霍乱弧菌壳寡糖结合蛋白对几丁质的识别

IF 2.4 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research Pub Date : 2025-05-20 DOI:10.1016/j.carres.2025.109552

Takayuki Ohnuma , Teruki Yoshimoto , Wipa Suginta , Tamo Fukamizo

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引用次数: 0

摘要

采用热移法和等温滴定量热法研究了霍乱弧菌壳寡糖结合蛋白（VcCBP）对几丁质的识别机制；单n -乙酰基壳聚糖、GlcN-GlcNAc和GlcNAc-GlcN；完全去n -乙酰化壳聚糖（GlcN）2；作为配体。从添加单个壳聚糖后VcCBP的热位移（ΔTm）判断，对VcCBP的结合能力表现为（GlcNAc）2 >；GlcN-GlcNAc祝辞GlcNAc-GlcN > （GlcN）虽然还原端GlcNAc的去n-乙酰化作用比非还原端更显著，但两个n-乙酰基都协同促进了VcCBP与（GlcNAc）2的相互作用。GlcN-GlcNAc与VcCBP的结合亲和力仅比（GlcNAc）2低0.5 kcal·mol-1 （ΔG°）；但前者的熵增益（-TΔS°）比后者有所提高。GlcN-GlcNAc可能松散地与VcCBP结合，但不太可能通过VcCBP介导的转运体系统进行易位。

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Chitin recognition by a periplasmic chitooligosaccharide-binding protein from Vibrio cholerae

The mechanism of chitin recognition by a periplasmic chitooligosaccharide-binding protein from Vibrio cholerae (VcCBP) was studied by thermal shift assays and isothermal titration calorimetry using di-N-acetylchitobiose, (GlcNAc)2; mono-N-acetylchitobioses, GlcN-GlcNAc and GlcNAc-GlcN; and fully de-N-acetylated chitobiose, (GlcN)2; as the ligands. As judged from the thermal shifts (ΔTm) of VcCBP upon the addition of individual chitobioses, the binding abilities toward VcCBP appeared to decrease in the order of (GlcNAc)2 > GlcN-GlcNAc > GlcNAc-GlcN ≫ (GlcN)2. Although the de-N-acetylation effect of the reducing end GlcNAc was more significant than that of the non-reducing end, both N-acetyl groups were found to cooperatively contribute to the interaction between VcCBP and (GlcNAc)2. The binding affinity of GlcN-GlcNAc to VcCBP was lower than that of (GlcNAc)2 by only 0.5 kcal·mol-1 of ΔG°; however, the entropy gain (-TΔS°) was enhanced in the former compared with the latter. GlcN-GlcNAc are likely to bind loosely to VcCBP but unlikely to undergo translocation by the VcCBP-mediated transporter system.

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来源期刊

Carbohydrate Research 化学-生化与分子生物学

CiteScore

5.00

自引率

3.20%

发文量

183

审稿时长

3.6 weeks

期刊介绍： Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".