ACS agricultural science & technology最新文献

{"title":"Synthesis of UV-Curable Biodegradable Polymer Membrane for Coating of Urea Fertilizer","authors":"Aylin Kaymaz,&nbsp; and ,&nbsp;Tarık Eren*,&nbsp;","doi":"10.1021/acsagscitech.4c0072410.1021/acsagscitech.4c00724","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00724https://doi.org/10.1021/acsagscitech.4c00724","url":null,"abstract":"<p >Nitrogen is particularly important for promoting vegetative growth, which is essential for high yields in many crops. Urea contains a high concentration of nitrogen, making it an efficient source of this essential nutrient for plant growth. However, urea is highly soluble in water, which can lead to nitrogen loss through leaching or volatilization. Coating urea granules helps reduce these losses by controlling the release of nitrogen into the soil, improving nitrogen use efficiency and thus enhancing the uptake of nitrogen by plants. In the present study, urea granules were coated with polylactic acid and ultraviolet (UV)-curable methacryl-functional chitosan. After UV curing of the biopolymer-coated urea, the surface morphology was analyzed by scanning electron microscopy and Fourier transform infrared spectroscopy. The rates of urea release in distilled water were measured by UV spectroscopy. UV curing has been demonstrated as an effective method for coating granules, resulting in reduced rates of urea dissolution in water.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 4","pages":"649–655 649–655"},"PeriodicalIF":2.3,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zein-Based Nanocarrier of the Insecticide Cyantraniliprole for the Control of Bemisia tabaciMEAM1 (Whitefly)","authors":"Felipe Franco Oliveira*,&nbsp;Vanessa Takeshita,&nbsp;Nubia Zuverza-Mena,&nbsp;Juliana Milagres,&nbsp;Carlos Tamez,&nbsp;Washington Luiz da Silva,&nbsp;Jhones Luiz Oliveira,&nbsp;Anderson Espirito Santo Pereira,&nbsp;Leonardo Fernandes Fraceto,&nbsp;Jorge Alberto Marques Rezende,&nbsp;Christian O. Dimkpa and Jason C. White,&nbsp;","doi":"10.1021/acsagscitech.4c0075110.1021/acsagscitech.4c00751","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00751https://doi.org/10.1021/acsagscitech.4c00751","url":null,"abstract":"<p ><i>Bemisia tabaci</i>, also known as whitefly, is a sap-sucking polyphagous insect that severely affects important crops worldwide. The growing demand for environmentally friendly and effective pest control measures necessitates new approaches to reduce the volume and frequency of insecticide application to crops while maintaining the efficiency. This study aimed at developing and evaluating a biodegradable zein protein nanocarrier with the active ingredient (a.i.) cyantraniliprole (CNAP) for the control of <i>B. tabaci</i> in tomato (<i>Solanum lycopersicum)</i>. Synthesis of the nanoformulation (ZeinCNAP) resulted in spherical structures with an average hydrodynamic size of 143.06 ± 1.03 nm and a surface charge of 40.36 ± 0.7 mV remaining stable over 56 days. When plants were treated with three doses of ZeinCNAP (1/10, 1/2, and full) and commercial CNAP, followed by the release of adult whiteflies, the insect mortality by ZeinCNAP full dose (30 ± 0.9%) did not differ significantly from the commercial CNAP (42 ± 0.8%) 48 h after spraying. However, 72 h after spraying, the ZeinCNAP dose of 1/10, insect mortality (32 ± 1.1%) remained statistically equivalent to the commercial CNAP at full dose (49 ± 0.6%). When the plants were treated with commercial CNAP, ZeinCNAP, or water, aspects of fresh mass, photosynthetic parameters, and oxidative stress were analyzed at 24, 168, or 360 h after application, and no significant negative effects were noted in the analyzed parameters. The developed nanoinsecticide based on the zein platform and the active ingredient CNAP has significant potential for controlling on <i>B. tabaci</i> MEAM1 at reduced doses and can be considered safe for tomato plants. This work adds to a growing body of evidence demonstrating the potential of nanoscale carriers to significantly reduce the environmental burden associated with agrochemical use while still maintaining equivalent efficacy to conventional strategies.