{"title":"Synthesis and Herbicidal Activity of 3-Phenyl-5-oxy-benzothiazole-2-one Derivatives","authors":"Luyao Chen, Qianhong Liu, Jinjin Wang, Shaopeng Wei* and Zhiqin Ji*, ","doi":"10.1021/acsagscitech.5c0010710.1021/acsagscitech.5c00107","DOIUrl":"https://doi.org/10.1021/acsagscitech.5c00107https://doi.org/10.1021/acsagscitech.5c00107","url":null,"abstract":"<p >A series of 3-phenyl-5-oxy-benzothiazole-2-ones were designed based on the structure of 3-(2-pyridyl)-benzothiazol-2-one by using positional isomerization and bioisosteric replacement strategies. Starting from 4-chloro-3-nitroanisole, the title compounds were prepared via cyclization, oxidation, nucleophilic aromatic substitution, demethylation, and O-alkylation reactions. The herbicidal activities of 31 compounds against Echinochloa crus-galli and Portulaca oleracea were evaluated by the Petri dish assay. The results indicated that <b>VI-2</b>, <b>VI-3</b>, <b>VI-4</b>, <b>VIII-13</b>, <b>VIII-14</b>, <b>VIII-15</b>, <b>VIII-16</b>, <b>VIII-18</b>, <b>VIII-19</b>, <b>VIII-24</b>, and <b>VIII-25</b> completely inhibited the growth of <i>P. oleracea</i> at 10 μg/mL. <b>VIII-18</b> showed the most potent activity against <i>P. oleracea</i>, and the inhibition rate is 100% even at 1 μg/mL. Barnyard grass is relatively insensitive to most of the test compounds, and only <b>VI-2</b>, <b>VIII-16</b>, and <b>VIII-18</b> showed a strong inhibitory effect at 10 μg/mL. The herbicidal activities of compounds <b>VIII-13</b>, <b>VIII-18</b>, and <b>VIII-19</b> against a panel of weeds were further evaluated by pot experiments in glasshouse. The results showed that <b>VIII-13</b>, <b>VIII-18</b>, and <b>VIII-19</b> exhibited good herbicidal activity against broadleaf weeds such as Abutilon theophrasti, <i>Solanum nigrum</i>, Amaranthus retroflexus, and <i>Chenopodium album</i> at 30 and 15 g/ha, whereas they only showed a moderately inhibitory activity against grass weeds such as E. crus-galli, and Digitaria sanguinalis, as well as geraniaceae weeds like Geranium carolinianum. Concurrently, we employed molecular simulations to obtain further insights into the binding mechanism with <i>Nt</i>PPO. In general, 3-phenyl-5-oxy-benzothiazole-2-ones were discovered as a type of promising candidates for the management of broadleaf weeds, and they provide a novel structural scaffold for the development of herbicides.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 5","pages":"896–902 896–902"},"PeriodicalIF":2.3,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144083591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Beyond Boundaries: Art, Science, and the Power of Representation","authors":"Emily A. Dustman*, ","doi":"10.1021/acsagscitech.5c0007810.1021/acsagscitech.5c00078","DOIUrl":"https://doi.org/10.1021/acsagscitech.5c00078https://doi.org/10.1021/acsagscitech.5c00078","url":null,"abstract":"","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 3","pages":"292–293 292–293"},"PeriodicalIF":2.3,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143631529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative Analysis of Protein Extraction Protocols for Olive Leaf Proteomics: Insights into Differential Protein Abundance and Isoelectric Point Distribution.","authors":"Bihter Uçar, Merve Öztuğ, Mahmut Tör, Nurçin Çelik-Öztürk, Filiz Vardar, Birsen Cevher-Keskin","doi":"10.1021/acsagscitech.4c00642","DOIUrl":"10.1021/acsagscitech.4c00642","url":null,"abstract":"<p><p>Plant proteomics studies face two major challenges: limited databases due to the need for sequenced genomes and the difficulty in obtaining high-quality protein extracts. Olive (<i>Olea europaea</i>), a key species in Mediterranean flora known for its rich biochemical content, presents additional complexity due to its lipidic structure and high levels of inhibitory compounds that hinder protein extraction. Consequently, various studies have focused on optimizing the protein extraction methods for olives. While different extraction protocols exist for leaf proteome analysis, their compatibility with LC-MS/MS has been scarcely studied. This work was carried out to compare three protein extraction protocols for LC-MS/MS analysis using olive (<i>O. europaea</i> L) leaf tissue. Denaturing SDS (Method A), physiological CHAPS (Method B), and phenolic TCA/acetone (Method C) were evaluated with LC-MS/MS data. The quantitative comparisons of the three extraction methods revealed that Protocol A gave the greatest yields. According to the results obtained, Protocol A uniquely identified 77 proteins, Protocol B identified 10 unique proteins, and Protocol C identified 19 unique proteins. Similarly, the peptide sequence analysis showed that Protocol A uniquely identified 208 peptide sequences, Protocol B identified 29, and Protocol C identified 36. Moreover, reversed-phase high-performance liquid chromatography (RP-HPLC) results suggest that Method A may be more efficient in removing and retaining hydrophobic proteins. Overall, Protocol A demonstrated greater sensitivity, efficiency, and reproducibility in LC-MS/MS analysis.