Carbohydrate Research最新文献_第8页

Glycans of parasitic nematodes – from glycomes to novel diagnostic tools and vaccines

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-01-22 DOI: 10.1016/j.carres.2025.109407

Shi Yan

引用次数: 0

Efficient synthesis of coumarin based triazole linked O-glycoconjugates as new bio-active glycohybrids

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-01-21 DOI: 10.1016/j.carres.2025.109395

Sunil Sharma, Ram Sagar

{"title":"Efficient synthesis of coumarin based triazole linked O-glycoconjugates as new bio-active glycohybrids","authors":"Sunil Sharma, Ram Sagar","doi":"10.1016/j.carres.2025.109395","DOIUrl":"10.1016/j.carres.2025.109395","url":null,"abstract":"<div><div>Glycohybrids are biologically significant molecules with variety of biological functions and are found as structural motifs in numerous natural products. Here, we report the synthesis of various new coumarin-based <em>O</em>-glycoconjugates as glycohybrids that are chirally enriched and bridged by 1,2,3-triazoles ring system. The1,2,3-triazoles bridging was done via CuAAC click-chemistry. Click chemistry offers several advantages, including high chemo- and regioselectivity, mild reaction conditions, easy purification, and compatibility with multiple functional groups. Two series of <em>O</em>-glycoconjugates as new glycohybrids were designed and efficiently synthesized in very good isolated yields, using <span>d</span>-glucose, <span>d</span>-galactose, <span>d</span>-mannose, <span>d</span>-arabinose, 3,4,6-tri-<em>O</em>-acetyl-D-glucal, 3,4,6-tri-<em>O</em>-acetyl-D-galactal and 3,4-di-<em>O</em>-acetyl-D-arabinal derived 1-<em>O</em>-propargylated glycosides, reacting with various 4-azidocoumarins under click-chemistry reaction conditions. The prepared new coumarin-based <em>O</em>-glycoconjugates as glycohybrids were found to possess anticancer activity against MCF-7 breast cancer cell lines at micromolar concentration.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"550 ","pages":"Article 109395"},"PeriodicalIF":2.4,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Named reaction in carbohydrate chemistry: A review

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-01-21 DOI: 10.1016/j.carres.2025.109396

Shakeel Alvi, Rashid Ali

引用次数: 0

Chloramphenicol glycoside derivative: A way to overcome its antimicrobial resistance and toxicity

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-01-21 DOI: 10.1016/j.carres.2025.109387

Mariusz Dziadas , Natalia Pachura , Anna Duda-Madej , Mateusz Garbicz , Tomasz Gębarowski , Alicia Dominguez-Martin , Magdalena Rowińska-Żyrek

引用次数: 0

Cassava starch extraction with a polygalacturonase from a wild yeast strain with disintegration activity on plant tissues

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-01-18 DOI: 10.1016/j.carres.2025.109386

Silvana A. Maidana, Ana Paula Butiuk, Luis Alberto Brumovsky, María Alicia Martos

{"title":"Cassava starch extraction with a polygalacturonase from a wild yeast strain with disintegration activity on plant tissues","authors":"Silvana A. Maidana, Ana Paula Butiuk, Luis Alberto Brumovsky, María Alicia Martos","doi":"10.1016/j.carres.2025.109386","DOIUrl":"10.1016/j.carres.2025.109386","url":null,"abstract":"<div><div>The objective of the present study was to optimize an enzymatic starch extraction process from cassava roots using a polyglacturonase (PGase) from a wild yeast strain (<em>Wickerhanomyces anomalus</em>).</div><div>The supernatant of <em>W. anomalus</em> culture, with PGase activity, was used as source of enzyme (enzymatic extract, EE). The cassava starch extractions with the EE were done at lab scale and the effects of several factors were studied by using statistical designs. Chemical and functional properties of the starch obtained (enzymatic starch, ES) and those of a commercial starch (CS), were determined.</div><div>The highest extraction yield was obtained using <em>processed</em> cassava tissues, a solid-liquid ratio of 3:6, EE with an enzyme dosage of 15 EU mL<sup>−1</sup>, at 40 °C for 5 h. Proximal analysis of the ES did not show significant differences with the CS. Swelling power and water solubility of ES increased with the temperature up to 70 °C (1.97 gg<sup>-1</sup>) and 90 °C (4.27 % w/w), respectively, similar to those of CS. Viscoamylographic profiles of ES showed a pasting temperature of 63.5 °C, a viscosity peak of 422 BU, an stability of the pasta during cooking of 202 BU and a retrograde trend of 98 BU. These values were slightly different compared to those of CS. Clarity assays determined that ES paste is considered a clear or transparent paste. Microscopic evaluation of the ES grains revealed that they were intact without any mechanical damage.</div><div>The production of cassava starch by enzyme technology seems to be an interesting alternative to the traditional method of extraction by mechanical means, resulting in a novel biotechnological process.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"550 ","pages":"Article 109386"},"PeriodicalIF":2.4,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antigenicity evaluation of the synthetic trisaccharides related to Plesiomonas shigelloides serotype O51 O-antigen containing an acetamidino group 含乙酰氨基基的志贺单胞菌O51 o型抗原相关合成三糖的抗原性评价。

