Carbohydrate Research最新文献_第10页

Structures of W77F/W212F and W77F/W212F Toxascaris leonine galectin complex with glucose W77F/W212F和W77F/W212F狮子座弓形虫凝集素与葡萄糖复合物的结构

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2025-12-01 Epub Date: 2025-09-02 DOI: 10.1016/j.carres.2025.109657

Min Seon Ha , Chang Woo Han , Mi Suk Jeong , Se Bok Jang

引用次数: 0

Identification, purification and functional characterization of a thermostable marine chitinase for potential fungal control via chitin degradation mechanism 一种耐热性海洋几丁质酶的鉴定、纯化及功能表征

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2025-12-01 Epub Date: 2025-09-03 DOI: 10.1016/j.carres.2025.109654

Atheena P.V. , Keyur Raval , Ritu Raval

{"title":"Identification, purification and functional characterization of a thermostable marine chitinase for potential fungal control via chitin degradation mechanism","authors":"Atheena P.V. , Keyur Raval , Ritu Raval","doi":"10.1016/j.carres.2025.109654","DOIUrl":"10.1016/j.carres.2025.109654","url":null,"abstract":"<div><div>The growing prevalence of treatment-resistant <em>Candida</em> species highlights an urgent need for innovative antifungal therapies. The current range of antifungals, limited to polyenes, azoles, and echinocandins, are becoming insufficient due to the rise of resistance, including cross-resistance among fungal strains. Marine environment is an underexplored reservoir of unique enzymes which can be extremophilic. This study presents the cloning and expression of a chitinase gene from the bacterium <em>Bacillus thuringiensis</em> (<em>Bt</em>Chi), expressed in an <em>E. coli</em> system, yielding a protein with a molecular weight of approximately 71 kDa. Disc diffusion and MIC experiments indicated that 5 μg/mL chitinase efficiently suppressed the growth of <em>Candida albicans.</em> Initial characterization identified the optimal activity at 40 °C and pH 7.0. The enzyme retained over 75 % activity across a pH range of 4–8 and a temperature range of 30–70 °C after 120 min. Activity was further enhanced by 24 % with 100 mM Na<sup>+</sup>. Kinetic parameters with colloidal chitin revealed K<sub>m</sub> and V<sub>max</sub> values to be 0.05 mg/mL and 1.37 U/mL respectively. This study holds the potential of developing a potent natural anti-fungal against the present day chemical counterparts.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"558 ","pages":"Article 109654"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144997473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First total synthesis of cyanopterin, a pterin glycoside isolated from a cyanobacterium 首次合成了从一种蓝藻菌中分离出来的蝶呤苷

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2025-12-01 Epub Date: 2025-10-21 DOI: 10.1016/j.carres.2025.109710

Tadashi Hanaya, Yuta Maeda, Kazumasa Ejiri

引用次数: 0

Editorial – VSI: Chitosan and its derivatives: biomedicine and beyond 编辑- VSI：壳聚糖及其衍生物：生物医学及其应用。

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2025-12-01 Epub Date: 2025-09-11 DOI: 10.1016/j.carres.2025.109669

引用次数: 0

Silicon-polysaccharide fluorescent nanocomposite for everolimus delivery and theranostic application in clear cell renal cell carcinoma 硅-多糖荧光纳米复合物在透明细胞肾细胞癌中依维莫司的递送和治疗应用

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2025-12-01 Epub Date: 2025-09-25 DOI: 10.1016/j.carres.2025.109688

