Current stem cell research & therapy最新文献

{"title":"Human Wharton's Jelly Mesenchymal Stem Cells and their Extracellular Vesicles in the Management of Bleomycin-induced Lung Injury in Model Animals: A Comparative Preclinical Study Focused on Histomorphometric Analysis.","authors":"Anand Krishnan, V S Harikrishnan, A Sabareeswaran, Naresh Kasoju","doi":"10.2174/011574888X366742250417065341","DOIUrl":"https://doi.org/10.2174/011574888X366742250417065341","url":null,"abstract":"<p><strong>Introduction: </strong>Pulmonary fibrosis, a condition characterized by excessive lung tissue scarring, remains a significant therapeutic challenge. Given the potential of human Wharton's jelly- derived mesenchymal stem cells (hWJ-MSCs) and their extracellular vesicles (hWJ-MSC-EVs) as minimally invasive and scalable therapeutic options for pulmonary fibrosis in clinical settings, this study investigates the potential of hWJ-MSCs and hWJ-MSC-EVs in mitigating bleomycin-induced lung injury in C57BL/6J mice.</p><p><strong>Methods: </strong>hWJ-MSCs were cultured and characterized for their ability to differentiate into osteogenic, adipogenic, and chondrogenic lineages. EVs were successfully induced via serum starvation, purified using ultracentrifugation, and characterized for their protein and nucleic acid content, size distribution, and EV markers. A bleomycin-induced pulmonary fibrosis model was established in C57BL/6J mice. Mice were monitored for weight loss, mortality, and lung fibrosis severity following treatment with hWJ-MSCs and hWJ-MSC-EVs. Histological analysis and Ashcroft scoring were used to assess lung fibrosis.</p><p><strong>Results: </strong>Bleomycin administration in mice resulted in significant weight loss, increased mortality, and severe lung fibrosis, as demonstrated by histological analysis and Ashcroft scoring. Treatment with hWJ-MSCs and hWJ-MSC-EVs significantly alleviated these symptoms. Mice receiving these treatments exhibited improved body weight, enhanced survival rates, and reduced lung fibrosis, with notable improvements in alveolar structure and decreased fibrotic tissue deposition.</p><p><strong>Conclusions: </strong>These findings highlight the potential of hWJ-MSCs and hWJ-MSC-EVs as therapeutic agents in treating pulmonary fibrosis by reducing inflammation and promoting lung tissue repair, offering a potential new avenue for regenerative therapy in severe lung diseases. Future research directions involve elucidating the molecular pathways involved in tissue repair, optimizing therapeutic delivery, and conducting comprehensive clinical evaluations.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144059061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous Co-transplantation for Highly Efficient Cell Therapy.","authors":"Ji-Hee Choi, Mingu Ryu, Sung-Hwan Moon","doi":"10.2174/011574888X359983250408105711","DOIUrl":"https://doi.org/10.2174/011574888X359983250408105711","url":null,"abstract":"<p><p>Cell therapy involves transplantation of cells to replace damaged tissues and cells and is used in regenerative medicine. Since its introduction, numerous cell therapy modalities have been developed to treat various diseases, and cell therapy has shifted the paradigm of the treatment of degenerative and refractory diseases. However, it faces limitations in terms of long-term therapeutic effects and efficiency. To overcome these challenges, the concept of co-transplantation, which utilizes two different cell sources, has been proposed. Stem cell-based co-transplantation approaches have been extensively studied both experimentally and clinically for various diseases, including graftversus- host disease (GVHD), infertility, acute liver failure (ALF), and myocardial infarction (MI). These have yielded improved transplantation efficiency and stability compared to single-cell transplantation methods. This review examines the development and effectiveness of co-transplantation through its application in four diseases. Additionally, it discusses the clinical applicability of cotransplantation, explores future research directions, and highlights its potential benefits.