ACS agricultural science & technology最新文献

{"title":"Effects of Organo-Mineral Enriched Biochar Fertilizers on Physicochemical Characteristics and Bioactive Potential of Ginger (Zingiber officinale)","authors":"Maddage Ayesha Nimanthi Jayathilaka,&nbsp;Thanuja Janadari Dhanapala,&nbsp;Anushka Upamali Rajapaksha,&nbsp;Meththika Vithanage,&nbsp;Paramullage Upamali Sandaruwan Peiris,&nbsp;Garusinghe Devage Malinda Prabhath Madhusankha,&nbsp;Chamila Vinodanee Liyanage Jayasinghe","doi":"10.1021/acsagscitech.6c00572","DOIUrl":"https://doi.org/10.1021/acsagscitech.6c00572","url":null,"abstract":"<p>Ginger (<em>Zingiber officinale</em>) is a globally consumed spice valued for its distinctive flavor and therapeutic properties, delivered by different bioactive constituents, including 6-gingerol. With increasing demand for functional foods and stringent international quality standards, producing ginger with consistent physicochemical quality and enhanced bioactive composition is a key challenge. Cultivation practices, especially growth media, play a decisive role in determining rhizome quality; however, integrated evaluations linking growth media to both compositional and functional attributes are limited. This study investigated the influence of different growth media on ginger quality and export suitability. Ginger was cultivated in compost and in two organo-mineral-enriched biochar fertilizer-based growth media (fish-based and green-based) at application rates of 1, 2.5, and 5 t ha^–1. Growth media significantly influenced the compositional and functional attributes of ginger. Green-based enriched media at 5 t ha^–1 produced the highest total phenolic (41.48 mg GAE g^–1) and flavonoid contents (9.69 mg RE g^–1), while fish-based enriched media at the same rate yielded the greatest antioxidant capacity (47.92 mmol Fe²⁺ g^–1). The highest oleoresin content was reported in fish- and green-based-enriched media at 5 t ha^–1, and fish-based media produced the best 6-gingerol yield. Fish 5 t ha^–1 exhibited a significantly higher protein content, approximately 3-fold higher than in the control and compost treatments. Growth media strongly influence the physicochemical and bioactive properties of ginger. Fish and green-based biochar media, particularly at higher application rates, enhance functional quality and bioactive accumulation, providing a practical strategy for producing premium-grade ginger suitable for export markets.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"6 10","pages":"2167–2176"},"PeriodicalIF":3.4,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149210947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ecotoxicities and Bioactivities of Different Dinotefuran Formulations: Influencing Factors and Risk Assessment","authors":"Jiahua Li,&nbsp;Manni Wu,&nbsp;Yuxia Yang,&nbsp;Xianbin Li,&nbsp;Zenglong Chen","doi":"10.1021/acsagscitech.6c00391","DOIUrl":"https://doi.org/10.1021/acsagscitech.6c00391","url":null,"abstract":"<p>To elaborate on the differences in biological activities and ecological toxicities of multiformulations of agrichemicals with influencing factors, in terms of exposure mode, exposure time, pesticide adjuvant, and test biological species, the bioactivity of five dinotefuran formulations against three targets was studied, which was 17.3–181.2 mg/L. Dinotefuran SC was the most effective formulation for controlling <em><em>Besimia tabaci</em></em> (LC_50,17.3 mg/L), and dinotefuran WP showed the best activity against <em><em>Frankliniella occidentalis</em></em> (19.4 mg/L) and <em><em>Sitobion avenae</em></em> (35.7 mg/L). The acute toxicity of all dinotefuran formulations to <em><em>Apis mellifera</em></em> by oral assay mode was 27.4–423.4 times higher than that by contact mode. <em><em>Apis melliferas</em></em> suffered serious hazards following oral exposure to GR dinotefuran with 7.8 × 10^–2 mg/L at 48 h. <em><em>A. mellifera</em></em> suffered the highest ecotoxic risks at 7.4–1222.8 when exposed to dinotefuran WG, followed by SC formulation (RQ, 2.2–1111.1), SG (9.1–882.4), WP (10.1–646.6), and GR (1.3–173.1). The oral exposure risks of dinotefuran formulations were 558.5–1222.8 on <em><em>A. melliferas</em></em>, which were significantly greater than the contact exposure risks of 1.3–23.6. Additionally, 1.2–2.3 times RQ had been amplified for dinotefuran formulations to <em><em>A. mellifera</em></em> after exposure for 24 to 48 h. The study provided a theoretical basis for reducing application and increasing efficiency and afforded a scientific reference for the development of green pesticide formulations.