Carbohydrate Research最新文献_第10页

Thermo-functional characterization of the carbohydrate-rich fractions of pea (CRFP) as influenced by particle size 豌豆富含碳水化合物组分（CRFP）的热功能表征受颗粒大小的影响

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-06-03 DOI: 10.1016/j.carres.2025.109562

Christopher Etti, Annamalai Manickavasagan

{"title":"Thermo-functional characterization of the carbohydrate-rich fractions of pea (CRFP) as influenced by particle size","authors":"Christopher Etti, Annamalai Manickavasagan","doi":"10.1016/j.carres.2025.109562","DOIUrl":"10.1016/j.carres.2025.109562","url":null,"abstract":"<div><div>This study examined the impact of particle size on the thermal and functional properties of the carbohydrate-rich fraction of pea (<em>CRFP</em>). Four <em>CRFP</em> samples were analyzed: >32<53 μm, >53<75 μm, >75 μm, and a whole fraction (control). The investigation focused on physical, thermal, pasting, functional, amylose, amylopectin, and retrogradation properties. The >75 μm fraction displayed a bimodal particle size distribution, while the others had a single mode. The >32 < 53 μm CRFP fraction had least initiation temperature (<em>T</em><sub><em>i</em></sub> = 45.82 ± 1.1 °C), concluding temperature (<em>T</em><sub><em>c</em></sub> = 59.22 ± 0.9 °C), enthalpy (<em>ΔH</em> = 2.94 ± 0.3 j/g) and gelatinization temperature range (<em>ΔT</em> = 20.90 ± 1.2 °C). Pasting temperatures showed no significant differences (P < 0.05), though the >75 μm fraction did not paste. Amylose content was highest in the finest fraction (>32 < 53 μm) and lowest in the coarsest fraction (>75 μm). Swelling capacity increased with particle size. All samples showed similar crystalline patterns characteristic of C-type starches, with the coarsest fraction displaying an additional crystalline peak. Raman spectroscopy revealed consistent structural features across all fractions. Overall, the particle size of <em>CRFP</em> influenced its thermo-functional properties, offering a promising approach for its industrial applications.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"555 ","pages":"Article 109562"},"PeriodicalIF":2.4,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advancements in glycopolymer-based sustainable biomaterials for biomedical sensing 基于糖共聚物的生物医学传感可持续生物材料的最新进展

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-05-31 DOI: 10.1016/j.carres.2025.109561

Himshikha Malviya, Seema Thapa, Jay Singh

{"title":"Recent advancements in glycopolymer-based sustainable biomaterials for biomedical sensing","authors":"Himshikha Malviya, Seema Thapa, Jay Singh","doi":"10.1016/j.carres.2025.109561","DOIUrl":"10.1016/j.carres.2025.109561","url":null,"abstract":"<div><div>Glycopolymer-based biomaterials have appeared as a reliable and adaptable framework for biomedical sensing applications by integrating the bioactivity of carbohydrate moieties with the structural variety of synthetic polymers. These substances enable precise interactions with the biomolecules, such as proteins, enzymes, and pathogens, by resembling the natural glycoconjugates. The recent advancements in glycopolymer synthesis have led us to design highly advanced and functional biomaterials. These innovations include the assimilation of the glycopolymers into nanostructures, conductive matrices, and hydrogels that significantly enhance the sensor's selectivity, stability, and sensitivity. This review highlights cutting-edge developments in glycopolymer-based biosensors for detecting critical biomarkers, such as glucose, proteins, and disease-associated antigens. Glycopolymers are also being studied for their integration into sophisticated sensing platforms, including microfluidic systems and nanostructured surfaces. Recent breakthroughs have concentrated on the development of glycopolymers employing biodegradable and bio-based monomers using green polymerization processes. These advancements have improved the environmental friendliness and biocompatibility of glycopolymers, making them more appealing for long-term biomedical sensor applications. Also, these developments demonstrate the revolutionary potential of glycopolymer-based biomaterials in addressing important healthcare issues.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"555 ","pages":"Article 109561"},"PeriodicalIF":2.4,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation, structural identification, and in vitro activity study of polysaccharides and derivatives from Polygonatum hunanense 湖南黄精多糖及其衍生物的制备、结构鉴定及体外活性研究