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Human Embryonic Stem Cell-derived Neural Stem Cell Senescence Model Triggered By Oxidative Stress","authors":"Hui Pan, Li Bao, Meng Ji, Zhengbing Lyu, Nianmin Qi, Yuehong Wu","doi":"10.2174/011574888X365639250214045110","DOIUrl":"10.2174/011574888X365639250214045110","url":null,"abstract":"<p><strong>Introduction: </strong>Neural stem cells (NSCs) are vulnerable to oxidative stress, which triggers aging and subsequently leads to a reduced regenerative capacity of the central nervous system (CNS). Due to the challenges in acquiring aged human NSCs and the lack of an oxidative stressinduced aging model specifically designed for human NSCs, research related to the aging mechanisms and the screening of anti-aging drugs have been limited. Here, we aimed to establish an oxidative stress-induced senescence model of NSCs by using D-galactose (D-gal).</p><p><strong>Methods: </strong>Human embryonic stem cells (hESC) were differentiated into hESC-NSCs using a type I collagen method. hESC-NSCs were characterized by flow cytometry combined with immunofluorescence. A senescence model of hESC-NSCs was established using D-gal and characterized by CCK-8 assay, neurosphere formation, crystal violet staining, DNA damage assay, SA-β-gal staining and ROS levels measurement. To further explore the profile of gene expression in D-gal-induced hESCNSCs senescence model, transcriptome sequencing was performed and analysed by bioinformatics method, following verified by qPCR.</p><p><strong>Results: </strong>The hESC-derived NSCs senescence model demonstrated reduced proliferation and elevated β-galactosidase activity, accompanied by DNA damage and increased levels of reactive oxygen species. Furthermore, transcriptome analysis unveiled the potential central role of the MAPK signaling pathway in D-gal-induced senescence, which involves the key genes including DDIT3, ATF3, CEBPB, JUN, and CCND1.</p><p><strong>Conclusion: </strong>We presented an oxidative stress-induced senescence model of hESC-NSCs and identified key pathways and genes related to D-gal-induced senescence. Our study might offer an alternative approach for investigating human NSC aging and provide valuable data for understanding the underlining mechanisms of oxidative stress-induced aging.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143470423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organoids for Obesity-related Diseases: Disease Models and Drug Screening.","authors":"Jiaman Xie, Keyi Zhou, Hanyu Zhang, Zhijia Jiang, Jingxian Fang","doi":"10.2174/011574888X357011250406044043","DOIUrl":"https://doi.org/10.2174/011574888X357011250406044043","url":null,"abstract":"<p><strong>Background: </strong>Organoids are three-dimensional structures that faithfully mimic the intricate internal environment of the human body. Compared to conventional models, they demonstrated superior performance. Recently, they have emerged as valuable platforms for modeling obesity- related diseases and advancing therapeutic strategies.</p><p><strong>Objective: </strong>This review not only aimed to simply discuss the limitations of 2D cellular and animal models for obesity-related diseases but also highlighted the importance of developing organoids to better understand the relationship between obesity, lipid metabolism, glucose homeostasis, and chronic inflammation. It also identifies the challenges and potential directions for organoid applications in these diseases.</p><p><strong>Methods: </strong>We searched for keywords related to organoids, obesity, lipid metabolism, glucose homeostasis, chronic inflammation, disease models, and drug screening in scientific research databases.</p><p><strong>Results: </strong>Organoids have emerged as promising tools for investigating the pathophysiology of diseases and developing therapeutic interventions. They have effectively bridged the gap in research on obesity-related diseases between conventional experimental models and the human body. They could offer more efficient and physiologically relevant experimental models while also improving the treatment efficacy for individuals with obesity-related conditions.</p><p><strong>Conclusion: </strong>Organoids are beneficial for investigating obesity-related diseases. However, it is imperative to implement standardised culture procedures to improve reproducibility and broaden their application. Combining medicine and science to create these processes and minimise variation can increase the reliability and consistency of organoid cultures and provide new opportunities for addressing obesity-related diseases.