Elisa Fernández-Descalzo, Lorena Álvarez-Iglesias, Ana Butrón, Serafín J González-Prieto
{"title":"Influence of Sesamia nonagrioides and Fusarium verticillioides on carbon elemental and isotopic composition in maize stem piths.","authors":"Elisa Fernández-Descalzo, Lorena Álvarez-Iglesias, Ana Butrón, Serafín J González-Prieto","doi":"10.1186/s13104-025-07278-0","DOIUrl":"https://doi.org/10.1186/s13104-025-07278-0","url":null,"abstract":"<p><p>The stem borer Sesamia nonagrioides and the fungus Fusarium verticillioides are frequently present in maize. However, their interaction with this crop and their effects on its physiology remain poorly understood. This study explores the combined impact of these two organisms on carbon composition and δ¹³C content in the stem pith. Eight genetically distinct maize lines (A239, A509, A630, A637, EP42, EP77, EP125, PB130) were subjected to four treatments: an untreated control (UC), infection by F. verticillioides (FV), attack by S. nonagrioides (SN), and infection by F. verticillioides and infestation by S. nonagrioides (FS). Results show moderate variation in δ¹³C values between UC genotypes (-12.6 to -13.1‰, with the exception of line EP77 at -13.8‰), with minor differences related to their inbred resistance to the insect and fungus. Insect infestation results in a noticeable reduction in δ¹³C (0.6‰), but this decrease is partially attenuated in plants previously colonized by the fungus, suggesting an influence of the fungus on the maize's defensive response. In addition, the analysis of <sup>13</sup>C isotopic composition and carbon signatures provide key elements for a better understanding of maize defence mechanisms. To our knowledge, this study is the first to explore the impact of Sesamia and Fusarium, and their interaction, on carbon metabolism in different maize genotypes.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"219"},"PeriodicalIF":1.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Measuring and visualizing scattered x-rays using red-green-blue (RGB) heat maps during extracorporeal membrane oxygenation in the intensive care unit.","authors":"Tomihiko Daioku, Fumiko Oishi, Masanao Kobayashi, Naoko Arakawa, Harumi Ejiri","doi":"10.1186/s13104-025-07286-0","DOIUrl":"https://doi.org/10.1186/s13104-025-07286-0","url":null,"abstract":"<p><strong>Objective: </strong>This study investigated the continuous risks of scattered radiation associated with a series of procedures-from extracorporeal membrane oxygenation (ECMO) cannulation through to enteral feeding tube insertion-in an intensive care unit (ICU) setting by visualizing the dose distribution using a red-green-blue (RGB) color map.</p><p><strong>Results: </strong>The scattered radiation doses were measured at 80 points around a tissue-equivalent phantom using calibrated nanoDot dosimeters, and an RGB color map was generated to visualize the dose intensity. Radiation doses near the X-ray source reached 783.6 µSv/hour at 50 cm, exceeding annual public exposure limits within 1 h. These findings emphasize the importance of using appropriate radiation protection equipment, suggesting that distance is a key factor in reducing the ICU exposure risk. They also offer practical guidance for planners involved in radiation safety management in hospital settings.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"220"},"PeriodicalIF":1.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Barriers to home-based physical activity and predictors of activity levels among women with high sedentary habits: an explanatory mixed-methods study.","authors":"Malihe Noori-Sistani, Hamid Allahverdipour, Mohammad Vahedian-Shahroodi, Mahta Eskandarnejad, Nahid Ashkriz, Zeinab Javadivala","doi":"10.1186/s13104-025-07243-x","DOIUrl":"10.1186/s13104-025-07243-x","url":null,"abstract":"<p><strong>Objective: </strong>Understanding barriers to home-based physical activity is crucial for developing strategies to increase participation among women with high sedentary habits. This study aims to comprehensively scrutinize these barriers and predictor factors of physical activity levels, utilizing an explanatory mixed methods design. The quantitative phase involved 537 women, who completed a researcher-made questionnaire and the International Physical Activity Questionnaire (IPAQ) to assess physical activity levels, predictor factors, and barriers. The qualitative phase engaged 12 participants through in-depth interviews to gain a deeper understanding of barriers. Chi-square statistical tests, multinomial logistic regression, and content analysis were used.