{"title":"Evaluation of the analytic performance and macroprolactin sensitivity of a new prolactin immunoassay.","authors":"Guillaume David, Pauline Perrin, Camille Sergeant, Gérald Raverot, Véronique Raverot","doi":"10.1016/j.ando.2024.101677","DOIUrl":"https://doi.org/10.1016/j.ando.2024.101677","url":null,"abstract":"<p><strong>Purpose: </strong>Prolactin measurement is essential in endocrine diagnostics. Challenges such as the hook effect and reactivity to macroprolactin, which varies according to the reagent, complicate accurate measurement. The present study evaluated a newly marketed reagent to detect prolactin, IDS Prolactin, comparing it to an established reagent, Roche Elecsys Prolactin, assessing its behavior toward macroprolactin and polyethylene glycol (PEG) treatment, and establishing reference intervals.</p><p><strong>Methods: </strong>The IDS Prolactin and Roche Elecsys Prolactin assays were compared using 44 samples containing macroprolactin confirmed on gel filtration chromatography (macroprolactin: BBPRL) and 104 samples for which the diagnosis of macroprolactin was excluded (monomeric prolactin: MNPRL). Analytic performance of the IDS Prolactin assay was also assessed.</p><p><strong>Results: </strong>The new reagent showed satisfactory analytic performance, meeting EFLM standards for repeatability and intermediate imprecision. Comparison between the two methods found robust correlation for monomeric samples (y=1.060x-18.28; r2=0.993). Compared to the Roche assay, which is particularly low in its reaction to macroprolactin, the IDS assay displayed a higher level of detection. PEG precipitation effectively separated monomeric and macroprolactin samples when a cut-off of 65% recovery was used or at the threshold of 444 mIU/L (20.9 µg/L) for post-PEG monomeric prolactin upper limit of normal. Reference intervals were established for women, with ROC curve analysis demonstrating high sensitivity and specificity.</p><p><strong>Conclusion: </strong>The IDS Prolactin assay showed excellent analytic performance and satisfactory characteristics on macroprolactinemic samples.</p>","PeriodicalId":93871,"journal":{"name":"Annales d'endocrinologie","volume":" ","pages":"101677"},"PeriodicalIF":0.0,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142803802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Impact of Therapeutic Doses of Prednisolone and Other Glucocorticoids on Insulin Secretion from Human Islets.","authors":"Omolara Khadijat Tijani, Maria Moreno Lopez, Isaline Louvet, Ana Acosta-Montalvo, Anaïs Coddeville, Valery Gmry, Julie Kerr Conte, Francois Pattou, Marie-Christine Vantyghen, Chiara Saponaro, Caroline Bonner, Stéphanie Espiard","doi":"10.1016/j.ando.2024.101676","DOIUrl":"https://doi.org/10.1016/j.ando.2024.101676","url":null,"abstract":"<p><strong>Introduction: </strong>Glucocorticoid-induced diabetes (GCID) is a prevalent health issue, generally attributed to insulin resistance. High doses of dexamethasone (DEX) are known to inhibit glucose-stimulated insulin secretion (GSIS), but the effects of lower doses, commonly used in chronic therapy, and equipotent doses of other glucocorticoids (GCs) such as hydrocortisone (HC) and prednisone (PRED) remain underexplored. This study aimed to investigate these effects in vitro, and explore variations between patients.</p><p><strong>Materials and methods: </strong>Dynamic perifusion assays were conducted on human islets to evaluate the impact of different GCs on GSIS. The islets were treated for 24h with 250 nM PRED and other GCs at equipotent anti-inflammatory doses (HC: 1 µM; DEX: 38 nM).</p><p><strong>Results: </strong>In 11 human islet donor preparations, 250 nM PRED, corresponding to a clinical oral dose of 5mg/day, significantly inhibited the first and second phase of GSIS: area under the curve (AUC) decreased by 32.3% (p < 0.001), first phase by 41.5% (p < 0.001), and second phase by 38.4% (p < 0.001). Despite interindividual differences in GSIS response to PRED, no significant differences were observed according to body-mass index, gender or age. Comparing the effects of GCs at equipotent anti-inflammatory doses, DEX had a more pronounced inhibitory effect on GSIS than HC or PRED.</p><p><strong>Conclusions: </strong>In vitro, low-dose PRED treatment significantly impacted GSIS. DEX had a more unfavorable impact on GSIS than HC or PRED, indicating that metabolic effects do not align with anti-inflammatory potency.</p>","PeriodicalId":93871,"journal":{"name":"Annales d'endocrinologie","volume":" ","pages":"101676"},"PeriodicalIF":0.0,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yann Bertolani, Claudia García-Arumí, Tetiana Goncharova, Albert Arnaiz-Camacho, Jose García-Arumí