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-05-31 DOI: 10.1016/j.carres.2025.109550

Runcheng Zhang , Jun Zhou , Ruiding Wen , Xia Qin , Chensi Tan , Xudong Zhou , Jing Liu , Yu Ge , Zhifeng Zhang , Wei Wang , Limin Gong

{"title":"Preparation, structural identification, and in vitro activity study of polysaccharides and derivatives from Polygonatum hunanense","authors":"Runcheng Zhang , Jun Zhou , Ruiding Wen , Xia Qin , Chensi Tan , Xudong Zhou , Jing Liu , Yu Ge , Zhifeng Zhang , Wei Wang , Limin Gong","doi":"10.1016/j.carres.2025.109550","DOIUrl":"10.1016/j.carres.2025.109550","url":null,"abstract":"<div><div>Polysaccharides are one of the main active components of <em>Polygonatum hunanense</em> (PHP). This study extracted polysaccharides from the roots of PHP using water extraction and ethanol precipitation methods. Additionally, PHP was isolated and purified through various techniques, particularly through Diethylaminoethyl-52 (DEAE-52) cellulose and Sephadex G-100 chromatography columns. Structural analysis revealed that PHP is composed of 94.9 % fructose and 5.1 % glucose, with a molecular weight of 6.01 kDa. Moreover, the main chain of PHP residues linkage was found to be β-D-Fru<em>f</em>-(2 → 6)-D-Glc<em>p</em>-(1 → 2)-β-D-Fru<em>f</em>-(6,1 → 2)-β-D-Fru<em>f</em>-(1 → 2)-β-D-Fru<em>f</em>. Branch chain analysis revealed the presence of →2)-β-D-Fru<em>f</em>-(1, 6 → 2)-β-D-Fru<em>f</em>-(1 → 2)-β-D-Fru<em>f</em>. Subsequently, PHP was chemically modified, yielding acetylated polysaccharides (Ac-PHP) and sulfated polysaccharides (Su-PHP). The identification results from Fourier-transform infrared spectroscopy and <sup>13</sup>C nuclear magnetic resonance spectra indicated the successful derivatization of the polysaccharides. <em>In vitro</em> bioactivity experiments demonstrated that PHP, Ac-PHP, and Su-PHP can induce cell apoptosis and affect the cell cycle, leading to cytotoxicity toward tumor cells. Compared to PHP, Ac-PHP and Su-PHP exhibited enhanced effects in all groups. These results suggest that PHP might be a promising candidate and supplement for cancer treatment, while the acetylated and sulfated PHP polysaccharides may enhance their antitumor activity by altering their structures.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"555 ","pages":"Article 109550"},"PeriodicalIF":2.4,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144204631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detailed insights into the oligo- and polymeric products formed by three recombinant dextransucrases 详细了解低聚物和聚合物产品形成的三个重组右旋蔗糖酶

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-05-30 DOI: 10.1016/j.carres.2025.109559

