Enhanced enzymatic hydrolysis of corn stalk and corn cob with deep eutectic solvent pretreatment

IF 2.4 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research Pub Date : 2025-05-21 DOI:10.1016/j.carres.2025.109548

Yupeng Liu (刘玉朋), Lu'an Xu (徐庐安), Shenlong Gao (高慎龙), Yutong Qu (屈禹彤), Jinchen Zheng (郑金晨), Weijun Li (李维军), Mengjiao Zhao (赵孟姣)

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引用次数: 0

Abstract

Deep eutectic solvent including Choline chloride (ChCl)/Ethylene glycol (EG) and betaine HCL (BH)/EG and the temperature range of 80–100 °C for 1–7h was deemed suitable for enhancing the enzymatic saccharification efficiency of corn stalk and corn cob. The effectiveness of the pretreatment was established by the extent of lignin removal and the sugar yield of enzymatic hydrolysis. The results showed that both ChCl/EG and BH/EG pretreatments could boost the sugar yields from the two biomass, the sugar yields were reached 60.06 %, 58.33 %, 76.8 % and 67.03 %, respectively, under the best conditions. The saccharification yield from enzymatic hydrolysis of corn stalk and corn cob, subjected to ChCl/EG and BH/EG pretreatments, escalated by 42.36 %、40.63 % and 59.1 %、49.33 %, respectively, in comparison to the control samples. Post-pretreatment, X-ray diffraction (XRD) analyses revealed no significant alterations in the crystalline lattice structure of the corn stalk and corn cob samples. In contrast, scanning electron microscopy (SEM) visualisations depicted a pronounced modification in the micro- and nano-architecture of the lignocellulosic biomass.

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深度共熔溶剂预处理对玉米秸秆和玉米芯酶解的促进作用

采用氯化胆碱(ChCl)/乙二醇（EG）和甜菜碱(HCL)/EG为深度共晶溶剂，温度范围为80 ~ 100℃，反应时间为1 ~ 7h，可提高玉米秸秆和玉米芯的酶解糖化效率。通过木质素去除率和酶解产糖率确定了预处理的有效性。结果表明，ChCl/EG和BH/EG预处理均可提高两种生物质的糖收率，最佳预处理条件下糖收率分别达到60.06%、58.33%、76.8%和67.03%。经ChCl/EG和BH/EG预处理的玉米秸秆和玉米芯的酶解糖化率分别比对照样品提高了42.36%、40.63%和59.1%、49.33%。预处理后的x射线衍射（XRD）分析显示，玉米秸秆和玉米芯样品的晶格结构没有明显变化。相比之下，扫描电子显微镜（SEM）可视化描述了木质纤维素生物质的微观和纳米结构的明显改变。

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来源期刊

Carbohydrate Research 化学-生化与分子生物学

CiteScore

5.00

自引率

3.20%

发文量

183

审稿时长

3.6 weeks

期刊介绍： Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".