Journal of chromatography open最新文献_第2页

Citizen science and analytical chemistry. Oxymoron or harmony of meaning? 公民科学和分析化学。矛盾修饰法还是意思和谐？

IF 3.2

Journal of chromatography open Pub Date : 2026-05-01 Epub Date: 2025-12-20 DOI: 10.1016/j.jcoa.2025.100302

Giacomo Musile , Miryam Perrucci , Erika Maria Ricci , Antonio Maria Catena , Cristian D’Ovidio , Marcello Locatelli

{"title":"Citizen science and analytical chemistry. Oxymoron or harmony of meaning?","authors":"Giacomo Musile , Miryam Perrucci , Erika Maria Ricci , Antonio Maria Catena , Cristian D’Ovidio , Marcello Locatelli","doi":"10.1016/j.jcoa.2025.100302","DOIUrl":"10.1016/j.jcoa.2025.100302","url":null,"abstract":"<div><div>Citizen science is rapidly emerging as a transformative force in analytical chemistry by opening research participation to non-specialists and expanding where and how data can be collected. This report explores how recent technological advances have made analytical tools simpler, smaller, and more affordable. These developments have enabled citizens and students to be engaged directly in environmental monitoring, food-quality assessment, and educational activities, often producing data comparable to those generated in laboratories. In addition, several emerging prototypes have been purposefully designed with citizen use in mind, anticipating future applications in participatory science. The discussion also addresses areas where citizen science could play a growing role, including biomedical and forensic analysis, while recognizing challenges related to data reliability, ethics, and validation. Overall, this report highlights how accessible technologies, supported by artificial intelligence and digital communication, are transforming analytical chemistry into a more inclusive and collaborative discipline, connecting scientific research with everyday life.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100302"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145924369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Monitoring of morphine in urine after consumption of opium poppy sprouts using immunoassay and liquid chromatography coupled with quadrupole exactive Orbitrap mass spectrometry 利用免疫分析和液相色谱-四极杆精确轨道阱质谱联用技术监测罂粟芽食用后尿液中的吗啡

IF 3.2

Journal of chromatography open Pub Date : 2026-05-01 Epub Date: 2026-02-15 DOI: 10.1016/j.jcoa.2026.100314

Duygu Yeşim Ovat , Emine Özbunar , Özlem Akın , İnci Sağlam , Serap Annette Akgür

{"title":"Monitoring of morphine in urine after consumption of opium poppy sprouts using immunoassay and liquid chromatography coupled with quadrupole exactive Orbitrap mass spectrometry","authors":"Duygu Yeşim Ovat , Emine Özbunar , Özlem Akın , İnci Sağlam , Serap Annette Akgür","doi":"10.1016/j.jcoa.2026.100314","DOIUrl":"10.1016/j.jcoa.2026.100314","url":null,"abstract":"<div><div>Opiate use remains a growing global concern, particularly in the context of drug testing and forensic toxicology, where the differentiation between illicit drug use and dietary exposure continues to pose a significant interpretative challenge. Poppy seed–based products on opiate drug test results have been extensively investigated, although the impact of consuming fresh opium poppy sprouts, locally known as “afiyan,” hasn’t previously been evaluated. This study aimed to investigate whether the consumption of fresh poppy sprouts could result in presumptive positive opiate results and to assess the implications for forensic toxicological interpretation.Ten healthy volunteers who regularly consumed this herb were included in the study. Urine samples were collected over a 48 h period under non-fasting conditions. Opiate screening was performed using immunoassay at two cut-off concentrations (300 and 2000ng/mL). Quantitative analysis of morphine, codeine, thebaine, norcodeine, and dihydrocodeine was conducted using a validated Orbitrap LC–MS/MS method (linearity:30–1200 ng/mL; LLOQ: 30ng/mL for all analytes). At the 300ng/mL screening cut-off, 70% (<em>n</em> = 7) of participants yielded positive results within the first hours following ingestion. Morphine concentrations exceeded the LLOQ in first-hour urine samples from nine participants and persisted for up to 12 h in eight participants (37.95–150.23ng/mL). Morphine was still detected after 24 h in five participants (44.38–113.4ng/mL). These findings demonstrate that consumption of fresh poppy sprouts can lead to detectable urinary morphine concentrations capable of triggering positive opiate screening results. This work provides novel analytical evidence on the alkaloid profile and forensic relevance of fresh poppy sprout consumption.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100314"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147384194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estimating non-specific aquatic toxicity for the fathead minnow using surrogate chromatographic models 用替代色谱模型估计黑头鲦鱼的非特异性水生毒性

