BMC Research Notes最新文献

{"title":"Paradigm shift in age of multiple myeloma patients: a study from a tertiary care government oncology hospital in Pakistan.","authors":"Maryam Habib, Wardah Aslam, Saeeda Aziz","doi":"10.1186/s13104-025-07223-1","DOIUrl":"https://doi.org/10.1186/s13104-025-07223-1","url":null,"abstract":"<p><strong>Purpose: </strong>Multiple myeloma is a hematological disorder characterized by the presence of clonal plasma cells in the bone marrow that produce anemia, bone lesions, and kidney disease. The aim of this study was to determine the clinicopathological profile including age, sex, common symptoms and stage of patients with multiple myeloma diagnosed and managed in a government sector hospital in Pakistan.</p><p><strong>Patients and methods: </strong>This retrospective cross-sectional study was conducted over a period of eight years at the Institute of Nuclear Medicine, Oncology, and Radiotherapy in Islamabad. Of the 89 patients diagnosed and treated in this hospital, 81 patients with complete data were included in the study.</p><p><strong>Results: </strong>Of the 81 patients, 55 were males and 27 were female patients. The mean age at diagnosis in our set of patients was 53 years, with 79% of the patients aged ≤ 60 years of age. The most common presenting symptom was bone pain followed by generalized fatigue and infections. The most common monoclonal gammopathy was IgG;12.3% patients were in stage I, 48.1% in stage II and 39.5% in stage III.</p><p><strong>Conclusions: </strong>Our study revealed that the patients in our institution presented multiple myeloma at much younger age than the patients seen in developed countries of the world. Most studies from Pakistan present data from private sector hospitals. This is the first study conducted at a Pakistani government hospital in patients with multiple myeloma. More studies should be conducted representing patients from both private and government hospitals to understand the true magnitude of the disease and the factors behind this age difference in patients with multiple myeloma in our part of the world.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"147"},"PeriodicalIF":1.6,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A cross-sectional survey of poultry management systems, practices and antimicrobial use in relation to disease outbreak in Pakistan.","authors":"Farrukh Saleem, Aqsa Ameer, Farhan Afzal, Muhammad Usman, Hamid Irshad, Sadia Sattar, Umer Zeeshan Ijaz, Sundus Javed","doi":"10.1186/s13104-025-07220-4","DOIUrl":"https://doi.org/10.1186/s13104-025-07220-4","url":null,"abstract":"<p><strong>Objectives: </strong>The study aimed to examine how management practices, farming setup and breed influence disease outbreaks. It also sought to investigate the frequency and types of antimicrobials used, as well as the relationship between antimicrobial usage and disease occurrences.</p><p><strong>Methods: </strong>We conducted a survey of 140 poultry farms [Broiler farms = 66; Layer farms = 36; Local (Desi and its crosses) farms = 38] across major poultry producing regions of Pakistan. The gathered information covered demographics as well as the farming associated parameters including size, type of the farms, management practices, breeds raised, disease outbreak and antimicrobials use.</p><p><strong>Results: </strong>Using contingency analyses and log binomial regression models, we identified Broiler control sheds at high risk of disease. Diseases such as Avian Influenza, Newcastle Disease, and Fowl Typhoid were frequently reported and their outbreaks were associated with low cleaning frequency, high stocking density, bedding material using rice husk, and canola as a major feed ingredient. Farmer education was associated with a decrease in disease outbreak. Antimicrobial use was associated with farming experience, farm size, type and breed.</p><p><strong>Conclusion: </strong>High disease incidence is associated with management practices and breed types across various farm setups. Experienced Broiler farmers often report disease outbreaks and use antimicrobials more frequently. Educated farmers, however, experience fewer outbreaks and can better regulate antimicrobial usage.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"144"},"PeriodicalIF":1.6,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Denosumab for treating periprosthetic osteolysis: a feasibility study.","authors":"Michael Axenhus, Henrik Bodén, Paula Kelly-Pettersson, Olof Sköldenberg","doi":"10.1186/s13104-025-07216-0","DOIUrl":"https://doi.org/10.1186/s13104-025-07216-0","url":null,"abstract":"<p><strong>Objective: </strong>Wear-induced osteolysis is a leading cause of late failure in total hip arthroplasty (THA). Denosumab, a RANKL inhibitor, suppresses osteoclast activity and may slow osteolytic progression. This feasibility study aimed to assess the practicality of conducting a randomized, double-blind, placebo-controlled trial evaluating Denosumab's effect on periprosthetic osteolysis in asymptomatic THA patients.