Carbohydrate Research最新文献_第8页

Optimization, characterization and application of chitooligosaccharide-urea conjugate for wheat seed priming: A sustainable approach 壳寡糖-尿素偶联物的优化、表征及应用：一种可持续的方法

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2026-01-01 Epub Date: 2025-10-30 DOI: 10.1016/j.carres.2025.109722

Surbhi Sahewalla , Sonam Sihag , Yamini Tak , Anil Duhan , Vinod Saharan , Ajay Pal

{"title":"Optimization, characterization and application of chitooligosaccharide-urea conjugate for wheat seed priming: A sustainable approach","authors":"Surbhi Sahewalla , Sonam Sihag , Yamini Tak , Anil Duhan , Vinod Saharan , Ajay Pal","doi":"10.1016/j.carres.2025.109722","DOIUrl":"10.1016/j.carres.2025.109722","url":null,"abstract":"<div><div>The present study deals with the preparation of chitooligosaccharide (COS), its conjunction with urea, and its priming effect on wheat grains. Employing central composite rotatable design (CCRD) of response surface methodology (RSM) to optimize conditions for COS production resulted in COS with a reducing sugar: 4.14 mg GAH/ml and an average particle size: 232 nm. FTIR, FESEM and XRD validated its formation. The COS displayed exceptional antioxidant properties <em>via.</em> DPPH (IC<sub>50</sub>: 7.74 mg/ml), ABTS (IC<sub>50</sub>: 0.147 mg/ml), MCA (IC<sub>50</sub>: 0.329 mg/ml) and TAA (AO<sub>0.5</sub>: 1.76 mg/ml). It was then dopped with urea, and FTIR and NMR detailed the interaction between COS and urea, primarily owing to hydrogen bonds. On studying the effect of priming of wheat grains, an increase of 41.5 % and 22.62 % in shoot and root length of wheat seedlings was noticed with COS-urea (25–50 ppm). Fresh weight, dry weight, SVI-I and SVI-II also detailed the maximum positive trend with COS-urea followed by the maximum utilization of starch and protein. This study provides a new perspective on the application of COS-urea for enhancing the germination potential of wheat, and also for its potential use as a foliar spray, contributing towards sustainable agriculture and the environment.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"559 ","pages":"Article 109722"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145415464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipooligosaccharide architecture in Acinetobacter modestus CM11G: a non-pathogenic strain 温和不动杆菌CM11G的脂低糖结构：一种非致病性菌株。

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2026-01-01 Epub Date: 2025-11-11 DOI: 10.1016/j.carres.2025.109743

Immacolata Speciale , Luisa Sturiale , Angelo Palmigiano , Anna Notaro

{"title":"Lipooligosaccharide architecture in Acinetobacter modestus CM11G: a non-pathogenic strain","authors":"Immacolata Speciale , Luisa Sturiale , Angelo Palmigiano , Anna Notaro","doi":"10.1016/j.carres.2025.109743","DOIUrl":"10.1016/j.carres.2025.109743","url":null,"abstract":"<div><div>The <em>Acinetobacter</em> genus comprises a multitude of species that have been isolated from both environmental and clinical samples. In recent years, the scientific community has expended considerable effort in characterising the structures of the capsular polysaccharides and lipooligoaccharides (LOS) of <em>A. baumannii</em>, given the increasing mortality rate caused by this species. Comparatively little research has been undertaken for the non-pathogenic species.</div><div>In the present study, we describe the first case of isolation and identification of the LOS molecule of <em>Acinetobacter modestus</em> CM11G, a Gram-negative bacterium colonising the intestinal crypts of a healthy mouse. By combining spectroscopic and spectrometric analyses with chemical derivatisations, we were able to determine the structure of the entire LOS. The lipid A moiety is composed mainly of hepta-acylated species, a characteristic also observed in other <em>Acinetobacter</em> species. It is extended with a disaccharide of Kdo, which acts as a bridge between the lipid A and the oligosaccharide portion. Indeed, the internal Kdo residue is linked at position <em>O</em>-5 with a novel tetrasaccharide composed of βGlcN(1→2)-βGal(1→6)-αGlc(1→, where the glucose is further substituted at position <em>O</em>-4 with a terminal β-Glc. In contrast, the external Kdo residue does not undergo further substitution, contrary to what generally occurs in the LOS of <em>A. baumannii</em>.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"559 ","pages":"Article 109743"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145522983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unexpected formation of furanose form during deacetylation of pyranose glyco-oxazolines 在吡喃糖-恶唑啉去乙酰化过程中意外形成呋喃糖。

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2026-01-01 Epub Date: 2025-11-19 DOI: 10.1016/j.carres.2025.109761

Maxim A. Romanyuk, Ilya V. Myachin, Maria V. Panova, Alexander I. Zinin, Oleg R. Malyshev, Natalya G. Kolotyrkina, Leonid O. Kononov