IF 3.2

Journal of chromatography open Pub Date : 2026-05-01 Epub Date: 2026-01-15 DOI: 10.1016/j.jcoa.2026.100307

Sanka N. Atapattu , Colin F. Poole

{"title":"Estimating non-specific aquatic toxicity for the fathead minnow using surrogate chromatographic models","authors":"Sanka N. Atapattu , Colin F. Poole","doi":"10.1016/j.jcoa.2026.100307","DOIUrl":"10.1016/j.jcoa.2026.100307","url":null,"abstract":"<div><div>In the assessment of aquatic toxicity, the fathead minnow (<em>Pimephales promelas</em>), a small fish species, is commonly used as an indicator. A major challenge in environmental risk assessment is predicting the aquatic toxicity of tens of thousands of emerging chemical contaminants and their metabolites, especially when no experimental data exist in the public domain or there is no means to generate new information. In this work the correlation of reversed-phase liquid chromatography and micellar electrokinetic chromatography retention factors to estimate fathead minnow toxicity was explored. We identified several surrogate chromatographic systems to estimate aquatic non-specific toxicity for the fathead minnow. We identified the reversed-phase liquid chromatography system comprising the XTerra MS C<sub>18</sub> 10% tetrahydrofuran-water binary solvent system and the micellar electrokinetic chromatography system utilizing the surfactant hexadecyltrimethylammonium bromide with prediction errors of 0.231–0.287 and 0.141–0.179 log units as suitable surrogate chromatographic systems to estimate non-specific aquatic toxicity for the fathead minnow.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100307"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146022476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stability issue of amino acids and organic acids in exhaled breath condensate and the potential use for lung cancer diagnosis 呼出液中氨基酸和有机酸的稳定性问题及其在肺癌诊断中的潜在应用

IF 3.2

Journal of chromatography open Pub Date : 2026-05-01 Epub Date: 2026-04-21 DOI: 10.1016/j.jcoa.2026.100338

Korcan Korba , Ebru Calkan Yildirim , Su Ozgur , Tuncay Goksel , Fatma Nil Ertas , Levent Pelit

{"title":"Stability issue of amino acids and organic acids in exhaled breath condensate and the potential use for lung cancer diagnosis","authors":"Korcan Korba , Ebru Calkan Yildirim , Su Ozgur , Tuncay Goksel , Fatma Nil Ertas , Levent Pelit","doi":"10.1016/j.jcoa.2026.100338","DOIUrl":"10.1016/j.jcoa.2026.100338","url":null,"abstract":"<div><div>As a non-invasive biofluid, exhaled breath condensate (EBC), which contains various volatile and non-volatile metabolites, such as amino acids (AAs) and organic acids (OAs), offers immense potential for metabolic monitoring and disease diagnosis. Although the ubiquitous nature of AAs and OAs in overlapping biochemical networks often complicates their use as standalone biomarkers, profiling these central metabolites remains crucial for understanding disease-induced metabolic dysregulation. Despite their proposed biomarker properties in conventional matrices such as blood and urine, the investigation of these metabolites in EBC is remarkably sparse, highlighting a clear imperative for further research. The objective of this study is to evaluate the impact of storage stability conditions of selected AAs and OAs in EBC, optimize their analytical conditions, and investigate their potential as non-invasive biomarkers for lung cancer (LuC) diagnosis. After revealing the storage conditions, a study was conducted in which EBC samples were collected from 30 patients diagnosed with LuC and 19 healthy subjects. While five metabolites (pipecolic acid, aspartic acid, taurine, succinic acid, and trigonelline), have been detected in all samples, notable prevalence variations emerged: phenylalanine was elevated in LuC patients (83% vs. 53%), whereas tryptophan and threonine exhibited rare, group-exclusive distributions. Conversely, the AA tryptophan was found exclusively in two healthy individuals and was absent in all LuC patient samples. Although statistical evaluations, including the Mann-Whitney U test and AUC-ROC analysis, revealed that the dataset lacks absolute discriminative power, these foundational EBC profiles provide critical insights for advancing future non-invasive diagnostic studies.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100338"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147849544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A critical review and analysis of thermodynamic and kinetic effects on extraction of medical devices by organic solvents as a sample preparation step in devices’ chemical characterization 综述和分析了有机溶剂萃取医疗器械作为样品制备步骤在器械化学表征中的热力学和动力学影响

