BMC Research Notes最新文献_第5页

Inefficacy of a novel osmotic associative learning assay in C. elegans. 一种新的渗透联想学习试验在秀丽隐杆线虫中的无效。

IF 1.7

BMC Research Notes Pub Date : 2026-06-30 DOI: 10.1186/s13104-026-07928-x

Jin Hua Li, Kaiden H Price, Michael N Nitabach

{"title":"Inefficacy of a novel osmotic associative learning assay in C. elegans.","authors":"Jin Hua Li, Kaiden H Price, Michael N Nitabach","doi":"10.1186/s13104-026-07928-x","DOIUrl":"10.1186/s13104-026-07928-x","url":null,"abstract":"Objective: In this study, we developed and tested a novel osmotic associative assay for facilitating the study of associative learning circuits with constrained sensory inputs in Caenorhabditis elegans (C. elegans).Results: Hyperosmolarity, the unconditioned stimulus, was paired with benzaldehyde, an attractive odorant here used as a conditioned stimulus, in order to train an association between hyperosmolarity and odor. It was hypothesized that animals conditioned in hyperosmotic conditions for 90 min would show reduced chemotaxis to benzaldehyde compared to control animals. Results suggest that, following exposure to a hyperosmolar 500mM fructose solution, C. elegans chemotaxis to benzaldehyde was impaired. At more moderate hyperosmolarity, worms failed to learn to associate hyperosmolarity with benzaldehyde - i.e., there was no difference between chemotaxis index values of conditioned and naive worms in any of the fructose concentrations. Thus, our results both suggest that 90-minute associative learning assays in C. elegans may not succeed with hyperosmolarity as an unconditioned stimulus, and provide a cautionary note that researchers using hyperosmotic solutions in worm behavior assays should be cognizant of potential effects on naïve chemotaxis behavior.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13587300/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148359532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypical and functional characterization of a HepG2 cell clone stably overexpressing cytochrome P450 (CYP) 2C9. 稳定过表达细胞色素P450 (CYP) 2C9的HepG2细胞克隆的表型和功能特征

IF 1.7

BMC Research Notes Pub Date : 2026-06-30 DOI: 10.1186/s13104-026-07935-y

Sarah Kammerer, Natalie Herzog, Thea Jenchen, Valentina Stock, Rebecca Hofer, Veronika Ruzsanyi, Jan-Heiner Küpper

{"title":"Phenotypical and functional characterization of a HepG2 cell clone stably overexpressing cytochrome P450 (CYP) 2C9.","authors":"Sarah Kammerer, Natalie Herzog, Thea Jenchen, Valentina Stock, Rebecca Hofer, Veronika Ruzsanyi, Jan-Heiner Küpper","doi":"10.1186/s13104-026-07935-y","DOIUrl":"10.1186/s13104-026-07935-y","url":null,"abstract":"Objective: We aimed to generate a HepG2 cell clone stably overexpressing cytochrome P450 (CYP) 2C9 together with an empty vector (EV) control cell clone for pharmacological and toxicological studies of substances with CYP2C9-mediated biotransformation.Results: A new HepG2 cell clone was generated by lentiviral transduction to functionally overexpress human CYP2C9. We found high CYP2C9 transcript and protein levels based on qRT-PCR, Western blot and immunofluorescence in the cell clone. Most importantly, specific enzyme activities of 62.9 ± 2.6 pmol 4-hydroxydiclofenac/min/106 cells as analyzed by diclofenac conversion via liquid chromatography-mass spectrometry were found in HepG2-CYP2C9 cells. CYP2C9 enzyme activity could successfully be inhibited by the application of the pan-CYP inhibitor 1-aminobenzotriazole or the CYP2C9-specific inhibitor sulfaphenazole. No changes in morphology or population doubling times were detected when comparing the clone to parental HepG2 cells. The corresponding EV control cell line was detected negative for CYP2C9 expression in all experimental setups and did not show any difference to the parental HepG2 cells. Thus, the newly established HepG2-CYP2C9 cell line is an appropriate tool to study metabolism and toxicity of substances depending on conversion by CYP2C9.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"19 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13321531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148359607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anticancer proteasome inhibitors are detrimental to the growth of Toxoplasma gondii in vitro. 抗癌蛋白酶体抑制剂对刚地弓形虫体外生长不利。

IF 1.7

BMC Research Notes Pub Date : 2026-06-29 DOI: 10.1186/s13104-026-07930-3

Meir Zhang, Pascale S Guiton

引用次数: 0

Comparative hemostatic efficacy of oxidized regenerated cellulose powder and fibrin glue in a rat model of induced liver parenchymal hemorrhage. 氧化再生纤维素粉与纤维蛋白胶在大鼠肝实质出血模型中的止血效果比较。

