Current stem cell research & therapy最新文献

{"title":"Identification and Functional Characterization of CDK1, CCNB1, CCNB2, RRM2, and GTSE1 Associated with Replicative Senescence of Human Umbilical Cord-Derived Mesenchymal Stem Cells.","authors":"Wenxia Chen, Liming Ouyang, Lixin Zhang, Xiaopeng Jia","doi":"10.2174/011574888X508076260925100358","DOIUrl":"https://doi.org/10.2174/011574888X508076260925100358","url":null,"abstract":"<p><strong>Introduction: </strong>Replicative senescence limits the expansion and therapeutic potential of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs). This study aimed to identify passage-associated candidate genes and assess their relationships with senescence-associated phenotypes.</p><p><strong>Methods: </strong>The RNA-seq dataset GSE233634 was reanalyzed using 12 planar-culture samples from Donor 1 at p3, p6, p9, and p12. Candidate expression was examined by qRT-PCR in Donors 2-4, and siRNA experiments were performed in Donor 4-derived p12 cells. Senescence-associated features were evaluated using morphology, SA-β-galactosidase staining, CCK-8-derived metabolic activity, cell-cycle distribution, and reactive oxygen species (ROS).</p><p><strong>Results: </strong>Compared with p6 cells, p12 cells showed enlarged and flattened morphology, a higher SA- β-galactosidase-positive area fraction, lower CCK-8-derived metabolic activity, reduced S-phase representation, G0/G1 accumulation, and increased ROS. Transcriptomic screening identified 405 genes shared among three p12 comparisons, of which 205 progressively increased with passage. CDK1, CCNB1, CCNB2, RRM2, and GTSE1 were selected as senescence-associated candidates and showed consistent passage-associated mRNA increases in Donors 2-4. CDK1, CCNB1, CCNB2, and RRM2 were also supported at the protein level, whereas GTSE1 was supported at the mRNA level only. Candidate-gene knockdown was associated with differential changes in selected senescenceassociated readouts, with CCNB1 knockdown showing the broadest pattern.</p><p><strong>Discussion: </strong>These findings link the five candidates to passage-associated phenotypes but do not establish direct regulatory causality or reversal of senescence.</p><p><strong>Conclusion: </strong>The five genes represent candidates for further mechanistic investigation in hUC-MSC replicative senescence.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149139968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wnt5a-Modified Mesenchymal Stem Cells Promote the Repair of Cartilage Defects.","authors":"Zhifang Wu, Fosheng Zheng, Yiwen Luo, Liangliang Xu, Zhao Gao","doi":"10.2174/011574888X481777260916113837","DOIUrl":"https://doi.org/10.2174/011574888X481777260916113837","url":null,"abstract":"<p><strong>Introduction/objective: </strong>Cartilage tissue exhibits limited ability to self-renew after injury, which leads to mobility impairment and even disability. To investigate the regulatory effect of Wnt5a on chondrogenesis of Mesenchymal Stem Cells (MSCs), and evaluate the application effect of Wnt5a-overexpressing MSCs in cartilage defect repair.</p><p><strong>Methods: </strong>Transwell assay and scratch assay were used to check cell migration capacity, and chondrogenic induction assay was employed to verify the level of chondrogenic differentiation potential. Western Blot was performed to detect the relative levels of protein. An animal model of cartilage defect was established, and Wnt5a-overexpressing MSCs were implanted to assess the repair effect.</p><p><strong>Results: </strong>Compared with the empty vector control, Wnt5a overexpression significantly enhanced the proliferation activity and migration ability of MSCs, and enhanced the chondrogenic potential of MSCs. Moreover, Wnt5a overexpression could significantly up-regulate the protein expression levels of p-Akt and p-JNK in MSCs, thereby activating the PI3K/AKT and JNK signaling pathways. Finally, the in vivo experiments confirmed that MSCs overexpressing Wnt5a could effectively promote the repair of cartilage defects.</p><p><strong>Discussion: </strong>Modifying MSCs with Wnt5a to enhance their biological functions can be used to improve cartilage defect repair.</p><p><strong>Conclusion: </strong>Wnt5a controls MSC proliferation, migration, and chondrogenesis, likely through the PI3K/AKT and JNK signaling pathways. Wnt5a-overexpressing MSCs have the effect of promoting cartilage defect repair.