IF 3.2

Journal of chromatography open Pub Date : 2026-05-01 Epub Date: 2026-01-18 DOI: 10.1016/j.jcoa.2026.100309

By Jianwei Li

{"title":"A critical review and analysis of thermodynamic and kinetic effects on extraction of medical devices by organic solvents as a sample preparation step in devices’ chemical characterization","authors":"By Jianwei Li","doi":"10.1016/j.jcoa.2026.100309","DOIUrl":"10.1016/j.jcoa.2026.100309","url":null,"abstract":"<div><div>The extraction of medical devices by organic solvents as a sample preparation step is perhaps the most critical process in chemical characterization of medical devices, as the decision of solvent choice determines not only the resulting extractables profiles (e.g., the number, molecular weight, and concentration of extractables), but also the degree of analytical challenges in identification and quantification, when performing instrumental analysis of extracts. Both concentration and chemical identities of all extractables are used in subsequent toxicological risk assessment, which is required to support regulatory submission and approval of medical devices. There is currently a considerable debate on which solvent(s) should be used to balance the desired extraction and clinical relevance to biological tissue exposure due to scientific and regulatory consequences. The purpose of this article is intended to streamline solvent selection in chemical characterization, particularly on the rational development of a semipolar solvent to stimulate or exaggerate biological (storage) fatty tissues. A comprehensive review and analysis of solvent extraction of polymer materials from thermodynamic and kinetic perspectives is provided first. The solvent-material interactions (swelling) and their consequences on kinetics and thermodynamics are subsequently elaborated. The conclusions of thermodynamic and kinetic properties, together with solvent-material interactions, are used to establish solvent similarity criteria between semipolar solvents and fatty tissues. Finally, solvent solubility, degree of material swelling by solvents, diffusion coefficients in device material, partition coefficient between material and solvent, and solvent compatibility with instrumental analysis are used as the criteria to establish representative solvents to fatty tissues in chemical characterization studies. Blood simulating solvents are well established and not discussed in the article.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100309"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146023058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The challenges and prospects of capillary zone electrophoresis-mass spectrometry for characterization of monoclonal antibodies biotherapeutics 毛细管区带电泳-质谱技术用于单克隆抗体生物治疗药物鉴定的挑战与展望

IF 3.2

Journal of chromatography open Pub Date : 2026-05-01 Epub Date: 2025-11-25 DOI: 10.1016/j.jcoa.2025.100293

Claudia Desiderio , Alexandra Muntiu

{"title":"The challenges and prospects of capillary zone electrophoresis-mass spectrometry for characterization of monoclonal antibodies biotherapeutics","authors":"Claudia Desiderio , Alexandra Muntiu","doi":"10.1016/j.jcoa.2025.100293","DOIUrl":"10.1016/j.jcoa.2025.100293","url":null,"abstract":"<div><div>The application of Capillary Zone Electrophoresis (CZE) in proteomic analysis has increased significantly since the technique was hyphenated with high resolution mass spectrometry (HR-MS). The coupling was successful in combining the analytical features of high separation efficiency and resolving power of CZE with the precision of molecular mass measurements and sequencing of HR-MS analyzers for characterization of proteins and peptides and of their proteoforms, even in pre-digested, intact and native form. In this regard, CZE has made a strong contribution in the field of analysis and characterization of monoclonal antibodies (mAb) biotherapeutics, due to its high resolution power and separation mechanism based on differential migration, demonstrating challenging features for discriminating variants with rapid and high-efficiency analyses and for assessment of manufacturing quality control and safety of the final product. This review aims to highlight the most recent applications of CZE-MS in separation and characterization of mAb biotherapeutics based on the most recent literature (2022-2025). The various analytical approaches used in proteins MS characterization will be illustrated, with particular emphasis on the top-down approach, widely applied in this field of analysis and offering comprehensive characterization of proteoforms and exploration of protein interactions under native conditions. CZE-MS demonstrated an effective analytical strategy for the detailed characterization of mAb proteoforms with high potential for application in the challenging task of monitoring mAb release in advanced drug delivery systems.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100293"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145618376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modified bi-Gaussian models to describe chromatographic peaks as a function of peak-shape parameters 改进双高斯模型，将色谱峰描述为峰形参数的函数