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 5","pages":"739-749"},"PeriodicalIF":2.3,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12093291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144129723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bihter Uçar, Merve Öztuğ, Mahmut Tör, Nurçin Çelik-Öztürk, Filiz Vardar and Birsen Cevher-Keskin*,
{"title":"Comparative Analysis of Protein Extraction Protocols for Olive Leaf Proteomics: Insights into Differential Protein Abundance and Isoelectric Point Distribution","authors":"Bihter Uçar, Merve Öztuğ, Mahmut Tör, Nurçin Çelik-Öztürk, Filiz Vardar and Birsen Cevher-Keskin*, ","doi":"10.1021/acsagscitech.4c0064210.1021/acsagscitech.4c00642","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00642https://doi.org/10.1021/acsagscitech.4c00642","url":null,"abstract":"<p >Plant proteomics studies face two major challenges: limited databases due to the need for sequenced genomes and the difficulty in obtaining high-quality protein extracts. Olive (<i>Olea europaea</i>), a key species in Mediterranean flora known for its rich biochemical content, presents additional complexity due to its lipidic structure and high levels of inhibitory compounds that hinder protein extraction. Consequently, various studies have focused on optimizing the protein extraction methods for olives. While different extraction protocols exist for leaf proteome analysis, their compatibility with LC–MS/MS has been scarcely studied. This work was carried out to compare three protein extraction protocols for LC–MS/MS analysis using olive (<i>O. europaea</i> L) leaf tissue. Denaturing SDS (Method A), physiological CHAPS (Method B), and phenolic TCA/acetone (Method C) were evaluated with LC–MS/MS data. The quantitative comparisons of the three extraction methods revealed that Protocol A gave the greatest yields. According to the results obtained, Protocol A uniquely identified 77 proteins, Protocol B identified 10 unique proteins, and Protocol C identified 19 unique proteins. Similarly, the peptide sequence analysis showed that Protocol A uniquely identified 208 peptide sequences, Protocol B identified 29, and Protocol C identified 36. Moreover, reversed-phase high-performance liquid chromatography (RP-HPLC) results suggest that Method A may be more efficient in removing and retaining hydrophobic proteins. Overall, Protocol A demonstrated greater sensitivity, efficiency, and reproducibility in LC–MS/MS analysis.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 5","pages":"739–749 739–749"},"PeriodicalIF":2.3,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsagscitech.4c00642","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144083517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bihua Xia, Mingxuan Hou, Runyi Qu, Yang Wang, Ting Li, Mingqing Chen, Shibo Wang and Weifu Dong*,
{"title":"Preparation of a Sericin Composite Film Loaded with Rosmarinic Acid-Allicin Antibacterial Nanoparticles for Active Food Packaging Application","authors":"Bihua Xia, Mingxuan Hou, Runyi Qu, Yang Wang, Ting Li, Mingqing Chen, Shibo Wang and Weifu Dong*, ","doi":"10.1021/acsagscitech.4c0078310.1021/acsagscitech.4c00783","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00783https://doi.org/10.1021/acsagscitech.4c00783","url":null,"abstract":"<p >This paper fabricated a new type of sericin composite film loaded with rosmarinic acid-allicin nanoparticles by the precipitation polymerization method and casting film method. Rosmarinic acid-allicin nanoparticles were used as antibacterial agents, and sericin was used as the carrier material of rosmarinic acid-allicin nanoparticles. The structures of rosmarinic acid-allicin nanoparticles and rosmarinic acid-allicin nanoparticle-sericin composite films were characterized by a Fourier transform infrared spectrometer, a UV–visible spectrophotometer, and a scanning electron microscope. The mechanical strength (0.56 MPa tensile strength and 152.1% strain rate), UV shielding performance (>80%), antioxidant performance (>90%), antibacterial performance (>99%), and biocompatibility (>85%) of rosmarinic acid-allicin nanoparticle-sericin composite films were researched. Test results demonstrated that this rosmarinic acid-allicin nanoparticle-sericin composite film exhibited high UV shielding performance, high antioxidant performance, high biocompatibility, and high antibacterial performance. In addition, this rosmarinic acid-allicin nanoparticle-sericin composite film can delay food spoilage as a packaging material effectively. This rosmarinic acid-allicin nanoparticle-sericin composite film can be used in the field of food packaging materials.