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149120712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell Therapy for Heart Failure and Myocardial Infarction: Evidence Evolution and Translational Challenges Over Two Decades.","authors":"Weizhong Zhuang, Weimin Chen, Song Yang, Luoming Hu, Qiang Gao, Jianzheng Cen","doi":"10.2174/011574888X500658260921112146","DOIUrl":"https://doi.org/10.2174/011574888X500658260921112146","url":null,"abstract":"<p><strong>Introduction: </strong>Cell-based myocardial repair has progressed from direct cell replacement toward paracrine, engineered tissue, and cell-free strategies, but the relationship between preclinical innovation and clinical translation remains unclear. We mapped the knowledge structure and translational trajectory of this field.</p><p><strong>Methods: </strong>Original research articles on cell therapy for myocardial infarction or heart failure published from 2004 through 2024 were retrieved from the Web of Science Core Collection. The 1,505 included records were classified as in vitro, animal, or clinical studies; animal and clinical subsets were then compared. Publication, collaboration, co-citation, citation-burst, keyword, and study-type- stratified analyses were performed using VOSviewer, CiteSpace, and the bibliometrix package in R.</p><p><strong>Results: </strong>Annual output increased from 34 articles in 2004 to a peak of 104 in 2012 and declined to 49 in 2024. The corpus comprised 1,190 animal and 281 clinical studies; clinical output peaked at 26 articles in 2011 and fell to 4 in 2024. The United States and China contributed the most publications, with 82 China-United States collaborations. Co-citation and burst analyses identified early bone marrow- derived and progenitor-cell trials as major intellectual landmarks. Thematic emphasis subsequently shifted toward mesenchymal stromal cells, induced pluripotent stem cell-derived cardiomyocytes, tissue engineering, extracellular vesicles, inflammation, maturation, and electrical integration. Stratified keyword analysis showed that animal studies emphasized mesenchymal stromal cells, cardiomyocytes, and extracellular vesicles. In contrast, clinical studies more often emphasized progenitor cells, bone marrow mononuclear cells, endothelial progenitor cells, and intracoronary delivery.</p><p><strong>Discussion: </strong>These findings indicate that the field has gradually shifted away from attempts at direct myocardial replacement and toward strategies that emphasize mechanistic understanding, delivery optimization, and paracrine effects. Persistent translational bottlenecks appear to have redirected research priorities across successive phases of cardiac cell therapy development.</p><p><strong>Conclusion: </strong>Preclinical diversification has continued despite contraction of clinical activity, indicating that innovation has outpaced clinical translation. This bibliometric analysis identifies where the animal-clinical gap is concentrated and provides an evidence map for prioritizing reproducible, mechanism-informed, and clinically feasible strategies; it does not establish therapeutic efficacy.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149120616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stem Cell-Derived Exosomes Therapy for Spinal Cord Injury: Mechanisms and Prospects.","authors":"Jie Yan, Cancan Wang, Jinghan Wang, Xinyu Yuan, Fujun Li, Yanqiu You, Dianliang Lin, Zhongquan Qi","doi":"10.2174/011574888X454755260727230528","DOIUrl":"https://doi.org/10.2174/011574888X454755260727230528","url":null,"abstract":"<p><p>Spinal cord injury (SCI) is a severe neurological condition often leading to enduring motor, sensory, and autonomic dysfunction. Its pathophysiology involves primary mechanical damage followed by secondary injury processes including axonal disruption, demyelination, excitotoxicity, neuroinflammation, vascular dysfunction, and cell death. Effective treatments that restore neurological function remain scarce. Exosomes (Exos) are nanoscale extracellular vesicles that mediate intercellular communication. Evidence indicates that Exos derived from mesenchymal stem cells (MSCs), neural stem cells (NSCs), and other progenitor populations produce therapeutic effects in SCI models. These effects are mediated through modulation of neuroinflammation, inhibition of apoptosis, preservation of vascular integrity, facilitation of local axonal sprouting and adaptive plasticity within spared neural tissue, and promotion of remyelination. Compared with cell-based therapies, Exos offer lower immunogenicity, reduced tumorigenic potential, and improved stability. Nevertheless, clinical translation faces obstacles including Exos heterogeneity, low yield, limited targeting specificity, and lack of standardized isolation and storage protocols. Emerging approaches such as Exos engineering, artificial Exos, and gene delivery systems are under investigation. This review summarizes SCI pathology and evaluates the therapeutic potential, limitations, and future directions of Exos-based interventions. Importantly, existing data suggest that Exos predominantly influence the post-injury microenvironment and enhance adaptive plasticity rather than promoting true long-distance axonal regeneration. This distinction requires rigorous validation in future studies.