</p><p><strong>Results: </strong>Twelve patients were enrolled; ten completed follow-up. No significant difference in lesion volume change was observed between groups (Denosumab: +1.53 cm³; Placebo: +0.49 cm³). Secondary clinical outcomes also showed no notable differences. The trial protocol, recruitment, treatment, and follow-up were feasible, though slow enrollment limited statistical power. This study demonstrates the feasibility of a larger trial investigating Denosumab for osteolysis prevention.</p><p><strong>Trial registration: </strong>Clinicaltrails.gov, NCT02299817. Registered 20 November 2014. https://www.</p><p><strong>Clinicaltrials: </strong>gov/study/NCT02299817?term=Denosumab%20for%20Treating%20Periprosthetic%20Osteolysis.%26;rank=1.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"151"},"PeriodicalIF":1.6,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mother: a maternal online technology for health care dataset.","authors":"Odongo Steven Eyobu, Brian Angoda Nyanga, Lukman Bukenya, Daniel Ongom, Tonny J Oyana","doi":"10.1186/s13104-025-07230-2","DOIUrl":"https://doi.org/10.1186/s13104-025-07230-2","url":null,"abstract":"<p><strong>Objectives: </strong>These data enable the development of both textual and speech based conversational machine learning models that can be used by expectant mothers to provide answers to challenges they face during the different trimesters of their pregnancy. Such models are key to the improvement of the lives of pregnant mothers, specifically in low resourced settings where doctors advise is limited by access to hospitals and language barrier. These data were used to develop a conversational chatbot model tailored for mothers in their first, second and third trimesters of pregnancy.</p><p><strong>Data description: </strong>503 question and answer pairs on maternal health were collected through a survey of challenges facing pregnant mothers in a rural and semi-urban area of Uganda. The answers to the questions were provided and validated by professional medical personnel. The participants were purposively sampled, focusing on women in their 1st, 2nd and 3rd trimesters, with a 94% response rate. The dataset addresses common health concerns, symptoms, and conditions associated with pregnancy, particularly for women without immediate access to medical personnel. It targets maternal health outcomes such as pregnancy, morbidity, and mortality, specifically among women of reproductive age.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"150"},"PeriodicalIF":1.6,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro comparative effects of alcohol-containing and alcohol-free mouthwashes on surface roughness of bulk-fill composite resins.","authors":"Sepideh Ayatollahi, Aryan Davoudi, Hooman Momtazi","doi":"10.1186/s13104-025-07213-3","DOIUrl":"https://doi.org/10.1186/s13104-025-07213-3","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to compare the effects of alcohol-containing and alcohol-free mouthwashes on the surface roughness of bulk-fill composite resins. In this in-vitro, experimental study, 60 composite specimens measuring 6 mm in diameter and 2 mm in height were fabricated from Tetric N-Ceram and X-tra fil composite resins using a stainless-steel mold. After curing for 20 s, the specimens were immersed in distilled water and incubated at 37 °C for 24 h. Baseline roughness was measured before dividing them into three groups for immersion in water, alcohol-containing, or alcohol-free Listerine for 24 h, simulating two years of use. The specimens were then dried at room temperature, and their surface roughness was measured again. Data was analyzed by two-way ANOVA and t-test (α = 0.05).</p><p><strong>Results: </strong>No significant change occurred in surface roughness of specimens after immersion in the respective solutions (P > 0.05). The type of composite and the type of solution had no significant effect on the surface roughness of specimens (P > 0.05). The results showed that Listerine alcohol-containing and alcohol-free mouthwashes had no significant effect on the surface roughness of the tested bulk-fill composite resins and no significant difference with each other in this respect.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"146"},"PeriodicalIF":1.6,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An improved toolkit of gateway- and gibson assembly-compatible vectors for protoplast transfection and agrobacterium-mediated plant transformation.","authors":"Jeffrey R Allen, Michael Flanagan, Sunita Pathak, Samantha K Emenecker, Ryan J Emenecker, Lucia C Strader","doi":"10.1186/s13104-025-07231-1","DOIUrl":"https://doi.org/10.1186/s13104-025-07231-1","url":null,"abstract":"<p><strong>Objective: </strong>Understanding the regulation and function of plant genes is essential for addressing the challenges faced by modern agriculture. Plant transformation, in conjunction with fluorescence microscopy, offers a powerful approach to investigate the dynamic behavior of plant genes and the proteins they encode. We previously developed a set of Gateway-compatible tissue-specific plant transformation vectors. In this paper we aim to expand the toolkit of vectors available for Agrobacterium-mediated plant transformation and protoplast transfection.