enzymes exhibited high activity on konjac glucommanan (KG) and sodium carboxymethylcellulose (CMC), while ZFEG1605 also exhibited activity towards guar gum (GG). The optimal pH for both enzymes was slightly shifted toward acidic range (pH 5/6). The optimal reaction temperatures for ZFEG1605 and ZFEG1663 were 50 and 40 °C, respectively. ZFEG1605 was more thermostable than ZFEG1663 as it remained stable up to 50 °C, compared to 40 °C for ZFEG1663. Both enzymes showed broad pH stability, although they retained more mannanase activity than CMCase activity within the same pH range. The endoglucanases exhibited remarkable salt tolerance, retaining over 70 % of their enzymatic activity in the presence of 2.5 M NaCl. The purified enzymes hydrolyzed alkali-pretreated rice straw to release reducing sugars, demonstrating their potential usage for biomass saccharification.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"554 ","pages":"Article 109549"},"PeriodicalIF":2.4,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural polysaccharide-based injectable hydrogels with tunable mechanical and electrical properties enabled by phytic acid 天然多糖为基础的注射水凝胶，具有可调的机械和电气性能，由植酸实现

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-05-23 DOI: 10.1016/j.carres.2025.109555

Zhijie Geng , Cuiping Guo , Daohuan Lu , Shan Yu , Jun Huang

{"title":"Natural polysaccharide-based injectable hydrogels with tunable mechanical and electrical properties enabled by phytic acid","authors":"Zhijie Geng , Cuiping Guo , Daohuan Lu , Shan Yu , Jun Huang","doi":"10.1016/j.carres.2025.109555","DOIUrl":"10.1016/j.carres.2025.109555","url":null,"abstract":"<div><div>A fully natural polysaccharide-based hydrogel with tunable mechanical and electrical properties was developed by introducing phytic acid (PA) into a dynamic Schiff-base network formed between chitosan (CS) and oxidized sodium alginate (OSA), using a simple and scalable post-treatment strategy. Unlike conventional methods that require complex chemical modifications or synthetic conductive additives, this approach leverages the inherent multifunctionality of PA to simultaneously achieve structural reinforcement and ionic conductivity enhancement through a single, mild, and chemical-free immersion process, representing a significant advancement in hydrogel design. The incorporation of PA significantly enhanced the mechanical strength of the hydrogel (12.6 kPa up to 881.8 kPa), reduced swelling (from 9185.6 ± 127.0 % to 135.2 ± 26.0 %), and improved degradation resistance (mass loss reduced from 93.2 ± 3.1 % to 7.5 ± 0.9 %). Moreover, the presence of dissociated phosphate groups from PA introduced mobile ionic species, leading to increased ionic conductivity and stable strain-responsive electrical signals. The hydrogel also exhibited excellent cytocompatibility, low hemolysis ratios (<5 %), and strong antioxidant activity, particularly at optimized PA concentrations. These enhancements were attributed to PA-mediated multi-point hydrogen bonding and electrostatic interactions, which reinforced the network structure and promoted ionic mobility. This work not only demonstrates an effective strategy for modulating hydrogel properties in a green and scalable manner, but also expands the application potential of natural polysaccharide hydrogels by offering a previously underexplored yet highly efficient route for achieving multifunctionality.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"554 ","pages":"Article 109555"},"PeriodicalIF":2.4,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immobilization of lipase on biodegradable starch-based membrane and its application 可生物降解淀粉基膜固定化脂肪酶及其应用

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-05-23 DOI: 10.1016/j.carres.2025.109553