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"6 10","pages":"2114–2121"},"PeriodicalIF":3.4,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149210961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liquid Fermentation, Formulation, and Bioefficacy of Metarhizium rileyi Microsclerotia for Control of Spodoptera frugiperda","authors":"Isabela Fetter,&nbsp;Camila C. Moreira,&nbsp;Gabriel M. Mascarin,&nbsp;Janaina B. Seibert,&nbsp;Italo D. Júnior","doi":"10.1021/acsagscitech.6c00406","DOIUrl":"https://doi.org/10.1021/acsagscitech.6c00406","url":null,"abstract":"<p>Microsclerotia (MS) are melanized hyphal aggregates produced by submerged liquid fermentation by entomopathogenic fungi such as <em>Metarhizium rileyi</em>, an important pathogen of lepidopteran pests including the fall armyworm, <em>Spodoptera frugiperda</em>. In this study, we evaluated MS production under different carbon-to-nitrogen (C:N) ratios and assessed the bioefficacy of MS against <em>S. frugiperda</em>. Lower C:N ratios (10:1 and 12:1) yielded 1.4 × 10⁴ and 3.4 × 10⁴ MS/mL within five days of fermentation, respectively. MS also germinated and sporulated more rapidly on maize and soybean leaves than on water agar, reaching 94.9% and 92.7% germination, respectively, after 24 h, compared with 29.3% on the artificial substrate. A granular formulation containing 1 × 10⁵ MS/g + diatomaceous earth caused 95% mortality of 3rd instar <em>S. frugiperda</em> (but only 2.5% sporulation) on leaves and 85.5% (with 38.8% sporulation) of prepupal mortality in soil. In addition, MS granules maintained high viability after six months of storage under refrigerated conditions. These results demonstrate that <em>M. rileyi</em> MS granules are stable during cold storage and support high germination and sporulation upon high humidity. Hence, <em>M. rileyi</em> MS + diatomaceous earth granules could potentially offer an additional strategy based on inoculative control to the toolbox management of <em>S. frugiperda</em>.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"6 10","pages":"2134–2143"},"PeriodicalIF":3.4,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149210944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol and Plant Growth-Promoting Potential of Volatile Organic Compound-Producing Endophytic Bacillus safensis and Serratia liquefaciens against Sclerotinia sclerotiorum in Helianthus annuus L","authors":"Rajwinder Kaur,&nbsp;Pratibha Vyas,&nbsp;Abdullah S. Alsohim","doi":"10.1021/acsagscitech.6c00602","DOIUrl":"https://doi.org/10.1021/acsagscitech.6c00602","url":null,"abstract":"<p>The present study is aimed at evaluating biocontrol and growth-promoting potential of volatile organic compound (VOC) producing bacterial endophytes against <em><em>Sclerotinia sclerotiorum</em></em> in <em><em>Helianthus annuus</em></em> L. Two isolates SEB₆ and SEB₂₃, exhibiting fungal growth inhibition of 23.75 and 20.0%, respectively, in a dual-plate assay and 76.5 and 35.4%, respectively, in broth were identified by morphological features, biochemical tests, matrix-assisted laser desorption/ionization time of flight mass spectroscopy and 16S rRNA gene sequencing as <em><em>Bacillus safensis</em></em> and <em><em>Serratia liquefaciens</em>.</em> Scanning electron microscopy revealed that fungal growth was inhibited by bacterial antagonists by rupturing, breaking, and shrinking of fungal hyphae. Additionally, the antagonists exhibited phosphate solubilization, and auxins, ammonia, and lipase production. Both strains produced various volatile organic compounds as detected by gas chromatography–mass spectrometry, including 26 VOCs in <em><em>Bacillus safensis</em></em> SEB₆ and 16 in <em><em>Serratia liquefaciens</em></em> SEB₂₃. The compounds 1-docosene, isopropyl myristate, 1,2-benzenedicarboxylic acid, octadecene, diethyl phthalate, glycerin, triacetin, and tetradecane were the common VOCs produced by both the strains. Colonization of plant tissues, as evident by scanning electron microscopy analysis, validated the endophytic nature of both the strains. The pot trial revealed that the combined application of both the strains decreased the disease severity by 88.9% and significantly improved the vigor index, plant growth, total phenol content, and chlorophyll content over control treatment. The maximum increase in N, P, and K contents of 1.73, 0.54, and 6.8%, respectively, was obtained with the treatment <em>Bs</em>SEB₆ + <em>Sl</em>SEB₂₃ in the presence of the fungal pathogen. To the best of our knowledge, this paper for the first time reports the production of 3-pentadecylphenol by the antagonistic <em><em>Bacillus safensis</em></em> strain.