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149120666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Effects of Herbal Bioactive Compounds and Growth Factors on Osteogenic Differentiation of Dental Derived Mesenchymal Stem Cells.","authors":"Khadijeh Khezri, Ashrafalsadat Hatamian-Zarmi, Bahman Ebrahimi Hoseinzadeh, Majid Saeedi, Nastaran Hashemzadeh, Solmaz Maleki Dizaj","doi":"10.2174/011574888X480815260912091035","DOIUrl":"https://doi.org/10.2174/011574888X480815260912091035","url":null,"abstract":"<p><p>Repairing large bone defects is still not an easy task and is considered an important challenge in regenerative medicine and maxillofacial surgery. The methods that currently exist each have their own limitations. Bone grafting from the patient's own body is known as the best method, but it is not always possible. This is because the donor site may get damaged, the amount of bone that can be taken is limited, and the surgery itself is complicated. In recent years, stem cells that are isolated from dental tissues have received a lot of attention. These cells proliferate quickly, have the ability to turn into different types of cells, and can even modulate immune responses. Because of this, they are considered a good option for bone regeneration. Alongside the use of stem cells, plant‑derived bioactive compounds have also become important. These compounds are natural, biocompatible, and can influence major cellular pathways that play roles in bone formation. This article discusses the simultaneous use of plant compounds and growth factors to enhance the bone‑forming ability of dental stem cells. Compounds such as flavonoids, polyphenols, and terpenoids can activate key signaling pathways like Bone Morphogenetic Protein (BMP), Wingless-related integration site /Beta-catenin (Wnt/β‑catenin), Mitogen-Activated Protein Kinase (MAPK), and Phosphoinositide 3-kinase/Protein Kinase B (Akt) (PI3K/Akt). This activation improves the bone‑formation process and also reduces inflammation and oxidative stress. When these plant compounds are used together with growth factors, their effect becomes stronger. Growth factors such as BMP, IGF, and FGF play important roles in regulating cell growth and differentiation, and combining them with plant compounds leads to better cellular responses. In this way, there is no longer a need to use high doses of growth factors. Generally, the simultaneous use of plant compounds and growth factors can be a promising and practical method for the future of bone tissue engineering and regenerative medicine.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149120691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Current Landscape and Prospects of iPSCs in BCMA-targeted Therapy for Multiple Myeloma.","authors":"Mengyang Wu, Xiafei Pan, Yuxuan Zhong, Minxia Ke, Yuehong Wu","doi":"10.2174/011574888X492765260910100313","DOIUrl":"https://doi.org/10.2174/011574888X492765260910100313","url":null,"abstract":"<p><p>Multiple Myeloma (MM) is a difficult-to-treat plasma-cell malignancy with heterogeneous pathogenesis and a poor prognosis. Given the limitations of existing treatments, developing approaches that improve efficacy and safety and ultimately achieve long-term remission or even a cure for MM is crucial. The high expression of B-Cell Maturation Antigen (BCMA) on malignant Plasma Cells (PCs) makes BCMA an important therapeutic target in MM. BCMA-targeted therapies have produced major advances in the treatment of MM. Here, we summarize conventional regimens and their limitations and examine how restricted cell sources, treatment resistance, and toxicity affect therapeutic outcomes. To address these limitations, MM treatment has entered an era of targeted therapy and immunotherapy; even though CAR-T therapy can induce deep and durable remissions, there are still unmet clinical needs. Induced pluripotent stem cell (iPSC)-based therapies are expected to provide more effective treatment options for MM patients through strategies such as large-scale production of off-the-shelf engineered immune cells and combined therapy.