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 4","pages":"630–639 630–639"},"PeriodicalIF":2.3,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsagscitech.4c00751","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Linking Manganese Fractions and Organic Carbon in Soils of Contrasting Land Use Systems","authors":"Ashleigh Montgomery,&nbsp;Elizabeth Herndon and Sindhu Jagadamma*,&nbsp;","doi":"10.1021/acsagscitech.4c0054610.1021/acsagscitech.4c00546","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00546https://doi.org/10.1021/acsagscitech.4c00546","url":null,"abstract":"<p >Manganese (Mn) is an essential micronutrient that influences carbon (C) cycling by binding or oxidizing soil organic matter. Mn fractions in soil and their plant availability depend largely on soil pH, which is commonly altered by agricultural practices. Fractions of Mn in soils range from readily available (e.g., bioavailable Mn, exchangeable Mn) to stabilized (e.g., Mn-oxide minerals, Mn contained in silicates). The distribution of soil Mn with depth was assessed in contrasting land use systems (organic agriculture, conventional agriculture, and unmanaged forest) using a sequential extraction method that targets Mn fractions ranging in bioavailability. Both agricultural sites had similar amounts of total Mn but had available Mn (1–7% of total Mn) lower than that of the unmanaged forested site (15% of total Mn). Manganese and organic C were generally positively correlated, but this relationship depended on soil depth, Mn fractions, and concentrations, while land management change had little influence.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 3","pages":"316–320 316–320"},"PeriodicalIF":2.3,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143631656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Accumulation, Distribution, and Excretion of Different Silver Species in Tissues and Feces from Chickens and Pigs Fed with Silver-Based Nanomaterial Supplemented Feeds","authors":"Khaoula Ben-Jeddou*,&nbsp;Mariam Bakir,&nbsp;Maria S. Jimenez,&nbsp;Manuel Fondevila,&nbsp;Dino Metarapi,&nbsp;Martin Šala,&nbsp;Johannes T. van Elteren and Francisco Laborda,&nbsp;","doi":"10.1021/acsagscitech.4c0033810.1021/acsagscitech.4c00338","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00338https://doi.org/10.1021/acsagscitech.4c00338","url":null,"abstract":"<p >On the basis of two in vivo experiments involving pigs and chickens, where animals were fed with feeds supplemented with a silver–kaolin nanomaterial as a growth promoter, the fate of the ingested silver, its accumulation in different tissues and excretion, and its occurrence as ionic or particulate forms has been studied. Inductively coupled plasma mass spectrometry (ICP-MS) in conventional mode was used for the quantitative determination of total silver in the different tissues and feces. Results showed that silver accumulated in liver (0.15–5 mg kg^–1) and kidney (0.4 mg kg^–1) but not in muscle, while the major part of silver was eliminated through the feces (75–1026 mg kg^–1). Laser ablation coupled to ICP-MS (LA-ICP-MS) in a conventional mode revealed a preferential accumulation of silver in the outer layer of the liver lobules in pigs. LA-ICP-MS in single particle mode (LA-SP-ICP-MS) was applied to the analysis of tissues and feces to obtain speciation information about the presence of ionic and particulate silver in the samples. Silver found in the pig liver was present in ionic form, confirming that silver was absorbed in the intestine in this form. The analysis of pig feces confirmed the presence of both ionic silver and particles containing silver with average masses of 520 ag (equivalent to solid spherical silver nanoparticles of 50 nm diameter).