Current stem cell research & therapy最新文献

{"title":"Global Trends and Knowledge Structure in HIV Stem Cell Research: A CiteSpace Analysis of Two Decades of Scientific Literature.","authors":"Xi Chen, Yu Lai","doi":"10.2174/011574888X392519251128043424","DOIUrl":"https://doi.org/10.2174/011574888X392519251128043424","url":null,"abstract":"<p><strong>Introduction: </strong>Numerous studies on HIV (human immunodeficiency virus) in stem cells have been published over the last two decades. However, no comprehensive bibliometric analysis or visualization of this field currently exists. This study aims to provide a detailed and unbiased review of the knowledge structure, research hotspots, and emerging trends in stem cell therapy-related research for HIV. By mapping publication trends, collaboration networks, and topical evolution, this work fills a critical gap and offers guidance for future functional cure strategies.</p><p><strong>Methods: </strong>Bibliometric data from 2004 to 2023 were retrieved from the Web of Science Core Collection database. CiteSpace was used to analyze annual scientific output, countries/regions, institutions, authors, journals, references, and keywords.</p><p><strong>Results: </strong>A total of 2,868 publications were included, showing a general trend of increasing yet fluctuating annual output. The United States led in both publications and influence, while author collaboration remained relatively limited. Key research topics included acquired immune deficiency syndrome cure, lentivirus, HIV carcinogenicity, CCR5, and CXCR4. More recently, major active areas of investigation have focused on \"extracellular vesicles,\" \"nucleases,\" \"viral libraries,\" and \"microglia.\"</p><p><strong>Discussion: </strong>HIV stem cell research has progressed from descriptive studies to mechanism-driven approaches, emphasizing gene editing and immune modulation. Integrating stem cell transplantation with technologies such as CRISPR/Cas9 presents a promising strategy to eliminate viral reservoirs and sustain remission. Nevertheless, stronger international collaboration and translational research are essential to advance clinical applications.</p><p><strong>Conclusion: </strong>This comprehensive bibliometric overview provides clear insights into the development, research hotspots, and trends in HIV stem cell therapy. Further investigation into the molecular mechanisms of infection and strategies for functional cure may accelerate the development of effective stem cell-based gene therapies for HIV.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146032133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparative Analysis of Cord Blood Mononuclear Cell- and Plasma-Derived Exosomes in Regulating Apoptosis and Proliferation in U937 Cells.","authors":"Bahareh Abbaspanah, Fattah Sotoodehnejadnematalahi, Seyed Hadi Mousavi, Shaban Alizadeh","doi":"10.2174/011574888X426015251128064616","DOIUrl":"https://doi.org/10.2174/011574888X426015251128064616","url":null,"abstract":"<p><strong>Introduction: </strong>Umbilical cord blood-derived exosomes have been considered an excellent resource for regenerative medicine and cancer therapy. These exosomes induce various therapeutic activities through specific cellular responses and molecular signaling mechanisms. In the present study, we investigated the biological effects of exosomes derived from cord blood mononuclear cells (CB-MNCs) and cord blood plasma (CBP) on the U937 cell line, a human acute myeloid leukemia (AML) model.</p><p><strong>Methods: </strong>After cord blood collection, the cord blood units were processed to harvest CBP and CBMNCs. Exosomes were then isolated, purified, and identified by transmission electron microscopy, dynamic light scattering, and Western blotting. Subsequently, U937 cells were cultivated and exposed to the exosomes for 72 hours. Apoptosis and proliferation were assessed by flow cytometry, caspase-3/7 activity assays, and quantitative real-time PCR (qRT-PCR). Moreover, NF-κB and AMPK signaling pathway activity was evaluated by Western blotting.</p><p><strong>Results: </strong>We showed that exosomes from CB-MNCs significantly induced apoptosis, triggered caspase- 3/7 activity, and upregulated the expression of pro-apoptotic genes in U937 cells compared with exosomes from CBP. In addition, CB-MNC-derived exosomes efficiently inhibited NF-κB signaling and activated the AMPK pathway.</p><p><strong>Discussion: </strong>The observed pro-apoptotic effects and pathway modulation indicate that CB-MNCderived exosomes exert stronger anti-leukemic activity than CBP-derived exosomes. These variations could be attributed to the molecular cargo carried by each exosome population and suggest that the cellular source is a significant factor in exosome-based therapeutics.</p><p><strong>Conclusion: </strong>These findings suggest that exosomes derived from CB-MNCs could be a potent antileukemic agent and a promising novel therapeutic approach for AML.