{"title":"Choroidal metastasis secondary to follicular thyroid carcinoma successfully managed with larotrectinib: A case report and review of the literature.","authors":"Yann Bertolani, Claudia García-Arumí, Tetiana Goncharova, Albert Arnaiz-Camacho, Jose García-Arumí","doi":"10.1016/j.ando.2024.11.001","DOIUrl":"10.1016/j.ando.2024.11.001","url":null,"abstract":"","PeriodicalId":93871,"journal":{"name":"Annales d'endocrinologie","volume":" ","pages":"101675"},"PeriodicalIF":0.0,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142756040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aysa Hacioglu, Emre Urhan, Zuleyha Karaca, Ahmet Selcuklu, Halil Ulutabanca, Okkes Celil Gokcek, Bilal Yekeler, Kursad Unluhizarci, Kaj Blennow, Henrik Zetterberg, Fahrettin Kelestimur
{"title":"Predictive value of neuronal markers for pituitary dysfunction following traumatic brain injury: A preliminary study.","authors":"Aysa Hacioglu, Emre Urhan, Zuleyha Karaca, Ahmet Selcuklu, Halil Ulutabanca, Okkes Celil Gokcek, Bilal Yekeler, Kursad Unluhizarci, Kaj Blennow, Henrik Zetterberg, Fahrettin Kelestimur","doi":"10.1016/j.ando.2024.10.003","DOIUrl":"https://doi.org/10.1016/j.ando.2024.10.003","url":null,"abstract":"<p><strong>Purpose: </strong>Traumatic brain injury (TBI), a well-known risk factor for pituitary dysfunction, is associated with increased serum neurofilament light chain (NFL), glial fibrillary acidic protein (GFAP), and total tau (t-tau) levels. We aimed to assess the predictive value of these markers and pituitary dysfunction following TBI in a prospective manner.</p><p><strong>Methods: </strong>Adult patients following TBI were included. Serum levels of NFL, GFAP, t-tau and pituitary and target hormones were analyzed prospectively during first week and one year after TBI.</p><p><strong>Results: </strong>Twenty-two patients (17 males, 5 females; mean age 40±15 years) were included in the study. Basal NFL levels correlated positively with length of hospital stay and basal cortisol (r=0.643, p=0.001 and r=0.558, p=0.007, respectively) and negatively with Glasgow Coma Scale (GCS) score and basal IGF-1 levels (r=-0.429, p=0.046 and r=-0.481, p=0.023, respectively), while there was no significant correlation between GFAP, t-tau and hormone levels. NFL, GFAP, and t-tau levels significantly decreased, and none of the patients developed hormone deficiencies one year after TBI. No correlations were detected between basal markers and first year pituitary hormone levels.</p><p><strong>Conclusion: </strong>Serum NFL levels were correlated with hormonal changes during acute phase of TBI reflecting the physiological response to trauma. Larger studies are needed to analyze the associations during chronic phase.</p>","PeriodicalId":93871,"journal":{"name":"Annales d'endocrinologie","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Philippe Oriot, Noemie Klipper Dit Kurz, Michel P Hermans
{"title":"Can standard CGM data be used to identify an HNF1B MODY-5 glucotype? Insights from a clinical case.","authors":"Philippe Oriot, Noemie Klipper Dit Kurz, Michel P Hermans","doi":"10.1016/j.ando.2024.10.002","DOIUrl":"https://doi.org/10.1016/j.ando.2024.10.002","url":null,"abstract":"","PeriodicalId":93871,"journal":{"name":"Annales d'endocrinologie","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142585045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maria Adriana Rangel, Rita Calejo, Vilma Lopes, Rosa Arménia Campos, Ana Luísa Leite