</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 4","pages":"454–460 454–460"},"PeriodicalIF":2.3,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploiting Freshwater Mollusk Shell-Based CaO Nanoparticles: Preparation, Characterization, Insecticidal Activity, Antioxidant Potential, and Germination Enhancement in Greengram","authors":"K. Madesh,&nbsp;M. Kannan*,&nbsp;S. Inbaraj,&nbsp;S. Dineshkumar,&nbsp;K. Govindaraju,&nbsp;R. P. Soundarajan,&nbsp;M. Senthilkumar and N. Manikanda Boopathi,&nbsp;","doi":"10.1021/acsagscitech.4c0068710.1021/acsagscitech.4c00687","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00687https://doi.org/10.1021/acsagscitech.4c00687","url":null,"abstract":"<p >The present study utilizes a naturally available biomass waste of freshwater molluscan shells as a source for preparation of biocalcium oxide nanoparticles (CaO NPs) in the management of pulse beetle of <i>Callosobruchus maculatus</i> (<i>C. maculatus</i>) and growth enhancement of agro-morphological characters and antioxidant enzyme level in greengram seeds. The UV-DRS spectrum exhibited a pronounced absorption peak at 267 nm. The stability of the particles was evidenced by a zeta potential of −37.3 mV. FTIR exhibited peaks at 1412 and 868 cm^–1 are characteristic of the connection between the oxygen atom of CO₃^2–, Ca, and C–O bonding, which signifies the carbonation of the CaO NPs. The peaks appearing in the 359, 482, and 1089 cm^–1 bands of Raman spectra are the presence of calcium oxide. The XRD pattern was obtained and confirmed the polycrystalline nature of the CaO NPs, by a sharp peak and narrow spectrum. The TGA showed a major weight loss (∼43%) of freshwater mollusks’ shell between 695 and ∼800 °C. TEM and FE-SEM micrographs of CaO NPs found a granular morphology with an average size of 54.42 nm, respectively. The insecticidal activity of CaO NPs showed the LD₅₀ and LD₉₀ values of 12.58 and 406.64 mg/100 g of seeds, respectively. The pattern of mortality revealed that CaO NPs at a dose of 400 mg/100 g of seeds expressed 100% mortality on the third day after release of <i>C. maculatus</i>. CaO NPs interacted with <i>C. maculatus</i> by adherence on the body surface of the insect and caused sequential physical toxicity, dehydration, desiccation, and death. The <i>in silico</i> molecular docking studies of CaO with detoxifying enzymes revealed greater affinity and binding energies of −1.4, −1.4, and −1.3 kcal/mol in acetylcholinesterase, cytochrome-c, and glutathione-<i>S</i>-transferase, respectively. Furthermore, CaO NP-treated seeds showed significant enhancement in seed germination, shoot length, root length, seedling length, subroot numbers, and vigor index over deltamethrin and control. Seeds treated with CaO NPs produced more ROS than hydro-primed control, indicating that ROS are crucial for improving seed germination. CaO NPs in the greengram seedlings were confirmed through AAS. Leveraging biomass waste can be used as a novel source for sustainable control of insects and promising tools to enhance greengram seed for higher crop yield and bolstering food security.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 4","pages":"613–629 613–629"},"PeriodicalIF":2.3,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic Amino Acids Facilitate the Management of Plant Health and Stress Tolerance for a Modern Sustainable Approach","authors":"J.M.G.M.T. Karunarathne,&nbsp;E.K.W.W. Weerarathna,&nbsp;Xiao-Yu Hong,&nbsp;Ting Wen,&nbsp;Gefei Hao and W.M.W.W. Kandegama*,&nbsp;","doi":"10.1021/acsagscitech.4c0075310.1021/acsagscitech.4c00753","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00753https://doi.org/10.1021/acsagscitech.4c00753","url":null,"abstract":"<p >Accurate fertilization is of paramount importance in modern agriculture for maintaining the expected productivity. Continuous application of urea fertilizer to meet the nitrogen (N) requirement of plants causes detrimental effects; alternatively, shifting to eco-friendly biogenic hydrolysate (BH) rich in plant biostimulants is crucial. While many studies have sought to extract and characterize BHs rich in amino acids (AAs) in agriculture, systematic reviews on animal-based BHs and their effect on plant health and defense are relatively rare. This Review presents a comprehensive analysis that (1) identifies the importance of animal-based enriched BHs to plant growth, immunity development, and stress control; (2) presents the modulation of mechanisms inside plants; and (3) discusses recent case studies, successful applications, regulations, and potential future outcomes. This work paves the way for the identification of a pioneering sustainable approach for boosting crop productivity and resilience, opening the gate for a paradigm change in agriculture.