Carbohydrate Research最新文献_第9页

Design, synthesis, and application of triazole-embedded maltose-neopentyl-glycol (TMNG) amphiphiles for membrane protein studies 三唑包埋麦芽糖-新戊二醇（TMNG）两亲体膜蛋白研究的设计、合成和应用

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-06-14 DOI: 10.1016/j.carres.2025.109560

Hongming Cai , Xinyu Wu , Shixin Jin , Yubin Liu , Xuyuan Liu , Kuaile Lin , Ning Ding

引用次数: 0

Unravelling the transglycosylation mechanism of Aspergillus oryzae β-galactosidase: The role of acceptor structure 米曲霉β-半乳糖苷酶转糖基化机制的揭示：受体结构的作用

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-06-14 DOI: 10.1016/j.carres.2025.109579

Cecilia Porciuncula González , Carolina Fontana , Margot Paulino , Cecilia Giacomini , Gabriela Irazoqui

{"title":"Unravelling the transglycosylation mechanism of Aspergillus oryzae β-galactosidase: The role of acceptor structure","authors":"Cecilia Porciuncula González , Carolina Fontana , Margot Paulino , Cecilia Giacomini , Gabriela Irazoqui","doi":"10.1016/j.carres.2025.109579","DOIUrl":"10.1016/j.carres.2025.109579","url":null,"abstract":"<div><div>β-Galactosidases catalyse the hydrolysis of β-<span>d</span>-galactosides and, in the presence of nucleophiles other than water, can perform transgalactosylation. This study explores the transgalactosylation activity of <em>Aspergillus oryzae</em> β-galactosidase, focusing on the impact of the acceptor structure. Lactic acid (LA) and its ethyl ester (EL) were evaluated as acceptors through the study of the kinetics of the reaction using thin-layer chromatography. The results showed that while lactic acid is not a viable acceptor, ethyl lactate generates a transgalactosylation product, identified by NMR spectroscopy as β-<span>d</span>-galactosyl-<span>l</span>-lactate ethyl ester. High concentrations of ethyl lactate reduced product hydrolysis, suggesting enzyme inhibition. Kinetic studies using <em>o</em>-Nitrophenyl β-<span>d</span>-galactopyranoside (ONPG) as a galactosyl donor substrate confirmed a substrate inhibition mechanism by ethyl lactate, with an inhibition constant (K<sub>i</sub>) of 625 ± 56 mM, comparable to other acceptors like ethylene glycol.</div><div>Molecular docking and dynamics simulations confirmed that esterifying the acceptor carboxyl group enhances transgalactosylation efficiency. <em>In silico</em> analyses confirmed key interactions between the enzyme and the acceptor, offering valuable insights into the molecular basis of transgalactosylation. These findings enhance our understanding of β-galactosidase selectivity and underscore its potential for synthesizing galactosides with applications in biocatalysis and biotechnology.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"555 ","pages":"Article 109579"},"PeriodicalIF":2.4,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144312697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stereoselective ring contractions in glycopyranosides as key-step en route to isoiminosugars 立体选择性环收缩是合成异亚糖的关键步骤

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-06-12 DOI: 10.1016/j.carres.2025.109572

Alexander Luttenberger , Elena Spari , André Culum , Tobias Dorn , Wilhelm Festl , Roland C. Fischer , Herwig Prasch , Franziska Schmutz , Arnold E. Stütz , Martin Thonhofer , Hansjörg Weber , Patrick Weber , Tanja M. Wrodnigg

引用次数: 0

Step-wise and one-pot syntheses of the trisaccharide repeating unit of Proteus penneri 71 O-antigen penneri变形杆菌71 o型抗原三糖重复单元的一步法一锅法合成

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-06-12 DOI: 10.1016/j.carres.2025.109581

Tanmoy Halder, Sunil K. Yadav, Somnath Yadav

引用次数: 0

Polysaccharides coated on mesoporous silica nanoparticles inhibit the inflammatory response for the treatment of sepsis 包裹在介孔二氧化硅纳米颗粒上的多糖抑制了脓毒症治疗的炎症反应