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 4","pages":"640–648 640–648"},"PeriodicalIF":2.3,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sania Zaib*, Samina N. Shakeel, Arif Ali and Kashif Akhtar,
{"title":"Quantitative RT-PCR Analysis of Plant Growth-Promoting Rhizobacteria-Induced Transcriptional Responses in Barley (Hordeum vulgare L.) under High Salt and Drought Stress Conditions","authors":"Sania Zaib*, Samina N. Shakeel, Arif Ali and Kashif Akhtar, ","doi":"10.1021/acsagscitech.4c0021310.1021/acsagscitech.4c00213","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00213https://doi.org/10.1021/acsagscitech.4c00213","url":null,"abstract":"<p >Salinity and drought are two major environmental stresses that significantly impact global agricultural productivity. Plant growth-promoting rhizobacteria (PGPR) are essential bioinoculants that contribute to sustainable agriculture by enhancing plant resilience to such stresses. Given the importance of motility for effective rhizosphere colonization, this study initially screened various PGPR strains for their motility and chemotactic behaviors. Except for <i>Pseudomonas fluorescens</i> F113, all of the tested strains exhibited high motility. Based on these results, two strains, i.e., <i>P. fluorescens</i> SBW25 and <i>Pseudomonas putida</i> KT2440 were selected for further evaluation of their ability to promote barley growth under abiotic stress conditions. Barley plants inoculated with selected PGPR strains were subjected to salt (200 mM and 1000 mM) and drought stress conditions, and their biochemical and molecular responses were compared to noninoculated plants. Biochemical analysis revealed that inoculated plants have significantly higher levels of osmolytes such as soluble sugars (78 mg/g in SBW25), proline (12 mg/g in SBW25), free amino acids (5.8 mg/g in SBW25), and antioxidant enzymes (POD: 0.72, CAT: 0.78 U/mg in SBW25, and APx: 4.1 U/mg in KT2440) compared to controls under stress conditions. At the molecular level, qRT-PCR analysis revealed that PGPR strain <i>P. putida</i> KT2440 significantly upregulated key genes in the jasmonic acid defense pathway, including <i>FAD3</i> (∼3900%), <i>LOX1</i> (1600%), <i>AOS</i> (380%), and <i>AOC</i> (540%) under high salt (1000 mM) stress. Both PGPR strains also induced a marked increase in ethylene biosynthesis gene <i>ACCO</i> expression (3400% and 1500%) compared to control (∼200%), under high salt stress, while downregulated <i>ACCS</i> (50% and 95.2%) and <i>ACCO</i> (50% and 93.8%) under drought stress, as opposed to their controls (<i>ACCS</i>: 2600%, <i>ACCO</i>: 10,600%). Additionally, the <i>MAPKK</i> gene (3250%) indicated a significantly strong induction under high salt stress with <i>P. putida</i> KT2440. Other stress-related genes, such as <i>NHX1</i> (8500% in <i>P. putida</i> KT2440), <i>NRT2.2</i> (1800% in <i>P. fluorescens</i> SBW25), <i>CAT2</i> (1200% in <i>P. fluorescens</i>), and <i>GR</i> (420% in <i>P. putida</i>), were also upregulated under different stress conditions. Overall, most of the phytohormone biosynthesis genes were upregulated under high salt (1000 mM) and downregulated under drought stress conditions. In summary, our results demonstrated that these PGPR strains can enhance salinity and drought tolerance in barley by modulating key defense pathways, ion and nitrate transporters, and antioxidant enzymes. These findings highlight the potential use of these <i>Pseudomonas</i> strains in improving stress tolerance in barley and related crops. Additionally, the candidate genes identified in this study could serve as valuable markers for breeding stress-toler","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 5","pages":"687–700 687–700"},"PeriodicalIF":2.3,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144083559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ziyun Ru, Mengsu Liu, Qihang Chen, Hongbiao Li, Yang Ning, Weizhu Zeng and Jingwen Zhou*,
