Chunjun Qin, Wei Han, Guangzong Tian, Xiaopeng Zou, Anna Maciejewska, Jolanta Lukasiewicz, Peter H Seeberger, Jing Hu, Jian Yin
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引用次数: 0
摘要
细菌表面多糖广泛地装饰着各种罕见的官能团。了解这些官能团在聚糖免疫活性中的作用是开发以碳水化合物为基础的治疗细菌感染性疾病的必要条件。本研究通过对志贺氏单胞菌血清型O51脂多糖（LPS） o -抗原及其乙酰氨基衍生物2相关的合成三糖1的抗原性评价，阐明o -抗原结构的免疫学重要性。用纯化的O51型志贺氏杆菌LPS免疫兔，获得高滴度IgG和IgM的抗血清。糖基芯片筛选结果显示，兔抗血清中IgG和IgM均与三糖1和2特异性结合，表明三糖重复单元是志贺氏杆菌O51 o型抗原的关键抗原表位，而乙酰氨基基对其抗原性没有影响。这一发现为O51型志贺氏杆菌糖结合疫苗的设计提供了关键信息。
Antigenicity evaluation of the synthetic trisaccharides related to Plesiomonas shigelloides serotype O51 O-antigen containing an acetamidino group.
Bacterial surface polysaccharides are widely decorated with diverse rare functional groups. An understanding of the role of these functional groups in immunological activity of the glycans is required for the development of carbohydrate-based treatment against bacterial infectious diseases. Here, the antigenicity evaluation of synthetic trisaccharide 1 related to Plesiomonas shigelloides serotype O51 lipopolysaccharide (LPS) O-antigen and its acetamido derivative 2 was achieved to elucidate the immunological importance of O-antigen structure. The rabbit antisera with high titers of IgG and IgM were collected after immunization with purified P. shigelloides serotype O51 LPS. Glycan microarray screening results showed that both IgG and IgM in rabbit antisera bound specifically to trisaccharides 1 and 2, indicating that the trisaccharide repeating unit is a key antigenic epitope of P. shigelloides serotype O51 O-antigen, while the acetamidino group has no influence on the antigenicity. This finding provides key information for glycoconjugate vaccine design for P. shigelloides serotype O51.
期刊介绍：
Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects.
Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence.
Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".
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