Bulletin of Russian State Medical University最新文献_第9页

Androgen levels in blood and follicular fluid of IVF patients with diminished ovarian reserve 卵巢储备功能减退的体外受精患者血液和卵泡液中的雄激素水平

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Bulletin of Russian State Medical University Pub Date : 2022-08-01 DOI: 10.24075/brsmu.2022.041

A. Gavisova, M. Shevtsova, SV Kindysheva, N. Starodubtseva, V. Frankevich, T. Nazarenko, N. Dolgushina

引用次数: 0

Content of CD4+ cells expressing CD39/CD73 ectonucleotidases in children with inflammatory bowel diseases 炎症性肠病患儿表达CD39/CD73外核苷酶的CD4+细胞含量

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Bulletin of Russian State Medical University Pub Date : 2022-07-01 DOI: 10.24075/brsmu.2022.039

T. Radygina, S. Petrichuk, D. Kuptsova, A. Potapov, AS Illarionov, AO Anushenko, O. Kurbatova, E. Semikina

{"title":"Content of CD4+ cells expressing CD39/CD73 ectonucleotidases in children with inflammatory bowel diseases","authors":"T. Radygina, S. Petrichuk, D. Kuptsova, A. Potapov, AS Illarionov, AO Anushenko, O. Kurbatova, E. Semikina","doi":"10.24075/brsmu.2022.039","DOIUrl":"https://doi.org/10.24075/brsmu.2022.039","url":null,"abstract":"The regulation of TNF inhibitor therapy-associated immune responses in inflammatory bowel diseases (IBD) in children remains an urgent problem. The study aimed at analyzing the expression of CD39/CD73 endonucleotidases by different subsets of peripheral blood T cells in children with IBD including Crohn's disease (n = 34) and ulcerative colitis (n = 33) having received TNF inhibitors in comparison with conditionally healthy children (n = 45). Lymphocyte subsets including regulatory T cells (Treg, CD4+CD127lowCD25high), activated T cells (Tact, CD4+CD25+CD127high) and Th17 cells (CD4+CD161+CD3+) were studied by flow cytometry. The results are presented as medians (Me) and quartiles (Q25–Q75). In children with IBD the highest and the lowest relative counts of CD39+ cells were found in Treg and Tact subsets — 31% (15–38) and 4% (1–7), respectively. The highest relative counts of CD73+ cells were found in Tact — 13% (8–21). The CD39 and CD73 expression ratio in patients with IBD, and in the control group as well, depended on particular subset. CD39 expression in Treg, Tact and Th17 of patients with IBD was not age-dependent. Patients with acute Crohn's disease revealed decreased expression of CD39 in Treg compared with the control group (12% (9–23) vs 35% (28–39), respectively; р = 10–6). Patients with Crohn's disease in remission revealed increased expression of CD39 in Treg compared with the acute of the disease (31% (27–40) vs 12% (9–23); р = 9.4 × 10–5). Patients with Crohn's disease in remission revealed no significant differences with the control group apart from reduced expression of CD73 by Treg in Crohn's disease. The results indicate significant association of CD39 and CD73 expression levels in particular subsets of CD4+ cells with the phase of the disease (acute vs remission) and, accordingly, with the anti-TNF regimen efficacy.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":"1 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68987105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in diagnostics of neonatal hypoxic ischemic encephalopathy 新生儿缺氧缺血性脑病诊断的最新进展

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Bulletin of Russian State Medical University Pub Date : 2022-07-01 DOI: 10.24075/brsmu.2022.038