{"title":"High-Level De Novo Production of (2S)-Naringenin in Yarrowia lipolytica Using Metabolic and Enzyme Engineering","authors":"Ziyun Ru, Mengsu Liu, Qihang Chen, Hongbiao Li, Yang Ning, Weizhu Zeng and Jingwen Zhou*, ","doi":"10.1021/acsagscitech.4c0072910.1021/acsagscitech.4c00729","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00729https://doi.org/10.1021/acsagscitech.4c00729","url":null,"abstract":"<p >(2<i>S</i>)-Naringenin, a type of natural flavonoid found in rutaceae plants, has antibacterial, anti-inflammatory, antioxidant, and lipid-lowering effects. However, biosynthesizing (2<i>S</i>)-naringenin results in an inadequate precursor supply and low catalytic efficiency of chalcone synthase (CHS). Here, <i>Yarrowia lipolytica</i> was developed by enzyme and metabolic engineering for high-level (2<i>S</i>)-naringenin production. In enzyme engineering, the catalytic pockets were identified by molecular docking, alanine scanning and iterative mutation were performed according to the conformation obtained, and the mutation results were simulated by molecular dynamics. The results showed that the mutation caused the enzyme and substrate to bind more closely, thus increasing the titer of (2<i>S</i>)-naringenin. In metabolic engineering, the number of copies of CHS and CHI (encoding chalcone isomerase), the key genes in the metabolic pathway of (2<i>S</i>)-naringenin, was increased to four to promote the synthesis of (2<i>S</i>)-naringenin, which resulted in the production of (2<i>S</i>)-naringenin reaching 246.4 mg/L. In addition, by introducing key genes of the shikimate pathway and highly active mutants to remove feedback inhibition and by introducing unnatural ways to enhance malonyl-CoA supply, these strategies resulted in a titer of 615.0 mg/L for (2<i>S</i>)-naringenin. However, <i>p</i>-coumaric acid still accumulated, and a multicopy integration tool was further used to integrate the synthesis genes of the downstream metabolic pathway to improve the conversion of the precursor to (2<i>S</i>)-naringenin. Through the combination of enzyme engineering and metabolic engineering, the titer of (2<i>S</i>)-naringenin increased from the initial 24.1 to 776.3 mg/L. Finally, using fed-batch fermentation, a remarkable amount of (2<i>S</i>)-naringenin (8.65 g/L) was obtained. This study reports the highest quantity of (2<i>S</i>)-naringenin synthesized in <i>Y. lipolytica</i> while facilitating green and sustainable methodologies for industrial production.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 5","pages":"784–793 784–793"},"PeriodicalIF":2.3,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144083558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pushp Sheel Shukla*, Izabela Michalak*, Anne-Sophie Burlot, Nathalie Bourgougnon, Bruno Daridon, Domenico Ronga, Anicia Hurtado and Alan T. Critchley,
{"title":"Phycobiostimulants: Next-Generation Sustainable Agricultural Inputs","authors":"Pushp Sheel Shukla*, Izabela Michalak*, Anne-Sophie Burlot, Nathalie Bourgougnon, Bruno Daridon, Domenico Ronga, Anicia Hurtado and Alan T. Critchley, ","doi":"10.1021/acsagscitech.4c0077710.1021/acsagscitech.4c00777","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00777https://doi.org/10.1021/acsagscitech.4c00777","url":null,"abstract":"<p >Plant biostimulants are a novel class of naturally derived agricultural inputs. The grouping of categories within the regulatory term “biostimulant” contains disparate inputs, such as humates, amino acids, bacteria, and algal extracts (i.e., many extraction processes applied to a relatively small group of selected seaweeds/microalgae). All seem to have the ability to improve the health and abiotic stress resistance of the treated plants. Even extracts of seaweeds (phycobiostimulants) are considerably different from one another (they are not all the same yet commonly referred to as seaweed extract, even in peer-reviewed publications), and current commercial offerings are available from a select group of green, red, and brown seaweeds. Commercial formulations combine different seaweed extracts and increasingly leverage synergies by blending biostimulants with other agricultural inputs and agrochemicals. Extensive research highlights that various algal-based extracts can effectively support both abiotic and biotic stress resistance when applied to both treated terrestrial plants and other cultivated algae. This article explores the scientific nuances of phycobiostimulants, challenging traditional regulatory classifications and emphasizing their essential role in sustainable agronomy, growing phyconomy, and global food security.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 4","pages":"424–453 424–453"},"PeriodicalIF":2.3,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Olamide R Ogundele, Mary Fakunle, Riley Pope-Buss, Jacob Churchman, Blessing Akinwande, Naum Kirwa, Polycarp C Ofoegbu, Cyren M Rico