Zhihong Lv, Yuping Xu, Huiying Liu, Hui Li, Hongyan Zhai

{"title":"Silicon-polysaccharide fluorescent nanocomposite for everolimus delivery and theranostic application in clear cell renal cell carcinoma","authors":"Zhihong Lv, Yuping Xu, Huiying Liu, Hui Li, Hongyan Zhai","doi":"10.1016/j.carres.2025.109688","DOIUrl":"10.1016/j.carres.2025.109688","url":null,"abstract":"<div><div>Everolimus showed therapeutic promise in treating clear cell renal cell carcinoma (ccRCC), yet its efficacy was limited by poor bioavailability and weak tumor targeting. In this study, we developed a multifunctional Co(II)-based MOF (CP1) using H<sub>4</sub>L and bpp ligands, forming a 3D (4,8)-connected framework. To enhance stability and functionality, CP1 was coated with sodium alginate (SA) and APTMS, and further modified with a fluorescent benzopyranone derivative (compound 1) via Ullmann-type coupling, yielding 1-SA-APTMS@CP1. The final nanocarrier, 1-SA-APTMS@CP1@EVE, exhibited a high surface area (98.3 m<sup>2</sup>/g), mesoporosity (2–6 nm), and strong structural integrity. It enabled dual-channel fluorescence detection of GSH and NAD(P)H, with detection limits of 233 pM and 65 nM, respectively. In vitro, the system effectively inhibited ccRCC cell proliferation and downregulated TNFRSF18, a key immunomodulatory gene. This study provides a redox-responsive, fluorescence-trackable delivery platform for everolimus, offering potential for targeted and immune-synergistic therapy in ccRCC.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"558 ","pages":"Article 109688"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resveratrol-loaded nanoparticles based on the self-assembly behavior of Sparassis latifolia polysaccharides: Preparation, characterization, release behavior under in vitro digestion and synergistic hypoglycemic effects 白藜芦醇负载纳米粒子的自组装行为：制备、表征、体外消化释放行为和协同降糖作用。

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2025-12-01 Epub Date: 2025-10-20 DOI: 10.1016/j.carres.2025.109707

Pengya Du , Shuyu Bi , Fangming Cui , Lixia He , Jiangyan Hou , Jinling Cao , Yanfen Cheng , Feier Cheng , Wenfei Zhao , Cuiping Feng , Shaojun Yun

{"title":"Resveratrol-loaded nanoparticles based on the self-assembly behavior of Sparassis latifolia polysaccharides: Preparation, characterization, release behavior under in vitro digestion and synergistic hypoglycemic effects","authors":"Pengya Du , Shuyu Bi , Fangming Cui , Lixia He , Jiangyan Hou , Jinling Cao , Yanfen Cheng , Feier Cheng , Wenfei Zhao , Cuiping Feng , Shaojun Yun","doi":"10.1016/j.carres.2025.109707","DOIUrl":"10.1016/j.carres.2025.109707","url":null,"abstract":"<div><div>Resveratrol (Res) is a naturally occurring polyphenolic compound exhibiting a wide range of biological activities. However, its application is limited due to the instability, poor water solubility, and low bioavailability. Therefore, this study aimed to prepare <em>Sparassis latifolia</em> polysaccharides (SLPs) nanoparticles loaded with Res (SLPs-Res NPs) through the self-assembly behavior of SLPs. Our findings revealed that SLPs underwent a conformational transition from triple helix to single strand when exposed to varying concentrations of dimethyl sulfoxide (DMSO). By virtue of this self-assembly characteristic, the spherical or subspherical nanoparticles loaded with Res were successfully fabricated. The optimal preparation conditions included an 80 % volume ratio of DMSO, a mass ratio of Res to SLPs at 1:32, and magnetic stirring for 2 h. Under these conditions, the average particle size of SLPs-Res NPs was 230.4 ± 7.8 nm, and the encapsulation efficiency of Res reached (66.69 ± 0.10)%. Hydrogen bonding and hydrophobic forces were involved in the formation of nanoparticles. SLP-Res NPs demonstrated enhanced photothermal stability and desirable sustained-release properties. It also exerted significant inhibitory effects on α-glucosidase activity <em>in vitro</em> and glucose-lowering effects in mice during glucose tolerance tests. This study presents an approach for delivering Res using self-assembled SLPs, demonstrating their potential as effective functional carriers for the encapsulation of hydrophobic bioactive molecules.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"558 ","pages":"Article 109707"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145408033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant-based biodegradable and biocompatible polymers for tissue engineering applications 用于组织工程应用的植物基可生物降解和生物相容性聚合物。