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-01-16 DOI: 10.1016/j.carres.2025.109388

Chunjun Qin , Wei Han , Guangzong Tian , Xiaopeng Zou , Anna Maciejewska , Jolanta Lukasiewicz , Peter H. Seeberger , Jing Hu , Jian Yin

{"title":"Antigenicity evaluation of the synthetic trisaccharides related to Plesiomonas shigelloides serotype O51 O-antigen containing an acetamidino group","authors":"Chunjun Qin , Wei Han , Guangzong Tian , Xiaopeng Zou , Anna Maciejewska , Jolanta Lukasiewicz , Peter H. Seeberger , Jing Hu , Jian Yin","doi":"10.1016/j.carres.2025.109388","DOIUrl":"10.1016/j.carres.2025.109388","url":null,"abstract":"<div><div>Bacterial surface polysaccharides are widely decorated with diverse rare functional groups. An understanding of the role of these functional groups in immunological activity of the glycans is required for the development of carbohydrate-based treatment against bacterial infectious diseases. Here, the antigenicity evaluation of synthetic trisaccharide <strong>1</strong> related to <em>Plesiomonas shigelloides</em> serotype O51 lipopolysaccharide (LPS) O-antigen and its acetamido derivative <strong>2</strong> was achieved to elucidate the immunological importance of O-antigen structure. The rabbit antisera with high titers of IgG and IgM were collected after immunization with purified <em>P. shigelloides</em> serotype O51 LPS. Glycan microarray screening results showed that both IgG and IgM in rabbit antisera bound specifically to trisaccharides <strong>1</strong> and <strong>2</strong>, indicating that the trisaccharide repeating unit is a key antigenic epitope of <em>P. shigelloides</em> serotype O51 O-antigen, while the acetamidino group has no influence on the antigenicity. This finding provides key information for glycoconjugate vaccine design for <em>P. shigelloides</em> serotype O51.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"550 ","pages":"Article 109388"},"PeriodicalIF":2.4,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of 2-hydroxy analogues of castanospermine, 1-epi-castanospermine and their iminooctitols from sugar-derived lactam 糖源内酰胺合成2-羟基castanospermine、1-epi-castanospermine及其亚氨基糖醇。

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-01-14 DOI: 10.1016/j.carres.2025.109385

Nagaraja Ingaladal , Ravi S. Lankalapalli

引用次数: 0

Unraveling the diversity of protein-carbohydrate interfaces: Insights from a multi-scale study 揭示蛋白质-碳水化合物界面的多样性：来自多尺度研究的见解。

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-01-13 DOI: 10.1016/j.carres.2025.109377

Aria Gheeraert , Frédéric Guyon , Serge Pérez , Tatiana Galochkina

{"title":"Unraveling the diversity of protein-carbohydrate interfaces: Insights from a multi-scale study","authors":"Aria Gheeraert , Frédéric Guyon , Serge Pérez , Tatiana Galochkina","doi":"10.1016/j.carres.2025.109377","DOIUrl":"10.1016/j.carres.2025.109377","url":null,"abstract":"<div><div>Protein-carbohydrate interactions play a crucial role in numerous fundamental biological processes. Thus, description and comparison of the carbohydrate binding site (CBS) architecture is of great importance for understanding of the underlying biological mechanisms. However, traditional approaches for carbohydrate-binding protein analysis and annotation rely primarily on the sequence-based methods applied to specific protein classes. The recently released DIONYSUS database aims to fill this gap by providing tools for CBS comparison at different levels: both in terms of protein properties and classification, as well as in terms of atomistic CBS organization. In the current study, we explore DIONYSUS content using a combination of the suggested approaches in order to evaluate the diversity of the currently resolved non-covalent protein-carbohydrate interfaces at different scales. Notably, our analysis reveals evolutionary convergence of CBS in proteins with distinct folds and coming from organisms across different kingdoms of life. Furthermore, we demonstrate that a CBS structure based approach has the potential to facilitate functional annotation for the proteins with missing information in the existing databases. In particular, it provides reliable information for numerous carbohydrate-binding proteins from rapidly evolving organisms, whose analysis is particularly challenging for classical sequence-based methods.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"550 ","pages":"Article 109377"},"PeriodicalIF":2.4,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review of the latest advancements in using β-cyclodextrin-based as supramolecular catalysts for Heck reactions 综述了以β-环糊精为基础的Heck反应超分子催化剂的最新研究进展。