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146032113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diverse Programmed Cell Death Patterns and Diagnostic Value in Osteoarthritis Analyzed by Integrative Multiomics Data.","authors":"Mingjie Wei, Xiangwen Shi, Wenbao Tang, Qian Lv, Yipeng Wu, Yongqing Xu","doi":"10.2174/011574888X398652251128074957","DOIUrl":"https://doi.org/10.2174/011574888X398652251128074957","url":null,"abstract":"<p><strong>Introduction: </strong>Chronic pain and high disability rates caused by osteoarthritis (OA) significantly impact quality of life. Programmed cell death (PCD) plays a crucial role in OA pathogenesis; however, a comprehensive analysis of PCD patterns in OA is lacking, limiting understanding of their potential role.</p><p><strong>Methods: </strong>Batch transcriptomic data related to OA were obtained from the GEO database. Differential expression analysis based on 13 PCD patterns was performed to identify differentially expressed genes (DEGs). Unsupervised clustering algorithms were applied to define molecular subtypes associated with OA. The CIBERSORT algorithm was used to analyze the immune microenvironment and evaluate the immunological relevance of each cluster. Hub PCD-related DEGs were identified using a combination of machine learning algorithms, and an OA diagnostic model was constructed based on these hub genes. Single-cell RNA sequencing (scRNA-seq) dataset GSE169454 was used to classify OA chondrocytes into distinct cell clusters. The AddModuleScore function calculated PCD scores, allowing evaluation of hub-gene expression and PCD variation across clusters. Expression levels of ten hub PCD-related DEGs were further validated in OA cells and rat models.</p><p><strong>Results: </strong>Differential expression analysis identified 61 PCD-related DEGs. Unsupervised clustering revealed two molecular subtypes of OA (cluster 1 and cluster 2), with immune-related pathways significantly enriched in cluster 1, including potential NK cell activation. Ten hub PCD-related DEGs were identified using three machine learning algorithms, leading to a highly effective diagnostic model (AUC = 0.993). Five distinct cell types were identified in OA chondrocytes, with higher PCD scores observed in OA samples and the HomC subgroup. Functional analysis indicated significant enrichment of the PI3K-AKT signaling pathway in high BINP3-expressing preHTCs, associated with extracellular matrix composition. mRNA and protein levels of the ten hub DEGs were confirmed in animal models.</p><p><strong>Conclusion: </strong>This multi-omics analysis of 13 PCD patterns provides a preliminary evaluation of the diagnostic and classification value of PCD-related genes in OA, highlighting potential biomarkers for clinical application.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146032165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bibliometric Analysis of the Lung Organoids: Trends, Hotspots, and Future Development.","authors":"Guangping Yang, Hua Zhou, Zhenfang Xiong, Xin Wang, Lanxin Jing, Xinbei Zhang, Zhonghua Fu","doi":"10.2174/011574888X404719251128125512","DOIUrl":"https://doi.org/10.2174/011574888X404719251128125512","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;Organoids are three-dimensional (3D) culture models derived from selforganizing stem cells or progenitor cells, capable of recapitulating the cellular growth microenvironment and interactions within the human body to a certain extent. These models play significant roles in biomedical research, drug screening, and related fields. Research on lung organoids has provided insights into lung development and the cellular and molecular processes underlying diseases, while studies on their transplantation in animals hold promise as novel strategies for post-lung injury therapies. However, no bibliometric studies in the field of lung organoids have been published to date. Using data from the Web of Science Core Collection database, this study employs bibliometric analysis and visualization methods to systematically analyze and summarize the developmental trajectory, research directions, applications, and current research hotspots in lung organoid research. The investigation evaluates existing challenges and proposes future development strategies to facilitate its broader implementation in lung-related investigations.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;This study encompassed literature on pulmonary organoids published between 2010 and 2024, employing Microsoft Excel 2021 for publication trend analysis, while utilizing CiteSpace and VOSviewer software for bibliometric analysis to elucidate interrelationships among authors, institutions, and keywords associated with lung organoids. Citation, co-citation, and burst analysis methods were applied to evaluate research impact and advancements.