Oliver Müller, Daniel Wefers

{"title":"Detailed insights into the oligo- and polymeric products formed by three recombinant dextransucrases","authors":"Oliver Müller, Daniel Wefers","doi":"10.1016/j.carres.2025.109559","DOIUrl":"10.1016/j.carres.2025.109559","url":null,"abstract":"<div><div>The water-soluble exopolysaccharide dextran can be produced from sucrose by various dextransucrases derived from lactic acid bacteria. Many of these enzymes and their corresponding dextrans have been described, but the initially formed products and the compounds which are elongated to dextrans have not been characterized in detail yet. Therefore, we studied the oligo- and polymeric products formed by recombinant dextransucrases from <em>Ligilactobacillus animalis</em> TMW 1.971 (LaniDSΔN), <em>Limosilactobacillus reuteri</em> TMW 1.106 (LreuDSΔN), and <em>Steptococcus salivarius</em> DSM 20560 (SSAL4550) from 0.25 M and 1.5 M sucrose solutions (with and without 1 M glucose) in detail. After incubation of the sucrose solutions, the enzymes mainly elongated sucrose with 1,6-linked glucose units. Erlose and leucrose were identified as additional low molecular weight compounds in the sucrose solutions, whereas glucose addition led to the predominant abundance of isomalto-oligosaccharides and kojibiose. HPSEC analysis demonstrated that glucose addition also influenced the molecular weight of the dextrans produced by LaniDSΔN and SSAL4550. To evaluate which products were used as an acceptor molecule for polysaccharide formation, the products obtained from <em>endo</em>-dextranase hydrolysis of borohydride reduced dextrans were isolated, characterized, and quantified. The quantification of glucitol-containing and theanderose-containing low molecular weight products demonstrated that sucrose and glucose are used as acceptors for dextran formation, whereas erlose or kojibiose are not elongated. At high glucose concentrations, glucose is mostly found at the non-reducing end of the dextran chain. Altogether, our findings provide detailed insights into the course of dextran formation by different dextransucrases.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"555 ","pages":"Article 109559"},"PeriodicalIF":2.4,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances on the syntheses of oligosaccharides and the corresponding glycoconjugates towards development of vaccine candidates against invasive Neisseria meningitidis strains 低聚糖合成及其糖缀合物在开发抗侵袭性脑膜炎奈瑟菌候选疫苗方面的最新进展

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-05-29 DOI: 10.1016/j.carres.2025.109545

Nabamita Basu , Mana Mohan Mukherjee , Rina Ghosh

{"title":"Recent advances on the syntheses of oligosaccharides and the corresponding glycoconjugates towards development of vaccine candidates against invasive Neisseria meningitidis strains","authors":"Nabamita Basu , Mana Mohan Mukherjee , Rina Ghosh","doi":"10.1016/j.carres.2025.109545","DOIUrl":"10.1016/j.carres.2025.109545","url":null,"abstract":"<div><h3>Neisseria meningitidis</h3><div>(Men) is one of the reasons of meningitis and sepsis worldwide. Mainly affected groups are children below five years and adolescents. Out of thirteen different Men-serogroups, disease causing invasive ones are the following six groups: A, B, C, W, X and Y. Epidemics and outbreaks by Men-strains are also reported to occur in several countries around the world. Based on the capsular polysaccharides of Men-strains (A, C, W and Y) conjugated with carrier proteins [Diphtheria toxoid (DT), CRM197 (a non-toxic mutant of DT) or Tetanus toxoid (TT)], several mono- and multi-valent vaccines were developed and licensed, which are still being used. Vaccines in the market against serogroup B are derived from MenB-proteins. Apart from these, several synthetic or semi-synthetic glycoconjugate-vaccine candidates against the disease-causing Men-serogroups are still under development or under pre-clinical or clinical trials. This minireview will encompass an overview mainly of the chemical syntheses of such semi-synthetic or synthetic glycoconjugates as Men-vaccine candidates.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"555 ","pages":"Article 109545"},"PeriodicalIF":2.4,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144204630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis and performance optimization of dual-modified chitosan Derivatives: Effects of novel zwitterionic and quaternary ammonium groups on antibacterial, antioxidant, and biocompatibility properties 双改性壳聚糖衍生物的合成及性能优化：新型两性离子和季铵基团对抗菌、抗氧化和生物相容性的影响

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-05-28 DOI: 10.1016/j.carres.2025.109556

Yonggang Peng , Ying Yu , Zhongwen Su , Yujing Zhong , Yikai Chen , Yangfan Mao , Sekar Vijayakumar , Meihua Xin , Mingchun Li