{"title":"Physiological and Metabolic Responses of Wheat (<i>Triticum aestivum</i> L.) after One-Generation Exposure to Perfluorooctanesulfonic Acid (PFOS).","authors":"Olamide R Ogundele, Mary Fakunle, Riley Pope-Buss, Jacob Churchman, Blessing Akinwande, Naum Kirwa, Polycarp C Ofoegbu, Cyren M Rico","doi":"10.1021/acsagscitech.4c00722","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00722","url":null,"abstract":"<p><p>The pattern of plant responses, particularly on the seeds/grains metabolite profile, after generational exposure to contaminants is not well documented. Seeds from wheat cultivated in soil amended with PFOS at 0 and 25 mg/kg in the first generation were grown in clean soil to produce daughter plants and seeds in the second generation and assigned treatment combinations of 0-0 mg/kg PFOS and 25-0 mg/kg PFOS. Plant stress and responses including growth and biomass production, chlorophyll content, lipid peroxidation, and enzyme activity were measured over a short exposure period (21 days growth period). Biomass yields, elemental concentration, and grain metabolites were also measured after a long exposure period (92 days growth period). The daughter plants exhibited decreased chlorophyll content and lipid peroxidation in a short exposure period. The elemental concentrations were mostly not affected except for changes in microelements, except B, in the grains. In the metabolomics analysis, grains harvested from plants previously exposed to PFOS (i.e., 25-0 mg/kg PFOS) showed increased abundances of sucrose, linolenic acid, tryptophan, inositol-4-monophosphate, and ferulic acid, perhaps in response to adaptation to former stress. The current findings seem to suggest that one-generation exposure to PFOS does not cause detrimental effects on the next generation after the cessation of exposure. The results provide insights into the effects of generational exposure of plants to PFOS.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 4","pages":"593-602"},"PeriodicalIF":2.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12015812/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144059836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Olamide R. Ogundele, Mary Fakunle, Riley Pope-Buss, Jacob Churchman, Blessing Akinwande, Naum Kirwa, Polycarp C. Ofoegbu and Cyren M. Rico*,
{"title":"Physiological and Metabolic Responses of Wheat (Triticum aestivum L.) after One-Generation Exposure to Perfluorooctanesulfonic Acid (PFOS)","authors":"Olamide R. Ogundele, Mary Fakunle, Riley Pope-Buss, Jacob Churchman, Blessing Akinwande, Naum Kirwa, Polycarp C. Ofoegbu and Cyren M. Rico*, ","doi":"10.1021/acsagscitech.4c0072210.1021/acsagscitech.4c00722","DOIUrl":"https://doi.org/10.1021/acsagscitech.4c00722https://doi.org/10.1021/acsagscitech.4c00722","url":null,"abstract":"<p >The pattern of plant responses, particularly on the seeds/grains metabolite profile, after generational exposure to contaminants is not well documented. Seeds from wheat cultivated in soil amended with PFOS at 0 and 25 mg/kg in the first generation were grown in clean soil to produce daughter plants and seeds in the second generation and assigned treatment combinations of 0–0 mg/kg PFOS and 25–0 mg/kg PFOS. Plant stress and responses including growth and biomass production, chlorophyll content, lipid peroxidation, and enzyme activity were measured over a short exposure period (21 days growth period). Biomass yields, elemental concentration, and grain metabolites were also measured after a long exposure period (92 days growth period). The daughter plants exhibited decreased chlorophyll content and lipid peroxidation in a short exposure period. The elemental concentrations were mostly not affected except for changes in microelements, except B, in the grains. In the metabolomics analysis, grains harvested from plants previously exposed to PFOS (i.e., 25–0 mg/kg PFOS) showed increased abundances of sucrose, linolenic acid, tryptophan, inositol-4-monophosphate, and ferulic acid, perhaps in response to adaptation to former stress. The current findings seem to suggest that one-generation exposure to PFOS does not cause detrimental effects on the next generation after the cessation of exposure. The results provide insights into the effects of generational exposure of plants to PFOS.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"5 4","pages":"593–602 593–602"},"PeriodicalIF":2.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsagscitech.4c00722","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}