Intermembrane oligomerization of SARS-CoV-2 M-protein: possible role in viral budding

IF 0.2 Q4 MEDICINE, GENERAL & INTERNAL

Bulletin of Russian State Medical University Pub Date : 2022-06-01 DOI:10.24075/brsmu.2022.029

E. Sokolinskaya, Lidia V Putlyaeva, AA Gorshkova, KA Lukyanov

引用次数: 0

Abstract

Despite the extensive research spurred by the catastrophic effects of COVID-19 pandemic, precise molecular mechanisms of some stages in SARS-CoV-2 life cycle remain elusive. One of such stages is the detachment of viral particles during budding. Using confocal fluorescence microscopy, we observed formation of specific structures by endoplasmic reticulum in human cells expressing SARS-CoV-2 M-protein, implicating oligomerization of M-protein across parallel membranes. In our opinion, such intermembrane oligomerization may provide a driving force for pinching off the viral particles during SARS-CoV-2 budding.

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严重急性呼吸系统综合征冠状病毒2型M-蛋白的膜间寡聚：可能在病毒出芽中的作用

尽管新冠肺炎大流行的灾难性影响刺激了广泛的研究，但SARS-CoV-2生命周期某些阶段的确切分子机制仍然难以捉摸。其中一个阶段是出芽过程中病毒颗粒的分离。使用共聚焦荧光显微镜，我们观察到表达严重急性呼吸系统综合征冠状病毒2型M-蛋白的人类细胞中内质网形成特定结构，这表明M-蛋白在平行膜上低聚。在我们看来，这种膜间低聚可能为在严重急性呼吸系统综合征冠状病毒2型出芽过程中掐断病毒颗粒提供驱动力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bulletin of Russian State Medical University MEDICINE, GENERAL & INTERNAL-

CiteScore

0.80

自引率

0.00%

发文量

期刊介绍： Bulletin of Russian State Medical University (Bulletin of RSMU, ISSN Print 2500–1094, ISSN Online 2542–1204) is a peer-reviewed medical journal of Pirogov Russian National Research Medical University (Moscow, Russia). The original language of the journal is Russian (Vestnik Rossiyskogo Gosudarstvennogo Meditsinskogo Universiteta, Vestnik RGMU, ISSN Print 2070–7320, ISSN Online 2070–7339). Founded in 1994, it is issued once every two months publishing articles on clinical medicine and medical and biological sciences, first of all oncology, neurobiology, allergy and immunology, medical genetics, medical microbiology and infectious diseases. Every issue is thematic. Deadlines for manuscript submission are announced in advance. The number of publications on topics in spite of the issue topic is limited. The journal accepts only original articles submitted by their authors, including articles that present methods and techniques, clinical cases and opinions. Authors must guarantee that their work has not been previously published elsewhere in whole or in part and in other languages and is not under consideration by another scientific journal. The journal publishes only one review per issue; the review is ordered by the editors.