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Stem Cell-derived Extracellular Vesicles in the Treatment of Alzheimer's Disease Model Mice: A Systematic Review and Meta-analysis.","authors":"Qinpin Zheng, Sensen Wang, Tian Wang, Guirong Zhang","doi":"10.2174/011574888X352270250407170235","DOIUrl":"https://doi.org/10.2174/011574888X352270250407170235","url":null,"abstract":"<p><strong>Background: </strong>Alzheimer's disease (AD) is a neurodegenerative disease that is still incurable. Therapy with stem cell or extracellular vesicles is a promising strategy for AD treatment. Therefore, we evaluated whether stem cell-derived extracellular vesicles could improve cognitive function and pathological features in AD model mice.</p><p><strong>Methods: </strong>PubMed, Web of Science, Embase, and The Cochrane Library were searched for studies reporting stem cell-derived extracellular vesicles treatment of AD mice from the establishment of each database to 1st August 2023. SYRCLE was used to assess the risk of bias. The extracted data were analyzed using RevMan 5.4 and Stata 15 software.</p><p><strong>Results: </strong>19 studies were included in the analysis. Meta-analysis showed that treatment with stem cell-derived extracellular vesicles significantly improved cognitive performance of AD mice in the Morris water maze test and the novel object recognition test, reduced β-amyloid deposition, alleviated neuroinflammation and decreased levels of the proinflammatory cytokines and glial fibrillary acidic protein (GFAP) in the brain of AD mice. However, stem cell-derived extracellular vesicle did not affect the level of brain phosphorylated tau (p-Tau).</p><p><strong>Conclusions: </strong>stem cell-derived extracellular vesicles may promote the degradation of β-amyloid plaques in the brain, regulate immunity and protect nerves, which result in cognitive improvement in AD mice.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144060468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PLGA-hucMSC-Ex Ameliorates Colitis by Reversing Epithelial-mesenchymal Transition.","authors":"Ziyue Liu, Jintao Yuan, Lan Wang, Muhammad AzharUd Din, Yiqing Tian, Fei Mao","doi":"10.2174/011574888X344050250320233038","DOIUrl":"https://doi.org/10.2174/011574888X344050250320233038","url":null,"abstract":"<p><strong>Introduction: </strong>Human umbilical cord mesenchymal stem cell-derived exosomes (hucMSC-Exs) have been found to exhibit therapeutic effects on inflammatory bowel disease (IBD). However, due to the harsh environment of the gastrointestinal tract, exosomes, as a type of biological drug or carrier of bio-active substances, are still delivered by tail vein injection.</p><p><strong>Method: </strong>In this study, hucMSC-Ex were coated with poly (lactic-co-glycolic acid) (PLGA) polymer to form microparticles, PLGA-hucMSC-Ex, by double emulsion method.</p><p><strong>Results: </strong>The oral administration of PLGA-hucMSC-Ex particles alleviated inflammation in the mice model of IBD by reversing IBD-induced epithelial-mesenchymal transition (EMT).</p><p><strong>Conclusion: </strong>This provides an alternative to exploring IBD treatments, with potential clinical application to relieve IBD in patients.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144048648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regenerative Potential of Umbilical Cord Blood-derived Stromal Cells along with Phytosterol Campesterol in Wound Healing of a Rat Model.","authors":"Ahmad, Tahir Maqbool, Mahwish Arooj, Moutasem Salih Aboonq, Awais Altaf, Madaniah Omar Zakari, Moaz Abdullah Mojaddidi, Sajida Shahnawaz, Muzammal Mateen Azhar, Inamullah -","doi":"10.2174/011574888X345915250317063656","DOIUrl":"https://doi.org/10.2174/011574888X345915250317063656","url":null,"abstract":"<p><strong>Introduction: </strong>Mesenchymal stem cells derived from umbilical cord blood (UCB-MSCs) have a well-known role in fastening the wound healing process due to their less immune rejection, anti-inflammatory effects, and their role in cellular growth. Campesterol is a nutritional phytosterol with extensive health values and a competitor of cholesterol in the blood. Campesterol shares some anti-inflammatory effects via its regulation of inflammatory markers by inhibiting the proinflammatory cytokines (including TNF-α, TNF-α, and IL-6) levels.