Jafar Fathi-Qarachal, S. Ali Moosawi-Jorf*, Mansoor Karimi-Jashni and Maryam Nikkhah,
{"title":"Impact of Combining Chitosan Nanoparticles and Cyproconazole in Controlling Wheat Stem Rust","authors":"Jafar Fathi-Qarachal, S. Ali Moosawi-Jorf*, Mansoor Karimi-Jashni and Maryam Nikkhah, ","doi":"10.1021/acsagscitech.5c00191","DOIUrl":"https://doi.org/10.1021/acsagscitech.5c00191","url":null,"abstract":"<p >Wheat stem rust, caused by <i>Puccinia graminis</i> f. sp. <i>tritici</i>, is a devastating disease that inflicts significant global damage annually. To combat this disease, chitosan nanoparticles (CNPs) were combined with the fungicide cyproconazole (CYP) and applied as a foliar spray 24 h postinoculation (hpi) with fungal urediniospores. Plant samples were analyzed to assess fungal progression by examining pustule formation and quantifying fungal DNA content in leaf tissues. Treatment with CNPs and the cyproconazole-chitosan nanocomposite (C-CYP) resulted in significantly smaller pustules or their absence and reduced fungal DNA levels compared to controls. Additionally, enzyme assays revealed that the activities of peroxidase, catalase, polyphenol oxidase, and phenylalanine ammonia-lyase significantly increased 24 h post-treatment with CNPs compared to controls. Furthermore, transcription levels of WAK-2, NPR-1, and Chitinase genes were notably upregulated in plants treated with CNPs and C-CYP at 24 h post-treatment. These findings suggest that the combination of chitosan nanoparticles and cyproconazole not only effectively controls wheat stem rust but also reduces environmental hazards by requiring lower chemical dosages.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 7","pages":"1413–1424"},"PeriodicalIF":2.9,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144807923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jaume Béjar-Grimalt, David Pérez-Guaita*, Ángel Sánchez-Illana*, Rodolfo García-Contreras, Rashmi Kataria, Sylvie Bureau, Miguel de la Guardia and Frédéric Cadet,
{"title":"Classification of Apricot Varieties by Infrared Spectroscopy and Machine Learning","authors":"Jaume Béjar-Grimalt, David Pérez-Guaita*, Ángel Sánchez-Illana*, Rodolfo García-Contreras, Rashmi Kataria, Sylvie Bureau, Miguel de la Guardia and Frédéric Cadet, ","doi":"10.1021/acsagscitech.5c00068","DOIUrl":"10.1021/acsagscitech.5c00068","url":null,"abstract":"<p >This work aimed to investigate using ATR–FTIR spectroscopy combined with machine learning to classify eight apricot varieties. Traditionally, variety identification relies on physicochemical property measurements, which are time-consuming and require laboratory analysis. Instead, we used the ATR–FTIR spectra from 731 apricots divided into calibration (512) and test (219) sets and three machine learning models (i.e., partial least-squares-discriminant analysis (PLS-DA), support vector machine (SVM), and random forest (RF)) to accurately predict 97% of the test samples. Additionally, careful inspection of the PLS-DA regression vectors revealed a strong correlation between the spectra and biochemical composition in sugar and organic acids, validating ATR–FTIR spectroscopy as a viable alternative for variety identification. Finally, to validate the results, additional models were constructed using the physicochemical data from the apricots. These reference models were then tested using the same data splits as the spectroscopic data used as a reference method, obtaining similar results with both approaches.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 7","pages":"1373–1381"},"PeriodicalIF":2.9,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12309246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144755345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Poonam Jyoti*, Jyoti Bhardwaj, Girija Kaushal and Sudesh Kumar Yadav*,