</p><p><strong>Results: </strong>Here, we introduce new Agrobacterium-mediated plant transformation vectors by introducing additional fluorophores to create the pJRA vector series. Additionally, we introduce the pLCS series of vectors, a new set of modular Gateway- and Gibson assembly-compatible vectors designed for protoplast transfection. All described vectors are available from Addgene to serve as a resource for the plant research community.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"149"},"PeriodicalIF":1.6,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Results of the preclinical multicenter randomized controlled paclitaxel-induced neuropathy prevention replication study (PINPRICS).","authors":"Wolfgang Boehmerle, Tim Hagenacker, Markus Leo, Linda-Isabell Schmitt, Helmar C Lehmann, Ines Klein, Regina Stegherr, Frank Konietschke, Matthias Endres, Petra Huehnchen","doi":"10.1186/s13104-025-07206-2","DOIUrl":"https://doi.org/10.1186/s13104-025-07206-2","url":null,"abstract":"<p><strong>Objective: </strong>Chemotherapy-induced peripheral neuropathy (CIPN) is a frequent and serious side effect of many cytotoxic drugs, including paclitaxel. Despite the identification of treatment options in animal models, clinical trials for the treatment or prevention of CIPN have been negative. Major challenges for successful clinical translation of preclinical data include a lack of reproducibility and randomization, small sample sizes and insufficient statistical tests. We therefore conducted a confirmatory, preclinical multicenter randomized controlled replication trial to test the safety and efficacy of three drugs for preventing paclitaxel-induced polyneuropathy: (1) nilotinib, (2) lithium carbonate and (3) interleukin-6-neutralizing antibodies. We preregistered the data analysis plan as well as the two-step study protocol: the optimal doses of the three compounds were assessed first and then tested in a mouse breast cancer xenograft model to compare safety and efficacy.</p><p><strong>Results: </strong>Unfortunately, toxicity of intraperitoneally administered nilotinib in combination with paclitaxel was observed, and higher-than-expected tumor growth resulted in a lack of power when the trial was analyzed. Thus, although lithium carbonate and IL-6-neutralizing antibodies tended toward neuroprotection, the differences between these groups were not statistically significant. However, the PINPRICS study ultimately still provides important lessons with regard to the planning and conduction of multicenter preclinical trials.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"145"},"PeriodicalIF":1.6,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The immunosuppressive role of neutrophils in infectious and oncological conditions: A study of chemokine receptor CXCR3 and human neutrophil lipocalin levels.","authors":"Khuntsaria Irakli, Kldiashvili Ekaterina, Krajacic Mladen","doi":"10.1186/s13104-025-07229-9","DOIUrl":"https://doi.org/10.1186/s13104-025-07229-9","url":null,"abstract":"<p><strong>Background: </strong>Neutrophils are key players in the innate immune system, responsible for rapid responses to infections through mechanisms such as phagocytosis and the release of reactive oxygen species (ROS). Beyond their role in host defense, neutrophils also contribute to the pathogenesis of various diseases, including infections, metabolic disorders, autoimmune diseases, and cancer. Understanding the immunosuppressive role of neutrophils, particularly through markers like human neutrophil lipocalin (HNL) and the chemokine receptor CXCR3, is crucial for developing targeted therapeutic strategies.</p><p><strong>Materials and methods: </strong>This study involved 200 participants divided into four groups: 50 patients with acute respiratory infection, 50 COVID-19 recovered patients, 50 oncology patients, and 50 healthy donors as controls. Peripheral blood samples were collected and analyzed using enzyme-linked immunoassay (ELISA) to quantify levels of HNL and CXCR3. Data were analyzed using SPSS version 25.0, employing descriptive statistics, the Shapiro-Wilk test for normality, one-way ANOVA for normally distributed variables, and the Kruskal-Wallis test for non-normally distributed variables. Post-hoc comparisons were conducted using Tukey's HSD and Dunn's tests.