单核增生李斯特菌分泌的InlB蛋白控制病原体与巨噬细胞的相互作用

IF 0.2 Q4 MEDICINE, GENERAL & INTERNAL
YM Chalenko, MM Abdulkadieva, PV Safarova, E. Kalinin, D. Slonova, SA Yermolaeva
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引用次数: 1

摘要

革兰氏阳性细菌单核增生李斯特菌的毒力取决于其感染非专业吞噬细胞并在其内部增殖的能力。单核吞噬细胞在感染过程中捕获的单核增生李斯特菌抵抗溶酶体消化,并能在巨噬细胞内增殖。InlB与哺乳动物受体c-Met和gC1q-R相互作用,是单核增生乳杆菌的关键致病因子之一。对于上皮细胞,这种与表面受体的相互作用促进了这些受体的激活和细胞骨架重塑,从而导致大量细菌侵入非专业吞噬细胞。相比之下,对于巨噬细胞来说,除了这两种受体在巨噬细胞中大量表达并参与免疫反应的发展之外,对InlB在它们与单核增生乳杆菌相互作用中的作用一无所知。本研究旨在确定InlB在单核增生乳杆菌与巨噬细胞相互作用中的潜在作用。结果发现:1)表达InlB可使巨噬细胞捕获单核增生乳杆菌的率显著提高3.5倍;2) EGDe∆inlB、EGDe和EGDe∆inlB::pInlB菌株在24 h时巨噬细胞内细菌数量分别增加182.5±16.7、96±12和13.3±3倍;3) EGDe∆inlB::pInlB菌株与inlB的质粒拷贝互补后,inlB的产率比型菌株EGDe高3.3倍。我们得出结论,InlB对巨噬细胞内李斯特菌的存活有负面影响。这些结果使我们对单核增生乳杆菌的宿主-病原体相互作用有了更深入的了解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
InlB protein secreted by Listeria monocytogenes controls the pathogen interaction with macrophages
The virulence of gram-positive bacterium Listeria monocytogenes depends on its capacity to infect non-professional phagocytes and proliferate inside them. Listerias monocytogenes captured by mononuclear phagocytic cells during the infectious process are resistant to lysosomal digestion and can proliferate inside macrophages. Internalin B (InlB), one of the key pathogenicity factors of L. monocytogenes, interacts with mammalian receptors c-Met and gC1q-R. For epithelial cells, such interactions with surface receptors promote activation of these receptors and cytoskeletal remodeling, which leads to massive bacterial invasion into nonprofessional phagocytes. For macrophages, by contrast, nothing is known about the role of InlB in their interactions with L. monocytogenes apart from the fact that both receptors are abundantly expressed by macrophages and participate in the development of immune reactions. This study aimed at determination of the potential role of InlB in the interactions between L. monocytogenes and macrophages. We found that 1) InlB expression promoted a significant 3.5-fold increase in the rates of L. monocytogenes capture by macrophages; 2) the 24 h fold increase in bacterial number inside macrophages constituted 182.5 ± 16.7, 96 ± 12 and 13.3 ± 3 for EGDe∆inlB, EGDe and EGDe∆inlB::pInlB strains, respectively; 3) the EGDe∆inlB::pInlB strain, complemented with a plasmid copy of inlB, produced InlB at 3.3fold higher rates than the type strain EGDe. We conclude that InlB negatively affects the survival of listeria inside macrophages. The results enable advanced understanding of the host-pathogen interactions for L. monocytogenes.
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来源期刊
Bulletin of Russian State Medical University
Bulletin of Russian State Medical University MEDICINE, GENERAL & INTERNAL-
CiteScore
0.80
自引率
0.00%
发文量
59
期刊介绍: Bulletin of Russian State Medical University (Bulletin of RSMU, ISSN Print 2500–1094, ISSN Online 2542–1204) is a peer-reviewed medical journal of Pirogov Russian National Research Medical University (Moscow, Russia). The original language of the journal is Russian (Vestnik Rossiyskogo Gosudarstvennogo Meditsinskogo Universiteta, Vestnik RGMU, ISSN Print 2070–7320, ISSN Online 2070–7339). Founded in 1994, it is issued once every two months publishing articles on clinical medicine and medical and biological sciences, first of all oncology, neurobiology, allergy and immunology, medical genetics, medical microbiology and infectious diseases. Every issue is thematic. Deadlines for manuscript submission are announced in advance. The number of publications on topics in spite of the issue topic is limited. The journal accepts only original articles submitted by their authors, including articles that present methods and techniques, clinical cases and opinions. Authors must guarantee that their work has not been previously published elsewhere in whole or in part and in other languages and is not under consideration by another scientific journal. The journal publishes only one review per issue; the review is ordered by the editors.
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