N. Starodubtseva, ChM Eldarov, A. Kirtbaya, E. Balashova, A.S. Gryzunova, O.I. Ionov, VV Zubkov, DN Silachev

{"title":"Recent advances in diagnostics of neonatal hypoxic ischemic encephalopathy","authors":"N. Starodubtseva, ChM Eldarov, A. Kirtbaya, E. Balashova, A.S. Gryzunova, O.I. Ionov, VV Zubkov, DN Silachev","doi":"10.24075/brsmu.2022.038","DOIUrl":"https://doi.org/10.24075/brsmu.2022.038","url":null,"abstract":"The prognosis in neonatal hypoxic ischemic encephalopathy (HIE) depends on early differential diagnosis for justified administration of emergency therapeutic hypothermia. The moment of therapy initiation directly affects the long-term neurological outcome: the earlier the commencement, the better the prognosis. This review analyzes recent advances in systems biology that facilitate early differential diagnosis of HIE as a pivotal complement to clinical indicators. We discuss the possibilities of clinical translation for proteomic, metabolomic and extracellular vesicle patterns characteristic of HIE and correlations with severity and prognosis. Identification and use of selective biomarkers of brain damage in neonates during the first hours of life is hindered by systemic effects of hypoxia. Chromatography– mass spectrometry blood tests allow analyzing hundreds and thousands of metabolites in a small biological sample to identify characteristic signatures of brain damage. Clinical use of advanced analytical techniques will facilitate the accurate and timely diagnosis of HIE for enhanced management.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45415649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

In silico algorithm for optimization of pharmacokinetic studies of [25Mg2+]porphyrin-fullerene nanoparticles [25Mg2+]卟啉-富勒烯纳米颗粒药代动力学研究的计算机算法优化

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Bulletin of Russian State Medical University Pub Date : 2022-07-01 DOI: 10.24075/brsmu.2022.037

V. Fursov, DI Zinchenko, DD Namestnikova, DA Kuznetsov

引用次数: 0

Comparative efficiency of accessible transfection methods in model cell lines for biotechnological applications 用于生物技术应用的模型细胞系中可接近转染方法的比较效率

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Bulletin of Russian State Medical University Pub Date : 2022-06-01 DOI: 10.24075/brsmu.2022.031

P. Vorobyev, DV Kochetkov, K. Vasilenko, A. Lipatova

{"title":"Comparative efficiency of accessible transfection methods in model cell lines for biotechnological applications","authors":"P. Vorobyev, DV Kochetkov, K. Vasilenko, A. Lipatova","doi":"10.24075/brsmu.2022.031","DOIUrl":"https://doi.org/10.24075/brsmu.2022.031","url":null,"abstract":"Transient gene expression is one of the most common methods in molecular biology, equally relevant for basic research projects and biotechnological industries. Despite the existence of commercial transfection systems, which afford high transfection efficiency and high expression levels of reporter genes, expanding such systems to industrial scales is often problematic due to high costs of the reagents. The well-described methods of cationic and calcium-phosphate transfection are accessible and ensure reproducible results at much lower costs. This study is aimed at comparative validation of calcium phosphate and cationic (polyethylenimine-based) transfection protocols along with the commercially available TurboFect reagent for mono- and cotransfections on a panel of commonly used cell lines including HEK293T, Huh7, BHK-21, CHO and MRC5. The efficiency of transfection with plasmid constructs encoding different fluorescent proteins was measured by flow cytometry. Of all the tested methods, calcium phosphate transfection afforded the highest efficiency of plasmid DNA delivery in all the cell lines except BHK21, for which the PEI method turned out to be more efficient than calcium phosphate transfection, and CHO, for which both methods showed comparable efficiency.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41620783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical and morphological features of non-small cell lung cancer in patients with different types of histological changes to the bronchial epithelium 不同类型支气管上皮组织学改变的非小细胞肺癌患者的临床形态学特征

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Bulletin of Russian State Medical University Pub Date : 2022-06-01 DOI: 10.24075/brsmu.2022.030