{"title":"Body composition in a pediatric population with type-1 diabetes - The importance of planned physical exercise.","authors":"Maria Adriana Rangel, Rita Calejo, Vilma Lopes, Rosa Arménia Campos, Ana Luísa Leite","doi":"10.1016/j.ando.2024.09.004","DOIUrl":"10.1016/j.ando.2024.09.004","url":null,"abstract":"<p><strong>Objective and methods: </strong>This study aimed to assess the body composition of pediatric patients with type-1 diabetes (T1D) in a Portuguese pediatric endocrinology/diabetic clinic, using the InBody 570 bioimpedance system. Preschool children (<6 years) and those recently diagnosed (<6 months) were excluded.</p><p><strong>Results: </strong>The study included 78 patients (53% female). Median age at assessment was 14 years, with 81% pubertal children. Eighty-seven percent were using continuous subcutaneous insulin infusion (CSII), 25% with an automated closed-loop model. Median HbA1c was 7.3%. Most had an adequate body mass index (BMI) standard deviation score (62%) and 48% engaged in regular physical exercise (PE) outside of school. Median percentage body fat (PBF) was 21.5% and was abnormal in 48%, with median visceral adiposity of 4. Despite adequate BMI, 11% had excessive PBF. PBF was significantly associated with visceral fat (r=0.79; P<0.001), female gender (P<0.001) and PE performed out of school (P=0.005). PE was associated with lower PBF (P=0.005), lower visceral fat (P=0.002), and higher muscle-to-fat ratio (P=0.006).</p><p><strong>Conclusion: </strong>Engaging in physical exercise out of school correlated significantly with improved body composition, characterized by reduced PBF and diminished visceral adiposity. Considering the known benefits of physical exercise for metabolic and glycemic control, this study highlights the importance of promoting regular physical exercise in T1D patients.</p>","PeriodicalId":93871,"journal":{"name":"Annales d'endocrinologie","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142303183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. Bellahreche, O. Sihali-Beloui, N. Semiane, A. Mallek, Y. Dahmani
{"title":"20-Hydroxyecdysone induces brown adipose tissue glycogen accumulation in obese gerbil model: Histochemical study","authors":"Z. Bellahreche, O. Sihali-Beloui, N. Semiane, A. Mallek, Y. Dahmani","doi":"10.1016/j.ando.2023.07.469","DOIUrl":"https://doi.org/10.1016/j.ando.2023.07.469","url":null,"abstract":"Obesity is a major public health problem. It is defined as an abnormal or excessive accumulation of fat harmful to health. Although the majority of obesity studies focus on changes in white adipose tissue, investigations into brown adipose tissue and its activation are becoming an increasingly popular area of interest. In this study, we attempted to examine the effects of 20-hydroxyecdysone (20E) on the glycogen amount stored in interscapular brown adipose tissue (IBAT). 15 gerbils were divided into: control group submitted to natural diet, two experimental groups submitted to high-calorie-diet supplemented or not with 20E. At the end of the experimental period, the animals were sacrificed and the IBAT was fixed in 10% formalin. Sections of 5 micrometers were made and stained with periodic acid-Schiff (PAS), to highlight the polysaccharides. Glycogen quantification in IBAT was performed using ImageJ software (NIH, Bethesda, MD, USA). PAS staining demonstrated that in the control gerbils, IBAT had strong cytoplasmic presence of glycogen. However, in the high-calorie diet group, the microscopy images had weaker PAS staining with lipid droplets infiltration compared to control group. We observed an important IBAT cytoplasm staining with PAS in group treated with 20E, indicating cells glycogen overload with reduction of lipid droplets accumulation. The quantification of glycogen had confirmed the qualitative study. Although the relationship between brown adipose tissue and glycogen storage remains underestimated and misunderstood, these preliminary results show that 20E could activate obese gerbil's brown adipocytes to store more glycogen.","PeriodicalId":93871,"journal":{"name":"Annales d'endocrinologie","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135274371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"L’hémiballisme secondaire à l’hyperglycémie non cétosique : une situation