{"title":"Advancing Our Understanding of Plant–Microbe Interactions through Integrating Multiomics and Stable Isotopes for Sustainable Agriculture","authors":"Poonam Jyoti*, Jyoti Bhardwaj, Girija Kaushal and Sudesh Kumar Yadav*, ","doi":"10.1021/acsagscitech.5c00098","DOIUrl":"https://doi.org/10.1021/acsagscitech.5c00098","url":null,"abstract":"<p >Both plants and microbes share a vital relationship. Microbes are strong drivers of plant adaptation and evolution, as they impact plant growth, facilitate nutrient cycling, and influence susceptibility or tolerance to both abiotic and biotic stress conditions. Similarly, plants also influence microbial colonization and help establish microbial consortia in the rhizosphere through exuded metabolites and molecular signals. These plant–microbe relationships have a significant role in agriculture and soil health, but understanding them is difficult because of the complexity and variability of the rhizosphere. However, recent omics developments offer new opportunities to delve deeper into these complex communities. This review focuses on integrating multiomics methods and stable isotopes to gain a better idea of how plants and microbes interact, which will help in the better utilization of beneficial interactions for plant growth and productivity.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 7","pages":"1225–1237"},"PeriodicalIF":2.9,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144807716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lydia Rubilar, Javiera Avilés, Michelle Sarmiento, Felipe Sobarzo, Gustavo E. Zúñiga* and Rodrigo A. Contreras*,
{"title":"Priming of C-Glycoside Flavones in Colobanthus quitensis with Salicylic Acid, Methyl Jasmonate, Pimelic Acid, Suberic Acid, and Azelaic Acid Elicits Antifungal Activity against Botrytis cinerea","authors":"Lydia Rubilar, Javiera Avilés, Michelle Sarmiento, Felipe Sobarzo, Gustavo E. Zúñiga* and Rodrigo A. Contreras*, ","doi":"10.1021/acsagscitech.5c00273","DOIUrl":"https://doi.org/10.1021/acsagscitech.5c00273","url":null,"abstract":"<p ><i>Colobanthus quitensis</i>, one of only two native angiosperms in Antarctica, produces C-glycosyl flavones with antifungal activity against <i>Botrytis cinerea</i>. In this study, the exogenous application of the elicitors salicylic acid (SA), methyl jasmonate (MeJA), pimelic acid (PA), suberic acid (SuA), and azelaic acid (AzA) was evaluated for its effect on the accumulation of bioactive metabolites in in vitro-cultivated plants. Exposure to these compounds significantly modulated the expression of key genes in the phenylpropanoid and flavonoid pathways, including <i>pal</i>, <i>chs</i>, <i>chi</i>, and <i>fnsII</i>, as well as regulatory genes such as <i>myb12</i>, <i>bhlh</i>, and <i>wrky33</i>, enhancing PAL activity and the accumulation of C-glycosyl flavones including schaftoside, neoschaftoside, saponarin, and swertiajaponin. This priming process improved the antifungal activity of the extracts, with SuA at 75 μM and MeJA at 50 μM were identified as the most effective treatments. The in vitro culture approach enabled the assessment of protected and hard-to-access species without the need for wild harvesting. These results suggest that the exogenous application of elicitors constitutes an efficient strategy to modulate the biosynthesis of specialized metabolites, with implications for the development of biocontrol agents and the improvement of efficiency in sustainable agricultural systems.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 7","pages":"1497–1509"},"PeriodicalIF":2.9,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144807597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
María F. Salcedo, Florencia A. Mesas, Andrés Torres Nicolini, Felipe del Pazo, Gabriel M. Briscioli, Claudia A. Casalongué and Andrea Y. Mansilla*,
{"title":"Valuable Properties of Chitosan-Enriched Extracts from Black Soldier Fly Pupal Exuviae as Plant Biostimulants","authors":"María F. Salcedo, Florencia A. Mesas, Andrés Torres Nicolini, Felipe del Pazo, Gabriel M. Briscioli, Claudia A. Casalongué and Andrea Y. Mansilla*, ","doi":"10.1021/acsagscitech.5c00050","DOIUrl":"https://doi.org/10.1021/acsagscitech.5c00050","url":null,"abstract":"<p >Chitin, a prevalent polysaccharide in fungal cell walls, insect exoskeletons, and crustacean shells, is traditionally extracted from crustacean shells. However, aligning with circular economy principles, there is growing interest in utilizing bioconverter insects like the black soldier fly (<i>Hermetia illucens</i>). We explored the potential of <i>H. illucens</i> pupal grown on discarded agro-industrial waste to obtain enriched chitosan sources for plant biostimulants and protectors. Chitin was extracted from the pupal exuviae, and the properties of bleached (ChB) and nonbleached (ChU) chitosan fractions were compared. Both fractions exhibited structural similarities to commercial