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"6 10","pages":"2177–2189"},"PeriodicalIF":3.4,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149210924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tolerance of Transgenic Soybean Cultivars to the Herbicide Picloram","authors":"Maria Cecília Costa Godeiro,&nbsp;Arthur Allan Sena de Oliveira,&nbsp;Fernanda da Silva Soares,&nbsp;Francisca Daniele da Silva,&nbsp;María Carolina Ramírez Hernández,&nbsp;Paulo Sérgio Fernandes das Chagas,&nbsp;João Everthon da Silva Ribeiro,&nbsp;Luiz Fernando de Sousa Antunes,&nbsp;Guilherme Braga Pereira Braz,&nbsp;Daniel Valadão Silva","doi":"10.1021/acsagscitech.6c00392","DOIUrl":"https://doi.org/10.1021/acsagscitech.6c00392","url":null,"abstract":"<p>The cultivation of transgenic soybean has expanded in Brazil, often in areas previously used for pasture, where auxin herbicides, such as picloram, are widely used. This herbicide is highly persistent in the soil, which can compromise subsequent sensitive crops, such as soybean. Thus, the present study evaluated the tolerance of transgenic soybean cultivars to picloram. Two experiments were conducted in a randomized complete block design, with four replicates, in a 2 × 10 factorial scheme, involving two cultivars (Olimpo IPRO and Tormenta CE) and 10 doses of picloram, ranging from 0 to 4.80 g a.i. ha^–1. The evaluations included visual symptoms of injury (7 to 56 days after emergence-DAE), leaf area, and dry matter accumulation (leaves, stems, pods, roots, and total) at 30 and 60 DAE. The quantification of picloram residues in the soil was performed by UHPLC in the same periods. Both cultivars showed progressive symptoms of poisoning with increasing dose and exposure time to the compound, resulting in significant reductions in leaf area and dry matter. Detectable picloram residues remained in the soil up to 60 DAE at the highest doses applied (4.80 g a.i. ha^–1). Although the Tormenta CE cultivar is tolerant to auxinic herbicides, it did not show tolerance to picloram, despite its belonging to this mode of action. These results highlight the importance of choosing the right cultivar in areas with a history of picloram application, to reduce the risks of injury and minimize adverse effects on soybean growth and reproductive development.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"6 10","pages":"2122–2133"},"PeriodicalIF":3.4,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149210937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromones: Important Templates for Pesticide Development","authors":"Parshant Kaushik,&nbsp;Riya Kundu,&nbsp;Najam Akhtar Shakil,&nbsp;Virendra Singh Rana,&nbsp;Rajesh Kumar,&nbsp;Neethu Narayanan,&nbsp;Kailashpati Tripathi,&nbsp;Rakesh Kumar","doi":"10.1021/acsagscitech.6c00345","DOIUrl":"https://doi.org/10.1021/acsagscitech.6c00345","url":null,"abstract":"<p>The chromone moiety is a fundamental component of various plant pigments, including flavones, xanthones, anthocyanins, and anthocyanidins, which are well-known for their distinctive colors and play a significant role in plants by creating visually appealing patterns that attract pollinators. Furthermore, chromones contribute to the plant's defense mechanisms by providing protection against UV radiation and fungal infections. Chromones offer excellent templates for structural modifications, enabling the synthesis of a wide range of compounds with diverse pharmacological properties. This feature makes chromones highly valuable in the field of medicinal chemistry. Their structural characteristics, combined with their ease of synthesis and versatility, position them as favored structures for drug development. The accessibility and structural diversity of chromones contribute to their significance in the development of new pharmaceutical compounds. Numerous scientific studies have demonstrated that synthesized chromones exhibit a diverse array of biological effects. These effects encompass various properties such as antifungal, antibacterial, antiviral, immunostimulatory, insecticidal, anti-malarial, nematicidal, anti-inflammatory, anti-tumor, and antioxidant activities. Extensive research has been carried out on chromone and its derivatives, which has proved the pharmacological importance of this heterocyclic nucleus. The present review focuses on the synthetic chemistry and pesticidal activities of chromone derivatives in the current literature with an update of recent research findings on this nucleus and the perspectives that they hold for future research.