ACS agricultural science & technology最新文献

Beyond Boundaries: Art, Science, and the Power of Representation 超越边界：艺术、科学和表现的力量

IF 2.3

ACS agricultural science & technology Pub Date : 2025-03-17 DOI: 10.1021/acsagscitech.5c0007810.1021/acsagscitech.5c00078

Emily A. Dustman*,

引用次数: 0

Preparation of a Sericin Composite Film Loaded with Rosmarinic Acid-Allicin Antibacterial Nanoparticles for Active Food Packaging Application 为活性食品包装应用制备负载香豆素-大蒜素抗菌纳米颗粒的丝胶复合膜

IF 2.3

ACS agricultural science & technology Pub Date : 2025-03-12 DOI: 10.1021/acsagscitech.4c0078310.1021/acsagscitech.4c00783

Bihua Xia, Mingxuan Hou, Runyi Qu, Yang Wang, Ting Li, Mingqing Chen, Shibo Wang and Weifu Dong*,

{"title":"Preparation of a Sericin Composite Film Loaded with Rosmarinic Acid-Allicin Antibacterial Nanoparticles for Active Food Packaging Application","authors":"Bihua Xia, Mingxuan Hou, Runyi Qu, Yang Wang, Ting Li, Mingqing Chen, Shibo Wang and Weifu Dong*, ","doi":"10.1021/acsagscitech.4c0078310.1021/acsagscitech.4c00783","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00783https://doi.org/10.1021/acsagscitech.4c00783","url":null,"abstract":"This paper fabricated a new type of sericin composite film loaded with rosmarinic acid-allicin nanoparticles by the precipitation polymerization method and casting film method. Rosmarinic acid-allicin nanoparticles were used as antibacterial agents, and sericin was used as the carrier material of rosmarinic acid-allicin nanoparticles. The structures of rosmarinic acid-allicin nanoparticles and rosmarinic acid-allicin nanoparticle-sericin composite films were characterized by a Fourier transform infrared spectrometer, a UV–visible spectrophotometer, and a scanning electron microscope. The mechanical strength (0.56 MPa tensile strength and 152.1% strain rate), UV shielding performance (>80%), antioxidant performance (>90%), antibacterial performance (>99%), and biocompatibility (>85%) of rosmarinic acid-allicin nanoparticle-sericin composite films were researched. Test results demonstrated that this rosmarinic acid-allicin nanoparticle-sericin composite film exhibited high UV shielding performance, high antioxidant performance, high biocompatibility, and high antibacterial performance. In addition, this rosmarinic acid-allicin nanoparticle-sericin composite film can delay food spoilage as a packaging material effectively. This rosmarinic acid-allicin nanoparticle-sericin composite film can be used in the field of food packaging materials.","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 4","pages":"640–648 640–648"},"PeriodicalIF":2.3,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phycobiostimulants: Next-Generation Sustainable Agricultural Inputs 植物生物刺激素：下一代可持续农业投入

IF 2.3

ACS agricultural science & technology Pub Date : 2025-03-11 DOI: 10.1021/acsagscitech.4c0077710.1021/acsagscitech.4c00777

Pushp Sheel Shukla*, Izabela Michalak*, Anne-Sophie Burlot, Nathalie Bourgougnon, Bruno Daridon, Domenico Ronga, Anicia Hurtado and Alan T. Critchley,

{"title":"Phycobiostimulants: Next-Generation Sustainable Agricultural Inputs","authors":"Pushp Sheel Shukla*, Izabela Michalak*, Anne-Sophie Burlot, Nathalie Bourgougnon, Bruno Daridon, Domenico Ronga, Anicia Hurtado and Alan T. Critchley, ","doi":"10.1021/acsagscitech.4c0077710.1021/acsagscitech.4c00777","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00777https://doi.org/10.1021/acsagscitech.4c00777","url":null,"abstract":"Plant biostimulants are a novel class of naturally derived agricultural inputs. The grouping of categories within the regulatory term “biostimulant” contains disparate inputs, such as humates, amino acids, bacteria, and algal extracts (i.e., many extraction processes applied to a relatively small group of selected seaweeds/microalgae). All seem to have the ability to improve the health and abiotic stress resistance of the treated plants. Even extracts of seaweeds (phycobiostimulants) are considerably different from one another (they are not all the same yet commonly referred to as seaweed extract, even in peer-reviewed publications), and current commercial offerings are available from a select group of green, red, and brown seaweeds. Commercial formulations combine different seaweed extracts and increasingly leverage synergies by blending biostimulants with other agricultural inputs and agrochemicals. Extensive research highlights that various algal-based extracts can effectively support both abiotic and biotic stress resistance when applied to both treated terrestrial plants and other cultivated algae. This article explores the scientific nuances of phycobiostimulants, challenging traditional regulatory classifications and emphasizing their essential role in sustainable agronomy, growing phyconomy, and global food security.","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 4","pages":"424–453 424–453"},"PeriodicalIF":2.3,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological and Metabolic Responses of Wheat (Triticum aestivum L.) after One-Generation Exposure to Perfluorooctanesulfonic Acid (PFOS) 小麦（Triticum aestivum L.）一代暴露于全氟辛烷磺酸（PFOS）后的生理和代谢反应