</p><p><strong>Method: </strong>The purpose of this study was to assess the ameliorative role of combined therapy (campesterol and UCB-MSCs) in wound healing without immune rejection. The study comprised both invitro and in-vivo experiments. In-vitro analysis included assessments of the cell viability of campesterol on UCBMSCs using MTT, crystal blue, trypan blue, and cell scratch assays. For in-vivo trials, superficial burn wounds were created on Sprague Dawley rats to evaluate the effects of campesterol, UCB-MSCs, and their combination on healing outcomes. Tissue regeneration progress in the wound vicinity was assessed using H&E staining and ELISA (inflammatory and growth markers) analysis.</p><p><strong>Result: </strong>Results of in-vitro experiments indicated that campesterol at concentrations of 10μg, 20μg, and 30μg demonstrated the most efficient cell viability. Moreover, a 30ug dose of campesterol along with UCBMSCs was further applied, leading to smooth and uncomplicated healing in the animal models. H&E staining showed nearly normal skin tissue while hematological and biochemical markers were near to control. Serum levels of tissue growth promoter factors, including VEGF and collagen- 3, were higher, and pro-inflammatory markers (such as TGF-β1, TNF-α, and IL-6) were lower at the same time.</p><p><strong>Conclusion: </strong>The results of the combined (MSCs and campesterol) therapy showed enhanced wound healing abilities. However, further studies are recommended to explore new aspects of this promising therapeutic approach of UCBSCs along with steroid derivative campesterol.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143782258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Human Dental-Pulp MSCs Modified by Double-genes on Wound Healing in Diabetic-Foot Model.","authors":"Guangchang Zhu, Hongfang Meng, Yuefeng Yang, Qian Yuwen, Yong Zhou, Menghu Han, Xia Xia, Shiwei Song","doi":"10.2174/011574888X363143250221015057","DOIUrl":"https://doi.org/10.2174/011574888X363143250221015057","url":null,"abstract":"<p><strong>Objectives: </strong>Diabetic foot (DF) poses a great challenge to us due to its poor therapeutic effect. To seek a new cure, the human dental pulp mesenchymal stem cells (hDP-MSCs) were modified by vascular endothelial growth factor A (VEGFA) and basic fibroblast growth factor (bFGF) (hEDP-MSCs) to investigate their curative effect on DF wound in animal models.</p><p><strong>Methods: </strong>Forty-eight rats with DF constructed with streptozotocin and ligation of femoral arteries, were randomly divided into six equal groups, which respectively received an intramuscular injection of normal saline (Control group), hDP-MSCs, VEGFA-modified hDP-MSCs, bFGF-- modified hDP-MSCs, hEDP-MSCs, and Ad.VEGF.FGF (Ad.FV). The tissues around DF wound were collected to investigate the level of CD31, alpha-smooth muscle actin (α-SMA), and cytokines. The expression of Notch1, Hes1, and CD105 were assessed via Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) after administration.</p><p><strong>Results: </strong>The hEDP-MSCs increased capillaries and decreased wound area (%). QRT-PCR showed that hEDP-MSCs over-expressed the mRNA of Notch1, hairy and enhancer of split 1 and CD105 in peri-wound tissue post-treatment. Meanwhile, the hEDP-MSCs expressed more CD31 and α-SMA than other groups. The hEDP-MSCs expressed more VEGFA and bFGF than hDP-MSCs, and yet less than Ad.FV. Compared with hDP-MSCs, the hEDP-MSCs down-regulated the expressions of interleukin-1 beta (IL-1β), interleukin (IL-6), and tissue necrosis factor α (TNF-a) post-treatment.</p><p><strong>Conclusion: </strong>This study highlights the curative effect of hEDP-MSCs in the wound healing process, and demonstrates the decisive function of hEDP-MSCs in promoting angiogenesis and reducing inflammation.