Current stem cell research & therapy最新文献_第9页

Human Umbilical Cord Mesenchymal Stem Cell-derived Exosome Regulates Intestinal Type 2 Immunity. 人脐带间充质干细胞衍生的外泌体可调控肠道 2 型免疫。

Current stem cell research & therapy Pub Date : 2025-01-01 DOI: 10.2174/011574888X314032240429113240

Jiajun Wu, Zhen Yang, Daoyuan Wang, Yihui Xiao, Jia Shao, Kaiqun Ren

{"title":"Human Umbilical Cord Mesenchymal Stem Cell-derived Exosome Regulates Intestinal Type 2 Immunity.","authors":"Jiajun Wu, Zhen Yang, Daoyuan Wang, Yihui Xiao, Jia Shao, Kaiqun Ren","doi":"10.2174/011574888X314032240429113240","DOIUrl":"10.2174/011574888X314032240429113240","url":null,"abstract":"Aims: The aim of this study was to investigate the role of human umbilical cord mesenchymal stem cell-derived exosomes (hUCMSC-Exo) in regulating the intestinal type 2 immune response for either protection or therapy.Background: hUCMSC-Exo was considered a novel cell-free therapeutic product that shows promise in the treatment of various diseases. Type 2 immunity is a protective immune response classified as T-helper type 2 (Th2) cells and is associated with helminthic infections and allergic diseases. The effect of hUCMSC-Exo on intestinal type 2 immune response is not clear.Method: C57BL/6 mice were used to establish intestinal type 2 immune response by administering of H. poly and treated with hUCMSC-Exo before or after H. poly infection. Intestinal organoids were isolated and co-cultured with IL-4 and hUCMSC-Exo. Then, we monitored the influence of hUCMSC-Exo on type 2 immune response by checking adult worms, the hyperplasia of tuft and goblet cells Result: hUCMSC-Exo significantly delays the colonization of H. poly in subserosal layer of duodenum on day 7 post-infection and promotes the hyperplasia of tuft cells and goblet cells on day 14 post-infection. HUCMSC-Exo enhances the expansion of tuft cells in IL-4 treated intestinal organoids, and promotes lytic cell death.Conclusion: Our study demonstrates hUCMSC-Exo may benefit the host by increasing the tolerance at an early infection stage and then enhancing the intestinal type 2 immune response to impede the helminth during Th2 priming. Our results show hUCMSC-Exo may be a positive regulator of type 2 immune response, suggesting hUCMSC-Exo has a potential therapeutic effect on allergic diseases.","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":"302-316"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141083004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stem Cell Interventions in the Treatment of Alzheimer's Disease. 干细胞干预治疗阿尔茨海默病。

Current stem cell research & therapy Pub Date : 2025-01-01 DOI: 10.2174/011574888X308941240507050855

Shrishti Madhan, Anisha Mehta, Anushka Santoshkumar, Srisri Satishkarthik, K N Aruljothi

{"title":"Stem Cell Interventions in the Treatment of Alzheimer's Disease.","authors":"Shrishti Madhan, Anisha Mehta, Anushka Santoshkumar, Srisri Satishkarthik, K N Aruljothi","doi":"10.2174/011574888X308941240507050855","DOIUrl":"10.2174/011574888X308941240507050855","url":null,"abstract":"Alzheimer's disease (AD), an inexorable neurodegenerative ailment marked by cognitive impairment and neuropsychiatric manifestations, stands as the foremost prevailing form of dementia in the geriatric population. Its pathological signs include the aggregation of amyloid proteins, hyperphosphorylation of tau proteins, and the consequential loss of neural cells. The etiology of AD has prompted the formulation of numerous conjectures, each endeavoring to elucidate its pathogenesis. While a subset of therapeutic agents has displayed clinical efficacy in AD patients, a significant proportion has encountered disappointment. Notably, the extent of neural cell depletion bears a direct correlation with the disease's progressive severity. However, the absence of efficacious therapeutic remedies for neurodegenerative afflictions engenders a substantial societal burden and exerts a notable economic toll. In the past two decades, the realm of regenerative cell therapy, referred to as stem cell therapy, has unfolded as an avenue for the exploration of profoundly innovative approaches to treat neurodegenerative conditions. This promise is underpinned by the remarkable capacity of stem cells to remediate compromised neural tissue by means of cell replacement, to cultivate an environment conducive to regeneration, and to shield extant healthy neuronal and glial components from further degradation. Thus, this review aims to delve into the current knowledge of stem cell-based therapies and future possibilities in this domain.","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":"377-388"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140924096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Acute Kidney Injury Induced by Cisplatin in Mice is Exacerbated by the Conditioned Medium Derived from the Cultivation of Mesenchymal Stem Cells under Hypoxic Conditions. 缺氧条件下间充质干细胞培养的条件培养基可加重顺铂所致小鼠急性肾损伤。