IF 1.7

BMC Research Notes Pub Date : 2026-06-27 DOI: 10.1186/s13104-026-07929-w

Berkan Acar, Abdulkadir Çelik, Arslan Hasan Kocamaz, Ömer Kişi, Alper Varman, Selman Alkan, Sıdıka Fındık, İbrahim Kılınç, Mustafa Şentürk, Tevfik Küçükkartallar

{"title":"Comparative hemostatic efficacy of oxidized regenerated cellulose powder and fibrin glue in a rat model of induced liver parenchymal hemorrhage.","authors":"Berkan Acar, Abdulkadir Çelik, Arslan Hasan Kocamaz, Ömer Kişi, Alper Varman, Selman Alkan, Sıdıka Fındık, İbrahim Kılınç, Mustafa Şentürk, Tevfik Küçükkartallar","doi":"10.1186/s13104-026-07929-w","DOIUrl":"10.1186/s13104-026-07929-w","url":null,"abstract":"Aim: The objective of this study is to compare the hemostatic efficacy of oxidised regenerated cellulose powder (Surgicel® Powder) and fibrin glue (Tisseel®) in a rat model of liver parenchymal haemorrhage, and to evaluate their effects on tissue healing.Results: Preoperative haemoglobin and haematocrit values were comparable among the groups (p > 0.05). At 24 h, postoperative haemoglobin and haematocrit values differed significantly among the three groups (p = 0.045 and p = 0.042, respectively), with lower values in the saline-compression control group and relatively preserved values in the Surgicel® Powder and Tisseel® groups. However, no statistically significant difference was identified between the two active treatment groups. Histopathological evaluation revealed no statistically significant between-group differences in necrosis, inflammation, fibrosis, haemorrhage, or granulation tissue formation (p > 0.05).","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13621728/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148337830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using LLM-generated tools to extract information about reporting statistical software in biomedical and health science research articles. 使用法学硕士生成的工具提取生物医学和健康科学研究文章中报告统计软件的信息。

IF 1.7

BMC Research Notes Pub Date : 2026-06-27 DOI: 10.1186/s13104-026-07908-1

Diego A Forero, Pentti Nieminen

{"title":"Using LLM-generated tools to extract information about reporting statistical software in biomedical and health science research articles.","authors":"Diego A Forero, Pentti Nieminen","doi":"10.1186/s13104-026-07908-1","DOIUrl":"10.1186/s13104-026-07908-1","url":null,"abstract":"Objective: A major problem with reviewing the statistical methodology in published medical articles is that extracting the necessary details from large sample sets is time-consuming. This paper demonstrates how a novel automated procedure can extract information about statistical reporting from literature. To illustrate this, we searched the PubMed Central database for original research articles published in 2021 and 2023 to identify the statistical software packages used for data analysis. A key element in terms of transparency and reproducibility is the reporting of the software used for statistical analysis.Results: A freely available Shiny App was created with the help of generative artificial intelligence, and it was used to retrieve automatically information from randomly selected samples of articles indexed in PubMed Central. We analyzed a large sample of articles (n = 1740) to determine the reporting of statistical software for nine study designs. We found that, across different study types, proprietary software such as IBM SPSS Statistics still dominates. Despite multiple calls for greater use of open-source research software, these programs are not used as frequently. In addition, a surprising number of articles did not report the software used. Furthermore, this is the first application of the recent Vibe Coding concept to statistical research methods.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13352756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148337956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Body mass index, nutritional knowledge, and eating attitudes in dancer and non-dancer students. 舞蹈学生和非舞蹈学生的体重指数、营养知识和饮食态度。