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143545143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HuMenSCs Initiate the Uterus Stromal Decidualization in Mouse.","authors":"Yibing Han, Zhaoyi Li, Hongliu Shi, Yang Dong, Tai Ip Chan, Gang Xiao, Yi Zhun Zhu","doi":"10.2174/011574888X344947250221103039","DOIUrl":"https://doi.org/10.2174/011574888X344947250221103039","url":null,"abstract":"<p><strong>Introduction: </strong>Human menstrual blood stem cells (huMenSCs) appear to be pre-clinically safe but a controlled phase I clinical trial is required to determine safety for clinical applications.</p><p><strong>Method: </strong>HuMenSCs established from healthy donors were free of bacteria, mycoplasma, chlamydia, and endotoxin. P3 (passage 3) huMenSCs expressed the mesenchymal stem cell markers. P6 huMenSCs were developmental multipotential and could translocated into the uterine subepithelial stroma after intrauterine transplantation. After 10 and 15 passages, the huMenSCs kept normal karyotypes.</p><p><strong>Results: </strong>Gene expression showed that compared with the human umbilical cords mesenchymal stem cells (huMSCs), the huMenSCs affected the stromal cells more effectively. The huMenSCs possibly enhanced the stromal cell multiplication and \"decidualization\" process initiated by Igfbp1.</p><p><strong>Conclusion: </strong>Expression of Igfbp1, Atf3, Ptgs2, Hoxa10, Nr4a1, and Fox A2 were significantly increased in the stromal cells of the huMenSCs transplanted uterine.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143545144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Cross-sectional Study on Knowledge, Perception, and Willingness Among Saudi Population Towards Stem Cell Treatment and Banking: Associated Factors and their Predictive Abilities.","authors":"Nousheen Aslam, Rahaf Fares Alanzi, Zainab Mohammed Alobaid, Jumanah Basem Alhumood, Nouf Abdullah Almustafa, Nuzhat Banu, Mohammad Daud Ali, Sherihan Ahmed Ghosn, Wasim Ahmed, Ayaz Ahmed","doi":"10.2174/011574888X348048250220061137","DOIUrl":"https://doi.org/10.2174/011574888X348048250220061137","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to assess the knowledge, perception, and willingness of the Saudi population towards stem cell treatment and banking, the associated factors, and their predictive abilities.</p><p><strong>Methods: </strong>A cross-sectional study was conducted from September to December 2022 in Saudi Arabia using a structured bilingual, self-administered online survey to collect sociodemographic information and determine the knowledge and understanding, perception, and willingness of the general population. Bloom's cut-off points were used to distribute the scores into three categories, namely strong (80-100%), moderate (60-79%), and weak (<59%). Descriptive statistics were used to assess each domain, while t-tests, ANOVA, and binary logistic regression were used to assess factors influencing each domain and their predictive abilities.</p><p><strong>Results: </strong>The study conscripted 440 respondents, mainly females (70%) aged 18-24 years (56.1%), mostly single (44.3%), Saudi nationals (89%), and college graduates (56.6%). Social media (53.4%) was the primary source of information. 77.95% of respondents exhibited a low level of knowledge. Females, Saudi nationals, respondents with Islamic beliefs, college graduates, and those who received information from family physicians and social media had significantly better knowledge. 50.68% of respondents showed a strong perception. Non-Saudi participants, those who received information from family and friends, and respondents with a high prevalence of hypertension and diabetes in their families had a better perception. However, only 21.59% showed strong willingness toward stem cell treatment and banking, including Saudi nationals and college graduates. Gender was found to be a significant predictor for better knowledge and perception, while no sociodemographic variables significantly predicted willingness.</p><p><strong>Conclusion: </strong>This study emphasizes the need for increased awareness, educational campaigns, and targeted strategies considering various socio-demographic factors to improve the knowledge, perception, and willingness of the general population toward stem cell treatments and banking in Saudi Arabia.</p>","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143495104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}