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;A total of 605 original articles were included in this analysis. The global research landscape shows participation from 50 countries in pulmonary organoid research, with the United States, China, and other nations accounting for the majority of research output. The five most influential authors were identified as Spence, Jason R.; Dye, Briana R.; Morrisey, Edward; Zhou, Su; and Miller, Alyssa J. Through keyword clustering analysis and timeline mapping, this study delineated the principal research domains and the temporal evolution of thematic focuses in lung organoid research. The research hotspots and overall trends across different phases were systematically characterized using literature co-citation burst detection and keyword burst analysis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;Currently, lung organoids are primarily used in the treatment of pulmonary diseases such as idiopathic pulmonary fibrosis (IPF) and are increasingly pivotal in lung cancer research, particularly for elucidating lung cancer pathogenesis and conducting drug screening assays. The research focus on lung organoids has gradually shifted from investigations into culture mechanisms and pulmonary pathophysiological processes to studies involving regenerative medicine and translational medicine. These advancements are expected to emerge as novel ","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146032124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative Approaches to Neural Differentiation: Chondroitin 4-Sulfate and Chondroitinase Induce Differentiation in Human-induced Pluripotent Stem Cells.","authors":"Mohsen Ghiasi, Mohammad-Taher Moradi, Raheleh Halabian, Marzieh Ghollasi, Abdolreza Dayani","doi":"10.2174/011574888X365326250610113501","DOIUrl":"10.2174/011574888X365326250610113501","url":null,"abstract":"<p><strong>Background: </strong>Stem cells have recently gained prominence in regenerative medicine, particularly in the treatment of neurological disorders. As a result, Human-induced Pluripotent Stem Cells (hiPSCs) have become a significant focus.</p><p><strong>Objective: </strong>This study aimed to differentiate hiPSCs into neural lineages under in vitro conditions using forskolin and retinoic acid in an induction medium combined with chondroitin 4-sulfate and chondroitinase.</p><p><strong>Methods: </strong>Optimal component concentrations were determined using the MTT assay and acridine orange/ethidium bromide (AO/EB) staining. Subsequently, neural-specific genes (NSE, MAP-2, β-tubulin III, Oligo-2, and GFAP) and proteins (gamma enolase, MAP-2, and β-tubulin III) were assessed using Real-time PCR analysis and immunofluorescence staining to provide a comprehensive evaluation of differentiated cells.</p><p><strong>Results: </strong>Our study demonstrated a significant enhancement in neural-specific gene and protein markers during the 7th and 14th days of differentiation in the presence of combined chondroitin 4-sulfate and chondroitinase, demonstrating a higher efficacy compared with the application of isolated enzymes or substrates.</p><p><strong>Conclusion: </strong>These findings emphasize the potential importance of chondroitin 4-sulfate and chondroitinase as important factors in promoting the neural differentiation of hiPSCs. It seems that chondroitin 4-sulfate may activate cellular signaling pathways that are effective in inducing neural differentiation. Our findings in this research provide new opportunities to advance regenerative therapies for neurological disorders.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":"66-75"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144478303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Potential of PSC-derived Cell Transplantation in Parkinson's Disease: A Systematic Review and Meta-analysis of Preclinical Studies.","authors":"Aliasghar Karimi, Zahra Shiri, Reza Tabrizi, Somayeh Naderi, Sarvenaz Salahi, Mitra Elmi, Hossein Baharvand","doi":"10.2174/011574888X374086250602050549","DOIUrl":"10.2174/011574888X374086250602050549","url":null,"abstract":"<p><strong>Background: </strong>Parkinson's disease (PD) is a chronic progressive neurodegenerative disease with debilitating clinical presentations. Common therapeutic approaches for symptomatic improvement are often effective for a temporary period of time, after which patients often experience progressive disabilities. Cell replacement therapy is a potential therapeutic method that aims to replace depleted mesencephalic dopaminergic (DA) neurons, which may control symptoms and halt disease progression. Preclinical studies have investigated the efficacy of these PSC-derived DA cells in animal models of PD.</p><p><strong>Methods: </strong>In this study, we comprehensively examine preclinical data on the therapeutic effect of primate PSC-derived DA progenitors on motor deficits in animal models of PD as a precursor for conducting human clinical trials. Relevant articles published before August 14th, 2023, were obtained from PubMed, Scopus, and Web of Science.</p><p><strong>Results: </strong>Through several rounds of screening, 46 studies that met our inclusion criteria were included in this study. The quality of each study was assessed using CAMARADES and SYRCLE approaches. Although no included studies were judged to have an overall high risk of bias, several studies exhibited domain-specific methodological limitations. The analyzed studies demonstrate that cell therapy significantly improves motor dysfunction in rodent and non-human primate models of PD.