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-01-13 DOI: 10.1016/j.carres.2025.109381

Sara Payamifar , Majid Abdouss , Shahrzad Javanshir , Ahmad Poursattar Marjani

{"title":"A review of the latest advancements in using β-cyclodextrin-based as supramolecular catalysts for Heck reactions","authors":"Sara Payamifar , Majid Abdouss , Shahrzad Javanshir , Ahmad Poursattar Marjani","doi":"10.1016/j.carres.2025.109381","DOIUrl":"10.1016/j.carres.2025.109381","url":null,"abstract":"<div><div>The Heck reaction is one of the most well-known C–C (carbon-carbon) coupling reactions and was identified with the Nobel Prize in Chemistry in 2010. These reactions have been broadly utilized to prepare a different spectrum of heterocycles with applications in agrochemical and pharmaceutical industries. These reactions are commonly catalyzed by palladium due to its tolerance and expansive variousness of functional groups, which bears a remarkable power in creating C–C bonds. Carbohydrate chemistry is a significant and crucial part of the organic chemistry field. Among existing carbohydrates, β-cyclodextrins (β-CDs) are natural cyclic oligosaccharides that contain glucose monomers and have played an active role in nearly every industrial and scientific field, attention and interest in them never aged. CDs can be utilized as a beneficial catalyst in numerous aqueous organic conversions. Their derivatives have demonstrated remarkable activity in water-mediated organic synthesis and are currently of continuing interest to multiple scientists in different fields. Being non-toxic, accessible, biodegradable, readily functionalized, low-cost, renewable, and producible in large-scale amounts are several of the benefits of CDs that make them a suitable nominee for catalyst applications. Different β-CDs catalysts have recently been widely employed for various C–C bond coupling reactions like the Heck coupling. Here, we review the latest advancements in using CD-based catalysts for Heck reactions.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"550 ","pages":"Article 109381"},"PeriodicalIF":2.4,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Injectable vancomycin loaded hyaluronic acid-chitosan hydrogel for the treatment of Staphylococcus aureus septic arthritis 万古霉素负载透明质酸-壳聚糖水凝胶注射治疗金黄色葡萄球菌脓毒性关节炎。

IF 2.4 3区化学

Carbohydrate Research Pub Date : 2025-01-13 DOI: 10.1016/j.carres.2025.109384

K. Kumaran , Anil Kumar Vasudevan , R. Jayakumar , Raja Biswas

{"title":"Injectable vancomycin loaded hyaluronic acid-chitosan hydrogel for the treatment of Staphylococcus aureus septic arthritis","authors":"K. Kumaran , Anil Kumar Vasudevan , R. Jayakumar , Raja Biswas","doi":"10.1016/j.carres.2025.109384","DOIUrl":"10.1016/j.carres.2025.109384","url":null,"abstract":"<div><div>Staphylococcus aureus (<em>S. aureus</em>) is a Gram positive opportunistic pathogen and a major cause for bacterial septic arthritis. Vancomycin is the preferred antibiotic for the treatment of methicillin resistance S. aureus septic arthritis. Patients undergoing vancomycin treatment need to be hospitalized and their serum vancomycin level should be monitored, as increase in vancomycin concentration in serum may lead to hepatotoxicity. To overcome vancomycin mediated cytotoxicity, we have prepared a local injectable delivery system by incorporating vancomycin into hyaluronic acid (HA)-chitosan (van-HA-chitosan) hydrogel. The prepared van-HA-chitosan hydrogel was characterized using Fourier Transform Infrared Spectroscopy (FTIR) and rheometer. The van-HA-chitosan hydrogel is injectable, has shear thinning behaviour; and is hemo- and cyto-compatible. <em>In vitro</em> drug release assay showed that 95 % of vancomycin was released from the hydrogel in 8 days. Under <em>in vitro</em> conditions the load of <em>S. aureus</em> decreased from 6.4 Log<sub>10</sub> CFU/ml to 3.5 Log<sub>10</sub> CFU/ml when treated with van-HA-chitosan hydrogel for 6 h. Significant decrease in bacterial counts was observed when <em>S. aureus</em> infected synovial fluid and bone samples were treated with van-HA-chitosan hydrogel. Our results suggest that the prepared van-HA-chitosan could be used for the treatment of septic arthritis caused by <em>S. aureus</em>.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"550 ","pages":"Article 109384"},"PeriodicalIF":2.4,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0