Journal of chromatography open最新文献

{"title":"Determination of chemical components, phytochemical (polyphenol) profile, microorganisms and mycotoxins and evaluation of antimicrobial potential of Pimpinella anisum (Anise) and Foeniculum vulgare (Fennel) against bacteria isolated from cats","authors":"Marijana Sokolovic ,&nbsp;Natasa P. Kalogiouri ,&nbsp;Victoria F. Samanidou ,&nbsp;Camelia Tulcan ,&nbsp;Camen Dorin ,&nbsp;Roberta Tripon ,&nbsp;Snježana Kazazić ,&nbsp;Fani Krstulović ,&nbsp;Mirta Balenović ,&nbsp;Tajana Amšel Zelenika ,&nbsp;Ana Vulić ,&nbsp;Claudia Daniela Serban ,&nbsp;Marija Berendika","doi":"10.1016/j.jcoa.2025.100275","DOIUrl":"10.1016/j.jcoa.2025.100275","url":null,"abstract":"<div><div>Nutritional, microbiological and toxicological quality of plants like <em>Pimpinella anisum</em> (anise) and <em>Foeniculum vulgare</em> Mill (fennel) are important factors in assuring their beneficial characteristics and usage. Besides defining basic nutritional and safety status, understanding the profile of compounds like polyphenols provides insights into their potential phytochemical, antioxidant and antimicrobial effects. The aim of this study was to evaluate chemical composition and polyphenol profile, mycotoxicological and microbiological quality and assess the antimicrobial potential of fennel extracts against bacterial pathogens isolated from cats.</div><div>In our study, the proximate chemical compositions of both seeds were in line with previous researches. In fennel and anise seeds, the most abundant group of fatty acids were monosaturated fatty acids (82.15%, 67.16%), followed by polysaturated fatty acids (10.81%, 23.96%) and saturated fatty acids (7.04%, 8.88%). Among individual fatty acids, the most abundant were oleic acid (81.02%, 65.05%), followed by linoleic acid (10.29%, 23.06%), respectively. Polyphenol profiling of plant extracts showed the highest amounts of Hydroxytyrosol (1069.0 mg/kg), Naringenin (420.3 mg/kg) and Epicatechin G (555.4 mg/kg) in fennel, while Naringenin (667.3 mg/kg), Apigenin 360.4 mg/kg), Luteolin (360.4 mg/kg), Coutaric Acid (328.9 mg/kg) and Caftaric acid (317.7 mg/kg) were detected in anise. Microbiological analysis of both plants showed presence of bacteria, fungi, yeasts and tested mycotoxins. Antimicrobial activity of both plants was demonstrated on wild bacterial isolates from cats, with noticed differences for tested extraction solvents. The results of our study show the promising potential of plant extracts for treatment of infections caused by tested pathogenic bacteria.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100275"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145537589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a simultaneous pesticide analysis method for herbal medicines using a modified extraction procedure and ultrahigh-performance liquid chromatography tandem mass spectrometry","authors":"Yeojin Yoon,&nbsp;Eunseong Jo,&nbsp;Jinchul Kim,&nbsp;Yong Beom Cho,&nbsp;Kyu-Heon Kim,&nbsp;Ji-Young Park,&nbsp;Seong Mi Lee,&nbsp;InYeong Hwang,&nbsp;Jonghwan Kim,&nbsp;Jinhee Hwang","doi":"10.1016/j.jcoa.2025.100281","DOIUrl":"10.1016/j.jcoa.2025.100281","url":null,"abstract":"<div><div>Owing to complex matrix interference and low analyte concentrations, pesticide residues in herbal medicines pose considerable health and environmental risks. In this study, a modified QuEChERS protocol combined with ultra-high-performance liquid chromatography–tandem mass spectrometry was developed for the simultaneous determination of 239 pesticides in six widely used herbal medicines. Extraction was performed using MeCN/EtOAc (7:3, v/v) acidified with 0.1 % formic acid to enhance the recovery of both acidic and neutral compounds, followed by dispersive SPE cleanup using 150 mg of MgSO₄ and 25 mg of C₁₈ to reduce matrix effects. Chromatographic separation was performed on a C₁₈ column using a gradient of water and methanol (both containing 5 mM ammonium formate and 0.1 % formic acid), and sensitive MRM detection was achieved using electrospray ionization with positive/negative polarity switching. Method validation was conducted using Paeoniae Radix, Cnidii Rhizoma, Rehmanniae Radix, Corni Fructus, Artemisiae Argyi Folium, and Carthami Flos, following the European Commission SANTE/2020/12,830 guidance. For over 90 % of pesticides, calibration curves exhibited R² &gt; 0.99, and recoveries ranged from 70 to 120 % with RSD &lt; 20 %. Thus, the proposed method is rapid, facile, reliable, and effective for analyzing pesticide residues in diverse herbal medicines.