IF 2.2

Current stem cell research & therapy Pub Date : 2025-01-01 DOI: 10.2174/011574888X321387240909111413

Andrey A Temnov, Alla N Sklifas, Mars G Sharapov, Roman S Fadeev, Elena V Klychnikova, Andrey D Dolbnya, Stanislav I Pekov, Igor A Popov

{"title":"The Acute Kidney Injury Induced by Cisplatin in Mice is Exacerbated by the Conditioned Medium Derived from the Cultivation of Mesenchymal Stem Cells under Hypoxic Conditions.","authors":"Andrey A Temnov, Alla N Sklifas, Mars G Sharapov, Roman S Fadeev, Elena V Klychnikova, Andrey D Dolbnya, Stanislav I Pekov, Igor A Popov","doi":"10.2174/011574888X321387240909111413","DOIUrl":"https://doi.org/10.2174/011574888X321387240909111413","url":null,"abstract":"Background: Acute Kidney Injury (AKI) is a severe complication of cisplatin-based chemotherapy. Thus, searching for novel therapeutic approaches to reduce system toxicity is vital for improving patient outcomes. The use of stem cells or the paracrine factors released by these cells during cultivation is currently being explored as a potential method for AKI prevention during chemotherapy. However, the conditions of stem cell cultivation considerably affect the composition of paracrine factors released by cells.Objective: In this study, we aimed to investigate the impact of paracrine factors derived from mesenchymal stem cells cultured under hypoxic conditions on the progression of AKI induced by cisplatin.Methods: AKI was induced in mice by intraperitoneal administration of cisplatin with the simultaneous injection of fractions of conditioned medium obtained from the cultivation of mesenchymal stem cells under hypoxic conditions. The survival rate of animals was assessed alongside qRTPCR implementation to assess gene expression of cytokines.Results: The total fraction of conditioned medium and >30 kDa fraction had no impact on cisplatin nephrotoxicity in mice. However, either subcutaneous or intraperitoneal administration of <30 kDa fraction of conditioned medium exacerbated animal mortality and led to severe damage to renal tissues. The effect was in a good correlation with KIM-1 and CDKN1A gene expression.Conclusion: The conditioned medium obtained during mesenchymal stem cells under hypoxic conditions has been found to markedly amplify the toxicity of cisplatin, which should be considered in stem cell therapy of AKI patients.","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":"20 7","pages":"784-794"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145380567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic Role of Bone Marrow Mesenchymal Stem Cells (BMSCs) in Nonalcoholic Steatohepatitis (NASH) Cirrhosis. 骨髓间充质干细胞（BMSCs）在非酒精性脂肪性肝炎（NASH）肝硬化中的治疗作用

IF 2.2

Current stem cell research & therapy Pub Date : 2025-01-01 DOI: 10.2174/011574888X328447241023063825

Hai-Tang Jiang, Hao-Ming Ye, Hua-Yang Yu, Yi-Ping Zhu, Ming-Yu Hu, Man-Li He, Wei-Yao Li, Ze-Hui Yu, Qian Yang, Lv-Qin He, Cong-Wei Gu

引用次数: 0

Simultaneous Co-transplantation for Highly Efficient Cell Therapy. 高效细胞治疗的同步共移植。

IF 2.2

Current stem cell research & therapy Pub Date : 2025-01-01 DOI: 10.2174/011574888X359983250408105711