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"6 10","pages":"2017–2047"},"PeriodicalIF":3.4,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149210948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Operationally Extractable Copper and Early-Stage Phytotoxicity in Vineyard Soils: Implications for Cu Risk Assessment and Soil Management","authors":"Raquel Vázquez-Blanco,&nbsp;Rocío González-Feijoo,&nbsp;Silvia Romeo-Río,&nbsp;David Fernández-Calviño,&nbsp;Manuel Conde-Cid,&nbsp;Daniel Arenas-Lago","doi":"10.1021/acsagscitech.6c00207","DOIUrl":"https://doi.org/10.1021/acsagscitech.6c00207","url":null,"abstract":"<p>Long-term use of Cu-based fungicides in humid vineyards promotes Cu accumulation in topsoils, but plant risk depends on soil properties and on the Cu pools that are operationally extractable rather than on total Cu alone. In this study, soils were collected from vine rows and inter-row areas in 58 vineyards in Galicia, NW Spain. Soil physicochemical properties and total Cu were determined, and six chemically defined Cu fractions were assessed using deionized water, CaCl₂, NH₄OAc, NH₄Cl, EDTA, and a rhizosphere-simulating organic acid extractant. Early-stage phytotoxicity was evaluated using standardized germination and seedling growth bioassays with <em>Sinapis alba</em>, <em>Lepidium sativum</em>, and <em>Sorghum saccharatum</em>. Total Cu concentrations indicated moderate enrichment typical of long-term vineyard soils affected by Cu fungicide inputs, with a mean of 109 mg kg^–1 and a range of 16.4–301 mg kg^–1. Water and CaCl₂ extracted only approximately 1–2% of total Cu, EDTA extracted approximately 50%, and NH₄OAc, NH₄Cl, and the rhizosphere method extracted approximately 5–7%. Despite Cu enrichment, all species showed high germination rates under the short-term assay conditions. Early seedling responses were more associated with soil pH, organic matter (OM), texture, and selected extractable Cu fractions than with total Cu alone. <em>S. alba</em> showed predominantly stimulatory early growth responses, suggesting that this species may be a promising candidate for further evaluation as a cover crop in Cu-enriched vineyard soils. These results support the combined use of chemical extractions and early-stage plant bioassays for Cu risk assessment, although longer-term studies are required to evaluate chronic phytotoxicity and agronomic performance.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"6 10","pages":"2078–2088"},"PeriodicalIF":3.4,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149211005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metal‑Doped ZIF‑8 Nanoparticles Coated with Hyaluronic Acid and Loaded with Oregano Essential Oil for Inactivation of Listeria monocytogenes on Spinach (Spinacia oleracea)","authors":"Huy Loc Nguyen,&nbsp;Rosana G. Moreira,&nbsp;M. Elena Castell-Perez","doi":"10.1021/acsagscitech.6c00476","DOIUrl":"https://doi.org/10.1021/acsagscitech.6c00476","url":null,"abstract":"<p>This work evaluates an antimicrobial ZIF‑8−based nanocomposite developed through Ag/Cu doping, hyaluronic acid (HA) surface functionalization, and oregano essential oil (OEO) encapsulation to enhance activity against foodborne pathogens, including <em>Listeria monocytogenes</em>. The combined incorporation of metal dopants, a biopolymer coating, and a natural antimicrobial enables complementary physicochemical and antimicrobial functions within a single nanostructure. Nanoparticles were synthesized via ambient-temperature precipitation and comprehensively characterized using SEM, TEM, FTIR, XRD, zeta potential, particle size, and DSC tests. The triple-modified variant (OEO-Ag-ZIF-8-HA) demonstrated superior antibacterial efficacy, with MIC and MBC values of 125 μg/mL and 375 μg/mL, respectively, significantly lower (<em>p</em> &lt; 0.05) than undoped ZIF-8 (MIC: 2000 μg/mL, MBC: 8000 μg/mL). Cytotoxicity evaluation demonstrated favorable biocompatibility, with greater than 90% cell viability at 250 μg/mL for OEO-Ag-ZIF-8-HA. Testing on spinach leaves yielded reduced initial aerobic plate counts to 2.89 log CFU/g, sustaining &lt;4 log CFU/g over 5 days and a 3.38 log CFU/g reduction of <em>L. monocytogenes</em> within 15 min (<em>p</em> &lt; 0.05), showing greater antimicrobial performance than a standard chlorine control under the tested conditions. Treated spinach leaves retained a fresher appearance compared to controls over 5 days at 21 °C. These results demonstrate the potential of OEO‑Ag‑ZIF‑8‑HA as a biocompatible antimicrobial material for food safety applications.