ACS agricultural science & technology最新文献

{"title":"Formulation of Dolomite-Supported and CNF-Translocated Multi-Micronutrients: An Advanced Fertilizer for Plant Growth","authors":"Rahul Gupta,&nbsp;Naveen Kumar Verma and Nishith Verma*,&nbsp;","doi":"10.1021/acsagscitech.5c0001310.1021/acsagscitech.5c00013","DOIUrl":"https://doi.org/10.1021/acsagscitech.5c00013https://doi.org/10.1021/acsagscitech.5c00013","url":null,"abstract":"<p >A novel dolomite-supported carbon nanofiber (CNF) formulation, loaded with copper, zinc, molybdenum, and boron micronutrients (MO-BO–CNF/dolomite) has been developed as a micronano fertilizer. Dolomite serves not only as a substrate for CNF-growth but also as the source of secondary nutrients (calcium and magnesium), which are essential for plant health. CNFs efficiently translocate micronutrients from plant roots to shoots and leaves. The synthesized formulation is characterized using various physicochemical techniques, including transmission electron microscopy, scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction (XRD), X-ray photoelectron spectroscopy, atomic absorption spectroscopy, and particle size analysis. The average CNF diameter is determined to be ∼107.2 nm, while XRD and X-ray photoelectron spectroscopies confirm the formation of metal oxides and CNFs. The formulation shows a surface charge of −19.4 mV, with a specific surface area of approximately 109 m²/g and total pore volume of 0.121 cc/g. Seed germination assays on <i>Cicer arietinum</i> with MO-BO–CNF/dolomite (1 mg/mL) over 6 days show 95% germination percentage. The 30-day plant growth experiment performed on <i>C. arietinum</i> plants using a dose of 1 g of the fertilizer per kg of soil reveals a significant improvement in the key growth parameters including the root and shoot lengths, biomass, and chlorophyll and protein contents in plants. The formulation improves not only the nutrient delivery efficiency but also the water-holding capacity of soil. MO-BO–CNF/dolomite-treated soil show a 7.8-fold rise in total nitrogen, underscoring its role in soil fertility. A relatively higher abundance of <i>Bacillus</i> sp<i>.</i>, <i>Acetobacter</i> sp<i>.</i>, and <i>Rhizobiales</i> facilitates nitrogen fixation in the MO-BO–CNF/dolomite treatment group. A controlled release of micronutrients offers a promising avenue for boosting modern agricultural productivity and sustainability.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 6","pages":"1057–1070 1057–1070"},"PeriodicalIF":2.3,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methoxychalcones and Cinnamaldehyde as Herbicidal Compounds","authors":"Raphael M. Garrido*,&nbsp;Franck E. Dayan,&nbsp;Patrick R. Ozanique,&nbsp;Luis O. Regasini and Rosana M. Kolb,&nbsp;","doi":"10.1021/acsagscitech.5c0005610.1021/acsagscitech.5c00056","DOIUrl":"https://doi.org/10.1021/acsagscitech.5c00056https://doi.org/10.1021/acsagscitech.5c00056","url":null,"abstract":"<p >Synthetic compounds inspired by natural products provide innovative herbicide leads. Here, 18 methoxychalcones, inspired by cinnamaldehyde and curcumin, were designed, synthesized, and evaluated for their herbicidal activity. Additionally, cinnamaldehyde and curcumin were also assessed. Laboratory and greenhouse bioassays evaluated the inhibition caused by the compounds on initial growth in lettuce and weeds. In laboratory settings, 4’-hydroxy-3′-methoxychalcone induced the highest inhibition for lettuce root (66%) and shoot length (53%) compared to the negative control. The compounds 4’-hydroxy-3′-methoxychalcone and cinnamaldehyde inhibited about 87% of <i>Urochloa decumbens</i> growth, making them the most effective in reducing initial weed growth. Chalcones displayed no selectivity, inhibiting the growth of monocotyledonous and eudicotyledonous species. Additionally, 4’-hydroxy-3′-methoxychalcone caused 50% injury in <i>Raphanus raphanistrum</i> under greenhouse conditions. Chalcones and cinnamaldehyde hold promise for developing herbicides. Additional studies assessing the combined effects of methoxychalcones would be valuable to determine whether their herbicidal activity can be intensified.