{"title":"Synthesis and performance optimization of dual-modified chitosan Derivatives: Effects of novel zwitterionic and quaternary ammonium groups on antibacterial, antioxidant, and biocompatibility properties","authors":"Yonggang Peng , Ying Yu , Zhongwen Su , Yujing Zhong , Yikai Chen , Yangfan Mao , Sekar Vijayakumar , Meihua Xin , Mingchun Li","doi":"10.1016/j.carres.2025.109556","DOIUrl":"10.1016/j.carres.2025.109556","url":null,"abstract":"<div><div>In this study, a series of halogenated zwitterionic–quaternary ammonium chitosan derivatives were synthesized through a dual-modification strategy. Their structures were confirmed by Fourier-transform infrared spectroscopy (FTIR), proton nuclear magnetic resonance (<sup>1</sup>H NMR), thermogravimetric analysis (TGA), and X-ray diffraction (XRD) analyses. Antibacterial assays demonstrated that these derivatives possess broad-spectrum activity against <em>Escherichia coli</em> and <em>Staphylococcus aureus</em>, with favorable MIC, MBC, and inhibition rate values. Antioxidant evaluations revealed improved radical-scavenging capacity compared to native chitosan. Furthermore, CCK-8 cytotoxicity and hemolysis tests confirmed their excellent biocompatibility, with no detectable toxicity. Collectively, these derivatives exhibit promising antibacterial, antioxidant, and biosafety properties, underscoring their broad potential for applications in food preservation, biomedical materials, and cosmetics.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"554 ","pages":"Article 109556"},"PeriodicalIF":2.4,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144185137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary fiber modification: A comparative study of physical, chemical, biological, combined technologies and bioactive impact on food applications 膳食纤维改性：物理、化学、生物、复合技术及其对食品应用的生物活性影响的比较研究

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-05-27 DOI: 10.1016/j.carres.2025.109558

Tapasya Kumari , Sai Babu , Kshirod Kumar Bag , Amit Baran Das , Sankar Chandra Deka

{"title":"Dietary fiber modification: A comparative study of physical, chemical, biological, combined technologies and bioactive impact on food applications","authors":"Tapasya Kumari , Sai Babu , Kshirod Kumar Bag , Amit Baran Das , Sankar Chandra Deka","doi":"10.1016/j.carres.2025.109558","DOIUrl":"10.1016/j.carres.2025.109558","url":null,"abstract":"<div><div>Daily consumption of a vast variety of plants leads to the direct disposal of a huge number of by-products, one of which is dietary fiber (DF), which are potentially advantageous bioactive compounds, in the ashcan. Nutritionists regard DF as the seventh most significant nutrient for humans. Among its many health advantages are enhanced gut flora, decreased risk of obesity and cardiovascular disorder and many more. As a result, research into plant DF modification to get high production and quality DFs (both soluble and insoluble dietary fiber) has long been a hot issue in the realm of functional food processing.The modification approaches (physical, chemical, biological, and combination) for DFs that have been studied over the past ten years are reviewed in this article, along with the varying impacts that these methods have had on the functional characteristics of DFs. The corelative analysis, its application in different food systems, and prospects of various DF approaches are explored. This review may help in determining the best DF modification approach and achieving greater industrialization of DF modification. Moreover, the application of modified DF in the food system also improves dietary fiber supplementation in the diet.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"554 ","pages":"Article 109558"},"PeriodicalIF":2.4,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144177747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced experimental set-up for HPLC-based automated synthesis and purification of carbohydrate building blocks 基于高效液相色谱的碳水化合物自动合成和纯化的强化实验装置

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-05-27 DOI: 10.1016/j.carres.2025.109554

Gustavo A. Kashiwagi, Alexei V. Demchenko

引用次数: 0

Electrochemical studies of Ruthenium (III) complexes derived from chitosan Schiff base ligands 壳聚糖席夫碱配体钌（III）配合物的电化学研究

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-05-26 DOI: 10.1016/j.carres.2025.109557