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-06-12 DOI: 10.1016/j.carres.2025.109580

Sha Xiong , Yajie Jia , Xiaohui Liang

{"title":"Polysaccharides coated on mesoporous silica nanoparticles inhibit the inflammatory response for the treatment of sepsis","authors":"Sha Xiong , Yajie Jia , Xiaohui Liang","doi":"10.1016/j.carres.2025.109580","DOIUrl":"10.1016/j.carres.2025.109580","url":null,"abstract":"<div><div>To address the challenges of low antibiotic delivery efficiency and complex inflammatory microenvironments in sepsis therapy, we developed a multifunctional fluorescent nanoplatform, 1-PGA-2@MSN@Meropenem, for targeted drug delivery and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-responsive detection. This system employs mesoporous silica nanoparticles (MSN) as carriers, coated with polygalacturonic acid (PGA) for enhanced stability, and functionalized with a fluorescent small molecule (Compound 1) and a natural antimicrobial agent (Compound 2). Comprehensive characterization confirmed its mesoporous structure (average pore size ∼3.4 nm), large surface area (216.4 m<sup>2</sup>/g), and successful drug loading and surface modification (FT-IR, PXRD). Fluorescence analysis revealed a strong emission at 670 nm under 390 nm excitation with a large Stokes shift (>270 nm), offering excellent signal-to-noise ratio and imaging potential. The probe exhibited a linear fluorescence response to H<sub>2</sub>O<sub>2</sub> over 0–40 μM (R<sup>2</sup> = 0.9893), with a high association constant (K<sub>a</sub> = 1.38 × 10<sup>5</sup> M<sup>−1</sup>) and outstanding selectivity. Furthermore, in an LPS-induced THP-1 macrophage inflammation model, the meropenem-loaded nanoparticles significantly downregulated pro-inflammatory cytokines TNF-α and IL-1β at the mRNA level, indicating their potential to mitigate sepsis-associated inflammation by suppressing macrophage-mediated immune responses. This work provides a promising strategy integrating drug delivery, biosensing, and immunomodulation for advanced sepsis management.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"555 ","pages":"Article 109580"},"PeriodicalIF":2.4,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144312696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growing impact of ‘Click chemistry’ inspired glycohybrid 1,2,3-Triazoles in organic synthesis “Click化学”对1,2,3-三氮杂化糖在有机合成中的影响越来越大

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-06-09 DOI: 10.1016/j.carres.2025.109571

Sumit K. Singh , Anindra Sharma , Nidhi Mishra , Vinod K. Tiwari

引用次数: 0

Enzymatic synthesis of xylosides and xylobiosides featuring aromatic aglycones 含芳香苷元的木糖苷和木糖生物苷的酶法合成

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-06-09 DOI: 10.1016/j.carres.2025.109573

Charlotte Brusa , Murielle Muzard , Emelyne Jolly , Caroline Rémond , Richard Plantier-Royon

引用次数: 0

Structural characterization of the polysaccharide produced by Lactobacillus helveticus CNRZ32 and assignment of the function of genes involved in its biosynthesis helveticus乳杆菌CNRZ32产多糖的结构特征及其生物合成相关基因功能的定位

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-06-07 DOI: 10.1016/j.carres.2025.109577