</p><p><strong>Results: </strong>83.8% of women were in the inactive and low-activity category. The main barriers to physical activity included not prioritizing exercise, insufficient time, household responsibilities, and a deficit in motivation. Predictors included environmental barriers (P = 0.009, β = 0.701), social obstacles (P ≤ 0.001, β = 1.179), and lack of motivation (P ≤ 0.001, β = 1.836), all of which significantly impact the level of moderate to vigorous physical activity. The qualitative analysis classified barriers into three categories: personal, social, and environmental. Interventions such as community programs, awareness campaigns, and improved infrastructure are crucial. Health policymakers can take action with targeted strategies to remove barriers and promote women's physical activity.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"217"},"PeriodicalIF":1.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12079843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nastiti Intan Permata Sari, Putu Yuliandari, Ajeng Kusumaningtyas Pramono, Dwi Tyastuti, Abdul Hadi Furqoni, Fitriana Fitriana, Sunarno Sunarno, Dewi Setyowati, Ni Luh Putu Indi Dharmayanti
{"title":"Genomic dataset of multidrug-resistant Klebsiella quasipneumoniae from Indonesia.","authors":"Nastiti Intan Permata Sari, Putu Yuliandari, Ajeng Kusumaningtyas Pramono, Dwi Tyastuti, Abdul Hadi Furqoni, Fitriana Fitriana, Sunarno Sunarno, Dewi Setyowati, Ni Luh Putu Indi Dharmayanti","doi":"10.1186/s13104-025-07269-1","DOIUrl":"10.1186/s13104-025-07269-1","url":null,"abstract":"<p><strong>Objectives: </strong>Klebsiella pneumoniae is a free-living bacterium found anywhere, including soil, water, and various types of plants, animals, and humans. Due to identical biochemical test results, Klebsiella quasipneumoniae, a member of the Klebsiella pneumoniae complex, is often misidentified as Klebsiella pneumoniae. This distinct species can be accurately identified solely through whole-genome sequencing. This bacterium poses a significant public health threat due to its increasing antibiotic resistance, ability to cause severe disease, and potential for community-acquired and hospital-acquired infections. However, there was no previous report of K. quasipneumoniae from Indonesia. Subsequent research focusing on antimicrobial-resistant gene analysis, virulence determinants, evolutionary relationship, and transmission pathways based on this dataset will enhance understanding of this species and their differences with other Klebsiella pneumoniae complex organisms.</p><p><strong>Data description: </strong>We present a whole genome sequencing of four Klebsiella quasipneumoniae isolated from hospital wastewater in Jakarta, Indonesia. Initial bacterial identification was conducted which showed Klebsiella pneumoniae. However, the whole genome Average Nucleotide Identity (wgANI) was found to be Klebsiella quasipneumoniae. The genome size of Klebsiella quasipneumoniae I1-1, I1-3, I5-2, and I5-3 isolates were 5.4 Mb (GC = 57.72%), 5.5 Mb (GC = 57.72%), 5.4 Mb (GC = 57.73%), and 5.5 Mb (GC = 57.72%), respectively. Sequence data has been deposited in the GenBank database.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"218"},"PeriodicalIF":1.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12083092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nazma Sultana Lupin, Birgit Fritz, Siegfried Wahl, Markus Egert
{"title":"Using ATP measurements to rapidly evaluate the cleanliness of spectacle surfaces.","authors":"Nazma Sultana Lupin, Birgit Fritz, Siegfried Wahl, Markus Egert","doi":"10.1186/s13104-025-07282-4","DOIUrl":"https://doi.org/10.1186/s13104-025-07282-4","url":null,"abstract":"<p><strong>Objective: </strong>Microbial contamination on used spectacles poses a potential health risk to spectacle wearers and people regularly working with worn spectacles, such as opticians. ATP measurement is widely used to evaluate the cleanliness of surfaces based on the detection of adenosine triphosphate, a molecule found in and around living cells. In this study, we investigated the suitability of this method to rapidly and easily evaluate the efficacy of different cleaning methods for spectacles. Additionally, we examined the correlation between ATP content on spectacle surfaces with aerobic and anaerobic bacterial colony counts. We swab-sampled worn spectacles and used an ATP bioluminescence assay to assess the level of cellular contamination.</p><p><strong>Results: </strong>Six cleaning methods were tested on ten worn spectacles each, and ATP levels were correlated with both aerobic and anaerobic bacterial counts. All investigated cleaning methods showed a significant median reduction of the ATP content on spectacle surfaces, between 75 and 93%. Germ counts and ATP levels showed no significant correlation for aerobic but for anaerobic cultivation. Higher anaerobic germ counts correlated positively with higher ATP levels. ATP measurement is a suitable method to rapidly and easily demonstrate the efficacy of cleaning measures for spectacle surfaces also under non laboratory conditions.