{"title":"Unexpected formation of furanose form during deacetylation of pyranose glyco-oxazolines","authors":"Maxim A. Romanyuk, Ilya V. Myachin, Maria V. Panova, Alexander I. Zinin, Oleg R. Malyshev, Natalya G. Kolotyrkina, Leonid O. Kononov","doi":"10.1016/j.carres.2025.109761","DOIUrl":"10.1016/j.carres.2025.109761","url":null,"abstract":"<div><div>We have found that the deacetylation of <em>O</em>-acetylated pyranose glyco-oxazoline, derived from <em>N</em>-acetyl-<span>d</span>-glucosamine, using MeONa or other bases (Et<sub>3</sub>N, K<sub>2</sub>CO<sub>3</sub>) in MeOH, selectively gives the expected pyranose triol at 20 °C. In contrast, at 60 °C, a previously unknown, thermodynamically favored furanose isomer of the glyco-oxazoline triol is formed. Isomeric <em>O</em>-acetylated pyranose glyco-oxazolines, derived from <em>N</em>-acetyl-<span>d</span>-galactosamine or <em>N</em>-acetyl-<span>d</span>-mannosamine, also afford furanose isomers of the corresponding glyco-oxazoline triols upon treatment with MeONa in MeOH at 60 °C. The obtained unprotected furanose glyco-oxazoline with <em>gluco</em>-configuration was transformed into <em>O</em>-acetylated furanose <em>gluco</em>-oxazoline. The ability to prepare (un)protected <em>furanose</em> glyco-oxazolines opens a novel pathway to the underexplored furanose forms of glyco-oxazolines bearing various <em>O</em>-protective groups.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"559 ","pages":"Article 109761"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145602534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A ‘chiron’ approach to divergent synthesis of 2-C-tridecyl 1-N-iminosugar mimetics and their anticancer profile 2- c -三烷基1- n -亚糖模拟物的“chiron”方法及其抗癌特性。

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2026-01-01 Epub Date: 2025-11-13 DOI: 10.1016/j.carres.2025.109742

Monika Tvrdoňová , Peter Michalčin , Tatiana Pončáková , Yuliia Zuzak , Martina Bago Pilátová , Juraj Kuchár , Miroslava Litecká , Miroslava Martinková

引用次数: 0

Structural diversity of the OPSs isolated from the LPSs of the plant pathogenic bacteria Pectobacterium parmentieri IFB5441 and IFB5533 植物致病菌Pectobacterium parmentieri IFB5441和IFB5533的lps分离产物的结构多样性

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2026-01-01 Epub Date: 2025-11-19 DOI: 10.1016/j.carres.2025.109762

Karolina Ossowska , Natalia Kaczyńska , Agnieszka Kowalczyk , Tomasz Apanowicz , Zbigniew Kaczyński

{"title":"Structural diversity of the OPSs isolated from the LPSs of the plant pathogenic bacteria Pectobacterium parmentieri IFB5441 and IFB5533","authors":"Karolina Ossowska , Natalia Kaczyńska , Agnieszka Kowalczyk , Tomasz Apanowicz , Zbigniew Kaczyński","doi":"10.1016/j.carres.2025.109762","DOIUrl":"10.1016/j.carres.2025.109762","url":null,"abstract":"<div><div><em>Pectobacterium parmentieri</em> is a pectinolytic pathogenic bacterium causing high economic losses in plant crops. Lipopolysaccharide is one of the bacterial virulence factors that play an important role in plant colonisation and interaction with host defence systems. The chemical structure of an O-polysaccharide isolated from the lipopolysaccharide of the <em>P. parmentieri</em> IFB5441 was determined using NMR spectroscopy and chemical methods. The repeating unit of the O-polysaccharide was composed of three galactose residues and one residue of 5,7-diamino-3,5,7,9-tetradeoxy-<span>l</span>-glycero-<span>l</span>-manno-non-2-ulosonic acid:<span><figure><span><img><ol><li><span><span>Download: <span>Download high-res image (46KB)</span></span></span></li><li><span><span>Download: <span>Download full-size image</span></span></span></li></ol></span></figure></span></div><div>The chemical structure of the repeating unit of the O-polysaccharide isolated from the <em>P. parmentieri</em> IFB5533 was also determined revealing the presence of galactose, <em>N</em>-acetylgalactosamine and <em>N</em>-acetylmannosamine:<span><figure><span><img><ol><li><span><span>Download: <span>Download high-res image (39KB)</span></span></span></li><li><span><span>Download: <span>Download full-size image</span></span></span></li></ol></span></figure></span></div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"559 ","pages":"Article 109762"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145621023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification of Quillaja saponins QS-21Xyl and QS-21Api by hydrophilic interaction chromatography 亲水相互作用色谱法纯化黄芪总皂苷QS-21Xyl和QS-21Api。

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2026-01-01 Epub Date: 2025-11-08 DOI: 10.1016/j.carres.2025.109741