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 6","pages":"1086–1095 1086–1095"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsagscitech.5c00056","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing the Shelf Life and Stress Tolerance of the Biocontrol Agent Trichoderma harzianum by Encapsulation in Green Matrices of Nanocellulose and Carboxymethyl Cellulose","authors":"Mariana G. Brondi,&nbsp;Camila Florencio,&nbsp;Vanessa M. Vasconcellos,&nbsp;Caue Ribeiro and Cristiane S. Farinas*,&nbsp;","doi":"10.1021/acsagscitech.5c0018910.1021/acsagscitech.5c00189","DOIUrl":"https://doi.org/10.1021/acsagscitech.5c00189https://doi.org/10.1021/acsagscitech.5c00189","url":null,"abstract":"<p >Microbial inoculants offer a promising solution for reducing the environmental impact of agrochemicals while enhancing crop productivity within a bioeconomy framework. However, extending the shelf life and enhancing the stability of these beneficial microorganisms are crucial for making these biological solutions viable alternatives to chemical fertilizers and pesticides. In this study, we developed biobased encapsulation matrices using cellulose nanocrystals (CNC) and a composite of CNC and carboxymethyl cellulose (CNC:CMC) to encapsulate spores of the biocontrol fungus <i>Trichoderma harzianum</i>. Our findings revealed that encapsulation significantly increased the microorganism shelf life. After 1 year, approximately 10⁸ CFU/mL of the initial 10⁹ CFU/mL encapsulated spores remained viable, while nearly all free spores were no longer viable. Encapsulation also improved the microorganism resistance to stressful conditions, such as heat, UV radiation, and chemical fungicide exposure. Specifically, the CNC and CNC:CMC matrices maintained up to 4.7 × 10⁸ CFU/mL after fungicide exposure. Furthermore, encapsulation preserved the antagonistic activity of <i>T. harzianum</i> against the phytopathogen <i>Fusarium solani</i> for up to 1 year. These results demonstrate the potential of cellulose-based matrices for developing microbial inoculant formulations that support the shift toward more sustainable agricultural practices.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 6","pages":"1178–1188 1178–1188"},"PeriodicalIF":2.3,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsagscitech.5c00189","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Variability in the Methylxanthine Content within Ilex guayusa Loes: Impact of Soil Conditions, Age, and Sunlight Exposure","authors":"Mateo A. Fernández,&nbsp;Melanie Ochoa-Ocampo,&nbsp;Thomas Garzón,&nbsp;Karla Martinez,&nbsp;Iverson Sinaluisa,&nbsp;Jefferson V. Pastuña-Fasso,&nbsp;Nina Espinosa de los Monteros-Silva,&nbsp;Zulay Niño-Ruíz,&nbsp;Noroska G. S. Mogollón* and Karel Diéguez-Santana*,&nbsp;","doi":"10.1021/acsagscitech.4c0077810.1021/acsagscitech.4c00778","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00778https://doi.org/10.1021/acsagscitech.4c00778","url":null,"abstract":"<p >The Amazon rainforest is a highly biodiverse region, offering valuable plants for the pharmaceutical, cosmetic, and food industries. <i>Ilex guayusa</i>, from the Ecuadorian Amazon, is rich in methylxanthines, compounds with stimulant effects and health benefits. This study evaluates the variability in the methylxanthine content within <i>I. guayusa</i> under different soil, light, and plant age conditions, aiming to understand how these cultivation variables affect the production of these compounds and how they can be optimized through sustainable agricultural practices. A mixed-level factorial design analyzed leaf samples, considering plant age, soil quality, and light conditions. Using HPLC-UV and LC-MS/MS techniques, the results show that shaded conditions increase methylxanthine accumulation, with caffeine and theobromine concentrations rising with leaf age, while theophylline decreases under light. Soil with high organic matter and nutrients enhanced methylxanthine levels. These findings suggest shade management and optimal harvest timing are key to maximizing methylxanthine production for future industrial applications.