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149042679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Inflammatory Effects of Adipose-Derived Mesenchymal Stem Cells and Their Exosomes in the Cortex and Striatum in a Nonalcoholic Steatohepatitis Model.","authors":"Nahid Jashirenezhad, Zahra Moayedfard, Maryam Hosseini, Zahra Jafarinezhad, Fatemeh Derisfard, Farhad Koohpeyma, Negar Azarpira, Hadi Aligholi","doi":"10.2174/011574888X431196260908071718","DOIUrl":"https://doi.org/10.2174/011574888X431196260908071718","url":null,"abstract":"<p><strong>Introduction: </strong>Brain injury associated with Non-Alcoholic Fatty Liver Disease (NAFLD) has emerged as an important health concern, yet effective therapeutic strategies remain limited. Recently, Adipose-Derived Mesenchymal Stem Cells (AD-MSCs) and their Exosomes (AD-MSCExosomes) have attracted attention as promising therapeutic approaches. This study investigated the protective effects of AD-MSCs and their exosomes on the cortex and striatum in a mouse model of Non-Alcoholic Steatohepatitis (NASH).</p><p><strong>Methods: </strong>Male C57BL/6 mice were fed a High-Fat Diet (HFD) for eight weeks to induce NASH. During the 6th, 7th, and 8th weeks, mice received intravenous injections of either 1×10⁶ AD-MSCs or 100 μg of exosomes. The percentage of degenerating cells in the cortex and striatum was assessed, and the gene expression levels of TNF-α, IL-1β, IL-10, and BDNF were evaluated in both brain regions.</p><p><strong>Results: </strong>NAFLD significantly increased the percentage of degenerating cells in the cortex and striatum. Treatment with AD-MSCs and their exosomes markedly reduced cellular degeneration in both regions. In addition, NAFLD induced overexpression of BDNF and IL-1β in the cortex, as well as BDNF and TNF-α in the striatum, while IL-10 expression in the striatum was decreased. Most therapeutic interventions effectively reversed these alterations.</p><p><strong>Discussion: </strong>The findings indicate that AD-MSCs and their exosomes may exert neuroprotective effects against NAFLD-associated brain injury, potentially through modulation of inflammatory pathways and reduction of neuronal degeneration in the cortex and striatum.</p><p><strong>Conclusion: </strong>AD-MSCs and their exosomes attenuated brain tissue degeneration and modulated inflammatory cytokine expression in the cortex and striatum, suggesting their potential as therapeutic candidates for NAFLD-related neurological complications.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149019158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms and Therapeutic Advances of M2 Macrophages and Mesenchymal Stem Cell-derived Exosomes in Diabetic Wound Healing.","authors":"Tengxiao Ma, Haoxinai Wang, Lei Li","doi":"10.2174/011574888X496389260907075713","DOIUrl":"https://doi.org/10.2174/011574888X496389260907075713","url":null,"abstract":"<p><p>Diabetic wound healing remains one of the most significant clinical challenges for surgeons and endocrinologists, given that impaired immune regulation and blocked local tissue repair universally occur in diabetic wound microenvironments. M2 macrophages and exosomes derived from Mesenchymal Stem Cells (MSC-Exos) have emerged as promising therapeutic candidates, owing to their core functions in immune modulation and tissue regeneration. In this review, we systematically elaborate the immunomodulatory properties and polarization mechanisms of M2 macrophages in diabetic wounds, with an emphasis on their capacity to relieve chronic inflammation and facilitate tissue repair. We further summarize the biological characteristics of MSC-Exos and clarify the molecular mechanisms by which these vesicles accelerate wound healing and Extracellular Matrix (ECM) remodeling. More importantly, we focus on the bidirectional synergistic crosstalk between M2 macrophages and MSC-Exos in diabetic wound microenvironments, and highlight their great therapeutic potential for refractory diabetic wounds. Combined with the latest preclinical progress and translational research, we analyze the practical application value of M2 macrophages and MSCExos in diabetic wound management. This review integrates current basic research and preclinical data, aims to lay a solid theoretical basis for targeted intervention in diabetic wounds, and proposes novel combined therapeutic strategies to improve wound healing efficacy.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149013445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Dental Pulp Stem Cells (DPSCs) in Periodontally Accelerated Osteogenic Orthodontics (PAOO) for Enhancing Alveolar Bone Thickness and Reducing Orthodontic Treatment Duration-A Pilot Clinical Study.","authors":"Shantha Sundari K K, Shyamala Chandrasekar, Rajagopalan Vijayaraghavan, Gurumoorthy Kaarthikeyan, Poornima Ravi, Rajappa Senthilkumar, Mathaiyan Rajmohan, Ramalingam Karthick, Senthilkumar Preethy, Samuel Jk Abraham","doi":"10.2174/011574888X485355260901075010","DOIUrl":"https://doi.org/10.2174/011574888X485355260901075010","url":null,"abstract":"<p><strong>Introduction: </strong>Adult orthodontic patients often require prolonged treatment and heightened periodontal risks, especially in bimaxillary protrusion with thin labial bone. Periodontally Accelerated Osteogenic Orthodontics (PAOO) has been shown to shorten treatment duration and enhance periodontal safety. This quasi-experimental pilot study evaluated the feasibility of PAOO using Freeze- Dried Demineralized Bone Allograft (FDDBA) with or without Dental Pulp Stem Cells (DPSCs) embedded in Festigel.