IF 1.7

BMC Research Notes Pub Date : 2026-06-27 DOI: 10.1186/s13104-026-07932-1

Caifang Qiu, Hui Wang

{"title":"Body mass index, nutritional knowledge, and eating attitudes in dancer and non-dancer students.","authors":"Caifang Qiu, Hui Wang","doi":"10.1186/s13104-026-07932-1","DOIUrl":"10.1186/s13104-026-07932-1","url":null,"abstract":"Background: This cross-sectional comparative study examined differences in body mass index (BMI), nutritional knowledge, and eating behaviors between dancer and non-dancer university students. Given the physical and aesthetic demands of dance, understanding potential differences in health-related characteristics in this population is of particular importance.Methods: Seventy female undergraduates (35 dancers and 35 non-dancers, aged 17-22 years) were recruited. Height, weight, and BMI were measured using the InBody 970 analyzer. Nutritional knowledge was assessed using the Chinese version of the General Nutrition Knowledge Questionnaire (C-GNKQ), and disordered eating risk was evaluated using the Eating Attitudes Test-26 (EAT-26)©. Group differences were analyzed using independent t-tests, with effect sizes (Cohen's d) calculated to estimate the magnitude of differences.Results: Dancers were significantly taller than non-dancers (P = 0.003, d = 0.74) and had a lower BMI (P = 0.023, d = 0.82). No statistically significant difference was observed in overall C-GNKQ scores (P = 0.128, d = 0.37), although dancers scored higher in the dietary knowledge subscale (P = 0.037, d = 0.52). EAT-26© scores were higher in dancers, but the difference was not statistically significant (P = 0.147, d = 0.35). Additional Spearman correlation analyses revealed no significant associations among BMI, C-GNKQ scores, and EAT-26© scores (P > 0.05).","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13573465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148337884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soluble CD25 as a potential prognostic biomarker for pediatric septic shock and its comparison with hemophagocytic lymphohistiocytosis. 可溶性CD25作为儿童感染性休克的潜在预后生物标志物及其与噬血细胞淋巴组织细胞增生症的比较

IF 1.7

BMC Research Notes Pub Date : 2026-06-26 DOI: 10.1186/s13104-026-07923-2

T B Garcia, V Knight, H F Scott

{"title":"Soluble CD25 as a potential prognostic biomarker for pediatric septic shock and its comparison with hemophagocytic lymphohistiocytosis.","authors":"T B Garcia, V Knight, H F Scott","doi":"10.1186/s13104-026-07923-2","DOIUrl":"10.1186/s13104-026-07923-2","url":null,"abstract":"Objectives: Identifying children at highest risk for deterioration during evaluation for septic shock in emergency department (ED) settings remains challenging. Soluble CD25 (sCD25), a hemophagocytic lymphohistiocytosis (HLH) diagnostic criterion, has been associated with sepsis mortality in ICU settings. This study aimed to determine whether sCD25 is associated with adverse outcomes in children undergoing initial septic shock evaluation in a pediatric ED. We also sought to compare sCD25 in patients with septic shock, infection without sepsis, and HLH.Results: Children with HLH demonstrated significantly higher sCD25 levels [25,800 pg/mL (IQR: 1620-42,300)] compared with those with septic shock [5200 pg/mL (IQR: 2500-20,600)] or infection [5500 pg/mL (IQR: 2200-12,500)]. No statistical differences were observed between septic shock and infection groups; however, a subset of septic shock patients exhibited sCD25 elevations comparable to HLH patients. These patients experienced severe outcomes, including 100% PICU admission, 50% vasoactive support requirement, and prolonged hospitalization (16 days). Bloodstream infections were associated with the highest sCD25 levels. These findings suggest sCD25 elevation may be associated with adverse outcomes in children with septic shock. Larger studies are warranted to validate sCD25 as a prognostic marker for children with septic shock.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13563836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148337967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple scanner-based colorimetric method for fluoride determination using Alfusone reagent. 用Alfusone试剂测定氟化物的简易扫描比色法。

IF 1.7

BMC Research Notes Pub Date : 2026-06-26 DOI: 10.1186/s13104-026-07926-z

Masahide Hagiri, Shion Nomura, Erika Fukasawa, Takeshi Kato, Atsushi Manaka

{"title":"A simple scanner-based colorimetric method for fluoride determination using Alfusone reagent.","authors":"Masahide Hagiri, Shion Nomura, Erika Fukasawa, Takeshi Kato, Atsushi Manaka","doi":"10.1186/s13104-026-07926-z","DOIUrl":"10.1186/s13104-026-07926-z","url":null,"abstract":"Objective: The quantitative determination of fluoride ions is important for water quality monitoring and public health. This study aimed to develop a simple and simultaneous multi-sample digital colorimetric method for fluoride determination using a 96-well plate and a flatbed scanner, without requiring conventional analytical instruments.Results: Fluoride solutions were color-developed using Alfusone reagent in a 96-well plate and scanned with a flatbed scanner. Color information was extracted from the scanned images and analyzed using red-green-blue (RGB), cyan-magenta-yellow-key (CMYK), hue-saturation-value (HSV), CIE XYZ, and CIELAB parameters. Among the evaluated parameters, the HSV H parameter showed the lowest limit of detection and limit of quantification of 0.086 and 0.29 mg/L, respectively. The CIELAB b* parameter showed the lowest values among the evaluated CIELAB parameters, with a limit of detection and limit of quantification of 0.19 and 0.64 mg/L, respectively. These values were below the World Health Organization guideline value for fluoride and the Japanese drinking water quality standard. Spike-and-recovery tests using bottled and tap water showed that the R and a* parameters provided recoveries closer to 100% than b*. The method offers a simple, attachment-free approach that enables simultaneous analysis of multiple samples and calibration standards using commercially available reagents and office automation equipment.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13563788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148337842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Admission BNP-dominant biomarker phenotypes and in-hospital heart failure or cardiogenic shock in STEMI: a retrospective research note. 入院bnp显性生物标志物表型和住院心力衰竭或心源性休克在STEMI：回顾性研究笔记。