Aparna Dhavade , Sarika Hinge , Sushma Sabharwal

{"title":"Immobilization of lipase on biodegradable starch-based membrane and its application","authors":"Aparna Dhavade , Sarika Hinge , Sushma Sabharwal","doi":"10.1016/j.carres.2025.109553","DOIUrl":"10.1016/j.carres.2025.109553","url":null,"abstract":"<div><div>Global demand for the lipase enzyme is trending and anticipated to rise USD 1258.89 million by 2031. Immobilization of lipase enzymes on a solid support is useful in various applications, viz., medical, food, wine, waste treatment, biodiesel, cosmetics, perfumes, biodegradable polymers, etc. Therefore, in the present study, starch was used to prepare membranes and investigate the efficacy of starch as an inert solid support for enzyme immobilization. <em>Aspergillus niger</em> lipase cross-linked with starch support and enzyme immobilization was optimized for various parameters, viz., different cross linkers, percent of cross-linkers, concentration of enzyme, etc. Reusability of the lipase-immobilized film was obtained 11 times, which is sensitive to detect insecticides. Surface morphology of starch and lipase-immobilized starch films were examined by scanning electron microscopy, and functional groups involved in immobilization was determined by FTIR. A comparative study of the biochemical parameters, viz., optimum pH, temperature, effect of metal ions, and organic solvents on the native and immobilized lipase, was carried out. Stability of immobilized and native lipase activity in different organic media was evaluated for different time intervals. Thermal stability was checked at optimum temperature (50 <sup>°</sup>C) for various time intervals. Biodegradability of membranes was tested with soil and different enzymes. Morphological changes were observed by FE-SEM. Immobilized lipase is a good source to sense the presence of pesticides in soil; therefore, preliminary studies have been carried out.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"554 ","pages":"Article 109553"},"PeriodicalIF":2.4,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

More than a protective group: 4-methoxyphenyl as a new Janus aglycone 多一个保护基团：4-甲氧基苯基作为新雅努斯苷元

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-05-22 DOI: 10.1016/j.carres.2025.109544

Bogdan L. Novosad, Alexander I. Zinin, Alexander O. Chizhov, Leonid O. Kononov

引用次数: 0

Biosynthesis of novel anticoagulant substances, α-salicin and α-isosalicin, using sucrose phosphorylase 利用蔗糖磷酸化酶生物合成新型抗凝血物质α-水杨苷和α-异水杨苷

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-05-21 DOI: 10.1016/j.carres.2025.109547

Yuxin Cheng , Jingjing Sun , Wei Wang , Chengcheng Jiang , Jianhua Hao

{"title":"Biosynthesis of novel anticoagulant substances, α-salicin and α-isosalicin, using sucrose phosphorylase","authors":"Yuxin Cheng , Jingjing Sun , Wei Wang , Chengcheng Jiang , Jianhua Hao","doi":"10.1016/j.carres.2025.109547","DOIUrl":"10.1016/j.carres.2025.109547","url":null,"abstract":"<div><div>This study reports the enzymatic synthesis of novel anticoagulant glycosides, α-salicin and α-isosalicin, through the transglycosylation of <em>o</em>-hydroxybenzyl alcohol (salicyl alcohol) catalyzed by marine-derived sucrose phosphorylase Suc75290. Under optimized conditions (240 g/L sucrose, 12 g/L <em>o</em>-hydroxybenzyl alcohol, 150 U/mL enzyme, pH 7.5, 45 °C, 20 h), a conversion rate of 79.17 ± 0.64 % was achieved, yielding 8.57 ± 0.33 g/L α-salicin and 0.52 ± 0.19 g/L α-isosalicin. Structural characterization by NMR confirmed the α-configuration of both compounds. In vitro anticoagulant assays demonstrated that these glycosides significantly prolonged activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT), while reducing fibrinogen (FIB) levels, indicating dual inhibition of intrinsic and extrinsic coagulation pathways. Molecular docking revealed key interactions between salicyl alcohol and Suc75290's active site. This work establishes an efficient enzymatic route to α-anomeric salicin analogs with therapeutic potential, circumventing the limitations of chemical synthesis. These two novel anticoagulant glycoside compounds enzymatically synthesized provide new research ideas for the development of anticoagulant drugs.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"554 ","pages":"Article 109547"},"PeriodicalIF":2.4,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced enzymatic hydrolysis of corn stalk and corn cob with deep eutectic solvent pretreatment 深度共熔溶剂预处理对玉米秸秆和玉米芯酶解的促进作用