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 6","pages":"1034–1046 1034–1046"},"PeriodicalIF":2.3,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Double-Encapsulated Controlled Release System for the Staged Delivery of a Growth Stimulant and Herbicide","authors":"Yogeshwar P. Aher,&nbsp;Benu Adhikari,&nbsp;Ravi Shukla,&nbsp;Marimuthu S* and Kadhiravan Shanmuganathan*,&nbsp;","doi":"10.1021/acsagscitech.4c0078410.1021/acsagscitech.4c00784","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00784https://doi.org/10.1021/acsagscitech.4c00784","url":null,"abstract":"<p >Weed control remains a critical challenge in agriculture, especially with parasitic weeds such as Orobanche spp., <i>Striga</i> spp., and <i>Cuscuta</i> spp., which significantly reduce crop yield. Although herbicides are effective against common weeds, they are less effective against parasitic weeds due to their unique germination mechanisms and close association with host plants. Growth regulators can stimulate the germination of parasitic weeds (suicidal germination) in the absence of host plants, providing a potential opportunity for controlling parasitic weeds, especially <i>Striga</i> sps. We report here a double-encapsulated staged delivery system, wherein herbicide (atrazine) is encapsulated in the inner polyurea shell and a growth stimulant (Gibberellic acid, GA3) in the outer alginate matrix. In vitro release study conducted in an aqueous medium showed that the time required for 50% release of atrazine from this double-encapsulated system is 96 h against 3 h for GA3. The quick release of GA3, followed by a slow and sustained release of atrazine, is expected to first induce the germination of parasitic weed seeds using GA3 and subsequently exterminate them upon exposure to atrazine. Interpretation of release data using the Weibull model led to the conclusion that the release of atrazine and GA3 is primarily governed by Fickian diffusion. Soil breakthrough profile shows that double encapsulation of atrazine helps to control the overleaching of atrazine into the soil.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 6","pages":"1047–1056 1047–1056"},"PeriodicalIF":2.3,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Chlorella vulgaris and Probiotic Dietary Supplementation on Performance, Apparent Digestibility, and Gut Morphology of Post-weaning Piglets","authors":"Miguel F. Pedro,&nbsp;Daniela F.P. Carvalho,&nbsp;Cátia F. Martins,&nbsp;Darina Kohútová,&nbsp;Obete Madacussengua,&nbsp;Mário Pinho,&nbsp;Susana P. Alves,&nbsp;Luís Ferreira,&nbsp;Margarida R.G. Maia,&nbsp;António J.M. Fonseca,&nbsp;Ana R.J. Cabrita,&nbsp;Miguel P. Mourato,&nbsp;André M. Almeida,&nbsp;Rui J.B. Bessa* and João P.B. Freire,&nbsp;","doi":"10.1021/acsagscitech.4c0074110.1021/acsagscitech.4c00741","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00741https://doi.org/10.1021/acsagscitech.4c00741","url":null,"abstract":"<p >Weaning is stressful for piglets and is often associated with decreased growth and digestive imbalances. Dietary incorporation of high-value ingredients and/or additives like microalgae and probiotics may be beneficial. To assess the combined impact of <i><i>Chlorella vulgaris</i></i> and probiotic, two 5-week growth trials were conducted, each with 60 weaning piglets, and effects on performance, digestibility, viscera morphometrics (dimensions, histology, pH, viscosity), and volatile fatty acid (VFA) profiles were evaluated. Five diets were provided <i>ad libitum</i> (<i>n</i> = 8): Control (cereal/soybean meal-based), 2.5 or 5% <i><i>C. vulgaris</i></i> inclusion, with or without commercial probiotic <i>Enterococcus lactis</i> SF68. Overall, dietary <i><i>C. vulgaris</i></i> reduced the digestibility of nutrients and energy by 1–9%, while viscera morphometrics, VFA profile, and piglet performance were largely unaffected. Probiotic supplementation kept <i><i>C. vulgaris</i></i> unaffected, suggesting no synergies at the levels used. Further studies of metabolic and gut health are needed to fully unveil the potential of <i><i>C. vulgaris</i></i> in weaning piglet diets.