BMC Research Notes最新文献_第4页

Feasibility of delivering a digital multiple health behaviour intervention via a home-telemonitoring system: engagement, outcomes, and user experiences. 通过家庭远程监测系统提供数字多种健康行为干预的可行性：参与、结果和用户体验。

IF 1.7

BMC Research Notes Pub Date : 2026-07-07 DOI: 10.1186/s13104-026-07915-2

Elizabeth S Collier, Marie Löf, Preben Bendtsen, Joel Crawford, Marcus Bendtsen

{"title":"Feasibility of delivering a digital multiple health behaviour intervention via a home-telemonitoring system: engagement, outcomes, and user experiences.","authors":"Elizabeth S Collier, Marie Löf, Preben Bendtsen, Joel Crawford, Marcus Bendtsen","doi":"10.1186/s13104-026-07915-2","DOIUrl":"10.1186/s13104-026-07915-2","url":null,"abstract":"Objective: Finding novels ways to deliver digital health behaviour interventions could increase their reach and impact. The aim of this study was to evaluate the feasibility of using a home-telemonitoring system (offered to eligible patients by regional authorities in Sweden for symptom- and health marker monitoring) as a novel dissemination opportunity. The intervention has been previously found effective at changing health behaviours in a general online help-seeking population. In the present study, participants were not randomised: all had access to the intervention (pre-post analyses).Results: Among 416 patients offered the digital intervention, 12.0% (n = 50) signed up, 58.0% (n = 29) of whom completed the 16-week follow-up. After 16-weeks of intervention access, participants reported increased fruit and vegetable intake (median = + 95.4 g/day) and decreased candy consumption (median= -58.4 g/week), but little change in alcohol consumption or physical activity. Changes were non-linear throughout the study, with the largest changes observed at the beginning and after 8-week follow-up. Participants were generally satisfied with the support, though concerns of personal relevance, disproportionate personal responsibility, low content specificity, and frustration with repetitive reminders were noted. With intervention tailoring to the population and delivery system, home-telemonitoring systems could offer a way to increase proactive intervention reach beyond individuals already seeking help.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"19 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13339708/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148396022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of the FTO rs9939609 variant with glycemic control based on fasting glucose. FTO rs9939609变异与基于空腹血糖的血糖控制的关联

IF 1.7

BMC Research Notes Pub Date : 2026-07-07 DOI: 10.1186/s13104-026-07933-0

Nicolas Fragoso-Bargas, Rocio Vanessa Escarcega-Castro, Irma Quintal-Ortiz, Ligia Vera-Gamboa, Guillermo Valencia-Pacheco, Nina Valadez-Gonzalez

{"title":"Association of the FTO rs9939609 variant with glycemic control based on fasting glucose.","authors":"Nicolas Fragoso-Bargas, Rocio Vanessa Escarcega-Castro, Irma Quintal-Ortiz, Ligia Vera-Gamboa, Guillermo Valencia-Pacheco, Nina Valadez-Gonzalez","doi":"10.1186/s13104-026-07933-0","DOIUrl":"10.1186/s13104-026-07933-0","url":null,"abstract":"Background: Type 2 diabetes (T2D) affects 11.1% of the global population, underscoring the need for biomarkers that help characterize glycemic control status among treated individuals. We evaluated the association between the FTO variant rs9939609‑A and glycemic control in a Mexican population.Methods: A total of 174 individuals living with T2D from Mérida and Sisal, Yucatán, were included, of whom 85% were receiving oral hypoglycemic agents as main treatment. Glycemic control was defined cross‑sectionally as good (≤ 130 mg/dL, n = 63) or poor (> 130 mg/dL, n = 111) with fasting glucose. Linear mixed models incorporating relevant covariates and a family random intercept were used. Effect size estimates were transformed to logit odds ratios.Results: After adjustment for age, sex, BMI, years since T2D diagnosis, and treatment, the minor allele of rs9939609 (A) was associated with an increased risk of poorer glycemic control, reaching significance under both the additive (OR = 1.145 [1.003-1.307], p = 0.047) and recessive (OR = 1.514 [1.026-2.234], p = 0.038) models. In an alternative model adjusting for waist circumference instead of BMI, the effect of rs9939609‑A in the additive model was slightly attenuated (OR = 1.135 [0.994-1.297], p = 0.063), while the recessive model remained significant (OR = 1.486 [1.010-2.189], p = 0.046).Conclusions: rs9939609-A was associated with poorer glycemic control in this exploratory cohort, but replication in larger and ancestrally characterized samples is required.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13625294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148403491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of functional and social factors with domain-specific quality of life profiles in children with cerebral palsy: findings from a low- and middle-income country. 功能和社会因素与脑瘫儿童特定领域生活质量概况的关联：来自中低收入国家的调查结果