IF 3.2

Journal of chromatography open Pub Date : 2026-05-01 Epub Date: 2026-02-21 DOI: 10.1016/j.jcoa.2026.100317

J.J. Baeza-Baeza, L.J. Baeza-Ballesteros, J.R. Torres-Lapasió, M.C. García-Alvarez-Coque

{"title":"Modified bi-Gaussian models to describe chromatographic peaks as a function of peak-shape parameters","authors":"J.J. Baeza-Baeza, L.J. Baeza-Ballesteros, J.R. Torres-Lapasió, M.C. García-Alvarez-Coque","doi":"10.1016/j.jcoa.2026.100317","DOIUrl":"10.1016/j.jcoa.2026.100317","url":null,"abstract":"<div><div>The modelling and prediction of chromatographic peak profiles have been extensively studied, with foundational contributions such as Grushka's bi-Gaussian model. In this work, this approach is extended to two modified Gaussian models: The Linear Modified Gaussian (LMG) and the Parabolic Variance Modified Gaussian (PVMG). These models enhance the traditional Gaussian framework by allowing the peak variance to change according to parabolic trends, thereby capturing deviations from ideal Gaussian peak shapes. This added flexibility enables a more accurate representation of a wide range of chromatographic peak shapes with varying degrees of asymmetry. The proposed modified bi-Gaussian models (BLMG and BVMG with six and seven parameters) use peak-shape parameters that can be expressed as functions of retention time. This makes them well suited for predicting peak shape and chromatographic resolution, which are key factors in optimising the separation of complex analyte mixtures. The BVMG model with seven parameters yields fitting errors below 1 % for most peaks and below 2 % for the peaks of all assayed probe compounds, comparable to the more sophisticated Parabolic Lorentzian Modified Gaussian (PLMG) model, while relying only on peak-shape parameters (retention time, peak height at the peak maximum, and half-widths measured at 60 and 10 % of peak height). The performance of the models is validated through the fitting and prediction of chromatographic peaks for compounds eluted using two columns with distinct retention mechanisms: Zorbax C18 and Hypercarb porous graphitic carbon columns.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100317"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147384201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyphenolic profiling of Euphorbia cardiophylla, an endemic Turkish species, using Liquid chromatography-High resolution Orbitrap mass spectrometry and investigating its biological activity 用液相色谱-高分辨率轨道阱质谱法分析土耳其特有植物大鼠心叶的多酚类物质，并研究其生物活性

IF 3.2

Journal of chromatography open Pub Date : 2026-05-01 Epub Date: 2026-02-12 DOI: 10.1016/j.jcoa.2026.100312