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 6","pages":"1014–1024 1014–1024"},"PeriodicalIF":2.3,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lignin in Fibrous Feed as an Internal Digestibility and Transit Marker in Pigs","authors":"Romy J. Veersma,&nbsp;Corentin Lannuzel,&nbsp;Walter J.J. Gerrits,&nbsp;Sonja de Vries,&nbsp;Gijs van Erven and Mirjam A. Kabel*,&nbsp;","doi":"10.1021/acsagscitech.4c0075210.1021/acsagscitech.4c00752","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00752https://doi.org/10.1021/acsagscitech.4c00752","url":null,"abstract":"<p >To assess the nutritional value of fibrous diets, external metal oxide-based markers are commonly used. Given the increasing restrictions on the use of these conventional markers, we investigated the inertness of lignin in pigs and correspondingly its potential as an internal marker. Hereto, feces and digesta were collected from pigs fed a diet containing wheat straw as sole lignin source, and lignin was quantified by ¹³C-IS py-GC-MS. Combined with detailed HSQC NMR and size-exclusion chromatography structural analysis, we revealed that lignin was recovered unmodified in feces. Comparison of lignin with TiO₂ showed similar (<i>P</i> &gt; 0.15) digestibility values for nitrogen (82.6 vs 80.2%) and fat (93.3 vs 92.5%), and a limited difference for dry matter (77.7 vs 74.1%, <i>P</i> = 0.01). Comparison of lignin with chromium-mordanted straw furthermore showed highly similar (<i>P</i> = 0.8) mean retention times of fibrous particles in the stomach. We thus confirm the potential of intrinsic lignin as a multifunctional marker for feed digestibility and digesta transit in pigs.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 6","pages":"1025–1033 1025–1033"},"PeriodicalIF":2.3,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsagscitech.4c00752","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lignin in Fibrous Feed as an Internal Digestibility and Transit Marker in Pigs.","authors":"Romy J Veersma, Corentin Lannuzel, Walter J J Gerrits, Sonja de Vries, Gijs van Erven, Mirjam A Kabel","doi":"10.1021/acsagscitech.4c00752","DOIUrl":"10.1021/acsagscitech.4c00752","url":null,"abstract":"<p><p>To assess the nutritional value of fibrous diets, external metal oxide-based markers are commonly used. Given the increasing restrictions on the use of these conventional markers, we investigated the inertness of lignin in pigs and correspondingly its potential as an internal marker. Hereto, feces and digesta were collected from pigs fed a diet containing wheat straw as sole lignin source, and lignin was quantified by ¹³C-IS py-GC-MS. Combined with detailed HSQC NMR and size-exclusion chromatography structural analysis, we revealed that lignin was recovered unmodified in feces. Comparison of lignin with TiO₂ showed similar (<i>P</i> > 0.15) digestibility values for nitrogen (82.6 vs 80.2%) and fat (93.3 vs 92.5%), and a limited difference for dry matter (77.7 vs 74.1%, <i>P</i> = 0.01). Comparison of lignin with chromium-mordanted straw furthermore showed highly similar (<i>P</i> = 0.8) mean retention times of fibrous particles in the stomach. We thus confirm the potential of intrinsic lignin as a multifunctional marker for feed digestibility and digesta transit in pigs.