chitosan, with ChU showing a superior antioxidant capacity. In hydroponic lettuce cultivation, ChU significantly increased the biomass and chlorophyll content, while ChB had no notable effect. Both ChU and ChB activated auxin signaling in a transgenic tomato model and exhibited fungicidal activity against <i>Fusarium solani</i> f. sp. <i>eumartii</i>. These findings indicate that <i>H. illucens</i> pupal exuviae, cultivated on discarded agro-industrial waste, offer a valuable alternative for use as a plant biostimulant. This type of research forms the foundational basis for recognizing the potential of waste as a resource to innovate and create various products aligned with the circular economy strategy.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 8","pages":"1619–1631"},"PeriodicalIF":2.9,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144860205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Nano-Hydroxyapatite Improves Drought Resilience in Soybean: Coordinated Physiological Adaptation and Soil Phosphorus Management for Sustainable Cropping Systems","authors":"Tianyu Zhang, Wenxuan Zhang, Qile Fang, Yungui Li, Zihao Zhao, Chuanxin Ma, Yuan Gao and Qingqing Li*, ","doi":"10.1021/acsagscitech.5c00217","DOIUrl":"https://doi.org/10.1021/acsagscitech.5c00217","url":null,"abstract":"<p >Under global warming scenarios, where drought and water scarcity are becoming more prevalent, this study investigated the efficacy of the nanophosphorus material (n-P) in enhancing soybean resilience under soil water restriction. Compared to conventional ionic phosphate fertilizer (i-P), n-P application significantly improved soybean growth under water-limited conditions, achieving a near-normal growth status. The n-P treatments increased plant fresh weight by 21.2–30.2% relative to i-P, demonstrating superior growth promotion. Physiological analysis revealed that n-P enhanced stress adaptation through the modulation of antioxidant enzyme activities, indicating improved oxidative stress management. Importantly, n-P application boosted reproductive success with 62.0% higher pod fresh weight and 21.5% increased total protein content compared to i-P. Postharvest soil analysis showed additional benefits of n-P fertilization, including 69% lower residual Olsen-P and ameliorated soil acidification compared with the i-P group. These findings provide mechanistic insights into nanofertilizer-mediated plant stress adaptation and highlight the dual benefits of n-P in sustainable crop production and soil health maintenance.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 7","pages":"1442–1451"},"PeriodicalIF":2.9,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144806665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Daryush Talei*, Ali Shams and Mojtaba Khayam Nekouei,
{"title":"A Comprehensive Review of Cannabis as a Crucial Pharmaceutical Plant and Its Efficient Propagation Methods","authors":"Daryush Talei*, Ali Shams and Mojtaba Khayam Nekouei, ","doi":"10.1021/acsagscitech.4c00483","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00483","url":null,"abstract":"<p >Agricultural products have been a very important factor in transforming humankind from hunter-gatherer to farmer throughout human civilization. In this regard, the production of various crops with therapeutic and nutritional values has been of interest in different societies. Industrialization of agriculture, limitation of natural resources, and population growth highlighted the importance of producing valuable species of medicinal plants. Cannabis is considered a medicinal plant, whose proliferation and mass production have attracted a lot of attention in recent years. The demand for the use of cannabis in the pharmaceutical and food industries is increasing significantly. Various methods of propagation and mass production have accordingly become the most important medicinal aspects of cannabis production. Despite the current achievements in improving cannabis production through the introduction of new varieties and different propagation methods, the development of large-scale micropropagation and modern breeding strategies, as well as the genetic conservation of this plant, should be given more attention. However, the application of plant biotechnologies requires high-throughput in vitro culture systems that enable the transformation and development of new cannabis varieties on a large scale. The present review summarizes the advantages and disadvantages of common methods of sexual and asexual propagation of cannabis seedlings.