</p><p><strong>Results: </strong>CXCR3 levels were stable across groups, with no significant differences found. Acute respiratory infection patients had an average CXCR3 level of 150 ± 20 pg/ml, while COVID-19 recovered patients had slightly lower levels at 140 ± 18 pg/ml. Oncology patients had elevated CXCR3 levels at 160 ± 22 pg/ml, similar to healthy donors at 150 ± 19 pg/ml. HNL levels varied more, with COVID-19 recovered patients showing notably lower levels (100 ± 12 ng/ml) compared to other groups. Oncology patients exhibited higher HNL levels, especially those with prostate cancer (150 ± 20 ng/ml).</p><p><strong>Conclusion: </strong>The findings highlight the consistent expression of CXCR3 across various conditions, making it a reliable marker for immune response assessment. The distinct HNL profiles, particularly the lower levels in COVID-19 recovered patients and higher levels in prostate cancer patients, suggest unique neutrophil activities and immune responses. These insights into neutrophil-mediated immunosuppression and inflammation could inform the development of targeted therapies for infections, cancer, and autoimmune diseases. Further research is needed to elucidate the specific mechanisms underlying neutrophil-induced immunosuppression.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"148"},"PeriodicalIF":1.6,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The antibiofilm potential of Clidemia hirta against Candida albicans: preliminary study.","authors":"Sufi Desrini","doi":"10.1186/s13104-025-07209-z","DOIUrl":"10.1186/s13104-025-07209-z","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to screen the activity of ethanol extract of Clidemia hirta fruits and leaves against 24 h old Candida albicans biofilm by measuring cell viability with 3-(4,5-dimethylthiazol- 2-yl)- 2,5-diphenyl tetrazolium bromide (MTT) and planktonic cells of C. albicans using the broth microdilution method.</p><p><strong>Results: </strong>The ethanol extract of C. hirta fruits inhibited (50%) the C. albicans biofilm at a concentration fourfold higher than those required to inhibit planktonic cells (31.25 µg/mL vs. 7.8 µg/mL). This finding is supported by the morphological changes of C. albicans biofilm under scanning electron microscopy (SEM) visualization. A total of 55 compounds were detected using UHPLC coupled with Thermo Scientific™ Q Exactive™ High-Resolution Mass Spectrometer (HRMS). The top three compounds of the ethanol extract: dodecyl sulfate, bis(2-ethylhexyl) phthalate, and erucamide) might be responsible compounds for the antibiofilm activity of ethanol extract of C. hirta.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"143"},"PeriodicalIF":1.6,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between Trichomonas vaginalis infection and spontaneous preterm labour in Lagos, Nigeria: an analytical cross-sectional study.","authors":"Oyebola H Sunmonu, Kehinde S Okunade, Omololu Adegbola","doi":"10.1186/s13104-025-07196-1","DOIUrl":"10.1186/s13104-025-07196-1","url":null,"abstract":"<p><strong>Background: </strong>Preterm labour (PTL) leading to preterm birth is a grave complication of pregnancy that attracts long-term medical and social sequelae. However, there is still a paucity of data on the impact of Trichomonas vaginalis infection on PTL especially among women in sub-Saharan Africa.</p><p><strong>Objective: </strong>This study determined the association between T. vaginalis infection and PTL among pregnant women in Lagos, Southwest, Nigeria using a molecular-based rapid antigen detection technique to diagnose T. vaginalis infection.</p><p><strong>Methods: </strong>This was a multicenter analytical cross-sectional study carried out among parturients managed in the labour ward units of three hospitals in Lagos, Southwest Nigeria between April and December 2019. High vaginal swab samples were collected from n = 105 pregnant women with PTL and an equal number of women who had term labour between April and December 2019. T. vaginalis Antigen Rapid test was used for the detection of T. vaginalis in the vaginal samples. Multivariable binary logistic regression analyses were used to control for all potential confounders in the association between T. vaginalis infection and PTL. Statistical significance was reported at p < 0.05.</p><p><strong>Results: </strong>The prevalence of T. vaginalis infection was significantly higher in women with PTL compared to those with term labour (12.4% vs. 2.9%, p = 0.009). On adjustments for age, parity, booking and educational status, abnormal vaginal discharge and occurrence of prelabour rupture of membranes, there was no association between T. vaginalis infection and PTL (adjusted Odds ratio = 0.94, 95% CI: 0.10-9.29).</p><p><strong>Conclusions: </strong>The study showed no independent association between T. vaginalis and PTL. We recommend future adequately powered longitudinal studies with consideration for other potential confounding factors, such as sexual lifestyle and STIs, to further explore any potential relationship between T. vaginalis and PTL.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"142"},"PeriodicalIF":1.6,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}