</p><p><strong>Methods: </strong>Thirty adults were assigned to Group I: conventional treatment (control), Group II: PAOO with FDDBA, or Group III: PAOO with FDDBA + DPSCs in a Festigel scaffold. This was a prospective, non-randomized, patient-preference quasi-experimental pilot study. DPSCs were isolated, cultured, and delivered in a Festigel scaffold during corticotomy. The primary outcome measure was orthodontic treatment duration. Secondary outcomes included alveolar bone thickness and root resorption assessed using CBCT-based qualitative grading.</p><p><strong>Results: </strong>Twenty-two participants completed the study: Group I, control (n=9); Group II, PAOO with FDDBA (n=7); and Group III, PAOO with FDDBA + DPSCs (n=6). Both PAOO groups showed significantly shorter treatment duration, approximately 13.5 months, compared with the control group, 24.8 months. Qualitative CBCT-based grading showed the greatest improvement in bone thickness in the PAOO + FDDBA + DPSCs group, followed by the PAOO + FDDBA group. However, the difference between these two groups was not statistically significant. Periodontal health remained excellent throughout the study in all groups. No serious adverse events were observed during the available follow-up period.</p><p><strong>Discussion: </strong>PAOO with grafting, with or without DPSCs, reduced orthodontic treatment time and helped maintain alveolar bone thickness compared with conventional orthodontics. Although the PAOO + FDDBA + DPSCs group showed a favourable trend in bone thickness, the added clinical benefit of DPSCs over graft alone was not statistically significant.</p><p><strong>Conclusion: </strong>This first-of-its-kind pilot clinical study using DPSCs in PAOO demonstrated that both PAOO approaches, with and without DPSCs, reduced orthodontic treatment time and maintained alveolar bone thickness compared with conventional orthodontics. However, the improvement with DPSCs was modest and statistically non-significant. Future large-scale, longer-duration clinical trials are needed to validate the DPSC-based PAOO approach for routine clinical application.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148972709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LncRNA HIF1A-AS3 Promotes Glycolysis and Regulates Metabolic Reprogramming in Human Mesenchymal Stem Cells.","authors":"Jiajin Xu, Xiaotong Dong, Yang Zhao, Hailong Gu, Ruoyu Yang, Xu Zhang, Xiaoxin Zhang, Min Fu, Jiahui Zhang, Runbi Ji","doi":"10.2174/011574888X492037260825095121","DOIUrl":"https://doi.org/10.2174/011574888X492037260825095121","url":null,"abstract":"<p><strong>Introduction: </strong>Mesenchymal stem cells (MSCs) play a significant role in shaping and regulating microenvironments. The factors influencing the glycolytic metabolic reprogramming of MSCs remain unknown.</p><p><strong>Methods: </strong>MSCs were isolated from human umbilical cords and identified using flow cytometry. Glycolysis-related gene expression, glucose consumption, and ATP and lactate production were measured in MSCs with HIF1A-AS3 overexpression or knockdown. The roles of HIF1A-AS3 in the glycolytic pathway and its downstream network were revealed by high-throughput sequencing.</p><p><strong>Results: </strong>HIF1A-AS3 enhanced the glycolytic level of MSCs by increasing glucose consumption, lactate production, ATP levels, and expression of key glycolytic enzymes (GLUT1, HK2, and PKM2). The downstream signaling pathway of HIF1A-AS3 might be associated with glycosylationrelated diseases.</p><p><strong>Discussion: </strong>It was demonstrated that HIF1A-AS3, a hypoxia-induced long non‑coding RNA, acted as a critical regulator of glycolytic reprogramming in MSCs. Additionally, HIF1A-AS3 upregulated a distinct subset of interferon‑stimulated genes, suggesting a potential link between metabolic reprogramming and immunomodulation in MSCs. Further mechanistic studies are warranted to elucidate how HIF1A-AS3 regulates these processes.</p><p><strong>Conclusion: </strong>The study reveals that HIF1A-AS3 is a regulator of the metabolic reprogramming of glycolysis in MSCs. These findings provide new insights into understanding the metabolic regulatory mechanism of MSCs.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148908305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}