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-05-21 DOI: 10.1016/j.carres.2025.109548

Yupeng Liu (刘玉朋), Lu'an Xu (徐庐安), Shenlong Gao (高慎龙), Yutong Qu (屈禹彤), Jinchen Zheng (郑金晨), Weijun Li (李维军), Mengjiao Zhao (赵孟姣)

{"title":"Enhanced enzymatic hydrolysis of corn stalk and corn cob with deep eutectic solvent pretreatment","authors":"Yupeng Liu (刘玉朋), Lu'an Xu (徐庐安), Shenlong Gao (高慎龙), Yutong Qu (屈禹彤), Jinchen Zheng (郑金晨), Weijun Li (李维军), Mengjiao Zhao (赵孟姣)","doi":"10.1016/j.carres.2025.109548","DOIUrl":"10.1016/j.carres.2025.109548","url":null,"abstract":"<div><div>Deep eutectic solvent including Choline chloride (ChCl)/Ethylene glycol (EG) and betaine HCL (BH)/EG and the temperature range of 80–100 °C for 1–7h was deemed suitable for enhancing the enzymatic saccharification efficiency of corn stalk and corn cob. The effectiveness of the pretreatment was established by the extent of lignin removal and the sugar yield of enzymatic hydrolysis. The results showed that both ChCl/EG and BH/EG pretreatments could boost the sugar yields from the two biomass, the sugar yields were reached 60.06 %, 58.33 %, 76.8 % and 67.03 %, respectively, under the best conditions. The saccharification yield from enzymatic hydrolysis of corn stalk and corn cob, subjected to ChCl/EG and BH/EG pretreatments, escalated by 42.36 %、40.63 % and 59.1 %、49.33 %, respectively, in comparison to the control samples. Post-pretreatment, X-ray diffraction (XRD) analyses revealed no significant alterations in the crystalline lattice structure of the corn stalk and corn cob samples. In contrast, scanning electron microscopy (SEM) visualisations depicted a pronounced modification in the micro- and nano-architecture of the lignocellulosic biomass.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"555 ","pages":"Article 109548"},"PeriodicalIF":2.4,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144204629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Homogeneous sulfation of polyguluronic acids (poly-G-s) with Py·SO3 in DMSO and their characterization with NMR-spectroscopy 聚谷醛酸（聚g -s）与Py·SO3在DMSO中的均匀磺化及其核磁共振表征

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-05-21 DOI: 10.1016/j.carres.2025.109543

Karl M. Ingerma , Indrek Reile , Angelica Närep , Rando Tuvikene

{"title":"Homogeneous sulfation of polyguluronic acids (poly-G-s) with Py·SO3 in DMSO and their characterization with NMR-spectroscopy","authors":"Karl M. Ingerma , Indrek Reile , Angelica Närep , Rando Tuvikene","doi":"10.1016/j.carres.2025.109543","DOIUrl":"10.1016/j.carres.2025.109543","url":null,"abstract":"<div><div>Sulfated polyguluronates (poly-G) have shown promising bioactivities, including immunomodulatory, anticoagulative, antiviral and anti-inflammatory activity. However they have seen only a limited amount of structural characterization, making it difficult to determine structure-activity relationships and thus complicating their application into practice. Therefore poly-G-s were sulfated with Py·SO<sub>3</sub> in DMSO and the resulting derivatives were systematically characterized with NMR-spectroscopy to assign the signals of sulfated residues and to identify how different sulfation patterns manifest in NMR spectra. With lower concentration of the sulfating reagent, a slight preference for G-3 was found, while with higher concentration, G-2 preference increased alongside G-2,3-disulfation. Protecting groups (PG) were additionally used to direct selectivity and thus aid structural characterization. A degree of G-2 sulfation preference was achieved with TBDMS-ether as PG, while with Bz-ester PG, G-3 preference was achieved. Degradation by β-elimination of poly-G was found to be minimal under the developed experimental conditions.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"554 ","pages":"Article 109543"},"PeriodicalIF":2.4,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering PEG-alginate microcarriers for precision drug Delivery: Optimized design and controlled release 工程聚乙二醇海藻酸盐微载体的精确给药：优化设计和控释