M. Zavyalova, D. Loos, DS Pismenny, A. Durova, O. Pankova, E. Rodionov, SA Tuzikov, L. Tashireva, V. Perelmuter

{"title":"Clinical and morphological features of non-small cell lung cancer in patients with different types of histological changes to the bronchial epithelium","authors":"M. Zavyalova, D. Loos, DS Pismenny, A. Durova, O. Pankova, E. Rodionov, SA Tuzikov, L. Tashireva, V. Perelmuter","doi":"10.24075/brsmu.2022.030","DOIUrl":"https://doi.org/10.24075/brsmu.2022.030","url":null,"abstract":"Lung cancer occupies the leading position in the global structure of oncological diseases. Despite significant advances in its treatment, the survival remains low. Morphological changes to the bronchial epithelium outside the tumor may provide important cues on progression of the disease in patients with lung cancer. This study aimed to identify associations between morphological and clinical features of non-small cell lung cancer and morphological changes to the epithelium in small bronchi outside the tumor. The study encompassed tumor specimens collected from 90 patients, 75 (83%) men and 15 (17%) women, diagnosed with non-small cell lung cancer. The average age of the patients was 67.8 ± 7.4 years. The results indicate higher frequency of lymphogenous metastasis in patients with combined basal cell hyperplasia and squamous metaplasia (BCH+SM+ group) compared to patients with isolated basal cell hyperplasia (BCH+SM– group, p = 0.05). The BCH+SM– group presented with higher rates of hematogenous metastasis compared to BCH+SM+ and BCH–SM– groups (p = 0.004 and p = 0.0019, respectively), as well as increased representation of low-differentiated structures in the primary tumors. The results suggest a commonality of parenchymal-stromal interactions in non-small cell lung cancers and their surroundings and a significant impact of these interactions on differentiation status and progression of the tumors.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43983665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of the human brain potentials variability effects in P300 based brain–computer interface 基于P300脑机接口的人脑电位变异性效应研究

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Bulletin of Russian State Medical University Pub Date : 2022-06-01 DOI: 10.24075/brsmu.2022.033

IP Ganin, A. Kaplan

{"title":"Study of the human brain potentials variability effects in P300 based brain–computer interface","authors":"IP Ganin, A. Kaplan","doi":"10.24075/brsmu.2022.033","DOIUrl":"https://doi.org/10.24075/brsmu.2022.033","url":null,"abstract":"The P300-based brain–computer interfaces (P300 BCI) allow the user to select commands by focusing on them. The technology involves electroencephalographic (EEG) representation of the event-related potentials (ERP) that arise in response to repetitive external stimulation. Conventional procedures for ERP extraction and analysis imply that identical stimuli produce identical responses. However, the floating onset of EEG reactions is a known neurophysiological phenomenon. A failure to account for this source of variability may considerably skew the output and undermine the overall accuracy of the interface. This study aimed to analyze the effects of ERP variability in EEG reactions in order to minimize their influence on P300 BCI command classification accuracy. Healthy subjects aged 21–22 years (n = 12) were presented with a modified P300 BCI matrix moving with specified parameters within the working area. The results strongly support the inherent significance of ERP variability in P300 BCI environments. The correction of peak latencies in single EEG reactions provided a 1.5–2 fold increase in ERP amplitude with a concomitant enhancement of classification accuracy (from 71–78% to 92–95%, p < 0.0005). These effects were particularly pronounced in attention-demanding tasks with the highest matrix velocities. The findings underscore the importance of accounting for ERP variability in advanced BCI systems.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47308456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

InlB protein secreted by Listeria monocytogenes controls the pathogen interaction with macrophages 单核增生李斯特菌分泌的InlB蛋白控制病原体与巨噬细胞的相互作用

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Bulletin of Russian State Medical University Pub Date : 2022-06-01 DOI: 10.24075/brsmu.2022.034