Marie-Rose Van Calsteren , Fleur Gagnon , Nancy Guertin , Christophe Gilbert

{"title":"Structural characterization of the polysaccharide produced by Lactobacillus helveticus CNRZ32 and assignment of the function of genes involved in its biosynthesis","authors":"Marie-Rose Van Calsteren , Fleur Gagnon , Nancy Guertin , Christophe Gilbert","doi":"10.1016/j.carres.2025.109577","DOIUrl":"10.1016/j.carres.2025.109577","url":null,"abstract":"<div><div>Strain CNRZ32 of <em>Lactobacillus helveticus</em> was grown in chemically defined medium or milk, and its exopolysaccharide (EPS) was isolated from the supernatant or the whole culture and purified by TCA and acetone precipitations and solvent extractions. Purity was checked by gel permeation chromatography. The molecular mass determined by size-exclusion chromatography was 6.4 × 10<sup>5</sup> g/mol. Structural elucidation was performed by chemical, chromatographic, and spectroscopic methods. Monosaccharide and configuration analyses gave the following sugar composition: <span>d</span>-Gal, 2; <span>d</span>-Glc, 1; <span>d</span>-GlcNAc, 1; <span>l</span>-Rha, 3. Methylation analysis indicated the presence of terminal and 3,6-disubstituted Gal, 4-substituted Glc, 3,4,6-trisubstituted GlcNAc, as well as terminal and 2,4-disubstituted Rha. A phosphoethanolamine substituent was detected by NMR spectroscopy. On the basis of one- and two-dimensional homo- and heteronuclear NMR data, the structure of the EPS was consistent with a multibranched heptasaccharide repeating unit with the following sequence: {4[Rhaα-3]GlcNAc6<em>P</em>EtNβ-3[Galβ-6]Galα-4[Rhaα-2]Rhaβ-4Glcβ-}<sub><em>n</em></sub>. Electrospray MS and MS/MS data are given for oligosaccharides produced after deacetylation, deamination, and reduction. A correlation between the EPS sequence and genes of the <em>L. helveticus</em> CNRZ32 <em>eps</em> locus encoding putative enzymes responsible for the biosynthesis of the repeating unit is proposed. The structure is compared to that of EPSs produced by other <em>L. helveticus</em> strains.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"555 ","pages":"Article 109577"},"PeriodicalIF":2.4,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144481786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drug-loaded composite materials based on polysaccharide carriers for alleviating myocardial cell damage 以多糖为载体的载药复合材料减轻心肌细胞损伤

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-06-06 DOI: 10.1016/j.carres.2025.109574

Lingling Sun , Lifeng Zhu , Xiaoci Li , Weimin Jiang

{"title":"Drug-loaded composite materials based on polysaccharide carriers for alleviating myocardial cell damage","authors":"Lingling Sun , Lifeng Zhu , Xiaoci Li , Weimin Jiang","doi":"10.1016/j.carres.2025.109574","DOIUrl":"10.1016/j.carres.2025.109574","url":null,"abstract":"<div><div>Heart failure, a leading cause of mortality among cardiovascular disease patients, is primarily a consequence of chronic heart failure (CHF) and myocardial tissue damage associated with apoptosis. Leveraging the pharmacological success of Dapagliflozin, an SGLT-2 inhibitor known for reducing glucose reabsorption and its preventative efficacy against cardiovascular diseases, we have engineered and synthesized a novel derivative, compound 2, to enhance therapeutic outcomes. Furthermore, we utilized chitosan (CS) as a carrier matrix and integrated a bioactive compound, compound 1, synthesized from actinomycetes, to develop an antimicrobial drug delivery material, CS-1, designated for the administration of compound 2 (CS-1@2), which exhibits pH-responsive antimicrobial effects. The physicochemical properties, in vitro biological characteristics, and bioactivity of CS-1@2 were rigorously evaluated. Results indicated that CS-1 possesses a porous structure, facilitating accelerated release of compound 2 in mildly acidic conditions. In vitro cellular assays were conducted, establishing a CHF model using doxorubicin-induced AC16 cardiomyocytes. This study aims to elucidate the mechanisms through which CS-1@2 mitigates doxorubicin-induced damage in AC16 myocardial cells, highlighting its potential in CHF management. This study lays a promising foundation for targeted therapy of chronic heart failure (CHF) and promotes its translational application in personalized cardiovascular medicine.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"555 ","pages":"Article 109574"},"PeriodicalIF":2.4,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144242360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Total synthesis of the linker-armed tetrasaccharide repeating unit of the O-polysaccharide from E. coli O50 大肠杆菌O50中o -多糖连接臂四糖重复单元的全合成

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-06-04 DOI: 10.1016/j.carres.2025.109563

Subrata Das, Balaram Mukhopadhyay

引用次数: 0