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2025-12-01 Epub Date: 2025-10-06 DOI: 10.1016/j.carres.2025.109699

Aditya Teja Guduru, Subramanian Sankaranarayanan, Dhiraj Bhatia

{"title":"Plant-based biodegradable and biocompatible polymers for tissue engineering applications","authors":"Aditya Teja Guduru, Subramanian Sankaranarayanan, Dhiraj Bhatia","doi":"10.1016/j.carres.2025.109699","DOIUrl":"10.1016/j.carres.2025.109699","url":null,"abstract":"<div><div>Tissue engineering has emerged as a potential area in regenerative medicine, leveraging biodegradable and biocompatible polymers to fabricate scaffolds for cell growth and tissue regeneration. Among biomaterials, plant-based polymers have garnered significant attention due to their sustainability, biocompatibility, and suitable mechanical properties. This review emphasises the innovative modifications in natural polymers such as fungal chitosan, nanocellulose composites, and hybrid plant-synthetic scaffolds-that address longstanding challenges in mechanical stability, degradation control, and bioactivity. By systematically comparing polysaccharides (e.g., cellulose, alginate) and proteins (soy, zein) across bone, cartilage, and wound healing applications, we identify structure-function relationships that enable tailored scaffold design. Finally, this review critically evaluates recent advances in plant-based polymers for tissue engineering, highlighting innovative modifications and unresolved challenges providing actionable strategies to advance plant derived biomaterials towards clinical transition.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"558 ","pages":"Article 109699"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145291210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IgG galactosylation changes in cancer IgG半乳糖基化在癌症中的变化。

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2025-12-01 Epub Date: 2025-09-22 DOI: 10.1016/j.carres.2025.109681

Yumeng Liu , Xiequn Xu , Zejian Zhang

{"title":"IgG galactosylation changes in cancer","authors":"Yumeng Liu , Xiequn Xu , Zejian Zhang","doi":"10.1016/j.carres.2025.109681","DOIUrl":"10.1016/j.carres.2025.109681","url":null,"abstract":"<div><div>The incidence and mortality rates of cancer continue to rise globally. To facilitate earlier diagnosis and treatment, and thereby improve the survival outcomes for cancer patients, numerous detection methods have been employed into clinical practice. Glycosylation, a common biological regulatory process, plays a key role in both physiological and pathological processes. Glycans are oligosaccharides composed of a variety of monosaccharides, and galactose is an important terminal structure of glycans. Notably, increased agalactosylated IgG is commonly associated with the occurrence and progression of cancers; however, the clinical utility of IgG galactosylation in cancer remains a contentious topic. This review summarizes current evidence on alternations in serum and plasma IgG galactosylation in cancer patients, discusses potential mechanisms underlying these changes, and highlights future use in cancer detection, diagnosis, and prognosis. Overall, IgG galactosylation holds significant promise not only for the diagnosis of malignant tumors but also for distinguishing between benign and malignant tumors, cancer staging and monitoring progression. IgG galactosylation could serve as a complementary parameter to existing cancer markers, thereby contributing to more precise and timely diagnosis and treatment of cancers.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"558 ","pages":"Article 109681"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145198396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the physical and chemical features of composites produced from textile waste and cellulose plants 纺织废料与纤维素植物合成复合材料的理化特性比较。

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2025-12-01 Epub Date: 2025-10-21 DOI: 10.1016/j.carres.2025.109706

