缺氧条件下 miR-98/IL-6/STAT3 对体外心脏干细胞自噬和凋亡的影响

Xueyuan Li, Yang Zhang, Guangwei Zhang
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摘要

背景:心血管疾病带来的沉重负担需要创新的治疗策略来应对心肌细胞的丧失。心脏干细胞(CSCs)是心肌中可再生的细胞,具有分化和内分泌功能。然而,在严重缺氧或炎症的情况下,它们的功能会受到明显抑制。缺氧影响 CSCs 的机制尚不清楚。白细胞介素-6(IL-6)在缺氧和炎症微环境中似乎都很活跃。本研究旨在探讨在严重缺氧条件下,IL-6是否与CSC凋亡和自噬有关:方法:本研究采用交替消化法提取大鼠 CSCs。方法:本研究采用交替消化法提取大鼠CSCs,用双荧光素酶法检测miR-98与IL-6 mRNA的相互作用,并用qPCR证实miR-98对IL-6表达的影响。流式细胞术检测了IL-6对CSC凋亡的影响,透射电子显微镜检测了IL-6对CSC自噬的影响。采用Western印迹法检测IL-6对细胞凋亡和自噬相关蛋白表达的影响。统计分析采用方差分析和 Dunnett T3 检验。当 P < 0.05 时,差异具有显著性:结果:在严重缺氧条件下,IL-6能显著增加CSC的凋亡并降低p-STAT3的表达。在严重缺氧条件下,抑制STAT3信号通路后,CSC凋亡明显增加。在严重缺氧条件下,IL-6还能明显抑制CSCs的自噬,阻断其自噬流。结论:研究发现,miR-98/IL-6/STAT3参与了严重缺氧条件下CSCs凋亡和自噬的调控,CSCs的凋亡和自噬之间可能存在相互联系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of miR-98/IL-6/STAT3 on Autophagy and Apoptosis of Cardiac Stem Cells Under Hypoxic Conditions In vitro.

Background: The heavy burden of cardiovascular diseases demands innovative therapeutic strategies dealing with cardiomyocyte loss. Cardiac Stem Cells (CSCs) are renewable cells in the myocardium with differentiation and endocrine functions. However, their functions are significantly inhibited in conditions of severe hypoxia or inflammation. The mechanism of hypoxia affecting CSCs is not clear. Interleukin-6 (IL-6) appears active in both hypoxic and inflammatory microenvironments. The aim of this study was to explore whether IL-6 is related to CSC apoptosis and autophagy under severe hypoxia.

Methods: In this study, rat CSCs were extracted by alternate digestion. The interaction of miR-98 and IL-6 mRNA was detected by the dual luciferase method, and qPCR was applied to confirm the effect of miR-98 on IL-6 expression. The effect of IL-6 on CSC apoptosis was measured by flow cytometry and the effect of IL-6 on CSC autophagy by transmission electron microscopy. The western blot method was applied to detect the effect of IL-6 on the expressions of proteins related to apoptosis and autophagy. ANOVA and Dunnett T3's test were employed in the statistical analysis. When p < 0.05, the difference was significant.

Results: Under severe hypoxia conditions, IL-6 increased CSC apoptosis and decreased p-STAT3 expression significantly. CSC apoptosis increased significantly after inhibition of the STAT3 signaling pathway under severe hypoxia. IL-6 could also significantly inhibit CSCs' autophagy and block their autophagy flow under severe hypoxic conditions. Meanwhile, it was confirmed that miR-98 had a binding site on IL-6 mRNA and miR-98 significantly inhibited IL-6 mRNA expression in CSCs under severe hypoxic conditions.

Conclusion: miR-98/IL-6/STAT3 has been found to be involved in the regulation of CSCs' apoptosis and autophagy under severe hypoxic conditions and there might be a mutual linkage between CSCs' apoptosis and their autophagy.

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