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 6","pages":"1025-1033"},"PeriodicalIF":2.3,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12175119/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144334622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Iron Sulfide-Impregnated Sepiolite Nanocomposite (FeS-Sp NC) Induced Differential Responses in Physiology, Iron Modulation, Biochemical Assays, and Antioxidative Defense in Direct-Seeded Rice","authors":"Khushboo Rathour,&nbsp;Anjali Sidhu*,&nbsp;Vivek Sharma and Anu Kalia,&nbsp;","doi":"10.1021/acsagscitech.4c0033510.1021/acsagscitech.4c00335","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00335https://doi.org/10.1021/acsagscitech.4c00335","url":null,"abstract":"<p >Direct-seeded rice (DSR) faces low iron bioavailability due to the plurality in the oxidation states of iron. Rapid ferrous oxidation in low-irrigation soils poses a challenge to the optimum supply of iron for the initial growth in rice. Using advancements in nanotechnology, a new iron formulation, iron sulfide-impregnated sepiolite nanocomposite (FeS-Sp NC), was prepared by an <i>in situ</i> combination of ferrous and sulfide ions on a sepiolite matrix under ultrasonic irradiation. Nanopriming of rice seeds (PR 126) with FeS-Sp NC at an optimized dose (500 μg/g) for 12 h revealed a notable enhancement in seedling growth metrics, namely, germination percentage (15.39%), shoot length (40.54%), root length (21.67%), seedling fresh weight (15.57%), dry weight (11.36%), and vigor index (28.50%) with augmented iron content, outperforming traditionally used FeSO₄ (at an optimized dose of 750 μg/g). Enhanced activity of defensive enzymes (in shoot, root), viz. SOD (13.81%; 8.78%), APX (16.66%; 3.31%), CAT (10.84%; 13.83%), and PPO (150.41%, 191.30%) indicated a stronger defense mechanism. The elevated levels of total phenolics (49.81%; 28.79% in shoot and root respectively), flavonoids (21.75% in roots), DPPH (37.15% in roots), and ABTS (13.79% in roots) as compared to FeSO₄ further rationale the invigoration of the rice seeds. Overall, FeS-Sp NC-infused nanopriming presented superior invigoration potential over FeSO₄ mediated by an antioxidative defense mechanism due to enhanced iron assimilation in seedlings, offering a promising solution for iron modulation under sustainable agricultural practices.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 6","pages":"949–961 949–961"},"PeriodicalIF":2.3,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in the Proteome of Japanese Quails’ Muscles Fed with the Supplements of Phytases of Fungal and Bacterial Origin","authors":"Leonid I. Kovalev,&nbsp;Marina A. Kovaleva,&nbsp;Ekaterina A. Ovseychik,&nbsp;Olga I. Klein,&nbsp;Diana A. Koshelkova,&nbsp;Natalia N. Gessler,&nbsp;Maria A. Danilova,&nbsp;Yulia I. Deryabina and Elena P. Isakova*,&nbsp;","doi":"10.1021/acsagscitech.4c0055410.1021/acsagscitech.4c00554","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00554https://doi.org/10.1021/acsagscitech.4c00554","url":null,"abstract":"<p >The changes in the proteomic profiles of pectoral and thigh muscles of Japanese quails (<i>Coturnix japonica</i>) were studied when a biological supplement based on the phytase from <i>Obesumbacterium proteus</i>, encapsulated in <i>Yarrowia lipolytica</i> yeast, was introduced into the diet compared to the commercial fungal phytase (Ladozym proxy) from <i>Aspergillus ficuum</i>. Both phytase preparations provided some changes in the histologic pattern of muscles and increased the protein amount. However, the encapsulated phytase from <i>O. proteus</i> acted more efficiently at normal levels of available phosphorus than the fungal phytase from <i>A. ficuum</i>. It provided a concomitant increase in troponin family proteins and a significant change in the contractility of skeletal muscles. Also, for all the groups using the phytases in the diet, we first demonstrated the acetylation of the N-terminal part of the molecule for four quails’ proteins, namely, parvalbumin alpha isoform X2 (<i>PVALB</i>), <span>l</span>-lactate dehydrogenase A chain (<i>LDHA</i>), a fragment of PDZ, and LIM domain protein 5 isoform X9 (<i>LOC107313615</i>) that could be related to the changes in forming the contractile apparatus of the muscles and the redistribution of the metabolites between aerobic and anaerobic metabolic pathways. It could impact the meat quality via acetylation/deacetylation of some enzymes related to glycogenolysis/glycolysis.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 6","pages":"962–980 962–980"},"PeriodicalIF":2.3,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}