</p><p><strong>Conclusion: </strong>This systematic review and meta-analysis demonstrate that PSC-based cell therapy significantly improves motor dysfunction in rodent and NHP models of PD and could be a promising approach for halting disease progression, improving behavioral manifestations of the disease, and increasing the overall quality of life in PD.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":"200-215"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144287574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chrysin and Bone Marrow-derived Mesenchymal Stem Cells Restrain Complete Freund's Adjuvant-induced Arthritis in Wistar Rats via Subsiding Inflammation and Oxidative Stress.","authors":"Nermin A Mohamed, Khalid M Mazher, Hesham M Sayed, Mohamed A Abdelaziz, Mohamed A Samhan, Fatma El-Zahraa S Abdel Rahman, Emad A Mahdi, Fatma Mohamed Halfaya, Osama M Ahmed","doi":"10.2174/011574888X367150250701071144","DOIUrl":"10.2174/011574888X367150250701071144","url":null,"abstract":"<p><strong>Introduction: </strong>Rheumatoid arthritis (RA) is an inflammatory disease that causes significant disability and persistent inflammation. Currently, there are no appropriate treatments for RA other than systemic immunosuppressants, which have a variety of undesirable effects after long-term use. Thus, this study aims to determine the anti-arthritis effect of chrysin (5,7-dihydroxyflavone) and/or bone marrow-derived mesenchymal stem cells (BM-MSCs), separately and combined, on CFA (complete Freund's adjuvant)-induced arthritis in rats as an animal model of RA.</p><p><strong>Methods: </strong>Male Wistar rats were subcutaneously injected with 100 μL of CFA/rat/day in the paw of the right hind limb for two consecutive days to induce RA. Arthritic rats received chrysin in an oral dose of 100 mg/kg bw each day, BM-MSCs at 1 × 10⁶ cells/rat once per week in DMEM (Dulbecco's modified Eagle's medium) into the lateral tail vein, and a combination for 21 days.</p><p><strong>Results: </strong>The oral administration of chrysin and intravenous injection of BM-MSCs significantly reduced the increased anteroposterior thickness, volume, and circumference of the right hind paw, as well as serum levels of RF, IL-1β, TNF-α, and IL-17, as well as serum MDA level, besides augmenting serum levels of GPx, GST, GSH, and SOD. The arthritic rats treated with chrysin and/or BMMSCs exhibited a significant improvement in the elevated expression levels of IκBα, NF-κB p50, and NF-κB p65 proteins in ankle joint articular tissue. Similarly, the histopathological score and histological sections provided additional evidence of the improvement in arthritic lesions.</p><p><strong>Discussion: </strong>The treatment with chrysin and BM-MSCs has potential anti-arthritic effects, which may be attributed to their abilities to suppress the inflammation and oxidative stress and enhance the antioxidant defense system. The combinatory effect of chrysin and BM-MSCs was found to be the most effective. However, further clinical studies are required to assess their safety and efficacy in patients with arthritis.</p><p><strong>Conclusion: </strong>Due to their strong antioxidant and anti-inflammatory properties, the combined administration of chrysin and BM-MSCs was found to be more effective in treating arthritis than either treatment alone in Wistar rats.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":"216-232"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144651642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EGFL6 Promotes Angiogenesis and Odontogenesis in Pulp Regeneration <i>via</i> MAPK Signaling Pathways.","authors":"Xiaocui Lu, Yuan Liu, Lin Dai, Bo Cheng, Yanyun Li","doi":"10.2174/011574888X362657250528171158","DOIUrl":"10.2174/011574888X362657250528171158","url":null,"abstract":"<p><strong>Objectives: </strong>Epidermal growth factor-like domain-containing protein 6 (EGFL6) is a member of the epidermal growth factor superfamily. It has been reported that it can enhance the osteogenic differentiation potential of stem cells and stimulate angiogenesis. However, its effects on the regulation of odontogenic differentiation of dental pulp stem cells (DPSCs) have not been studied. Therefore, we aimed to investigate the role of EGFL6 in pulp regeneration and its underlying mechanism.</p><p><strong>Methods: </strong>The cytotoxicity and migration-inductive ability of EGFL6 were evaluated using cell counting kit-8 assay and transwell assay, respectively. A tube formation assay was performed to assess the angiogenic effect of EGFL6. The alkaline phosphatase (ALP) and alizarin red S staining were conducted for mineralization evaluation. The odontoblastic-related and angiogenesis-related markers were measured by quantitative real-time polymerase chain reaction and Western blot analysis. Western blot was also conducted to further examine the levels of key factors involved in MAPK signaling pathways.