Predicting preterm birth based on vaginal microbiota assessment by real-time PCR in the first trimester

IF 0.2 Q4 MEDICINE, GENERAL & INTERNAL

Bulletin of Russian State Medical University Pub Date : 2022-06-01 DOI:10.24075/brsmu.2022.032

E. Voroshilina, L. Khayutin, E. Kudryavtseva, VV Kovalev, EE Plotko

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Abstract

Detecting high risk of preterm birth (PB) early makes its prevention possible. The aim of the work was to develop a mathematical predictive model for assessing the risk of preterm birth based on a quantitative analysis of the vaginal microbiota in the first trimester of pregnancy. The study included 199 pregnant women, i.e. 41 pregnancies that ended in preterm birth, and 158 — in term birth. Vaginal microbiota was analyzed in all patients in the 1st trimester of pregnancy by quantitative real-time PCR (qPCR). The method of dicriminant analysis was used to develop a predictive model. A method for predicting PB was developed with the calculation of the PRIMA prognostic index (Premature Birth. Index Of Microbiological Analysis). If the value of PRIMA > 0 – the risk of premature birth is low, if PRIMA < 0 – the risk is high. The sensitivity and specificity of the method are respectively 70.7% and 79.75%, the effectiveness is 77.89%. Evaluation of vaginal microbiota in the 1st trimester makes it possible to identify a high-risk group of PB and perform timely preventive measures.

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早期妊娠期阴道微生物群实时PCR评估预测早产

早期发现高危早产(PB)使其预防成为可能。这项工作的目的是建立一个数学预测模型，基于对怀孕前三个月阴道微生物群的定量分析，来评估早产的风险。该研究包括199名孕妇，即41名孕妇以早产结束，158名以足月结束。采用实时荧光定量PCR (quantitative real-time PCR, qPCR)分析所有妊娠前三个月患者阴道微生物群。采用判别分析方法建立预测模型。通过计算早产儿预后指数(PRIMA)，建立了一种预测早产的方法。微生物分析指标)。如果PRIMA值为0 - 0，早产风险低，如果PRIMA < 0，早产风险高。方法的灵敏度和特异度分别为70.7%和79.75%，有效性为77.89%。在妊娠早期评估阴道微生物群，可以识别高危人群，及时采取预防措施。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bulletin of Russian State Medical University MEDICINE, GENERAL & INTERNAL-

CiteScore

0.80

自引率

0.00%

发文量

期刊介绍： Bulletin of Russian State Medical University (Bulletin of RSMU, ISSN Print 2500–1094, ISSN Online 2542–1204) is a peer-reviewed medical journal of Pirogov Russian National Research Medical University (Moscow, Russia). The original language of the journal is Russian (Vestnik Rossiyskogo Gosudarstvennogo Meditsinskogo Universiteta, Vestnik RGMU, ISSN Print 2070–7320, ISSN Online 2070–7339). Founded in 1994, it is issued once every two months publishing articles on clinical medicine and medical and biological sciences, first of all oncology, neurobiology, allergy and immunology, medical genetics, medical microbiology and infectious diseases. Every issue is thematic. Deadlines for manuscript submission are announced in advance. The number of publications on topics in spite of the issue topic is limited. The journal accepts only original articles submitted by their authors, including articles that present methods and techniques, clinical cases and opinions. Authors must guarantee that their work has not been previously published elsewhere in whole or in part and in other languages and is not under consideration by another scientific journal. The journal publishes only one review per issue; the review is ordered by the editors.