IF 2.3

ACS agricultural science & technology Pub Date : 2025-03-07 DOI: 10.1021/acsagscitech.4c0072210.1021/acsagscitech.4c00722

Olamide R. Ogundele, Mary Fakunle, Riley Pope-Buss, Jacob Churchman, Blessing Akinwande, Naum Kirwa, Polycarp C. Ofoegbu and Cyren M. Rico*,

{"title":"Physiological and Metabolic Responses of Wheat (Triticum aestivum L.) after One-Generation Exposure to Perfluorooctanesulfonic Acid (PFOS)","authors":"Olamide R. Ogundele, Mary Fakunle, Riley Pope-Buss, Jacob Churchman, Blessing Akinwande, Naum Kirwa, Polycarp C. Ofoegbu and Cyren M. Rico*, ","doi":"10.1021/acsagscitech.4c0072210.1021/acsagscitech.4c00722","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00722https://doi.org/10.1021/acsagscitech.4c00722","url":null,"abstract":"The pattern of plant responses, particularly on the seeds/grains metabolite profile, after generational exposure to contaminants is not well documented. Seeds from wheat cultivated in soil amended with PFOS at 0 and 25 mg/kg in the first generation were grown in clean soil to produce daughter plants and seeds in the second generation and assigned treatment combinations of 0–0 mg/kg PFOS and 25–0 mg/kg PFOS. Plant stress and responses including growth and biomass production, chlorophyll content, lipid peroxidation, and enzyme activity were measured over a short exposure period (21 days growth period). Biomass yields, elemental concentration, and grain metabolites were also measured after a long exposure period (92 days growth period). The daughter plants exhibited decreased chlorophyll content and lipid peroxidation in a short exposure period. The elemental concentrations were mostly not affected except for changes in microelements, except B, in the grains. In the metabolomics analysis, grains harvested from plants previously exposed to PFOS (i.e., 25–0 mg/kg PFOS) showed increased abundances of sucrose, linolenic acid, tryptophan, inositol-4-monophosphate, and ferulic acid, perhaps in response to adaptation to former stress. The current findings seem to suggest that one-generation exposure to PFOS does not cause detrimental effects on the next generation after the cessation of exposure. The results provide insights into the effects of generational exposure of plants to PFOS.","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 4","pages":"593–602 593–602"},"PeriodicalIF":2.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsagscitech.4c00722","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Asymmetric Wetting Janus Mesoporous Silica Nanopesticide Delivery System with Enhanced Foliar Deposition Ability and Sustained-Release Behavior for Increasing Pesticide Utilization Efficiency 具有增强叶面沉积能力和缓释特性的非对称湿式介孔二氧化硅纳米释药系统提高农药利用效率

IF 2.3

ACS agricultural science & technology Pub Date : 2025-03-07 DOI: 10.1021/acsagscitech.4c0067010.1021/acsagscitech.4c00670

Jihao Zuo*, Yitong Lin, Jinting Cai, Ruopeng Lan, Dongyan Yang, Li Hao, Jingli Cheng, Hongjun Zhou and Xinhua Zhou*,