Norbert Lipka , Paulina Żarnowiec , Karolina Soja , Wiesław Kaca

{"title":"Comparison of the physical and chemical features of composites produced from textile waste and cellulose plants","authors":"Norbert Lipka , Paulina Żarnowiec , Karolina Soja , Wiesław Kaca","doi":"10.1016/j.carres.2025.109706","DOIUrl":"10.1016/j.carres.2025.109706","url":null,"abstract":"<div><div>One of the most pressing unresolved environmental challenges is the massive accumulation of unrecycled textile waste. In this study, we demonstrate that reinforcing recycled textile materials with plant-based cellulose fibers can yield functional composite materials. The following natural fibers were investigated: sisal, cotton, linen, coconut, wood, abaca, jute, and hemp. Both the textiles and plant fibers were ground into fragments ranging from 2 to 15 mm in length. Plant–textile composites were produced using an extruder at temperatures up to 195 °C, allowing for the plasticization of the polymer components in the textile waste. Plant fibers were incorporated into the recycled textile matrix at concentrations ranging from 3 % to 6 % by weight. A total of 19 different composite samples were fabricated and tested for their mechanical properties, including tensile strength, flexural strength, and compressive resistance.</div><div>FTIR spectra of all cellulose fibers exhibited characteristic cellulose absorption bands, with notable differences in intensity and distribution. The O–H stretching band (3600–3200 cm<sup>−1</sup>), indicative of hydrogen bonding within cellulose, varied significantly across the different fiber types. SEM micrographs revealed that the surface of the sisal-reinforced composite was relatively smooth and uniform, while the fiberglass composite exhibited a rough and crinkled surface morphology.</div><div>In conclusion, composites made from recycled textiles and plant-based fibers represent a promising class of eco-friendly materials with potential as sustainable alternatives to conventional composites.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"558 ","pages":"Article 109706"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145354007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular dynamics insights into glycosaminoglycan effects on the extracellular domains of syndecan 2 and 4 dimers 糖胺聚糖对syndecan 2和4二聚体胞外结构域影响的分子动力学研究。

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2025-12-01 Epub Date: 2025-09-30 DOI: 10.1016/j.carres.2025.109690

Sergey A. Samsonov , Sylvie Ricard-Blum

{"title":"Molecular dynamics insights into glycosaminoglycan effects on the extracellular domains of syndecan 2 and 4 dimers","authors":"Sergey A. Samsonov , Sylvie Ricard-Blum","doi":"10.1016/j.carres.2025.109690","DOIUrl":"10.1016/j.carres.2025.109690","url":null,"abstract":"<div><div>The four mammalian syndecans (1–4) are transmembrane proteoglycans, which all bear three heparan sulfate chains covalently attached to their core protein. They contribute to cell signaling, and play a role in numerous diseases. The syndecans dimerize via their transmembrane domains, and their cytoplasmic domains form a compact intertwined dimer. This computational study aims at building models of the dimers of the extracellular domain of syndecans 2 and 4, and to determine if and how the glycosaminoglycan (GAG) chains attached to their core proteins affect the dimer structural and dynamic properties. The GAGosylation was mimicked by adding three heparin hexadecasaccharide chains to each extracellular domain. The initial conformations of the dimers, and specifically the distances between the C-termini of the extracellular domains, were defined using our SAXS experimental data and literature data. The four generated models (<em>i.e.</em>, dimers of the extracellular domains of syndecans 2 and 4 with or without GAG chains) were then subjected to multiple independent microsecond-scale molecular dynamics simulations in implicit solvent, and their trajectories were analyzed. The structural and dynamic descriptors calculated from the ensembles of the four models followed unimodal distributions. No well-defined conformational states were observed. GAGosylation increased the compactness of the dimer of extracellular domain of syndecan 2, but not of the dimer of syndecan 4. Our computational approach provides novel structural insights into syndecan 2 and 4 dimers and the structural role of GAGs on conformations of proteoglycan core proteins, which remains challenging to investigate experimentally, and even more challenging when attached to disordered core proteins such as those of syndecans.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"558 ","pages":"Article 109690"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0