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"216"},"PeriodicalIF":1.6,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12080132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A qualitative study on preparedness requirements for responding to chemical, biological, radiological, and nuclear incidents in Iran.","authors":"Hamid Karimi Kivi, Vahid Hadi, Saeed Nazari, Peyman Namdar, Mahboobeh Afzali","doi":"10.1186/s13104-025-07272-6","DOIUrl":"10.1186/s13104-025-07272-6","url":null,"abstract":"<p><strong>Background and purpose: </strong>The sudden increase in the number of exposed individuals, diverse injuries, damage to health equipment, and disruptions in health services highlight the need for a structured response to radiological and nuclear incidents. This study aims to identify the requirements for responding to CBRN incidents in Iran. This research contributes to a deeper understanding of the complexities involved in CBRN preparedness and response in Iran, highlighting areas for potential improvement and policy development.</p><p><strong>Methodology: </strong>This qualitative research conducted in 2023 involved semi-structured interviews with 32 specialists selected through purposive sampling until data saturation was reached. Thematic analysis was employed to analyze the data.</p><p><strong>Findings: </strong>The requirements for responding to CBRN incidents were categorized into three main classes with nine subcategories: TRAINING: Effective training content, establishment of specialized training centers, identification of target audiences, educational needs assessment, and continuous education.</p><p><strong>Drills: </strong>Implementation of various operational drills and their content.</p><p><strong>Emergency response planning: </strong>Elements of response plans and evaluation of these plans.</p><p><strong>Conclusion: </strong>Timely and appropriate responses from the health system during critical phases after such incidents are crucial for mitigating adverse health effects. This study provides insights into the necessary requirements for enhancing readiness in the health sector as Iran progresses towards adopting modern technologies.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"214"},"PeriodicalIF":1.6,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070526/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143976223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Distribution of polycystic ovary syndrome (PCOS) phenotypes in Iranian women: a cross-sectional study.","authors":"Asma Kheirollahi, Hediyeh Hamidi, Akram Vatannejad","doi":"10.1186/s13104-025-07202-6","DOIUrl":"10.1186/s13104-025-07202-6","url":null,"abstract":"<p><strong>Objective: </strong>Polycystic ovary syndrome (PCOS) is a heterogeneous disorder characterized by diverse clinical and metabolic manifestations. This study aimed to investigate the prevalence of PCOS phenotypes and their association with hematological, biochemical, and hormonal parameters in PCOS, with a particular focus on infertile women and those with recurrent pregnancy loss (RPL).</p><p><strong>Results: </strong>Phenotype A was the most prevalent phenotype overall and within both infertile and RPL subgroups. However, no significant differences in hematological, biochemical, or hormonal parameters were observed among the PCOS phenotypes, except for lower RBC and hematocrit levels in phenotype F. PCOS women with RPL demonstrate significantly lower levels of RBC, hemoglobin, and hematocrit in phenotype F.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"215"},"PeriodicalIF":1.6,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12076859/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ebenezer Mensah Gyimah, Ebenezer Dassah, Maxwell Peprah Opoku, Wisdom Kwadwo Mprah, William Nketsia, Afua Ntoaduro, Philip Atta Mensah, Cecilia Opoku, Clement Osei Tutu, Issaka Zakia, Richard Adade, Paul Okyere
{"title":"Consumers' recommendations for improving access to healthcare services to adolescents with disabilities in Ghana.","authors":"Ebenezer Mensah Gyimah, Ebenezer Dassah, Maxwell Peprah Opoku, Wisdom Kwadwo Mprah, William Nketsia, Afua Ntoaduro, Philip Atta Mensah, Cecilia Opoku, Clement Osei Tutu, Issaka Zakia, Richard Adade, Paul Okyere","doi":"10.1186/s13104-025-07267-3","DOIUrl":"10.1186/s13104-025-07267-3","url":null,"abstract":"<p><strong>Introduction: </strong>In Ghana, Adolescents with mobility and visual impairments and their families, encounter healthcare disparities, including socio-economic, physical, structural and attitudinal challenges, compared to the general population. Nonetheless, there is limited motivation to understand how to improve healthcare access for these marginalized consumers (i.e., adolescents with disabilities and their families). Consequently, the study explored marginalized consumers' recommendations for improving access to healthcare services, described in Ghana's Act 715. Informed by the qualitative descriptive design, forty-five (45) participants were purposively recruited and interviewed, adopting a semi-structured interview guide. Data gathered were subjected to content analysis and interpreted via lenses of the theory of dimensions of healthcare access.