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 3","pages":"294–315 294–315"},"PeriodicalIF":2.3,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143631657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silver Accumulation and Ionomic Profile Alterations in Pigs: Evaluation of Silver Nanoparticles as Feed Supplements and Potential Human Exposure","authors":"Bianca de Melo Santana,&nbsp;Guilherme Carvalho Tremiliosi,&nbsp;Bruno Lemos Batista,&nbsp;Lucilena Rebelo Monteiro,&nbsp;Amedea Barozzi Seabra,&nbsp;Joaquim Carlos Atra Goncalves,&nbsp;Hebert Silveira,&nbsp;Flávio de Aguiar Coelho,&nbsp;Laya Kannan Silva Alves,&nbsp;Cesar Augusto P. Garbossa and Camila Neves Lange*,&nbsp;","doi":"10.1021/acsagscitech.4c0062410.1021/acsagscitech.4c00624","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00624https://doi.org/10.1021/acsagscitech.4c00624","url":null,"abstract":"<p >The antimicrobial properties of silver nanoparticles (AgNPs) in animal feed have drawn increasing attention as a potential alternative to antibiotics. However, concerns about bioaccumulation and possible effects on mineral homeostasis require a thorough evaluation. This study investigates the bioaccumulation of Ag in various tissues and ionomic alterations in pigs fed AgNPs complexed with carbohydrates (AgNPs@Carb). Silver concentrations were measured in tissues, such as the liver, kidney, spleen, heart, and cecal contents, at different time points following the withdrawal of the nanoparticle-supplemented diet. Principal component analysis (PCA) assessed the concentrations of 18 elements across 10 tissues. Results indicate that silver primarily accumulates in the liver and cecal contents, with varying clearance rates across tissues. The silver estimated daily intake (EDI) for human consumption was evaluated, revealing low values across all tissues. This suggests that potential exposure to Ag through the consumption of edible tissues from animals supplemented with AgNPs is minimal and does not pose an immediate health risk. Significant changes were also observed in the ionomic profiles, suggesting that AgNPs disrupt trace element homeostasis. These findings underscore the importance of understanding both the biodistribution of silver nanoparticles and their potential long-term impact on animal health and human consumers.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 4","pages":"513–522 513–522"},"PeriodicalIF":2.3,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsagscitech.4c00624","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocatalyzed Synthesis of Benzoyl and Cinnamoylamides Inspired by Rice Phytoalexins","authors":"Cecilia Pinna,&nbsp;Luca Nespoli,&nbsp;Giulia Brioschi,&nbsp;Andrea Kunova,&nbsp;Paolo Cortesi,&nbsp;Piera Anna Martino,&nbsp;Francesco Molinari,&nbsp;Loana Musso,&nbsp;Sabrina Dallavalle,&nbsp;Martina L. Contente* and Andrea Pinto,&nbsp;","doi":"10.1021/acsagscitech.4c0038010.1021/acsagscitech.4c00380","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00380https://doi.org/10.1021/acsagscitech.4c00380","url":null,"abstract":"<p >Worldwide, phytopathogenic fungi, bacteria, and viruses are responsible for huge crop losses each year, threatening agricultural progress and food security and causing massive economic damages. <i>Pyricularia oryzae</i> represents one of the most dangerous fungal phytopathogens being the cause of rice blast, a highly destructive disease widely distributed across the world. In this critical context, good agricultural practices necessarily need to be supported using novel, effective, and sustainable agrochemicals. It is known that plants naturally counteract exogenous infections by synthesizing defense secondary metabolites, known as phytoalexins. Inspired by <i>N</i>-benzoyltryptamine and <i>N</i>-cinnamoyltryptamine, two phytoalexins found in <i>Oryza sativa</i>, we designed a collection of tryptamine-based derivatives. The compounds were synthesized exploiting an enzymatic approach, using <i>Candida antarctica Lipase B</i> (CaL-B) as a biocatalyst and <i>tert</i>-amyl alcohol (<i>t</i>-AA) as an unconventional green solvent. The activity was evaluated against a panel of different phytopathogenic fungi as well as selected Gram-negative and Gram-positive bacteria. The obtained results pave the way for novel nature-inspired products as a valuable alternative to currently available pesticides.