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"6 9","pages":"1969–1986"},"PeriodicalIF":3.4,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149010614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silencing of ShMYB58/63 and ShMYB85 in Saccharum spp. Alters Lignin Deposition and Can Improve Biomass Saccharification","authors":"Juan Pablo Portilla Llerena,&nbsp;Raquel Figueiredo,&nbsp;Igor Cesarino,&nbsp;Renato Vicentini,&nbsp;Rafael Gallinari,&nbsp;Pedro Araujo,&nbsp;José Carlos del Rio,&nbsp;Jorge Rencoret,&nbsp;Ana Gutiérrez,&nbsp;Michael dos Santos Brito,&nbsp;Julio Cesar García,&nbsp;Juliana Lischka Sampaio Mayer,&nbsp;Lais Soêmis Sisti,&nbsp;Raúl Lima Coasaca,&nbsp;Eduardo Kiyota,&nbsp;Paulo Mazzafera","doi":"10.1021/acsagscitech.5c01138","DOIUrl":"https://doi.org/10.1021/acsagscitech.5c01138","url":null,"abstract":"<p>A primary focus in reducing biomass recalcitrance in plants to produce second-generation bioethanol (E2G) has been the genetic manipulation of transcription factors (TFs) that regulate lignin content and composition. Little is known about lignin regulation in monocots, particularly in sugarcane, which is a promising feedstock for E2G production. We conducted a Bayesian network analysis that identified sugarcane orthologs <em>ShMYB85</em> and <em>ShMYB58/63</em> as potential activators of lignin biosynthesis in sugarcane. Then, we generated silenced transgenic lines for these TFs in the <em>Saccharum</em> spp. hybrid cultivar SP80-3280 to investigate their roles in lignin biosynthesis (the two Saccharum species, <em>S. officinarum</em> and <em>S. spontaneum</em>, were used only as the dataset for the network and expression analyses). Characterization of the transgenic lines revealed significant alterations in lignin content and composition. Notably, saccharification efficiency was enhanced in the <em>shmyb85</em> transgenic lines, suggesting that <em>shmyb85</em> may act as a broader activator than <em>shmyb58/63</em>. Our findings highlight the opportunities and challenges associated with redesigning the sugarcane cell wall through the genetic manipulation of myb TFs, ultimately aiming to enhance E2G production.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"6 9","pages":"1775–1794"},"PeriodicalIF":3.4,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149010629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient Galactomannan Assay for Clusterbean: Exploring Variability and Biochemicals Association in Seed Structural Components","authors":"Aman Verma,&nbsp;Saurabh Swami,&nbsp;Mukeshkumar Parmar,&nbsp;Hans Raj Mahla","doi":"10.1021/acsagscitech.6c00145","DOIUrl":"https://doi.org/10.1021/acsagscitech.6c00145","url":null,"abstract":"<p>Clusterbean (<em><em>Cyamopsis tetragonoloba</em></em> L.) is a valuable multipurpose leguminous crop, valued for galactomannan (GM; water-soluble, edible thickening agent) and germ’s protein, a vital source of commercial animal feed. In the present study, valuable seed component traits such as GM from endosperm, phenols and flavonoids from seed coat, and protein from germ were investigated in addition to the seed yield of 60 genotypes to ascertain multi-trait selection criteria for breeding purposes. An enzyme-based method with improved specificity was also standardized for the quantitative estimation of GM from the endosperm to assess the quality of the harvested produce. The addition of a wet grinding step and cellulase application significantly improved GM quantification and prevented underestimation due to the formation of gel plugs. Results showed that the highest GM and protein content was found in the CAZG-17-4-1 and CAZG-20-21 genotypes. The hierarchical clustered correlation heatmap showed a strong and positive correlation among thousand seed weight, GM, endosperm %, and GM content on a whole seed basis. PCA clustered biplot with genotype-labeled ellipses selected five of 60 genotypes (CAZG-21-24, CAZG-16-12, CAZG-20-59, CAZG-20-63, and CAZG-20-64), which possess all vital desirable traits like GM, germ’s protein, and seed coat phenols along with good seed yield per plant and therefore may be selected as multi-trait high-performance genotypes.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"6 9","pages":"1820–1829"},"PeriodicalIF":3.4,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149003720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}