Bianca C. Silva, Brena G. de Souza, Amanda A. Bertolazi, Arthur R. Pêgos, Cristina Cruz, Juliana Melo, Olubukola O. Babalola, César A. Krohling and Alessandro C. Ramos*,
{"title":"","authors":"Bianca C. Silva, Brena G. de Souza, Amanda A. Bertolazi, Arthur R. Pêgos, Cristina Cruz, Juliana Melo, Olubukola O. Babalola, César A. Krohling and Alessandro C. Ramos*, ","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 6","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":2.3,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acsagscitech.5c00023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144357219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mariana G. Brondi, Camila Florencio, Vanessa M. Vasconcellos, Caue Ribeiro and Cristiane S. Farinas*,
{"title":"","authors":"Mariana G. Brondi, Camila Florencio, Vanessa M. Vasconcellos, Caue Ribeiro and Cristiane S. Farinas*, ","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 6","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":2.3,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acsagscitech.5c00189","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144422322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Rice Dryland Cultivation Promotes Beneficial Rhizosphere Microorganisms and Root Metabolites, Enhancing Rhizosphere Soil Quality","authors":"Wenjie Gao, Yu He, Zepeng Chen*, Sheng Wang, Guangsheng He, Kaiwei Yao, Israt Jahan, Jun Wang, Zhiqiang Wen, Jian Zhang, Liang Shuai, Ping Li* and Jian Yan*, ","doi":"10.1021/acsagscitech.4c0073710.1021/acsagscitech.4c00737","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00737https://doi.org/10.1021/acsagscitech.4c00737","url":null,"abstract":"<p >China is currently the leading producer and consumer of rice globally, and soil quality has a decisive impact on rice growth, including metabolite accumulation and plant disease resistance. Despite the importance of these interactions, limited research exists on the relationship between rhizosphere microorganisms and secondary metabolite profiles across different rice cultivars. To address this gap, 16s rRNA and ITS sequencing and metabolomics were used to investigate the changes in rhizosphere microorganisms and root metabolism in different rice cultivars under dryland conditions. The results showed that rice dry farming can improve soil quality by improving soil chemical properties and soil enzyme activities and increasing beneficial soil microorganisms. Compared to SLHN, the organic carbon content notably increased under dry farming, with YHSM exhibiting the highest rise at 182.90%. Soil urease (S-UE) and acid phosphatase (S-ACP) activities in YNSM also increased significantly by 116.90 and 19.65%, respectively. Additionally, rice drought farming can also increase the content of root metabolites and improve rice drought resistance. These findings offer valuable insights into the mechanism of rice under drought conditions.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 6","pages":"1005–1013 1005–1013"},"PeriodicalIF":2.3,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Determination of Transgenes in Crops by a Strategy Combining Cas9 Targeting with Amplification-Free Long-Read Nanopore Sequencing","authors":"Jumpei Narushima, Satoko Yoshiba*, Aoi Hosokawa, Miyu Sugino, Keisuke Soga, Yohei Sugano, Reona Takabatake, Kazuhiko Nishimura, Kazunari Kondo and Norihito Shibata*, ","doi":"10.1021/acsagscitech.5c0017510.1021/acsagscitech.5c00175","DOIUrl":"https://doi.org/10.1021/acsagscitech.5c00175https://doi.org/10.1021/acsagscitech.5c00175","url":null,"abstract":"<p >Considering any transgene in crops generated through genetic engineering is a potential safety concern, reliable methods for detecting transgenes are needed. Although whole-genome sequencing (WGS) using next-generation sequencing technology is a promising method for comprehensively detecting transgenes, its utility depends on the production of large amounts of sequencing data. Transgene enrichment may address this issue. We herein propose a strategy that combines amplification-free long-read nanopore sequencing with the CRISPR/Cas9 system. Specifically, the transgene is selectively enriched and analyzed by ligating an adapter after a target element of the transgene is cleaved by Cas9, which is followed by nanopore sequencing. In this study, we targeted three elements of transgenes frequently used to develop genetically modified (GM) and genome-edited (GE) crops. Our data showed that this strategy can detect transgenes more efficiently than WGS. Therefore, it may be useful in food safety assessment for GM and GE crops.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 6","pages":"1168–1177 1168–1177"},"PeriodicalIF":2.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Matteo Radice*, Andrea Durofil, Sandra Ríos-Núñez, Roberta Tardugno, Stefano Manfredini, Reinier Abreu Naranjo and Laura Scalvenzi,