IF 1.7

BMC Research Notes Pub Date : 2026-07-07 DOI: 10.1186/s13104-026-07943-y

S A C Dalpatadu, T C M G D P Cooray, Chryshanth Dalpatadu

{"title":"Association of functional and social factors with domain-specific quality of life profiles in children with cerebral palsy: findings from a low- and middle-income country.","authors":"S A C Dalpatadu, T C M G D P Cooray, Chryshanth Dalpatadu","doi":"10.1186/s13104-026-07943-y","DOIUrl":"10.1186/s13104-026-07943-y","url":null,"abstract":"Objective: To evaluate domain-specific quality of life and its associations with functional severity, selected comorbidities, and social factors among children with cerebral palsy attending a tertiary referral centre in Sri Lanka.Results: Among 223 children, 61.9% were male and the mean age was 6.99 years. Quality-of-life scores varied markedly across domains. Daily activities, school activities, movement and balance, eating activities, and speech and communication showed wide score dispersion, whereas pain and fatigue showed ceiling effects. Scores declined progressively with increasing Gross Motor Function Classification System level across analysed domains, with strong monotonic trends observed (Kendall's τ - 0.57 to - 0.72; all p < 0.001). Epilepsy, cognitive impairment, cortico-visual impairment, hearing impairment, and gastro-oesophageal reflux disease were associated with lower overall or domain-specific scores in unadjusted analyses. In adjusted models focused on measured social factors, age, sex, living arrangement, and parental education were not independently associated with the analysed domain scores. Domain-level assessment may provide clinically informative quality-of-life profiles that are not captured by overall scores alone.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13625381/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148403537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Implementation of daily objectives for the reduction of multidrug-resistant infections in an intensive care unit. 在重症监护病房实施减少耐多药感染的日常目标。

IF 1.7

BMC Research Notes Pub Date : 2026-07-07 DOI: 10.1186/s13104-026-07945-w

Aarón Humberto Ramírez-Mora, Benito Manuel Flores-Pastor, Beatriz Victoria-Moreno, Nicolás Padilla-Raygoza

引用次数: 0

From manual entry to machine precision: challenges and evolution of metadata schema development in collaborative research centers. 从人工输入到机器精度：协作研究中心元数据模式开发的挑战和演变。

IF 1.7

BMC Research Notes Pub Date : 2026-07-06 DOI: 10.1186/s13104-026-07937-w

Felix Engel, Claudia Giuliani, Manuel Watter, Aref Kalantari, Karin Schuller, Harald Binder, Klaus Kaier