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 4","pages":"461–467 461–467"},"PeriodicalIF":2.3,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsagscitech.4c00380","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Broad-Specificity Immunoassays for Detecting Prochloraz and Its Metabolites in Grapes and Water","authors":"Yun Zhao,&nbsp;Peipei Li*,&nbsp;A. M. Abd El-Aty,&nbsp;Xingmei Lei,&nbsp;Jing Zhao,&nbsp;Lingyuan Xu,&nbsp;Song Gao,&nbsp;Jia Li,&nbsp;Yongxin She,&nbsp;Fen Jin,&nbsp;Jing Wang,&nbsp;Lufei Zheng*,&nbsp;Bruce D. Hammock and Maojun Jin*,&nbsp;","doi":"10.1021/acsagscitech.4c0064810.1021/acsagscitech.4c00648","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00648https://doi.org/10.1021/acsagscitech.4c00648","url":null,"abstract":"<p >This study aimed to develop broad-specificity antibodies for detecting prochloraz and its metabolites. Initially, three haptens were designed and identified through computational chemical methods, and the hapten H2 was the optimal candidate. Using H2-BSA as an immunogen, the monoclonal antibody 9C3 was prepared to recognize PCH and two metabolites, BTS44595 and BTS44596. The broad-specificity recognition mechanism revealed that the “2,4,6-trichlorophenyl” moiety of the three targets is completely encapsulated in the binding pocket of mAb 9C3 and that hydrophobic forces play crucial roles in binding. Furthermore, an indirect enzyme-linked immunosorbent assay and a colloidal gold immunochromatographic assay for detecting prochloraz residues in grapes and river water samples were developed. The IC₅₀ of ic-ELISA was 8.08–15.07 ng/mL, with recovery rates ranging from 73.80% to101.13%. In addition, the visual detection limit of GICA was 250–500 ng/mL, with IC₅₀ values ranging from 98.04 to 126.29 ng/mL and CRs ranging from 67.2 to 78.02%. The accuracy and precision of both immunoassays were validated by LC–MS/MS.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 4","pages":"528–541 528–541"},"PeriodicalIF":2.3,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Production of Ergothioneine in Yarrowia lipolytica through Combined Metabolic and Enzyme Engineering","authors":"Linfeng Hu,&nbsp;Mengsu Liu,&nbsp;Qihang Chen,&nbsp;Minyu Yue,&nbsp;Weizhu Zeng,&nbsp;Jingwen Zhou,&nbsp;Changtai Zhang and Sha Xu*,&nbsp;","doi":"10.1021/acsagscitech.4c0073010.1021/acsagscitech.4c00730","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00730https://doi.org/10.1021/acsagscitech.4c00730","url":null,"abstract":"<p >Ergothioneine (EGT) is a sulfur-containing histidine derivative with antioxidant, antiaging, and antidepressant properties. It is widely used in the food, medicine, and cosmetics industries. However, both the chemical synthesis and biological extraction of EGT are constrained by high cost and low yield. In this study, EGT synthetases EGT1 and EGT2 from <i>Trichoderma reesei</i> were expressed in <i>Yarrowia lipolytica</i> using various expression vectors. Several key sites in TrEgt1 were identified by alanine scanning mutagenesis, mutated to hydrophobic amino acids, and the EGT titer of the Y786A-A492 V-<i>TrEGT1</i> mutant was 2.41 times higher than that of the wild-type strain. To improve the supply of precursor amino acids, the associated network was divided into four modules, which have been systematically enhanced. Combining the above modifications resulted in an engineered strain that produced 516.3 mg/L EGT in multiwell plates. Fermentation was optimized in a 5 L bioreactor, and EGT accumulation reached 9.3 g/L after 168 h, with a production intensity of 55.35 mg/L/h, the highest reported to date. These strategies provided references for the construction of EGT-producing microorganisms.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 4","pages":"603–612 603–612"},"PeriodicalIF":2.3,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}