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Integration of Collaborative Cross and Hybrid Mouse Diversity Panel studies does not significantly improve genome wide association study results. 整合协作杂交和杂交小鼠多样性小组研究并没有显著改善全基因组关联研究结果。
IF 1.7
BMC Research Notes Pub Date : 2026-10-13 DOI: 10.1186/s13104-026-08067-z
Christoph D Rau
{"title":"Integration of Collaborative Cross and Hybrid Mouse Diversity Panel studies does not significantly improve genome wide association study results.","authors":"Christoph D Rau","doi":"10.1186/s13104-026-08067-z","DOIUrl":"10.1186/s13104-026-08067-z","url":null,"abstract":"<p><p>Genome-wide association studies rely on maximizing cohort size and minimizing environmental noise to increase their efficacy. Model organism Genetic Reference Populations offer significantly reduced environmental noise and deep phenotyping, yet rarely are multiple populations merged together. We evaluated whether combining the Collaborative Cross (CC) and Hybrid Mouse Diversity Panel (HMDP), two well-studied murine Genetic Reference Populations would improve GWAS outcomes. Utilizing two prior studies by our group that had performed the same study for heart failure in each cohort, we examined each cohort individually and then together using the pyLMM GWAS algorithm. Surprisingly, the combined cohort of 175 strains produced only 123 loci, a modest gain over the 104-strain HMDP (114), and fewer than in the 71-strain CC (149) and did not systematically improve resolution. Locus overlap between the CC and HMDP cohorts was very sparse, with only a single locus (near Eprs1) found to be significant in each cohort, although nearly half of all combined loci were also found in the CC cohort. These results caution that merging divergent GRPs may act to dilute signal and complicate interpretation, despite larger sample sizes.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"19 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149179459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The role of men's self-efficacy and women's reports in predicting biochemical exposure to secondhand smoke during pregnancy: a cross-sectional study. 男性自我效能感和女性报告在预测怀孕期间二手烟生化暴露中的作用:一项横断面研究。
IF 1.7
BMC Research Notes Pub Date : 2026-10-05 DOI: 10.1186/s13104-026-07959-4
Zohreh Karimiankakolaki, SeyedSaeed MazloomyMahmoodabad, Ashraf Kazemi
{"title":"The role of men's self-efficacy and women's reports in predicting biochemical exposure to secondhand smoke during pregnancy: a cross-sectional study.","authors":"Zohreh Karimiankakolaki, SeyedSaeed MazloomyMahmoodabad, Ashraf Kazemi","doi":"10.1186/s13104-026-07959-4","DOIUrl":"10.1186/s13104-026-07959-4","url":null,"abstract":"<p><strong>Objective: </strong>Exposure to secondhand smoke (SHS) during pregnancy poses serious health risks. This study investigated the relationships between smoking men's self-efficacy, their cigarette consumption, their pregnant spouses' reported exposure, and salivary cotinine levels as an objective biomarker of SHS exposure.</p><p><strong>Results: </strong>This cross-sectional study included 140 smoking men and their pregnant wives in Isfahan, Iran. Mean (SD) men's self-efficacy was 37.86 (4.27), cigarette consumption was 6.46 (4.50) cigarettes/day, reported exposure was 1.83 (1.82) cigarettes/day, and salivary cotinine was 52.90 (33.39) pg/ml. Significant correlations were observed between self-efficacy and cigarette consumption (r = -0.31), self-efficacy and reported exposure (r = -0.41), self-efficacy and cotinine (r = -0.27), and between reported exposure and cotinine (r = 0.72). In the adjusted regression model, women's reported exposure (β = 0.63, p < 0.001) and men's self-efficacy (β = -0.14, p = 0.022) independently explained 56% of the variance in salivary cotinine. Men with higher education and non-manual occupations exhibited significantly higher self-efficacy and lower exposure outcomes. Higher men's self-efficacy was associated with lower cigarette consumption and lower spousal exposure. Women's reports were the strongest predictor of cotinine, with men's self-efficacy providing an independent additive contribution.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"19 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13673860/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149191271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Research note on gravity artifact reduction in IMU signals using Madgwick orientation estimation. 基于Madgwick方向估计的IMU信号重力伪影抑制研究进展。
IF 1.7
BMC Research Notes Pub Date : 2026-10-01 DOI: 10.1186/s13104-026-08032-w
Moritz Toppmöller, Urs-Vito Albrecht
{"title":"Research note on gravity artifact reduction in IMU signals using Madgwick orientation estimation.","authors":"Moritz Toppmöller, Urs-Vito Albrecht","doi":"10.1186/s13104-026-08032-w","DOIUrl":"https://doi.org/10.1186/s13104-026-08032-w","url":null,"abstract":"<p><strong>Objective: </strong>Gravitational artifacts bias Inertial Measurement Unit (IMU) signals, complicating applications such as Seismocardiography (SCG). This study aimed to validate a gravity-artifact reduction algorithm using Madgwick orientation estimation in a controlled, physics-based simulation.</p><p><strong>Results: </strong>A computational phantom was developed using the PyBullet physics engine to generate paired datasets of gravity-free acceleration and simulated IMU signals (with gravitational artifacts). The Madgwick Attitude and Heading Reference System (AHRS) filter was applied to estimate the sensor orientation, followed by a low-pass-filtered gravity projection to subtract artifacts. The gravity-removed acceleration closely matched the gravity-free reference across all axes. Absolute mean differences were consistently below 0.006 m/s<sup>2</sup>, and Pearson correlation coefficients exceeded 0.98. Cross-correlation analysis revealed zero temporal lag, while Bland-Altman analysis showed no proportional bias. The highest precision was observed along the gravitational z-axis. These simulation-only results represent an upper bound under idealized conditions. Experimental validation remains necessary before practical deployment.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"19 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13632254/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149104467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clinical and radiographic evaluation of MTA Flow™ thick as a retrograde filling material in apicoectomy: a preliminary study. MTA Flow™厚层作为逆行填充材料在根尖切除术中的临床和影像学评价:初步研究。