Ji-Hee Choi, Mingu Ryu, Sung-Hwan Moon

{"title":"Simultaneous Co-transplantation for Highly Efficient Cell Therapy.","authors":"Ji-Hee Choi, Mingu Ryu, Sung-Hwan Moon","doi":"10.2174/011574888X359983250408105711","DOIUrl":"10.2174/011574888X359983250408105711","url":null,"abstract":"Cell therapy involves transplantation of cells to replace damaged tissues and cells and is used in regenerative medicine. Since its introduction, numerous cell therapy modalities have been developed to treat various diseases, and cell therapy has shifted the paradigm of the treatment of degenerative and refractory diseases. However, it faces limitations in terms of long-term therapeutic effects and efficiency. To overcome these challenges, the concept of co-transplantation, which utilizes two different cell sources, has been proposed. Stem cell-based co-transplantation approaches have been extensively studied both experimentally and clinically for various diseases, including graftversus- host disease (GVHD), infertility, acute liver failure (ALF), and myocardial infarction (MI). These have yielded improved transplantation efficiency and stability compared to single-cell transplantation methods. This review examines the development and effectiveness of co-transplantation through its application in four diseases. Additionally, it discusses the clinical applicability of cotransplantation, explores future research directions, and highlights its potential benefits.","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":"1144-1152"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12728527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stem Cell Infusion in Brain Cells to Reverse Aging. 干细胞注入大脑细胞逆转衰老。

Current stem cell research & therapy Pub Date : 2025-01-01 DOI: 10.2174/011574888X345183241014094641

Thangavel Lakshmipriya, Subash C B Gopinath

引用次数: 0

Molecular Mechanisms and Pathways of Mesenchymal Stem Cell-mediated Therapy in Brain Cancer. 间充质干细胞介导的脑癌治疗的分子机制和途径。

Current stem cell research & therapy Pub Date : 2025-01-01 DOI: 10.2174/011574888X341525250116052000

Kuldeep Singh, Geetanjali Singh, Jeetendra Kumar Gupta, Alka N Choudhary, Arun Kumar, Divya Jain, Mukesh Chandra Sharma, Shivendra Kumar

{"title":"Molecular Mechanisms and Pathways of Mesenchymal Stem Cell-mediated Therapy in Brain Cancer.","authors":"Kuldeep Singh, Geetanjali Singh, Jeetendra Kumar Gupta, Alka N Choudhary, Arun Kumar, Divya Jain, Mukesh Chandra Sharma, Shivendra Kumar","doi":"10.2174/011574888X341525250116052000","DOIUrl":"10.2174/011574888X341525250116052000","url":null,"abstract":"Mesenchymal stem cells (MSCs) have emerged as a promising therapeutic approach in the treatment of brain cancer due to their unique biological properties, including their ability to home tumor sites, modulate the tumor microenvironment, and exert anti-tumor effects. This review delves into the molecular mechanisms and pathways underlying MSC-mediated therapy in brain cancer. We explore the various signalling pathways activated by MSCs that contribute to their therapeutic efficacy, such as the PI3K/Akt, Wnt/β-catenin, and Notch pathways. Additionally, we discuss the role of exosomes and microRNAs secreted by MSCs in mediating anti-tumor effects. The review also addresses the challenges and future directions in optimizing MSC-based therapies for brain cancer, including issues related to MSC sourcing, delivery methods, and potential side effects. Through a comprehensive understanding of these mechanisms and pathways, we aim to highlight the potential of MSCs as a viable therapeutic option for brain cancer and to guide future research in this field.","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":"479-493"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143034762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to: The Renoprotective and Anti-Inflammatory Effects of Human Urine-Derived Stem Cells on Acute Kidney Injury Animals. 人尿源性干细胞对急性肾损伤动物的肾保护和抗炎作用的更正。

Current stem cell research & therapy Pub Date : 2025-01-01 DOI: 10.2174/1574888X20999250210163559

Yuanyuan Kuang, Chenyu Fan, Xiaojun Long, Jiajia Zheng, Yunsi Zeng, Yuhui Wei, Jiasheng Zhang, Shuangjin Yu, Tong Chen, Hehuan Ruan, Yi Wang, Ning Na, Yiming Zhou, Jiang Qiu

引用次数: 0

Probing the Mesenchymal Stem Cell Aging through In silico Assessment of Extracellular Vesicle-mediated miRNAs. 通过计算机评估细胞外囊泡介导的mirna来探测间充质干细胞衰老。

IF 2.2

Current stem cell research & therapy Pub Date : 2025-01-01 DOI: 10.2174/011574888X342545241202050636