Inan Dursun , Lütfi Behçet , Cemil Aydoğan

{"title":"Polyphenolic profiling of Euphorbia cardiophylla, an endemic Turkish species, using Liquid chromatography-High resolution Orbitrap mass spectrometry and investigating its biological activity","authors":"Inan Dursun , Lütfi Behçet , Cemil Aydoğan","doi":"10.1016/j.jcoa.2026.100312","DOIUrl":"10.1016/j.jcoa.2026.100312","url":null,"abstract":"<div><div>This study used<img> LC/High resolution Orbitrap-mass spectrometry (LC/HR Orbitrap-MS) to comprehensively investigate the phenolic compound profile and biological activities of <em>E. cardiophylla</em> a species endemic to Türkiye. The leaf and root methanol extracts of the plant were evaluated, and their total phenolic (TPC) and total flavonoid (TFC) contents were determined using various antioxidant tests (DPPH, ABTS and CUPRAC). The findings revealed that the leaf extract contained significantly higher levels of phenolic compounds than the root extract, with TPC and TFC values of 125.64 ± 6.25 mg GAE/g and 49.42 ± 1.89 mg QE/g, respectively. Antioxidant activity tests showed that the leaf extract had much higher capacities for DPPH (309.04 ± 2.64 mg TE/g), ABTS (481.11 ± 9.12 mg TE/g) and CUPRAC (787.86 ± 15.09 mg TE/g) than the root extract. Analysis using LC/HR Orbitrap-MS <img>has revealed the presence of compounds such as rutin hydrate (19,351.87 µg/g), quinic acid (31,473.66 µg/g), isoquercitrin (11,546.45 µg/g), astragalin (5472.01 µg/g) and gallic acid (1459.39 µg/g) at high levels in the <em>E. cardiophylla</em> plant. These results demonstrate that <em>E. cardiophylla</em> has a high phenolic content and strong antioxidant properties, suggesting that it could be a valuable source of natural bioactives for use in pharmaceuticals and nutraceuticals.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100312"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147384701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial — Multidimensional chromatography: When complexity demands complementarity 编辑-多维色谱：当复杂性需要互补性时

IF 3.2

Journal of chromatography open Pub Date : 2026-05-01 Epub Date: 2026-03-20 DOI: 10.1016/j.jcoa.2026.100326

Giorgia Purcaro

引用次数: 0

Measurement of sesamin and asarinin in the roots, flowers, leaves and stems of Anemopsis californica by high-performance liquid chromatography 高效液相色谱法测定加州风参根、花、叶和茎中芝麻素和细辛素的含量

IF 3.2

Journal of chromatography open Pub Date : 2026-05-01 Epub Date: 2026-05-20 DOI: 10.1016/j.jcoa.2026.100346

Angie A. Quintero-Guerrero , Dalia I. Sánchez-Machado , Jaime López-Cervantes , Olga N. Campas-Baypoli , María dR. Martínez-Macias , Karen L. Hernández-Ruiz , Andrés F. Chávez-Almanza

{"title":"Measurement of sesamin and asarinin in the roots, flowers, leaves and stems of Anemopsis californica by high-performance liquid chromatography","authors":"Angie A. Quintero-Guerrero , Dalia I. Sánchez-Machado , Jaime López-Cervantes , Olga N. Campas-Baypoli , María dR. Martínez-Macias , Karen L. Hernández-Ruiz , Andrés F. Chávez-Almanza","doi":"10.1016/j.jcoa.2026.100346","DOIUrl":"10.1016/j.jcoa.2026.100346","url":null,"abstract":"<div><div><em>Anemopsis californica</em> is a medicinal plant used by indigenous ethnic groups in northwest Mexico and the southwestern United States. Among its chemical components are the lignans sesamin and asarinin, which have important biological functions. This study describes a liquid chromatography method for the simultaneous quantification of sesamin and asarinin in the roots, flowers, leaves and stems of this plant.</div><div>In the present work, the method intra- and inter-day precision (reported as RSD %; n = 8 for intra-day and n = 3 for inter-day, corresponding to three consecutive weeks, with four replicate measurements per week) for sesamin were between 2.37 and 4.57 %, and those for asarinin were between 2.53 and 5.37 %, respectively. The recovery of sesamin and asarinin in roots, flowers, leaves, and stems ranged from 92.40 to 98.80 % and from 91.20 to 99.87 %, respectively. When comparing the contents of these compounds during different flowering times, it was found that the contents of both lignans were greater (almost double) when the plant was harvested in summer than when the plant was harvested in spring. The roots were the main sources of sesamin and asarinin; the average dry sample content of sesamin in the summer roots was 845.55 µg/g dry sample, and that of asarinin was 1701.51 µg/g dry sample. This study proposes a reliable HPLC method for the quantitative determination of both lignans in all parts of <em>Anemopsis californica</em>, highlighting its potential medicinal relevance and supporting future research on this species.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100346"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148167356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0