</p><p><strong>Results: </strong>The study's findings were layered within areas of improving policy-practice interphase. Consumers called on policy makers to restructure the NHIS to cover indirect costs of accessing healthcare services for adolescents with disabilities. In respect of improving practice, it was also suggested that training and education on disability phenomenon must be extended to all cadres of healthcare workers including administrative staff, who provided services to this marginalized social unit. Participants further recommended enhancing the availability of healthcare workers and services as well as ensuring respect and protection of human rights within healthcare facilities.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"213"},"PeriodicalIF":1.6,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070534/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143976030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The investigation of apoptosis-related genes in periodontitis.","authors":"Arezou Sayad, Fatemeh Hashemian, Leila Gholami, Masoud Jamali, Elham Badrlou, Saba Sadeghpour, Naghme Nazer, Sheyda Khalilian, Soudeh Ghafouri-Fard","doi":"10.1186/s13104-025-07274-4","DOIUrl":"10.1186/s13104-025-07274-4","url":null,"abstract":"<p><strong>Objectives: </strong>This study aims at determination of the roles of five apoptosis-related genes, namely CASP2, CASP8, BCL2, HULC and PVT1 in the pathoetiology of periodontitis via measurement of their expressions in both peripheral blood and tissues of affected persons.</p><p><strong>Results: </strong>CASP2 was over-expressed in gingiva of patients compared with healthy subjects (RME = 24.56, P < 0.001), and in both affected females and males (RME = 30.53, P = 0.03 and RME = 20.59, P = 0.01, respectively). BCL2 was higher in affected tissues compared with controls (RME = 32.28, P < 0.001) and in affected tissues of males versus controls (RME = 69.03, P < 0.001). Finally, HULC had lower level in the blood of patients (RME = 0.21, P = 0.01) and in the blood of female patients compared with normal females (RME = 0.15, P = 0.01). Other comparisons yielded no significant results. BCL2 and CASP2 had the highest diagnostic values for separation of diseased gingival tissues from normal ones. HULC has the best values in the distinction of blood samples of affected persons from control persons. Combination of transcript levels of CASP2, CASP8, BCL2, HULC and PVT1 changed AUC to 0.84 and 0.72 in tissues and blood samples, respectively. To conclude, these genes might be regarded as putative contributors in the pathophysiology of periodontitis.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"211"},"PeriodicalIF":1.6,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Henna Budhwani, Ibrahim Yigit, Hoa L Nguyen, Josh Bruce, C Lily Bond, Andrea Johnson
{"title":"Minority adolescents' HIV PrEP awareness and preferences in Alabama.","authors":"Henna Budhwani, Ibrahim Yigit, Hoa L Nguyen, Josh Bruce, C Lily Bond, Andrea Johnson","doi":"10.1186/s13104-025-07273-5","DOIUrl":"10.1186/s13104-025-07273-5","url":null,"abstract":"<p><strong>Objective: </strong>In the southern United States, adolescents are at elevated risk for HIV acquisition. In Alabama, school-based sexual health and HIV prevention education is strictly regulated and does not address the unique needs of sexual and gender minority (SGM) teenagers. To inform public health efforts, we assessed SGM adolescents' HIV prevention pre-exposure prophylaxis (PrEP) awareness and modality preferences by gender, race, and ethnicity.</p><p><strong>Results: </strong>Survey data were collected in 2023-2024 from SGM adolescents aged 14-17 years, lived in Alabama, with male sex assigned at birth (N = 206). Recruitment occurred online and in-person at a charter school. Data were analyzed using chi-squares and analysis of variance, controlling for age. Over half of respondents were sexually active, but only 26% had ever been tested for HIV. Half knew about PrEP. Of those with PrEP awareness, 41.9% were aware of daily pills; 32.3% of long-acting injectable PrEP. Pill-based PrEP was highly endorsed. Trans- and gender-non-conforming adolescents reported a greater preference for on-demand pill-based PrEP compared to men who have sex with men (p =.01). Non-Hispanics had a greater preference for a 6-month long-acting injectable option compared to Hispanic adolescents (p =.04). Findings suggest the need for HIV prevention interventions tailored to southern contexts and adolescent knowledge.</p><p><strong>Trial registration: </strong>Not Applicable.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"210"},"PeriodicalIF":1.6,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12067670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}