{"title":"Asymmetric Wetting Janus Mesoporous Silica Nanopesticide Delivery System with Enhanced Foliar Deposition Ability and Sustained-Release Behavior for Increasing Pesticide Utilization Efficiency","authors":"Jihao Zuo*, Yitong Lin, Jinting Cai, Ruopeng Lan, Dongyan Yang, Li Hao, Jingli Cheng, Hongjun Zhou and Xinhua Zhou*, ","doi":"10.1021/acsagscitech.4c0067010.1021/acsagscitech.4c00670","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00670https://doi.org/10.1021/acsagscitech.4c00670","url":null,"abstract":"Mitigating inefficiencies and environmental pollution caused by weak foliar adhesion and rapid release during pesticide application is a significant challenge in agricultural development. This study addresses this issue by preparing asymmetric wetting Janus mesoporous silica nanocarriers (AWJ-MSN) through the adjustment of hydrophilic and hydrophobic monomer ratios via interfacial modification. Subsequently, asymmetric wetting Janus nanopesticide delivery systems (Thi@AWJ-MSN) were constructed by encapsulating the model pesticide molecule, thiafuramide (Thi). The coexistence of hydrophilic and hydrophobic segments on the AWJ-MSN imparts interfacial activity, enhancing spreadability on plant leaves with different wettability (Brassica chinensis L., Cucumis sativus L., and Arachis hypogaea L.). The droplet impact behavior of the AWJ-MSN on plant leaves and artificial PTFE membrane surfaces was analyzed through normalization, revealing a reduced normalized spreading height (Ht/D0) and an increased normalized spreading diameter (Dt/D0). These findings indicate the enhanced foliar deposition ability of the AWJ-MSN under real-world foliar spraying conditions. Furthermore, the encapsulation of Thi within the nanocarriers protects the active pesticide ingredients, enabling sustained release through quasi-Fickian diffusion and ultimately prolonging the pesticide’s efficacy. This study provides theoretical guidance for the aqueous formulation and reduction of pesticide use, contributing to reduced environmental risks and offering potential applications in agriculture.","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 4","pages":"569–582 569–582"},"PeriodicalIF":2.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Lipidomics and Growth Response of Flammulina velutipes Mycelium under Blue and White LED Light Spectra and Various Intensities 蓝白光和不同强度LED下金针菇菌丝体脂质组学比较及生长响应

IF 2.3

ACS agricultural science & technology Pub Date : 2025-03-06 DOI: 10.1021/acsagscitech.4c0054110.1021/acsagscitech.4c00541

Jiaxin Fan, Dongmei Wang, Shuhei Kaneko and Kuniyoshi Shimizu*,

{"title":"Comparative Lipidomics and Growth Response of Flammulina velutipes Mycelium under Blue and White LED Light Spectra and Various Intensities","authors":"Jiaxin Fan, Dongmei Wang, Shuhei Kaneko and Kuniyoshi Shimizu*, ","doi":"10.1021/acsagscitech.4c0054110.1021/acsagscitech.4c00541","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00541https://doi.org/10.1021/acsagscitech.4c00541","url":null,"abstract":"Mushroom mycelia, such as Flammulina velutipes (enokitake), have significant potential as food resources and models for fungal biology. This study investigated the effects of blue and white LED lights, each at varying intensities (5, 10, 15, and 20 μmol·m–2·s–1), on the biomass production, growth rate, and lipidome composition of two F. velutipes strains. Results indicated that blue light significantly enhanced biomass production and growth rate, especially at 10 μmol·m–2·s–1, increasing biomass by 74.60% and 52.96% for the two strains compared to white light. Lipidomics revealed PE 18:2_20:3, PC 18:2_19:2, and Cer t19:1_16:0 as core lipids responsive to blue light, while PC 18:2_18:3 and PE 18:2_18:3 increased under white light. Pathway analysis highlighted the upregulation of glycerophospholipid, arachidonic acid, and sphingolipid metabolism in different light spectra. These findings underscore the role of light spectra in the regulation of mycelial growth and lipid biosynthesis for controlled environment agriculture.","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 4","pages":"487–498 487–498"},"PeriodicalIF":2.3,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Measurement of Bystander Dermal Exposure Resulting from Drift after Drone Application under Three Crop Treatment Scenarios 测量三种作物处理方案下无人机施药后漂移导致的旁观者皮肤接触情况

IF 2.3

ACS agricultural science & technology Pub Date : 2025-03-04 DOI: 10.1021/acsagscitech.4c0064910.1021/acsagscitech.4c00649