rare","authors":"Z. Habbadi, I. Gam","doi":"10.1016/j.ando.2023.07.455","DOIUrl":"https://doi.org/10.1016/j.ando.2023.07.455","url":null,"abstract":"Le diabète déséquilibré peut entraîner des complications neurologiques comme des crises épileptiques et des troubles de conscience. L’hémiballisme est une manifestation plus rare. Le mécanisme expliquant la survenue de ces mouvements anormaux lors d’une hyperglycémie sans cétose reste mal élucidé. Patiente de 67 ans, hypertendue sous traitement, diabétique de type 2 depuis 4 ans sous association metformine et insuline. Admise aux urgences neurologiques pour des mouvements anormaux de l’hémicorps droit d’apparition brutale. Ces mouvements prédominent au niveau proximal, d’abord au niveau du membre supérieur droit, puis au membre inférieur homolatéral à type de flexion extension et de rotation. Ils sont brusques et irréguliers altérant sa qualité de vie. Le bilan étiologique révèle une hyperglycémie à 5 g/L sans hyperosmolarité ni cétonurie ni syndrome infectieux associé. La TDM cérébrale montre une atteinte du striatum gauche. L’évolution était marquée par la disparition de l’hémiballisme deux semaines après l’équilibre glycémique et la mise sous neuroleptique à faible dose. L’hémiballisme est un mouvement unilatéral rapide, brutal, non rythmique, non suppressible, dû à une atteinte du striatum controlatéral. Bien que rare, l’hyperglycémie non cétosique est l’une de ses causes. Dans ce cadre, plusieurs hypothèses sont avancées. En effet, l’épuisement de l’acide gamma-aminobutyrique et de l’acétate au cours de l’hyperglycémie sans cétose, avec le manque d’énergie et l’acidose métabolique, serait à l’origine de l’apparition des mouvements anormaux. Le diagnostic est radiologique et le traitement repose sur l’équilibre glycémique.","PeriodicalId":93871,"journal":{"name":"Annales d'endocrinologie","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135274381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. Ben Hilel, I. Rojbi, S. Mekni, S. Essayeh, K. Khiari, I. Ben Nacef
{"title":"Cardiothyréose : à propos de neuf cas","authors":"W. Ben Hilel, I. Rojbi, S. Mekni, S. Essayeh, K. Khiari, I. Ben Nacef","doi":"10.1016/j.ando.2023.07.254","DOIUrl":"https://doi.org/10.1016/j.ando.2023.07.254","url":null,"abstract":"La cardiothyréose, complication majeure de l’hyperthyroïdie peut révéler ou survenir après le diagnostic la maladie. L’objectif de ce travail est de décrire le profil clinique, paraclinique et évolutif de la cardiothyréose. Il s’agissait d’une étude rétrospective descriptive réalisée à partir des patients hyperthyroïdiens hospitalisés dans notre service entre 2008 et 2022. Le diagnostic de cardiothyréose était retenu devant un trouble de rythme, une insuffisance cardiaque ou coronaire. Au total, 4 % des patients hyperthyroïdiens avaient une cardiothyréose (9/227). L’âge moyen était 55 ± 12 ans avec un sexe ratio = 2. La fréquence cardiaque moyenne était à 166 ± 32. L’amaigrissement et les palpitations étaient les signes prédominants retrouvés chez huit patients. L’hyperthyroïdie était fruste chez un patient. Le taux moyen de TSH était à 0,032 ± 0,02 U/ml et de FT4 à 3,5 ± 1,96 ng/dl [VN : 0,7–1,48]. Huit patients avaient une maladie de Basedow. L’arythmie cardiaque par fibrillation atriale (ACFA) était objectivée chez tous les patients et révélatrice de l’hyperthyroïdie dans trois cas. L’association ACFA et insuffisance cardiaque étaient présente chez deux patients. L’ETT montrait Une FEVG inférieure à 50 % et une HTAP dans deux cas. Le bisoprolol a été prescrit chez cinq patients. Parmi les six patients suivis après normalisation de la FT4, on obtenait la conversion de l’ACFA chez trois. La cardiothyréose est une complication sérieuse de l’hyperthyroïdie pouvant menacer le pronostic vital. L’ACFA est la forme clinique la plus fréquente et le retour au rythme sinusal n’est pas constant après contrôle de l’hyperthyroïdie, d’où l’intérêt du diagnostic et de prise en charge précoce de l’hyperthyroïdie.","PeriodicalId":93871,"journal":{"name":"Annales d'endocrinologie","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135274386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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