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 7","pages":"1191–1214"},"PeriodicalIF":2.9,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144809019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Applications and Challenges of Pullulan in China’s Food Industry","authors":"Zilong Zhao, Jing Chen* and Weigang Yuwen, ","doi":"10.1021/acsagscitech.5c00447","DOIUrl":"https://doi.org/10.1021/acsagscitech.5c00447","url":null,"abstract":"","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 7","pages":"1263–1265"},"PeriodicalIF":2.9,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144808862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thomas K. Porter, Gabriel Sánchez-Velázquez and Michael S. Strano*,
{"title":"The Role of Basal H2O2 Concentration in ROS Stress Signaling Waveforms In Planta","authors":"Thomas K. Porter, Gabriel Sánchez-Velázquez and Michael S. Strano*, ","doi":"10.1021/acsagscitech.5c00206","DOIUrl":"https://doi.org/10.1021/acsagscitech.5c00206","url":null,"abstract":"<p >In response to stress, living plants propagate a chemical wave composed of H<sub>2</sub>O<sub>2</sub> through their tissues. Advances in nanosensors capable of measuring H<sub>2</sub>O<sub>2</sub> within the living plant in real time have informed a quantitative theory to describe the spatiotemporal profile of its concentration─labeled a signaling <i>waveform</i>. A heretofore unaddressed aspect of the theory is the role of the existing basal H<sub>2</sub>O<sub>2</sub> concentration level within the plant before and after stress wave propagation, potentially informing mechanisms of stress priming─or state changes associated with prior, low magnitude levels of stress that condition the resulting waveform. Herein, we develop a mathematical description of wave propagation within an existing basal level of H<sub>2</sub>O<sub>2</sub>. We show that the shape and intensity of the waveform can be mathematically decoupled from the basal H<sub>2</sub>O<sub>2</sub> concentration. This opens the possibility that the equilibrium basal concentration can operate as a distinct, orthogonal signaling channel, separate from the acute waveform following a discrete stress event. The mathematics developed herein may find utility in a more detailed description of mechanisms such as stress priming. More broadly, the results will aid in extending waveform analysis across diverse plant species and environmental conditions.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 7","pages":"1434–1441"},"PeriodicalIF":2.9,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144809009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Manisha Parashar, Maniraj Rathinam, Narasimham Dokka and Rohini Sreevathsa*,
{"title":"Chemical Camouflage in Plants: Defensive Science of Stealth against Insect Herbivory","authors":"Manisha Parashar, Maniraj Rathinam, Narasimham Dokka and Rohini Sreevathsa*, ","doi":"10.1021/acsagscitech.5c00223","DOIUrl":"https://doi.org/10.1021/acsagscitech.5c00223","url":null,"abstract":"<p >Chemical camouflage is a fascinating and intricate survival strategy that plants have evolved to defend themselves against herbivores and pests. While plant defense mechanisms have long captivated scientific interest, their full potential remains largely untapped in practical applications. This review takes a distinctive interdisciplinary approach, weaving together insights from molecular biology, ecology, and agricultural science to offer a comprehensive perspective on chemical camouflage. It was driven by a shared understanding that integrating plant defense strategies more deeply into agricultural systems could help address urgent challenges such as pest control, crop productivity, and environmental sustainability. With continued research and advances in biotechnology, chemical camouflage could become a key component of future farming─where crops not only protect themselves but also contribute actively to a more productive and sustainable agricultural landscape.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 7","pages":"1251–1262"},"PeriodicalIF":2.9,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144808737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}