{"title":"From manual entry to machine precision: challenges and evolution of metadata schema development in collaborative research centers.","authors":"Felix Engel, Claudia Giuliani, Manuel Watter, Aref Kalantari, Karin Schuller, Harald Binder, Klaus Kaier","doi":"10.1186/s13104-026-07937-w","DOIUrl":"10.1186/s13104-026-07937-w","url":null,"abstract":"Objective: Metadata standardization in collaborative biomedical research must balance interoperability with domain-specific detail. We describe a parent-template approach in which a baseline schema from the nephrology-focused CRC 1453 NephGen was adapted for the tumor-immunology CRC OncoEscape and the perinatal-immunology CRC Pilot.Results: The derivation process produced three structurally compatible yet vocabulary-divergent schemas. Pilot required the highest granularity (324 levels), followed by NephGen (287) and OncoEscape (283). Vocabulary reuse from the NephGen baseline was limited: 134 of 283 OncoEscape levels (47%) and 113 of 324 Pilot levels (35%) were retained unchanged. The main adaptations were not only expanded level lists, such as cell lines and mouse lines, but also new CRC-specific query dimensions, including \"Oncogenes\" in OncoEscape and \"Timeline\" in Pilot. In the context of AI-assisted extraction, we use the term instruction set to denote a schema that specifies target fields, expected granularity, example values, and validation resources for each metadata dimension, rather than a simple drop-down form or a free-text prompt template.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"19 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13339696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148396018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sleep deprivation impairs gastric ulcer healing and induces anxiety-like behavior in rats. 睡眠剥夺会损害大鼠胃溃疡愈合并诱发焦虑样行为。

IF 1.7

BMC Research Notes Pub Date : 2026-07-03 DOI: 10.1186/s13104-026-07936-x

Omosola Fisayo Anifowose, Moshood Abiola Folawiyo, Temitope Elizabeth Olabiyi, Oluwabunmi Grace Benson, Durotioluwa Lois Olu-Agbede, Gbenga Sunday Adeleye, Oluwatomisin Olalekan Faniyan, Kehinde Samuel Olaniyi

{"title":"Sleep deprivation impairs gastric ulcer healing and induces anxiety-like behavior in rats.","authors":"Omosola Fisayo Anifowose, Moshood Abiola Folawiyo, Temitope Elizabeth Olabiyi, Oluwabunmi Grace Benson, Durotioluwa Lois Olu-Agbede, Gbenga Sunday Adeleye, Oluwatomisin Olalekan Faniyan, Kehinde Samuel Olaniyi","doi":"10.1186/s13104-026-07936-x","DOIUrl":"10.1186/s13104-026-07936-x","url":null,"abstract":"Objective: This study investigated the effects of total sleep deprivation (SD) on gastric ulcer (GU) healing, anxiety-like behavior, neuro-oxidative stress, antioxidant defenses, and prefrontal cortical histoarchitecture in male Wistar rats.Results: SD exacerbated weight loss, elevated corticosterone levels, ulcer area, and delayed GU healing. Ulcer+sleep-deprived rats exhibited increased thigmotaxis, reduced center exploration, locomotion, and rearing. Biochemical analyses revealed sustained malondialdehyde elevation and decreased antioxidant activity. Histology demonstrated progressive prefrontal cortex neurodegeneration, most severe in ulcer+sleep-deprived group by day 14. These findings demonstrates that SD during GU healing intensifies neuro-oxidative stress, disrupts antioxidant defenses, aggravates anxiety-like behavior and promotes neurodegeneration, collectively delaying gastric-mucosal repair.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13599305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148381705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Caring across cultures: a qualitative exploration of Macedonian carers supporting community-dwelling older adults in a large regional city in Australia. 跨文化关怀：在澳大利亚的一个大型区域城市马其顿照顾者支持社区居住的老年人的定性探索。

IF 1.7

BMC Research Notes Pub Date : 2026-07-02 DOI: 10.1186/s13104-026-07868-6

Jennifer White, Priscilla Viana Da Silva, Shingisai Chando, Ashley Young, Allison Boyes