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100281"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145572016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On the effect of aqueous organic mobile phases on the enantioseparation of halogenated 4,4’-bipyridines using polysaccharide carbamate-based chiral columns","authors":"Barbara Sechi ,&nbsp;Giorgi Jibuti ,&nbsp;Roberto Dallocchio ,&nbsp;Sergio Cossu ,&nbsp;Bezhan Chankvetadze ,&nbsp;Victor Mamane ,&nbsp;Paola Peluso","doi":"10.1016/j.jcoa.2026.100319","DOIUrl":"10.1016/j.jcoa.2026.100319","url":null,"abstract":"<div><div>In the last decade, the contribution of halogen bond (HaB) in liquid phase enantioseparation of halogenated analytes was demonstrated by using polysaccharide-based chiral stationary phases (CSPs) and <em>n</em>-hexane-containing mixtures as mobile phases. On the other hand, it was also shown that polar organic solvents (POSs) like methanol and ethanol weaken or suppress the contribution of HaB to binding and recognition, whereas hydrophobic mechanisms become dominant by using alcohol-based mobile phases. Although some recent studies demonstrated that HaB-based systems exhibit an improved water resistance compared to the analogous hydrogen bond (HB)-based systems, currently no information is available in the literature about the effect of using aqueous organic mobile phases on the enantioseparations of halogenated compounds driven by HaB in normal phase mode. With the aim to gain information about this topic, we investigated the effect of adding water to the mobile phase on the enantioseparation of four hexahalogenated 4,4’-bipyridines by using amylose and cellulose <em>tris</em>(3,5-dimethylphenylcarbamate)-based chiral columns. As a result, chromatographic behaviours dependent on the distinctive halogen atom featuring the analytes were observed. The study confirmed that, when polar organic solvents and aqueous organic mixtures were used as mobile phase, both iodine-dependent and hydrophobic noncovalent interactions contributed to the enantioseparation of iodinated 4,4’-bipyridines.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100319"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147384193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromatography on monolithic supports for biological nanoparticles and large biomolecules: Recent developments and state-of-the-art","authors":"Tomislav Friganović ,&nbsp;Lucija Dončević ,&nbsp;Djuro Josić","doi":"10.1016/j.jcoa.2025.100300","DOIUrl":"10.1016/j.jcoa.2025.100300","url":null,"abstract":"<div><div>Chromatography on monolithic supports combines convective flow through interconnected macropores with tunable surface chemistries, enabling high-flow, low-shear purification of large biomolecules and biological nanoparticles. Recent developments in stationary-phase design, such as brush-grafted surfaces, together with optimized elution protocols, have additionally advanced both resolution and capacity across a broad range of targets. Demonstrated applications include the high-resolution separation of adeno-associated virus (AAV) empty and full capsids, isolation of supercoiled plasmid DNA, and removal of double-stranded RNA impurities from mRNA therapeutics. Furthermore, the platform’s tolerance for complex matrices enables efficient purification of extracellular vesicles and direct recovery of biotherapeutics from undiluted blood plasma. Miniaturized monolithic columns enable rapid and robust on-line monitoring of industrial processes for current good manufacturing practice (cGMP) control. Ongoing progress in multimodal chemistries, continuous and multicolumn operations, and integrated analytics establishes chromatography on monolithic supports as a versatile and robust platform for the rapid, selective purification of large biomolecules and biological nanoparticles and efficient monitoring of industrial processes.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100300"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145789690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving emulsification for pesticide control: The role of Phytolacca dodecandra leaf extract and tween 80","authors":"Meroda Tesfaye Gari ,&nbsp;Girma Gonfa ,&nbsp;Musin Kelel ,&nbsp;Belete Tessema","doi":"10.1016/j.jcoa.2026.100313","DOIUrl":"10.1016/j.jcoa.2026.100313","url":null,"abstract":"<div><div>This study focuses on optimizing the emulsification process utilizing <em>Phytolacca dodecandra</em> leaf extract in combination with emulsifier (Tween 80) for household cockroach control. The main aim is to evaluate and enhance emulsion stability and efficiency through systematic experimentation. Employing the Box-Behnken Design (BBD) within response surface methodology (RSM), key EC variables including mixing time (20, 40, and 60 min), mixing temperature (25, 42.5, and 60 °C), and crude extract with emulsifier ratio (10:10, 15:15, and 20:20 wt/wt) were investigated to determine optimization for the response variables of pH and viscosity of EC. The EC optimization, characterization, and efficiency were investigated. The results were demonstrated significant interactions among variables, with optimal emulsification achieved at mixing time (40 min), mixing temperature (42.5 °C), crude extract to Tween 80 ratio (15:15 wt/wt) with a pH (7.678) and viscosity (5.768 cp). At this optimal point, the UV–Vis spectroscopy (230–400 nm) confirmed the presence of bioactive compounds responsible for emulsification. FTIR analysis identified functional groups involved in stabilization the emulsion. By