Bulletin of Russian State Medical University最新文献_第10页

A new strategy in selection of hormone therapy for endometrial proliferative process in postmenopausal patients 绝经后子宫内膜增生过程中激素治疗选择的新策略

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Bulletin of Russian State Medical University Pub Date : 2022-06-01 DOI: 10.24075/brsmu.2022.036

GM Savelieva, V.G. Breusenko, E. Kareva, G. Golukhov, D. Gutorova, AV Ovchinnikova, T. Ivanovskaya, KV Shcherbatyuk

{"title":"A new strategy in selection of hormone therapy for endometrial proliferative process in postmenopausal patients","authors":"GM Savelieva, V.G. Breusenko, E. Kareva, G. Golukhov, D. Gutorova, AV Ovchinnikova, T. Ivanovskaya, KV Shcherbatyuk","doi":"10.24075/brsmu.2022.036","DOIUrl":"https://doi.org/10.24075/brsmu.2022.036","url":null,"abstract":"The limited efficacy of hormone therapy for endometrial proliferative process (EPP) in postmenopausal patients and its side effects on the immune system functionalities have not been studied in detail. Here we assess the feasibility of hormone therapy for EPP in postmenopausal patients through evaluation of estradiol and progesterone receptor gene expression in endometrial tissue and peripheral blood mononuclear cells (PBMC). The study enrolled 92 postmenopausal patients with EPP, including 37 pts with glandular-fibrous polyps, 7 pts with non-atypical endometrial hyperplasia (EH), 8 pts with atypical endometrial hyperplasia (AEH), 31 pts with moderately differentiated adenocarcinoma and 9 pts with highly differentiated adenocarcinoma. The PBMC isolates and endometrial samples were tested for ER⍺, ERβ, mER, PRA, PRB, mPR and PGRmC1 expression by reverse real time polymerase chain reaction (RT–PCR). Differential changes in PBMC receptor profiles upon in vitro exposure to progesterone or mifepristone were determined for patients with endometrial polyps and healthy women. The results indicate elevated expression of ERα, ERβ, PRA, PRB, mPR and PGRmC1 by endometrial tissues in EH and elevated expression of mER, ER⍺ and PRA by PBMC in AEH, apparently reflecting suppressed functionalities of monocytes, macrophages, Т-cells and natural killer cells. Unaltered expression of the studied genes by PBMC in endometrial adenocarcinoma may reflect the incrementing tumor autonomy. In vitro, mifepristone inhibited ER⍺, ERβ, mPR, PGRmC1, PRA and PRB expression in PBMC isolated from patients with endometrial polyps. We suppose that such effects can mitigate the negative influence of sex steroid hormones on immunocompetent cells.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49051660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intermembrane oligomerization of SARS-CoV-2 M-protein: possible role in viral budding 严重急性呼吸系统综合征冠状病毒2型M-蛋白的膜间寡聚：可能在病毒出芽中的作用

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Bulletin of Russian State Medical University Pub Date : 2022-06-01 DOI: 10.24075/brsmu.2022.029

E. Sokolinskaya, Lidia V Putlyaeva, AA Gorshkova, KA Lukyanov

引用次数: 0

Isoforms of miR-148a and miR-203a are putative suppressors of colorectal cancer miR-148a和miR-203a的异构体是公认的癌症抑制剂

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Bulletin of Russian State Medical University Pub Date : 2022-05-01 DOI: 10.24075/brsmu.2022.028

S. Nersisyan

{"title":"Isoforms of miR-148a and miR-203a are putative suppressors of colorectal cancer","authors":"S. Nersisyan","doi":"10.24075/brsmu.2022.028","DOIUrl":"https://doi.org/10.24075/brsmu.2022.028","url":null,"abstract":"MicroRNAs are short non-coding molecules which regulate translation in a gene-specific manner. MicroRNA isoforms that differ by few extra or missing nucleotides at the 5'-terminus (5'-isomiR) show strikingly different target specificity. This study aimed to identify functional roles of 5′-isomiR in colorectal cancers. Transcriptomic targets of microRNA isoforms were predicted using bioinformatics tools miRDB and TargetScan. The sets of putative targets identified for 5′-isomiR were integrated with mRNA and microRNA sequencing data for primary colorectal tumors retrieved from The Cancer Genome Atlas Colon Adenocarcinoma (TCGA-COAD) database. The network of interactions among miRNA, their targets and transcription factors was built using the miRGTF-net algorithm. The results indicate that microRNA isoforms highly expressed in colorectal cancer and differing by a single nucleotide position at the 5'-terminus have ≤ 30% common targets. The regulatory network of interactions enables identification of the most engaged microRNA isoforms. Anti-correlated expression levels of canonical microRNA hsa-miR-148a-3p and its putative targets including CSF1, ETS1, FLT1, ITGA5, MEIS1, MITF and RUNX2 proliferation regulators suggest an anti-tumor role for this molecule. The canonical microRNA hsa-miR-203a-3p|0 and its 5′-isoform bind different sets of anti-correlated putative targets, although both of them interact with genes involved in the epithelial-mesenchymal transition: SNAI2 and TNC.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44997657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of ocular manifestation frequency and quality of life in chronic myeloproliferative disorders 慢性骨髓增生性疾病眼部表现频率和生活质量的评估