Beibei Gao, Juan Sasturain, Christiane Wiemann*, Nicola J. Hewitt, Wei Jia Gan, Guobin Wang, Yubin Lan and Udo Blaschke,

{"title":"Measurement of Bystander Dermal Exposure Resulting from Drift after Drone Application under Three Crop Treatment Scenarios","authors":"Beibei Gao, Juan Sasturain, Christiane Wiemann*, Nicola J. Hewitt, Wei Jia Gan, Guobin Wang, Yubin Lan and Udo Blaschke, ","doi":"10.1021/acsagscitech.4c0064910.1021/acsagscitech.4c00649","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00649https://doi.org/10.1021/acsagscitech.4c00649","url":null,"abstract":"The use of drones for plant protection product (PPP) applications is increasing; however, field data are lacking for drift exposure to bystanders and residents, allowing one to compare different exposure scenarios. We determined the dermal exposure of bystanders from spray drift after drone application to bare soil, soybean crops, and peach tree orchards. Dermal exposure was increased in orchard crops at higher wind speeds and lower when mannequins were placed further away from the application site. Approximately 90% of the total spray drift was deposited on mannequins within 5 m from the field edge. Drift exposure after drone spraying was lower than predicted values for ground boom applications compared to predictions using knapsacks. In conclusion, our study confirms the operator and bystander exposure-related benefits of the use of drones for applying PPPs to field and orchard crops.","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 4","pages":"542–551 542–551"},"PeriodicalIF":2.3,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsagscitech.4c00649","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and Validation of an Analytical Methodology To Determine Deltamethrin Residues and Its Metabolites in Pine Products Using GC-QTOF-MS GC-QTOF-MS测定松木制品中溴氰菊酯残留及其代谢物分析方法的建立与验证

IF 2.3

ACS agricultural science & technology Pub Date : 2025-03-04 DOI: 10.1021/acsagscitech.5c0004410.1021/acsagscitech.5c00044

Ana Jano, Adrián Fuente-Ballesteros, José Bernal, Ana M. Ares and Silvia Valverde*,

{"title":"Development and Validation of an Analytical Methodology To Determine Deltamethrin Residues and Its Metabolites in Pine Products Using GC-QTOF-MS","authors":"Ana Jano, Adrián Fuente-Ballesteros, José Bernal, Ana M. Ares and Silvia Valverde*, ","doi":"10.1021/acsagscitech.5c0004410.1021/acsagscitech.5c00044","DOIUrl":"https://doi.org/10.1021/acsagscitech.5c00044https://doi.org/10.1021/acsagscitech.5c00044","url":null,"abstract":"A new analytical methodology has been proposed to determine deltamethrin and two of its main metabolites (3-phenoxybenzoic acid and 3-(2,2-dibromoethenyl)-2,2-dimethylcyclopropanecarboxylic acid) in pine needle and pine nut samples, using gas chromatography coupled to quadrupole time-of-flight mass spectrometry. Two sample treatments were developed consisting of a solvent extraction for pine needles and a modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) methodology for pine nuts, both followed by an additional cleanup step. The eco-friendliness and practicality of the methods were assessed using modified green analytical procedure index and blue applicability grade index, respectively. The method was validated in terms of selectivity, limits of detection (0.2–26 μg kg–1) and quantification (0.6–79 μg kg–1), linearity, matrix effect (< ±20%), trueness (recoveries 82–102%), and precision (relative standard deviation <9%). An analysis of 20 Spanish samples revealed that no residues were found in any of them.","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 4","pages":"656–666 656–666"},"PeriodicalIF":2.3,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sulfate Additives Cut Methane Emissions More Effectively at Lower Liquid Manure Storage Temperatures 硫酸盐添加剂在较低的液态粪便储存温度下更有效地减少甲烷排放

IF 2.3

ACS agricultural science & technology Pub Date : 2025-03-04 DOI: 10.1021/acsagscitech.4c0065910.1021/acsagscitech.4c00659

Filipe Matos Pereira Lima, Mélodie Laniel, Hambaliou Baldé, Robert Gordon and Andrew VanderZaag*,