using natural plant extracts like <em>Phytolacca dodecandra</em> and surfactants like Tween 80, the research advances the development of sustainable and eco-friendly emulsification systems. Bioassay testing on cockroaches showed that the prepared emulsion at optimal result was effective; within ten minutes of exposure, a mortality rate of 100 % was observed using 5 ml of the EC for 15 cockroaches for three replicants. Whereas, a positive control (mortien) and negative control (Tween 80), were laid with three replications. Mortality 100 % and 0 % of cockroaches was recorded after 24 h of exposure for both positive and negative control respectively. This method provides an efficient and safe way to control household cockroaches, lowering the need for dangerous chemical pesticides and fostering safer living conditions.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100313"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147384185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimating the sorption of neutral organic compounds by soil from water using surrogate chromatographic models","authors":"Sanka N. Atapattu ,&nbsp;Azamat Temerdashev","doi":"10.1016/j.jcoa.2026.100308","DOIUrl":"10.1016/j.jcoa.2026.100308","url":null,"abstract":"<div><div>When assessing the fate and distribution of chemicals, the soil-water sorption is one of the most important physicochemical properties considered in environmental monitoring applications, particularly for emerging environmental contaminants. Since the experimental determination of the soil-water sorption coefficient (<em>K</em>oc) is time-consuming, tedious, and expensive, various estimation methods are often sought after. The solvation parameter model is a widely used linear free energy relationship model for estimating environmental and biophysical properties. In this work, we screened solvation parameter model system constants of reversed-phase liquid chromatography and micellar electrokinetic chromatography systems to identify surrogate chromatographic systems to estimate the sorption of neutral organic compounds by soil from water. The Ascentis C₁₈ stationary phase having the mobile phase system of methanol-water (1:1) with a prediction error for log <em>K</em>oc of 0.15 to 0.20 log units and the micellar electrokinetic chromatography system comprising 50 mM sodium dodecyl sulfate, 20 mM sodium phosphate-sodium tetraborate buffer at pH = 8 with a prediction error for log <em>K</em>oc of 0.11 to 0.15 log units are suitable surrogate systems to estimate soil-water sorption of neutral compounds.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100308"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147384434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimating non-specific aquatic toxicity for the fathead minnow using surrogate chromatographic models","authors":"Sanka N. Atapattu ,&nbsp;Colin F. Poole","doi":"10.1016/j.jcoa.2026.100307","DOIUrl":"10.1016/j.jcoa.2026.100307","url":null,"abstract":"<div><div>In the assessment of aquatic toxicity, the fathead minnow (<em>Pimephales promelas</em>), a small fish species, is commonly used as an indicator. A major challenge in environmental risk assessment is predicting the aquatic toxicity of tens of thousands of emerging chemical contaminants and their metabolites, especially when no experimental data exist in the public domain or there is no means to generate new information. In this work the correlation of reversed-phase liquid chromatography and micellar electrokinetic chromatography retention factors to estimate fathead minnow toxicity was explored. We identified several surrogate chromatographic systems to estimate aquatic non-specific toxicity for the fathead minnow. We identified the reversed-phase liquid chromatography system comprising the XTerra MS C₁₈ 10% tetrahydrofuran-water binary solvent system and the micellar electrokinetic chromatography system utilizing the surfactant hexadecyltrimethylammonium bromide with prediction errors of 0.231–0.287 and 0.141–0.179 log units as suitable surrogate chromatographic systems to estimate non-specific aquatic toxicity for the fathead minnow.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100307"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146022476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Citizen science and analytical chemistry. Oxymoron or harmony of meaning?","authors":"Giacomo Musile ,&nbsp;Miryam Perrucci ,&nbsp;Erika Maria Ricci ,&nbsp;Antonio Maria Catena ,&nbsp;Cristian D’Ovidio ,&nbsp;Marcello Locatelli","doi":"10.1016/j.jcoa.2025.100302","DOIUrl":"10.1016/j.jcoa.2025.100302","url":null,"abstract":"<div><div>Citizen science is rapidly emerging as a transformative force in analytical chemistry by opening research participation to non-specialists and expanding where and how data can be collected. This report explores how recent technological advances have made analytical tools simpler, smaller, and more affordable. These developments have enabled citizens and students to be engaged directly in environmental monitoring, food-quality assessment, and educational activities, often producing data comparable to those generated in laboratories. In addition, several emerging prototypes have been purposefully designed with citizen use in mind, anticipating future applications in participatory science. The discussion also addresses areas where citizen science could play a growing role, including biomedical and forensic analysis, while recognizing challenges related to data reliability, ethics, and validation. Overall, this report highlights how accessible technologies, supported by artificial intelligence and digital communication, are transforming analytical chemistry into a more inclusive and collaborative discipline, connecting scientific research with everyday life.