YM Chalenko, MM Abdulkadieva, PV Safarova, E. Kalinin, D. Slonova, SA Yermolaeva

{"title":"InlB protein secreted by Listeria monocytogenes controls the pathogen interaction with macrophages","authors":"YM Chalenko, MM Abdulkadieva, PV Safarova, E. Kalinin, D. Slonova, SA Yermolaeva","doi":"10.24075/brsmu.2022.034","DOIUrl":"https://doi.org/10.24075/brsmu.2022.034","url":null,"abstract":"The virulence of gram-positive bacterium Listeria monocytogenes depends on its capacity to infect non-professional phagocytes and proliferate inside them. Listerias monocytogenes captured by mononuclear phagocytic cells during the infectious process are resistant to lysosomal digestion and can proliferate inside macrophages. Internalin B (InlB), one of the key pathogenicity factors of L. monocytogenes, interacts with mammalian receptors c-Met and gC1q-R. For epithelial cells, such interactions with surface receptors promote activation of these receptors and cytoskeletal remodeling, which leads to massive bacterial invasion into nonprofessional phagocytes. For macrophages, by contrast, nothing is known about the role of InlB in their interactions with L. monocytogenes apart from the fact that both receptors are abundantly expressed by macrophages and participate in the development of immune reactions. This study aimed at determination of the potential role of InlB in the interactions between L. monocytogenes and macrophages. We found that 1) InlB expression promoted a significant 3.5-fold increase in the rates of L. monocytogenes capture by macrophages; 2) the 24 h fold increase in bacterial number inside macrophages constituted 182.5 ± 16.7, 96 ± 12 and 13.3 ± 3 for EGDe∆inlB, EGDe and EGDe∆inlB::pInlB strains, respectively; 3) the EGDe∆inlB::pInlB strain, complemented with a plasmid copy of inlB, produced InlB at 3.3fold higher rates than the type strain EGDe. We conclude that InlB negatively affects the survival of listeria inside macrophages. The results enable advanced understanding of the host-pathogen interactions for L. monocytogenes.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47144899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Predicting preterm birth based on vaginal microbiota assessment by real-time PCR in the first trimester 早期妊娠期阴道微生物群实时PCR评估预测早产

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Bulletin of Russian State Medical University Pub Date : 2022-06-01 DOI: 10.24075/brsmu.2022.032

E. Voroshilina, L. Khayutin, E. Kudryavtseva, VV Kovalev, EE Plotko

引用次数: 0

Alleviation of neurological and cognitive impairments in rat model of ischemic stroke by 0.5 MAC xenon exposure 0.5 MAC氙气暴露对缺血性脑卒中模型大鼠神经和认知功能损害的缓解作用

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Bulletin of Russian State Medical University Pub Date : 2022-06-01 DOI: 10.24075/brsmu.2022.035

IA Krukov, A. Ershov, RA Cherpakov, OA Grebenchikov

{"title":"Alleviation of neurological and cognitive impairments in rat model of ischemic stroke by 0.5 MAC xenon exposure","authors":"IA Krukov, A. Ershov, RA Cherpakov, OA Grebenchikov","doi":"10.24075/brsmu.2022.035","DOIUrl":"https://doi.org/10.24075/brsmu.2022.035","url":null,"abstract":"The majority of stroke patients have cognitive symptoms and about 50% of them live with neurological deficits that critically limit social adaptation capacities even in the absence of significant motor impairments. The aim of this study was to select the optimal length of 0.5 MAC xenon exposure in order to alleviate the neurological and cognitive impairments in experimental stroke. The focal ischemia-reperfusion injury was modeled in rats (n = 70) ising Longa method. The intervention was immediately followed by inhalation of 0.5 MAC xenon for 30, 60 or 120 min. The neurological deficit was assessed using a 'Limb placement' seven-test battery and the cognitive functionalities were assessed by the Morris water maze test. A 30 min 0.5 MAC xenon exposure provided a 40% increase in the limb placement scores and a 17.6% decrease in the Morris water maze test latency compared with the control group (р = 0.055 and р = 0.08, respectively). With a longer 60 min exposure, the trends became significant, the scores improving 2-fold and by 44.4% compared with the control group (р = 0.01 and р = 0.04, respectively), whereas 120 min exposures afforded 2-fold improvements in both tests (р = 0.01). We conclude that, although 30 min post-stroke inhalations provide negligible benefits in terms of neurological status and learning capacity, prolonged exposure times of 60–120 min afford significant improvement in neurological and cognitive indicators and largely alleviate the deteriorating ischemic damage.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49018768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0