{"title":"Sulfate Additives Cut Methane Emissions More Effectively at Lower Liquid Manure Storage Temperatures","authors":"Filipe Matos Pereira Lima, Mélodie Laniel, Hambaliou Baldé, Robert Gordon and Andrew VanderZaag*, ","doi":"10.1021/acsagscitech.4c0065910.1021/acsagscitech.4c00659","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00659https://doi.org/10.1021/acsagscitech.4c00659","url":null,"abstract":"Reducing methane (CH4) emissions from dairy farms is a key objective in limiting total greenhouse gas emissions from the livestock industry. Reducing CH4 emissions from manure storage using additives may provide an achievable near-term contribution to this long-term goal in alignment with the International Dairy Federation’s initiative on pathways to net zero. Sulfate-based H2SO4 and the sulfate-containing nonacid CaSO4 have effectively suppressed methane emissions in lab studies at a single temperature. The present study analyzes the effect of temperature on the efficacy of these two additives, bridging the gap between common laboratory conditions and average on-farm temperature. We found superior cumulative suppression, higher peak suppression, and longer duration of high-end suppression at lower temperatures when comparing controls to additive experiments at 24, 21, and 18 °C over 120 days. Peak mitigation increased as temperature decreased, culminating at 82.9% and 57.6% for H2SO4 and CaSO4, respectively, at 18 °C. Additives remained effective for longer at lower temperatures, with H2SO4 maintaining ≥70% peak mitigation (PM) for 102 days at 18 °C, but only 48 days at 24 °C; CaSO4 retained ≥70% PM for 87 days at 18 °C, but only 38 days at 24 °C. PM for each additive occurs at similar thermal times, despite appearing different at conventional times. Our analysis creates a link between the efficacy of CH4 mitigation and local temperatures, which can be related to cumulative heat (thermal time/degree-days) to establish site-specific guidance for CH4 mitigation protocols.","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 4","pages":"552–558 552–558"},"PeriodicalIF":2.3,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsagscitech.4c00659","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic Engineering of Saccharomyces cerevisiae for High Bioproduction of (+)-Nootkatone 酿酒酵母高产(+)-诺卡酮的代谢工程研究

IF 2.3

ACS agricultural science & technology Pub Date : 2025-02-28 DOI: 10.1021/acsagscitech.4c0073110.1021/acsagscitech.4c00731

Ruying Gong, Qihang Chen, Wenqian Wei, Hanning Deng, Weizhu Zeng, Song Gao, Jingwen Zhou and Sha Xu*,

{"title":"Metabolic Engineering of Saccharomyces cerevisiae for High Bioproduction of (+)-Nootkatone","authors":"Ruying Gong, Qihang Chen, Wenqian Wei, Hanning Deng, Weizhu Zeng, Song Gao, Jingwen Zhou and Sha Xu*, ","doi":"10.1021/acsagscitech.4c0073110.1021/acsagscitech.4c00731","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00731https://doi.org/10.1021/acsagscitech.4c00731","url":null,"abstract":"(+)-Nootkatone, a high-value sesquiterpenoid compound, has found great applications in food, agriculture, and pharmaceutical fields. The biosynthesis of (+)-nootkatone is considered as a green and sustainable synthetic method but is mainly challenged by the significant accumulation of the precursor (+)-valencene during the fermentation process. In the present study, the genes CnVS, HPOV480A/V482A, AtCPR1, and ZSD1 were integrated into the genome of strain SQ1 and the original promoter of ERG9 was replaced by PHXT1 for constructing the (+)-nootkatone biosynthetic pathway and inhibiting the competitive ergosterol synthesis pathway, respectively. Subsequently, the flexible linkers and promoters of ERG20 and CnVS were screened and optimized to reduce the shuttling distance of the substrate between the enzymes. Further, ERG20-(GSG)2-CnVS, HPOV480A/V482A, AtCPR1, and ZSD1 were overexpressed, resulting in a titer of 244.1 mg/L (+)-nootkatone. The signal peptide of HPOV480A/V482A was rationally engineered to improve the titer of (+)-nootkatone to 466.1 mg/L, while the titer of valencene was reduced to 29.3 mg/L. Molecular dynamics simulations showed more stable binding between HPOV480A/V482A and the substrate after modifying the signal peptide and thus enhanced enzyme activity. Finally, the maximum (+)-nootkatone titer of 6.5 g/L was obtained in a 5 L bioreactor. This is the highest (+)-nootkatone titer reported to date. Thus, the present study provides a solid foundation for the future large-scale production of (+)-nootkatone.","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 4","pages":"583–592 583–592"},"PeriodicalIF":2.3,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0