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100302"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145924369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring of morphine in urine after consumption of opium poppy sprouts using immunoassay and liquid chromatography coupled with quadrupole exactive Orbitrap mass spectrometry","authors":"Duygu Yeşim Ovat ,&nbsp;Emine Özbunar ,&nbsp;Özlem Akın ,&nbsp;İnci Sağlam ,&nbsp;Serap Annette Akgür","doi":"10.1016/j.jcoa.2026.100314","DOIUrl":"10.1016/j.jcoa.2026.100314","url":null,"abstract":"<div><div>Opiate use remains a growing global concern, particularly in the context of drug testing and forensic toxicology, where the differentiation between illicit drug use and dietary exposure continues to pose a significant interpretative challenge. Poppy seed–based products on opiate drug test results have been extensively investigated, although the impact of consuming fresh opium poppy sprouts, locally known as “afiyan,” hasn’t previously been evaluated. This study aimed to investigate whether the consumption of fresh poppy sprouts could result in presumptive positive opiate results and to assess the implications for forensic toxicological interpretation.Ten healthy volunteers who regularly consumed this herb were included in the study. Urine samples were collected over a 48 h period under non-fasting conditions. Opiate screening was performed using immunoassay at two cut-off concentrations (300 and 2000ng/mL). Quantitative analysis of morphine, codeine, thebaine, norcodeine, and dihydrocodeine was conducted using a validated Orbitrap LC–MS/MS method (linearity:30–1200 ng/mL; LLOQ: 30ng/mL for all analytes). At the 300ng/mL screening cut-off, 70% (<em>n</em> = 7) of participants yielded positive results within the first hours following ingestion. Morphine concentrations exceeded the LLOQ in first-hour urine samples from nine participants and persisted for up to 12 h in eight participants (37.95–150.23ng/mL). Morphine was still detected after 24 h in five participants (44.38–113.4ng/mL). These findings demonstrate that consumption of fresh poppy sprouts can lead to detectable urinary morphine concentrations capable of triggering positive opiate screening results. This work provides novel analytical evidence on the alkaloid profile and forensic relevance of fresh poppy sprout consumption.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100314"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147384194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochar-based “in-vial” stir bar sorptive microextraction for a greener determination of steroid hormones in saliva","authors":"Valentina Quarta,&nbsp;Francesca Merlo,&nbsp;Chiara Milanese,&nbsp;Antonella Profumo,&nbsp;Andrea Speltini","doi":"10.1016/j.jcoa.2025.100287","DOIUrl":"10.1016/j.jcoa.2025.100287","url":null,"abstract":"<div><div>This work presents a novel application of biochar as a new-generation sorbent to bioanalytical sample preparation. The carbonaceous material produced by pyrolysis of vegetal wastes (orange and pumpkin peels) at 650°C was easily attached (∼ 4 mg) to a small magnetic stir bar (8 × 3 mm), enabling a novel stir bar sorptive microextraction protocol to be performed directly in the HPLC-MS vial. The device was first tested in synthetic saliva samples (1 mL) observing sorption equilibrium in 1 h contact time and full elution was achieved using 300 µL 1 % v/v acetic acid ethanol solution after 30 min. Under the selected conditions, recovery in real saliva, spiked with each analyte in the range 0.5-5 ng mL^-1, was in the range 88%-112%, with good inter-day inter-batch device-to-device precision (RSD &lt; 18%, <em>n=3</em>), and no need for additional clean-up. Reusability tests confirmed that the device retains its sorption/desorption efficiency over at least 20 consecutive cycles. Additionally, the greenness of this biochar-based “in-vial” approach as a new stir bar sorptive microextraction was proven by using different dedicated software. The practical applicability of the procedure was demonstrated in real saliva samples from healthy volunteers.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"9 ","pages":"Article 100287"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145572015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}