{"title":"Unlocking the Anti-Phytopathogenic Potential of Essential Oils: A Systematic Review of Plant-Based Botrytis cinerea Management for Regenerative Viticulture and Horticultural Products","authors":"Matteo Radice*, Andrea Durofil, Sandra Ríos-Núñez, Roberta Tardugno, Stefano Manfredini, Reinier Abreu Naranjo and Laura Scalvenzi, ","doi":"10.1021/acsagscitech.5c0015110.1021/acsagscitech.5c00151","DOIUrl":"https://doi.org/10.1021/acsagscitech.5c00151https://doi.org/10.1021/acsagscitech.5c00151","url":null,"abstract":"<p >In the context of regenerative agriculture, essential oils (EOs) are emerging as promising alternatives for managing <i>Botrytis cinerea</i>, a highly destructive phytopathogen responsible for significant agricultural losses. This systematic review analyzed 76 eligible articles highlighting increased research activity since 2020, predominantly addressing <i>in vitro</i> antifungal activity, with emerging interest in nanotechnological formulations and field trials. Thymol- and carvacrol-rich EOs demonstrate the highest efficacy. Preliminary findings on microencapsulation and nanoemulsions indicate advancements in stability, solubility, and controlled release, enhancing the EO effectiveness. However, the development of EO-based biopesticides faces challenges due to volatility, lipophilicity, and production costs. Addressing these limitations through innovative formulations and standardizing extraction processes could pave the way for integrating EOs into sustainable pest management systems in viticulture and horticulture. This review also provides critical insights into the current technological readiness levels of EO-based anti-<i>Botrytis</i> products, emphasizing the need for interdisciplinary research to transition laboratory findings into viable commercial solutions.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 6","pages":"930–948 930–948"},"PeriodicalIF":2.3,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bianca C. Silva, Brena G. de Souza, Amanda A. Bertolazi, Arthur R. Pêgos, Cristina Cruz, Juliana Melo, Olubukola O. Babalola, César A. Krohling and Alessandro C. Ramos*,
{"title":"Isolation and Characterization of Glyphosate-Tolerant Bacteria from a Local Coffee Plantation in Espírito Santo, Brazil","authors":"Bianca C. Silva, Brena G. de Souza, Amanda A. Bertolazi, Arthur R. Pêgos, Cristina Cruz, Juliana Melo, Olubukola O. Babalola, César A. Krohling and Alessandro C. Ramos*, ","doi":"10.1021/acsagscitech.5c0002310.1021/acsagscitech.5c00023","DOIUrl":"https://doi.org/10.1021/acsagscitech.5c00023https://doi.org/10.1021/acsagscitech.5c00023","url":null,"abstract":"<p >This study aimed to isolate glyphosate-tolerant bacteria and characterize their plant-beneficial traits. Using a bioprospecting and glyphosate enrichment methodology, we isolated 35 bacterial strains that were tested for tolerance to glyphosate on solid and liquid media (0, 11, 22, 44, and 88 g L<sup>–1</sup> of glyphosate). Sixteen isolates with high tolerance to glyphosate (≥22 g L<sup>–1</sup>) were identified and characterized by their ability to fix atmospheric nitrogen, solubilize inorganic phosphate, and produce indole-3-acetic acid (IAA). After similarity analysis and compatibility testing, a consortium composed of three bacteria (<i>Priestia</i> sp.; <i>Achromobacter</i> sp.; <i>Pseudomonas</i> sp.) were selected to be applied in a model plant (tomato) and Arabica coffee beans. The bacterial consortium promoted the germination (96.6%) and development of tomato seedlings, while also degrading residual glyphosate (31.25%) in coffee beans, as evidenced by the reduced levels of the herbicide. These results demonstrate the bacterial consortium’s potential to reduce glyphosate residues and sustainably increase plant productivity.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 6","pages":"1071–1085 1071–1085"},"PeriodicalIF":2.3,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsagscitech.5c00023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Stella F. Valle, Amanda S. Giroto, Gelton G. F. Guimarães and Caue Ribeiro*,
{"title":"","authors":"Stella F. Valle, Amanda S. Giroto, Gelton G. F. Guimarães and Caue Ribeiro*, ","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 5","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":2.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acsagscitech.5c00025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144390580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}