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-05-20 DOI: 10.1016/j.carres.2025.109546

Hossein Madineh , Nadereh Golshan Ebrahimi , Payam Zarrintaj

{"title":"Engineering PEG-alginate microcarriers for precision drug Delivery: Optimized design and controlled release","authors":"Hossein Madineh , Nadereh Golshan Ebrahimi , Payam Zarrintaj","doi":"10.1016/j.carres.2025.109546","DOIUrl":"10.1016/j.carres.2025.109546","url":null,"abstract":"<div><div>In recent years, polymeric microcarriers have emerged as a targeted approach for controlled drug release. With advantages such as increased drug delivery efficiency, controlled release mechanisms, exceptional stability, and targeted release capabilities, microcarriers have become a focal point of research in drug delivery strategies. In this study, polyethylene glycol (PEG) was first functionalized with sialic acid (SA), and the resulting structure was evaluated using FTIR analysis. The dropwise extrusion method was utilized to form the microcarriers. We employed the central composite design (CCD) method to optimize the fabrication of polymeric microcarriers. Finally optimized microcarriers were prepared with an average particle size of 880 ± 2 <span><math><mrow><mi>μ</mi><mi>m</mi><mtext>.</mtext></mrow></math></span> In the optimal microcarrier, the amounts of loaded drug and its efficiency were 90 % and 91 %, respectively. The results indicated the release of curcumin in the prepared system compared to free curcumin was controlled and long-lasting. The utilization of polymeric microcarriers as a targeted approach for controlled drug release holds great promise in revolutionizing the field of drug delivery. This research not only enhances our understanding of the potential applications of microcarriers but also opens up new horizons for advancing controlled drug release strategies, ultimately leading to more effective and personalized treatments for various diseases.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"554 ","pages":"Article 109546"},"PeriodicalIF":2.4,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144147533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitin recognition by a periplasmic chitooligosaccharide-binding protein from Vibrio cholerae 霍乱弧菌壳寡糖结合蛋白对几丁质的识别

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-05-20 DOI: 10.1016/j.carres.2025.109552

Takayuki Ohnuma , Teruki Yoshimoto , Wipa Suginta , Tamo Fukamizo

{"title":"Chitin recognition by a periplasmic chitooligosaccharide-binding protein from Vibrio cholerae","authors":"Takayuki Ohnuma , Teruki Yoshimoto , Wipa Suginta , Tamo Fukamizo","doi":"10.1016/j.carres.2025.109552","DOIUrl":"10.1016/j.carres.2025.109552","url":null,"abstract":"<div><div>The mechanism of chitin recognition by a periplasmic chitooligosaccharide-binding protein from Vibrio cholerae (VcCBP) was studied by thermal shift assays and isothermal titration calorimetry using di-N-acetylchitobiose, (GlcNAc)2; mono-N-acetylchitobioses, GlcN-GlcNAc and GlcNAc-GlcN; and fully de-N-acetylated chitobiose, (GlcN)2; as the ligands. As judged from the thermal shifts (ΔTm) of VcCBP upon the addition of individual chitobioses, the binding abilities toward VcCBP appeared to decrease in the order of (GlcNAc)2 > GlcN-GlcNAc > GlcNAc-GlcN ≫ (GlcN)2. Although the de-N-acetylation effect of the reducing end GlcNAc was more significant than that of the non-reducing end, both N-acetyl groups were found to cooperatively contribute to the interaction between VcCBP and (GlcNAc)2. The binding affinity of GlcN-GlcNAc to VcCBP was lower than that of (GlcNAc)2 by only 0.5 kcal·mol-1 of ΔG°; however, the entropy gain (-TΔS°) was enhanced in the former compared with the latter. GlcN-GlcNAc are likely to bind loosely to VcCBP but unlikely to undergo translocation by the VcCBP-mediated transporter system.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"554 ","pages":"Article 109552"},"PeriodicalIF":2.4,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144147534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0