Ningning Mi, Xibin Liu, Yuhua Gao, Chunyu Bai, Xiangchen Li

{"title":"Probing the Mesenchymal Stem Cell Aging through In silico Assessment of Extracellular Vesicle-mediated miRNAs.","authors":"Ningning Mi, Xibin Liu, Yuhua Gao, Chunyu Bai, Xiangchen Li","doi":"10.2174/011574888X342545241202050636","DOIUrl":"10.2174/011574888X342545241202050636","url":null,"abstract":"Introduction: During mesenchymal stem cell (MSCs) aging, a decrease in its proliferation and regenerative capacity occurs, which is implicated in human aging. The MSCs aging process is regulated by genetics, metabolism, the external environment, and various complex pathways.Method: The aging of MSCs during in vitro culture poses a major challenge for developing cell therapy aimed at combating human diseases and aging. To identify the contributing factors underlying MSCs aging, we obtained datasets of mRNA expression changes before and after aging from the Gene Expression Omnibus (GEO) database and datasets of extracellular vesicles (EVs) microRNAs (miRNAs) expression changes (GSE153752, GSE195634, and GSE226464). We conducted an indepth analysis to screen the correlation between EVs-miRNAs and MSCs aging.Result: Our analysis identified significant differences in the expression of hsa-miR-146a-5p, hsamiR- 432-5p, hsa-miR-7706, hsa-miR-409-3p, and hsa-miR-17-5p in EVs before and after MSCs aging. These differences arise from the post-MSCs aging activation of signaling pathways, such as FOXO and P53, which promote the expression of hsa-miR-146a-5p, hsa-miR-432-5p, hsa-miR-7706, hsa-miR-409-3p, and hsa-miR-17-5p.Conclusion: Subsequently, these miRNAs are transported to EVs upon binding to the RNA-binding proteins A2BP1, SFRS2, MBNL1, EIF4B, and ACO1. This study used the correlation between MSCs aging and specific EVs-miRNAs to predict MSCs aging during the culture process.","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":"882-914"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142820438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell Technology in Stem Cell Research. 干细胞研究中的单细胞技术。

Current stem cell research & therapy Pub Date : 2025-01-01 DOI: 10.2174/011574888X265479231127065541

Ali Golchin, Forough Shams, Faezeh Moradi, Amin Ebrahimi Sadrabadi, Shima Parviz, Shahriar Alipour, Parviz Ranjbarvan, Yaser Hemmati, Maryam Rahnama, Yousef Rasmi, Shiva Gholizadeh-Ghaleh Aziz

{"title":"Single-cell Technology in Stem Cell Research.","authors":"Ali Golchin, Forough Shams, Faezeh Moradi, Amin Ebrahimi Sadrabadi, Shima Parviz, Shahriar Alipour, Parviz Ranjbarvan, Yaser Hemmati, Maryam Rahnama, Yousef Rasmi, Shiva Gholizadeh-Ghaleh Aziz","doi":"10.2174/011574888X265479231127065541","DOIUrl":"10.2174/011574888X265479231127065541","url":null,"abstract":"Single-cell technology (SCT), which enables the examination of the fundamental units comprising biological organs, tissues, and cells, has emerged as a powerful tool, particularly in the field of biology, with a profound impact on stem cell research. This innovative technology opens new pathways for acquiring cell-specific data and gaining insights into the molecular pathways governing organ function and biology. SCT is not only frequently used to explore rare and diverse cell types, including stem cells, but it also unveils the intricacies of cellular diversity and dynamics. This perspective, crucial for advancing stem cell research, facilitates non-invasive analyses of molecular dynamics and cellular functions over time. Despite numerous investigations into potential stem cell therapies for genetic disorders, degenerative conditions, and severe injuries, the number of approved stem cell-based treatments remains limited. This limitation is attributed to the various heterogeneities present among stem cell sources, hindering their widespread clinical utilization. Furthermore, stem cell research is intimately connected with cutting-edge technologies, such as microfluidic organoids, CRISPR technology, and cell/tissue engineering. Each strategy developed to overcome the constraints of stem cell research has the potential to significantly impact advanced stem cell therapies. Drawing on the advantages and progress achieved through SCT-based approaches, this study aims to provide an overview of the advancements and concepts associated with the utilization of SCT in stem cell research and its related fields.","PeriodicalId":93971,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":"9-32"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139513924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0