IF 1.7

BMC Research Notes Pub Date : 2026-06-25 DOI: 10.1186/s13104-026-07927-y

Quan Zuo, Li Zhao, Tao Ge

{"title":"Admission BNP-dominant biomarker phenotypes and in-hospital heart failure or cardiogenic shock in STEMI: a retrospective research note.","authors":"Quan Zuo, Li Zhao, Tao Ge","doi":"10.1186/s13104-026-07927-y","DOIUrl":"10.1186/s13104-026-07927-y","url":null,"abstract":"Objective: To assess, in a single-center retrospective cohort, whether admission B-type natriuretic peptide (BNP)-dominant biomarker phenotypes incorporating D-dimer are associated with in-hospital heart failure or cardiogenic shock (HF/CS) in patients with ST-segment elevation myocardial infarction (STEMI).Results: Among 509 unique STEMI admissions, 467 had analyzable admission timestamps. The biomarker-enriched cohort included 140 patients with BNP and D-dimer measured within 24 h; 64 (45.7%) developed HF/CS. In the complete-case adjusted cohort (n = 136; 62 events), an equal-weight burden score [z(ln BNP) + z(ln D-dimer)] was associated with HF/CS (adjusted odds ratio [OR] 4.04, 95% confidence interval [CI] 1.84-8.92). BNP alone showed a similarly strong association (adjusted OR 3.75, 95% CI 1.86-7.60) and a slightly higher apparent area under the curve (0.859 vs. 0.849), indicating that the signal was mainly BNP-driven. The high BNP/high D-dimer phenotype had the highest HF/CS rate (80.9%). These findings should be interpreted as exploratory association and risk-enrichment observations rather than validated prediction or a complete assessment of congestion status.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13555971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148326880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Capturing goal content from free text in weight loss: an exploratory pilot on feasibility, reliability, and early outcomes. 在减肥中从自由文本中获取目标内容：可行性、可靠性和早期结果的探索性试验。

IF 1.7

BMC Research Notes Pub Date : 2026-06-25 DOI: 10.1186/s13104-026-07925-0

Tomomi Nagasawa, Tsuyoshi Okuhara, Yuriko Nishiie, Hiroko Okada, Takahiro Kiuchi

{"title":"Capturing goal content from free text in weight loss: an exploratory pilot on feasibility, reliability, and early outcomes.","authors":"Tomomi Nagasawa, Tsuyoshi Okuhara, Yuriko Nishiie, Hiroko Okada, Takahiro Kiuchi","doi":"10.1186/s13104-026-07925-0","DOIUrl":"10.1186/s13104-026-07925-0","url":null,"abstract":"Objective: Understanding patients' self-articulated weight-loss goals may support tailored weight-management interventions; however, reproducible methods to capture and classify free-text goal content are limited. This pilot study aimed to develop and preliminarily evaluate a laddering-informed free-text elicitation and classification protocol, and to explore its associations with motivational quality and short-term weight change.Results: Adults with a body mass index ≥ 25 kg/m² enrolled in a 3-month digital behavioral weight-management program completed two free-text prompts. Responses were coded by two trained raters, using rules mapped to the Aspiration Index (AI) and the Goal Content for Weight Maintenance Scale (GCWMS). Inter-rater agreement was excellent (Cohen's κ = 0.91 for both frameworks). The protocol yielded analyzable data. AI showed better operational fit than GCWMS, with fewer unclassified (\"Other\") responses (2.1% vs. 19.1%). Differences in motivational quality across goal-content categories were small (partial η² = 0.005-0.032). In exploratory models, engagement was positively associated with weight loss, whereas autonomous motivation showed a negative association over the short observation period.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13560303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148326945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0