Shuyu Yue, Jishun Shi, Changyu Cai, Yanxiong Ke

{"title":"Purification of Quillaja saponins QS-21Xyl and QS-21Api by hydrophilic interaction chromatography","authors":"Shuyu Yue, Jishun Shi, Changyu Cai, Yanxiong Ke","doi":"10.1016/j.carres.2025.109741","DOIUrl":"10.1016/j.carres.2025.109741","url":null,"abstract":"<div><div>QS-21 is a highly effective vaccine adjuvant that is purified from the bark of the <em>Quillaja saponaria</em> tree. However, due to the complex structure of saponins and the existence of multiple isomers with similar structures, obtaining high-purity QS-21 is extremely challenging. This study describes the development of a chromatographic process for purifying QS-21<sub>xyl</sub> and QS-21<sub>Api</sub> using commercially available <em>Q. saponaria</em> bark extract as the starting material. First, the sample was purified using Polyvinyl pyrrolidone-divinylbenzene (PVP-DVB) copolymer resin to remove highly polar compounds and obtain a saponin-enriched component. Then, the fraction containing QS-21 was obtained using a C18 column. Impurities in the sample were identified using Matrix-Assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) mass spectrometry, including structurally analogous impurities such as S1, S2, S3 and S5. Analysis using an amide column in hydrophilic interaction chromatography (HILIC) mode exhibited different separation selectivity compared to analysis using a C18 column in reverse phase mode. The HILIC separation condition of QS-21<sub>xyl</sub> and QS-21<sub>Api</sub> was optimized and then the compounds were purified using a semi-preparative chromatographic column. The resulting QS-21<sub>Xyl</sub> and QS-21<sub>Api</sub> had a purity greater than 97 %. This process provides an efficient method of obtaining highly pure QS-21 isomers from <em>Q. Saponaria</em> bark extract.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"559 ","pages":"Article 109741"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145502080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbohydrate research JSCR44 poster award 碳水化合物研究JSCR44海报奖。

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2026-01-01 Epub Date: 2025-11-05 DOI: 10.1016/j.carres.2025.109735

Toshiki Nokami (Editorial Board Member)

引用次数: 0

The roles of ST3Gal1-6 in cancer: expression profiles and functional implications ST3Gal1-6在癌症中的作用：表达谱和功能意义

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2026-01-01 Epub Date: 2025-11-06 DOI: 10.1016/j.carres.2025.109740

Jierou Fan , Siqi Huang , Chenyin Cao , Ximu Jin , Yanting Su

引用次数: 0

Structure of the O-polysaccharide from the lipopolysaccharide of Vreelandella venusta strain B511 venusta菌株B511脂多糖中o -多糖的结构。

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2026-01-01 Epub Date: 2025-11-04 DOI: 10.1016/j.carres.2025.109734

Maxim S. Kokoulin , Elena N. Sigida , Vlada S. Belova , Marina S. Kuzina , Natalya S. Velichko , Vyacheslav S. Grinev , Yuliya P. Fedonenko

引用次数: 0

Photocrosslinkable carbohydrate-based hydrogels for cartilage regeneration: Current insights and future perspectives 用于软骨再生的光交联碳水化合物基水凝胶：当前的见解和未来的观点。

IF 2.5 3区化学

Carbohydrate Research Pub Date : 2026-01-01 Epub Date: 2025-10-30 DOI: 10.1016/j.carres.2025.109718

Pegah Poorkhalili, Jhamak Nourmohammadi, Niloufar Zamirinadaf

{"title":"Photocrosslinkable carbohydrate-based hydrogels for cartilage regeneration: Current insights and future perspectives","authors":"Pegah Poorkhalili, Jhamak Nourmohammadi, Niloufar Zamirinadaf","doi":"10.1016/j.carres.2025.109718","DOIUrl":"10.1016/j.carres.2025.109718","url":null,"abstract":"<div><div>Osteochondral defects, involving degeneration of articular cartilage and underlying bone, remain a major challenge in orthopedics due to the tissue's limited self-repair capacity. Traditional treatments often fail to fully restore cartilage structure and function, creating a need for advanced biomaterials to support regeneration. Hydrogels, with their high water content, biocompatibility, and tunable properties, are promising for cartilage tissue engineering. Photocrosslinkable hydrogels have gained attention for their spatiotemporal control, rapid <em>in situ</em> gelation, and ability to form stable scaffolds under light exposure. They offer tunable mechanical strength, degradation rates, and cellular compatibility, making them suitable for cartilage repair. Carbohydrate-based hydrogels, especially those mimicking the extracellular matrix (ECM), show strong potential due to their structural plasticity, bioactivity, and support of chondrocyte function. Their molecular structures, with variations in chain length and branching, allow fine-tuning of physicochemical properties for enhanced performance. This review highlights recent progress in photocrosslinkable carbohydrate-based hydrogels for cartilage regeneration, focusing on fabrication methods, crosslinking strategies, and chemical modifications such as methacrylation and acrylation. It also discusses the advantages of photopolymerization, including efficiency and low cytotoxicity, and its role in improving mechanical properties and chondrogenic potential. Finally, current challenges and future clinical prospects are addressed.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"559 ","pages":"Article 109718"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145494761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0