{"title":"Caring across cultures: a qualitative exploration of Macedonian carers supporting community-dwelling older adults in a large regional city in Australia.","authors":"Jennifer White, Priscilla Viana Da Silva, Shingisai Chando, Ashley Young, Allison Boyes","doi":"10.1186/s13104-026-07868-6","DOIUrl":"10.1186/s13104-026-07868-6","url":null,"abstract":"Objective: Carers from culturally and linguistically diverse (CALD) backgrounds may experience challenges navigating healthcare systems that require English language proficiency. This study aimed to explore the experiences of carers supporting community-dwelling Macedonian older adults in a large regional city in Australia.Design: An interpretative qualitative study. Semi-structured interviews were conducted with informal carers of community-dwelling Macedonian older adults between August and November 2022. Data were analysed using an inductive thematic approach.Results: Participants ranged in age from 18 to 65 years and were predominantly adult children of the care recipient. Three overarching themes were identified: (1) persevering under the weight of ongoing interpreting responsibilities; (2) organising life around the demands of care; and (3) reconciling cultural expectations with the pressures of caregiving. Carers frequently assumed responsibility for interpreting during healthcare encounters, coordinating services, and navigating complex aged care systems. Many participants reported modifying employment and daily routines to accommodate caregiving responsibilities. Cultural expectations of family responsibility also influenced how carers understood and accepted their caregiving role.Conclusions: Family carers play a critical role in supporting older CALD adults to access healthcare and aged care services. However, gaps remain in the provision of culturally appropriate information, care coordination and interpreter support. Strengthening culturally responsive models of care and improving access to tailored service navigation support may help reduce caregiver burden and improve access to services for CALD communities.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13599250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148374723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and determinants of dyslipidemia among residents of Kurdistan Region of Iraq; a community-based study. 伊拉克库尔德斯坦地区居民血脂异常患病率及影响因素分析一项基于社区的研究。

IF 1.7

BMC Research Notes Pub Date : 2026-07-02 DOI: 10.1186/s13104-026-07941-0

Nawfal R Hussein, Dildar H Musa, Halder J Abozait, Majeed H Mustafa, Delovan S Mahfodh, Ibrahim A Naqid, Kavi M Sadoun, Shah V Ibrahim, Helan R Abdulrahman, Ahmed A Mosa, Mohammed D Younus

{"title":"Prevalence and determinants of dyslipidemia among residents of Kurdistan Region of Iraq; a community-based study.","authors":"Nawfal R Hussein, Dildar H Musa, Halder J Abozait, Majeed H Mustafa, Delovan S Mahfodh, Ibrahim A Naqid, Kavi M Sadoun, Shah V Ibrahim, Helan R Abdulrahman, Ahmed A Mosa, Mohammed D Younus","doi":"10.1186/s13104-026-07941-0","DOIUrl":"10.1186/s13104-026-07941-0","url":null,"abstract":"Objective: Cardiovascular diseases (CVD) remain the leading cause of global mortality, with dyslipidemia being a major modifiable risk factor. Dyslipidemia remains inadequately studied in developing countries like Iraq, so this study aims to assess the prevalence and determinants of dyslipidemia among residents of the Kurdistan Region of Iraq. This cross-sectional study was conducted between January and April 2025 and included 1132 participants aged ≥ 18 years. Dyslipidemia was defined based on prior confirmed diagnosis with documented laboratory, chronic disease registry identification card or specialist physician report.Results: The overall prevalence of dyslipidemia was 13.5%, lower than neighboring regions, with higher prevalence in men (15.8%) compared to women (9.9%). Significant determinants of dyslipidemia in multivariable logistics regression included low education (OR 3.197, 95% CI: 2.257-4.527), obesity (4.039, 2.464-6.619), hypertension (6.301, 4.205-9.44), and ischemic heart disease (11.21, 6.16-20.43). These findings underscore the need for public health initiatives focused on promoting healthy lifestyles, particularly in at-risk populations, to reduce the burden of dyslipidemia and prevent cardiovascular events.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13595650/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148374726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulation of brain-kidney crosstalk by olanzapine in aluminum chloride-induced memory impairment: a preclinical investigation. 奥氮平对脑肾串扰在氯化铝诱导记忆损伤中的调节作用：一项临床前研究。

IF 1.7

BMC Research Notes Pub Date : 2026-07-01 DOI: 10.1186/s13104-026-07940-1

Lawrence D Adedayo, Timothy O Emmanuel, Akinolu E Adeleke, Saminu Samaila, Gideon O Ayilara, Olubayode Bamidele, Gideon B Ojo, Nimedia G Aitokhuehi, Ovayoza O Mosugu, Iliya Ezekiel, Ejike D Eze