</p><p><strong>Results: </strong>EGFL6 displayed no cytotoxicity and was capable of promoting cell migration and angiogenesis. Besides, EGFL6 enhanced the mineralization process and up-regulated the expression levels of odontoblastic-related markers (DSPP, DMP1, and BSP) after 5, 7, and 10 days. The expression levels of odontoblastic-related and angiogenesis-related proteins (DSPP, DMP1, VEGF, and ALP) could all be up-regulated by EGFL6. There was also an increase in the phosphorylation levels of ERK1/2 and P38.</p><p><strong>Conclusion: </strong>EGFL6 can promote the migration, angiogenesis, and odontogenesis differentiation of DPSCs via the activation of MAPK signaling pathways.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":"76-90"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144228044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into Endometriosis Organoids Based on Uterine Tissue Engineering: A Mini-review.","authors":"Zeinab Nurian, Alireza Ebrahimzadeh, Fatemeh Alipour, Hengameh Dortaj","doi":"10.2174/011574888X360426250529055140","DOIUrl":"10.2174/011574888X360426250529055140","url":null,"abstract":"<p><p>Endometriosis is a chronic condition where tissue similar to the endometrium grows outside the uterus, affecting 5-10% of women and causing pelvic pain, painful periods, and infertility. Diseases of the endometrium, the lining of the uterus, can lead to a variety of reproductive health issues, including infertility, irregular bleeding, and endometrial cancer. Researchers have developed advanced in vitro systems using uterine organoids and decellularized tissue scaffolds to understand and model these diseases. The main limitations of traditional 2D monolayer cultures include reduced biological activity, reduced hormone responsiveness, and lack of interaction with ECM. Researchers have investigated 3D culture approaches to address these shortcomings, such as scaffold-free organoids and decellularized tissue scaffolds. Organoid systems can better recapitulate the cellular heterogeneity and physiological functions of the native endometrium. Decellularization protocols have been optimized to generate intact uterine scaffolds that preserve the structural and compositional features of the ECM. Implantation of these bioscaffolds into animal models demonstrated their biocompatibility and regenerative potential. Further refinements of organoid and scaffold technologies, including chemically defined matrices and organ-on-a-chip platforms, will improve our ability to model the uterus. Integration of these advanced in vitro models with patient-derived cells will enable personalized disease modeling and the development of targeted therapies. The combination of organoids, decellularized scaffolds, and microfluidic technologies holds great potential for exploring reproductive biology, drug screening, and developing regenerative therapies for uterine diseases and infertility.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":"1-10"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144259696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting FBN1 and BMP-8: The Potential Mechanism of SDF-1 in Acute Spinal Cord Injury Repair.","authors":"Quan Sun, Xufeng Jiang, Youzhi He, Li Yang, Yi Luo","doi":"10.2174/011574888X359624250901071217","DOIUrl":"10.2174/011574888X359624250901071217","url":null,"abstract":"<p><strong>Introduction: </strong>Acute Spinal Cord Injury (SCI) often causes motor and sensory deficits. SDF-1 promotes stem cell survival and proliferation, while FBN1 may impact repair mechanisms. This study investigates how SDF-1 promotes SCI treatment by inducing BMSC maturation through BMP-8-mediated FBN1 inhibition.</p><p><strong>Methods: </strong>Bone marrow mesenchymal stem cells were induced to differentiate with BMP-8 and transfected with related plasmids (oe-NC, oe-SDF-1, oe-FBN1, si-BMP-8). CCK-8 and alizarin red staining were used to assess cell growth and differentiation. Western blotting was used to detect the levels of SDF-1, FBN1, and BMP-8. In a rat SCI model, cells with plasmids were injected, and motor recovery was assessed using BBB scoring. Immunofluorescence assay detected SDF-1 expression, while Western blotting was used to detect SDF-1, FBN1, and BMP-8.</p><p><strong>Results: </strong>In cell experiments, BMP-8 induced successful differentiation of BMSCs. After overexpression of SDF-1, the proliferation and differentiation of BMSCs were increased. In animal experiments, the BBB score increased after overexpression of SDF-1.</p><p><strong>Discussion: </strong>These findings suggest a potential therapeutic mechanism in which SDF-1 promotes spinal cord repair by modulating the BMP-8/FBN1 axis. The suppression of FBN1 appears to be a key step in enhancing BMSC function. Targeting this pathway could offer new strategies for regenerative treatment following SCI.</p><p><strong>Conclusion: </strong>In acute spinal cord injury, SDF-1 enhances the differentiation of bone marrow mesenchymal stem cells induced by BMP-8 through the suppression of FBN1.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":"409-417"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145294795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}