T. Vadivel , S. Kulathooran , S. Chandrasekaran , S. Ilayaraja , R. Benjamin

{"title":"Electrochemical studies of Ruthenium (III) complexes derived from chitosan Schiff base ligands","authors":"T. Vadivel , S. Kulathooran , S. Chandrasekaran , S. Ilayaraja , R. Benjamin","doi":"10.1016/j.carres.2025.109557","DOIUrl":"10.1016/j.carres.2025.109557","url":null,"abstract":"<div><div>The biocompatible nature of the chitosan biopolymer enables it to facilitate a wide range of organic transformations including chemical modification with some carbonyl compounds. Transition metal complexes namely [Ru(CS)4hy3mbd)(H<sub>2</sub>O)<sub>2</sub>].Cl<sub>2,</sub> [Ru(CS)2hybd)(H<sub>2</sub>O)<sub>2</sub>].Cl<sub>2</sub>, and [Ru(CS)2hy3mbd)(H<sub>2</sub>O)<sub>2</sub>].Cl<sub>2</sub> were synthesized by a complexation of bidentate Schiff base ligands which contains nitrogen and oxygen donor atoms. The nitrogen atom of azomethine linkage and the oxygen atom of aromatic aldehydes are the active sites for complexation reactions to form stable coordination complexes with ruthenium metal ions. The electronic transitions of ruthenium (III) complexes were studied by UV–Vis spectroscopy. The functional groups analysis were performed with FT-IR spectrum. The electronic structure and hyperfine splitting pattern of the metal complexes were discussed with ESR spectral data. The proton environment of the complex molecules were studied by 1H NMR spectroscopy. The stable complexes have shown a good crystalline nature which are characterized by powder XRD. Thermal stability of complexes was studied by Thermo-gravimetry analysis. The electrochemical property of ruthenium metal complexes were discussed with cyclic voltammogram and this study helps to distinguish reversible, quasi reversible redox properties of the metal complexes derived from biopolymer based Schiff base ligands.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"554 ","pages":"Article 109557"},"PeriodicalIF":2.4,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterization of halotolerant multifunctional GH6 endoglucanases ZFEG1605 and ZFEG1663 from Mt. Everest soil metagenome 珠穆朗玛峰土壤宏基因组GH6耐盐多功能内切葡聚糖酶ZFEG1605和ZFEG1663的鉴定与表征

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-05-24 DOI: 10.1016/j.carres.2025.109549

Justice K. Rono , Qingyun Zhang , Yong He , Leqi Zhao , Shaochen Wang , Yunbin Lyu , Chunyang Li , Anastasiah N. Ngigi , Zhi Min Yang , Zhiyang Feng

{"title":"Identification and characterization of halotolerant multifunctional GH6 endoglucanases ZFEG1605 and ZFEG1663 from Mt. Everest soil metagenome","authors":"Justice K. Rono , Qingyun Zhang , Yong He , Leqi Zhao , Shaochen Wang , Yunbin Lyu , Chunyang Li , Anastasiah N. Ngigi , Zhi Min Yang , Zhiyang Feng","doi":"10.1016/j.carres.2025.109549","DOIUrl":"10.1016/j.carres.2025.109549","url":null,"abstract":"<div><div>Environmental microorganisms express enzymes with unique hydrolytic activity, stability, and kinetic parameters, which are of great interest for biotechnological applications. In this study, two novel endoglucanases, ZFEG1605 and ZFEG1663, were cloned from Mt. Everest soil metagenomic library, heterologously expressed in <em>E. coli</em> BL21(DE3), and characterized. Both enzymes exhibited high activity on konjac glucommanan (KG) and sodium carboxymethylcellulose (CMC), while ZFEG1605 also exhibited activity towards guar gum (GG). The optimal pH for both enzymes was slightly shifted toward acidic range (pH 5/6). The optimal reaction temperatures for ZFEG1605 and ZFEG1663 were 50 and 40 °C, respectively. ZFEG1605 was more thermostable than ZFEG1663 as it remained stable up to 50 °C, compared to 40 °C for ZFEG1663. Both enzymes showed broad pH stability, although they retained more mannanase activity than CMCase activity within the same pH range. The endoglucanases exhibited remarkable salt tolerance, retaining over 70 % of their enzymatic activity in the presence of 2.5 M NaCl. The purified enzymes hydrolyzed alkali-pretreated rice straw to release reducing sugars, demonstrating their potential usage for biomass saccharification.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"554 ","pages":"Article 109549"},"PeriodicalIF":2.4,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0