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Bulletin of Russian State Medical University Pub Date : 2022-05-01 DOI: 10.24075/brsmu.2022.027

E. Yunusova, T. Mukhamadeev, B. Bakirov

{"title":"Assessment of ocular manifestation frequency and quality of life in chronic myeloproliferative disorders","authors":"E. Yunusova, T. Mukhamadeev, B. Bakirov","doi":"10.24075/brsmu.2022.027","DOIUrl":"https://doi.org/10.24075/brsmu.2022.027","url":null,"abstract":"Chronic myeloproliferative disorders (CMPD) include hemoblastoses with abnormal proliferation of myeloid lineages and concomitant alterations in the peripheral blood indicators. The aim of this study was to assess the frequency and structure of ophthalmic complications as a quality of life factor in patients with CMPD. A group of patients with hemoblastoses of this type (n = 41) were surveyed using National Eye Institute Visual Function Questionnaire-25 along with a comprehensive examination by noninvasive ophthalmological techniques. The patients typically reported impaired visual acuity, visual discomfort and foreign body sensation in the eyes. Though many of the patients assessed their general health and vision as satisfactory, the vast majority (68.3%) expressed serious concerns about their visual abilities. The ophthalmological examination revealed various defects including refractive errors (61%), corkscrew dilation and tortuosity of conjunctival and retinal vessels (77.9%), recurrent subconjunctival hemorrhages (39%) and dilated optic nerve sheaths (36.6%). The survey data indicate that visual impairments significantly affect quality of life in patients with CMPD. Overall, the results underscore the importance of interdisciplinary approach in the management of patients with CMPD to enable early diagnosis and feasible correction of the ophthalmic component.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41665760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Altered amino acid profiles of the “mother–fetus” system in COVID-19 新冠肺炎中“母婴”系统氨基酸谱的改变

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Bulletin of Russian State Medical University Pub Date : 2022-05-01 DOI: 10.24075/brsmu.2022.025

N. Lomova, V. Chagovets, E. Dolgopolova, A. Novoselova, Uliana Petrova, R. Shmakov, V. Frankevich

{"title":"Altered amino acid profiles of the “mother–fetus” system in COVID-19","authors":"N. Lomova, V. Chagovets, E. Dolgopolova, A. Novoselova, Uliana Petrova, R. Shmakov, V. Frankevich","doi":"10.24075/brsmu.2022.025","DOIUrl":"https://doi.org/10.24075/brsmu.2022.025","url":null,"abstract":"Systemic nature of the human body response to SARS-CoV-2 requires dedicated analysis at the molecular level. COVID-19 during pregnancy affects maternal health and may entail complications in the early neonatal period and possibly long-term consequences for the offspring. The aim of the study was to assess the impact of COVID-19 on amino acid profiles in maternal venous blood, amniotic fluid and umbilical cord blood in order to develop a diagnostic panel accounting for possible consequences. The main group included 29 pregnant patients with a confirmed diagnosis of COVID-19 and the control group included 17 somatically healthy pregnant women. Amino acid profiles of the biological fluids were measured by high-performance liquid chromatography combined to mass spectrometry (HPLC-MS) and assessed in logistic regression models. The analysis revealed altered content of certain amino acids, their biosynthetic precursors and metabolites in the biological fluids collected from patients with COVID-19 possibly reflecting the development of systemic inflammatory reaction and associated changes in gene expression profiles. These findings may guide further research into health outcomes for neonates born from mothers infected with SARS-CoV-2 during pregnancy. The study may help to develop advanced recommendations and differential care protocols for pregnant women and newborns diagnosed with COVID-19.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46460909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The role of genetic factors in familial case of acne 遗传因素在家族性痤疮中的作用

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Bulletin of Russian State Medical University Pub Date : 2022-05-01 DOI: 10.24075/brsmu.2022.026

O. Demina, A. Rumyantsev, NN Potekaev

引用次数: 0

Transcriptional profiling of Mycobacterium smegmatis exposed to subinhibitory concentrations of G4-stabilizing ligands 暴露于低抑制浓度G4稳定配体的耻垢分枝杆菌的转录谱

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Bulletin of Russian State Medical University Pub Date : 2022-05-01 DOI: 10.24075/brsmu.2022.024

MV Zaychikova, D. Bespiatykh, M. Malakhova, IN Bodoev, TS Vedekhina, V. Veselovsky, KM Klimina, AM Varizhuk, E. Shitikov