{"title":"Modulation of brain-kidney crosstalk by olanzapine in aluminum chloride-induced memory impairment: a preclinical investigation.","authors":"Lawrence D Adedayo, Timothy O Emmanuel, Akinolu E Adeleke, Saminu Samaila, Gideon O Ayilara, Olubayode Bamidele, Gideon B Ojo, Nimedia G Aitokhuehi, Ovayoza O Mosugu, Iliya Ezekiel, Ejike D Eze","doi":"10.1186/s13104-026-07940-1","DOIUrl":"10.1186/s13104-026-07940-1","url":null,"abstract":"Objective: The study investigated the impact of OLN on cognitive function, oxidative stress, neuroinflammation, and renal functions in a mouse model of aluminum chloride (AlCl₃) induced memory impairment. Twenty-five male mice were divided into five groups: group 1 (control) distilled water (10 mL/kg), group II AlCl₃ (100 mg/kg), group III AlCl₃ + OLN (0.5 mg/kg), group IV AlCl₃ + OLN (1.0 mg/kg), and group V AlCl₃ + Donepezil (1 mg/kg). Treatments were administered orally for seven days. Cognitive performance was assessed via Y-maze and open field test. Post-sacrifice, brain and kidney tissues were harvested and analysed for the levels of malondialdehyde (MDA), reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), tumour necrosis factor-alpha (TNF-α), nitrites, urea, and total protein, alongside histological examination of the hippocampus and prefrontal cortex.Results: AlCl₃ significantly impaired cognitive and locomotor performance, elevated MDA, TNF-α, nitrite, urea levels, and reduced GSH, CAT, SOD and total protein. Histological studies showed that AlCl3 caused neuronal loss in the hippocampus and prefrontal cortex. Findings of the study showed that OLN attenuated behavioural and biochemical changes in a dose-dependent manner, and at a higher dose preserved the neurons and enhanced memory and reduces neuroinflammation with effects to comparable to donepezil.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13591807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148367135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tagged and untagged amyloid precursor protein E2 domain have comparable thermal stability and metal-ion binding propensity. 标记和未标记淀粉样蛋白前体E2结构域具有相当的热稳定性和金属离子结合倾向。

IF 1.7

BMC Research Notes Pub Date : 2026-07-01 DOI: 10.1186/s13104-026-07938-9

Zamaxasibe Nxele, Sphamandla Ndlovu, Candice Ngubane, Raymond Hewer, Alexandré Delport

{"title":"Tagged and untagged amyloid precursor protein E2 domain have comparable thermal stability and metal-ion binding propensity.","authors":"Zamaxasibe Nxele, Sphamandla Ndlovu, Candice Ngubane, Raymond Hewer, Alexandré Delport","doi":"10.1186/s13104-026-07938-9","DOIUrl":"10.1186/s13104-026-07938-9","url":null,"abstract":"Objective: The amyloid precursor protein, central to the pathology of several disease including Alzheimer's disease, cancer and obesity, represents a therapeutic target. Being able to screen amyloid precursor protein, and its domains, in rapid high-throughput assays could advance drug discovery efforts. The purpose of this study was to determine if removal of a C-terminal tag from the amyloid precursor protein E2 domain was necessary for ligand screening using differential scanning fluorometry. To this end, we assessed both the thermal stability and metal-ion binding propensity in both the tagged and untagged protein.Results: The C-terminal myc-6xHis-tag does not impact the amyloid precursor protein E2 domain thermal stability with comparable melting temperatures of 59.4 °C and 59.1 °C in sodium phosphate buffer, for tagged and untagged protein, respectively. Additionally, the tag had no influence on the ability of the metal-ions, Zn2+ and Cu2+, to shift the thermal stability of the E2 domain indicating that metal-ion binding is not influenced by the tag. We therefore suggest that it is not necessary to remove the C-terminal tag from the E2 domain for ligand screening using differential scanning fluorometry.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13595551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148367070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0