{"title":"Transcriptional profiling of Mycobacterium smegmatis exposed to subinhibitory concentrations of G4-stabilizing ligands","authors":"MV Zaychikova, D. Bespiatykh, M. Malakhova, IN Bodoev, TS Vedekhina, V. Veselovsky, KM Klimina, AM Varizhuk, E. Shitikov","doi":"10.24075/brsmu.2022.024","DOIUrl":"https://doi.org/10.24075/brsmu.2022.024","url":null,"abstract":"The spread of Mycobacterium tuberculosis drug resistance accentuates the demand for anti-tuberculosis drugs with a fundamentally new mechanism of action without conferring cross-resistance. G-quadruplexes (G4, non-canonical DNA structures) are plausible new drug targets. Although G4-stabilizing ligands have been shown to inhibit mycobacterial growth, the exact mechanism of their action is uncertain. The aim of this study was to assess a possible correlation between putative G4 elements in a model mycobacterial strain M. smegmatis MC2155 and transcriptomic changes under the action of subinhibitory concentrations of G4 ligands BRACO-19 and TMPyP4. We also planned to compare the results with corresponding data previously obtained by us using higher, inhibitory concentrations of these ligands. For BRACO-19, we identified 589 (316↑; 273↓) and 865 (555↑; 310↓) differentially expressed genes at 5 µМ and 10 µМ, respectively. For TMPyP4, we observed the opposite trend, the number of differentially expressed genes decreased at higher concentration of the ligand: 754 (337↑; 417↓) and 702 (359↑; 343↓) for 2 µМ and 4 µМ, respectively. Statistical analysis revealed no correlation between ligand-induced transcriptomic changes and genomic localization of the putative quadruplex-forming sequences. At the same time, the data indicate certain functional specificity of the ligand-mediated transcriptomic effects, with TMPyP4 significantly affecting expression levels of transcription factors and arginine biosynthesis genes and BRACO-19 significantly affecting expression levels of iron metabolism and replication and reparation system genes.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48477864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a recombinant oncolytic poliovirus type 3 strain with altered cell tropism 细胞嗜性改变的重组溶瘤性脊髓灰质炎病毒3型毒株的研制

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Bulletin of Russian State Medical University Pub Date : 2022-04-01 DOI: 10.24075/brsmu.2022.023

Вестник Ргму, VESTNIKRGMU.RU Hamad, A. Soboleva, P. Vorobyev, K. Vasilenko, P. Chumakov, A. Lipatova, A. Lipatova

引用次数: 1

Regenerative effects of Gly-His-Lys and Gly-His-Lys-D-Ala peptides in infected skin wounds Gly-His-Lys-D-Ala肽对感染性皮肤创伤的再生作用

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Bulletin of Russian State Medical University Pub Date : 2022-04-01 DOI: 10.24075/brsmu.2022.014

K. Rakhmetova, E. Mishina, A. Vorvul, I. Bobyntsev, Maksim E. Dolgintsev, A. Bezhin

{"title":"Regenerative effects of Gly-His-Lys and Gly-His-Lys-D-Ala peptides in infected skin wounds","authors":"K. Rakhmetova, E. Mishina, A. Vorvul, I. Bobyntsev, Maksim E. Dolgintsev, A. Bezhin","doi":"10.24075/brsmu.2022.014","DOIUrl":"https://doi.org/10.24075/brsmu.2022.014","url":null,"abstract":"Skin wound healing mechanisms and new ways of improving their efficiency represent an important focus in medicine. In this regard, regulatory peptides, which exhibit physiological polyfunctionality and modulate cell growth and differentiation, are of special interest. This study evaluates the effects of Gly-His-Lys (GHK) and Gly-His-Lys-D-Ala (GHK-D-Ala) peptides in the infected skin wound healing. The wounds were modeled in rats (n=150) by full-thickness dorsal skin defects. The peptides were administered intracutaneously at daily doses of 0.5 or 1.5 µg/kg. The healing was assessed on days 3, 7, and 10 by histomorphometric examination of the wounds with adjacent intact skin. GHK-D-Ala administered at daily doses of 0.5 µg/kg had pronounced positive effect on regeneration processes in the wound, as indicated by significantly reduced numbers of granulocytes and lymphocytes with increased representation of fibroblastic lineages and macrophages, and the resulting higher cellular index (p < 0.05–0.001). At higher doses of GHK-D-Ala (1.5 µg/kg), the beneficial effects were less pronounced. According to the comparative morphological examination, the highest positive effect was achieved with 0.5 µg/kg of GHK-D-Ala. Thus, local administration of the GHK peptide with extra D-alanine at carboxy-terminus significantly mitigated the inflammatory reaction and facilitated the healing of infected skin wounds in rat model.","PeriodicalId":9344,"journal":{"name":"Bulletin of Russian State Medical University","volume":" ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45882946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic non-treated posterior fracture-dislocation of the shoulder 慢性非治疗性肩后骨折脱位

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Bulletin of Russian State Medical University Pub Date : 2022-04-01 DOI: 10.24075/brsmu.2022.022

K. Egiazaryan, DS Ershov, D. Badriev, DY Soshnikov

引用次数: 1