IF 1.7
BMC Research Notes Pub Date : 2026-09-29 DOI: 10.1186/s13104-026-07911-6
Loan-Chi Thi Bui, Son Hoang Le, Hien Thuc Huu Huynh, Bich-Ly Thi Nguyen
{"title":"Clinical and radiographic evaluation of MTA Flow™ thick as a retrograde filling material in apicoectomy: a preliminary study.","authors":"Loan-Chi Thi Bui, Son Hoang Le, Hien Thuc Huu Huynh, Bich-Ly Thi Nguyen","doi":"10.1186/s13104-026-07911-6","DOIUrl":"10.1186/s13104-026-07911-6","url":null,"abstract":"<p><strong>Objective: </strong>To date, limited evidence exists regarding the clinical efficacy of MTA Flow™ Thick as a retrograde filling material in apicoectomy. This study aimed to investigate the clinical and radiographic outcomes over a 6-month follow-up period following apicoectomy with MTA Flow™ Thick as the retrograde filling material.</p><p><strong>Results: </strong>A total of 24 teeth from 16 patients were included in this prospective study. All apicoectomies were performed by a single oral surgeon following a standardised surgical protocol. Clinical and radiographic outcomes were assessed at 3 and 6 months. All clinical symptoms-pain, swelling, and fistulas-showed a statistically significant reduction at both follow-up intervals (p < 0.001 for all). Periapical lesion size decreased at both time points (p < 0.001). Radiographic healing was observed in ≥ 85.5% of cases and demonstrated progressive improvement between the two follow-ups. The overall success rate increased significantly from 83.3% at 3 months to 91.7% at 6 months. Trial registration This study was registered at ClinicalTrials.gov (Identifier: NCT07254143, date 28 November 2025). This is a retrospectively registered trial.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"19 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13621695/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149073557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dynamic changes in total hemoglobin concentration in the frontal region during motion-based video gameplay in patients with Duchenne muscular dystrophy: a pilot study using portable near-infrared spectroscopy (NIRS). 杜氏肌营养不良患者在运动视频游戏过程中额叶区总血红蛋白浓度的动态变化:一项使用便携式近红外光谱(NIRS)的试点研究。
IF 1.7
BMC Research Notes Pub Date : 2026-09-29 DOI: 10.1186/s13104-026-07934-z
Satomi Okano, Sora Mori, Hitomi Nishizawa
{"title":"Dynamic changes in total hemoglobin concentration in the frontal region during motion-based video gameplay in patients with Duchenne muscular dystrophy: a pilot study using portable near-infrared spectroscopy (NIRS).","authors":"Satomi Okano, Sora Mori, Hitomi Nishizawa","doi":"10.1186/s13104-026-07934-z","DOIUrl":"10.1186/s13104-026-07934-z","url":null,"abstract":"<p><strong>Objective: </strong>This exploratory study aimed to investigate the changes in frontal hemoglobin concentration during motion-based video gaming in patients with Duchenne muscular dystrophy (DMD) and to provide preliminary findings regarding the immediate effects of video gaming on cognitive function in this population. We measured changes in total hemoglobin concentration in the left and right frontal regions during rest and gaming using portable near-infrared spectroscopy (NIRS). The Stroop test was administered before and after the intervention as a measure of selective attention, which is a component of cognitive function. Completion time and scores were recorded for the Color Naming (CN) and Incongruent Color Naming (ICN) tasks.</p><p><strong>Results: </strong>Nine patients with DMD participated in a motion-based badminton game. The total hemoglobin concentration changes were significantly higher during rest periods than during gameplay (left: p < 0.001, r = 0.24; right: p < 0.001, r = 0.33). Stroop completion time significantly decreased after the intervention for both CN (p = 0.034, d = 0.78) and ICN (p = 0.028, r = 0.73) tasks. Future studies are warranted to increase the sample size and conduct a randomized controlled trial with a control group. Trial registration University hospital Medical Information Network (UMINCTR, UMIN000060957). Retrospectively registered on March 23, 2026.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"19 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13621501/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149073639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development and technical evaluation of a reusable 3D-printed cytospin funnel for enlarged cytospin generation. 用于扩大细胞自旋产生的可重复使用的3d打印细胞自旋漏斗的开发和技术评估。
IF 1.7
BMC Research Notes Pub Date : 2026-09-29 DOI: 10.1186/s13104-026-08021-z
Christopher Herz, Nicole Weiß, Carlo Mümmler, Michael Gerckens, Nikolaus Kneidinger, Jürgen Behr, Nikolaus A Haas, Marina Nagel
{"title":"Development and technical evaluation of a reusable 3D-printed cytospin funnel for enlarged cytospin generation.","authors":"Christopher Herz, Nicole Weiß, Carlo Mümmler, Michael Gerckens, Nikolaus Kneidinger, Jürgen Behr, Nikolaus A Haas, Marina Nagel","doi":"10.1186/s13104-026-08021-z","DOIUrl":"10.1186/s13104-026-08021-z","url":null,"abstract":"<p><strong>Objective: </strong>Cell fixation on a microscope glass slide is a standard technique for evaluating cellular morphology in a wide range of biosamples. Cytofunnels are used in a cytocentrifuge to direct cells onto the glass slide and generate a \"cytospin\". Commercial cytofunnels have a fixed design with limited cytospin areas, being mostly single-use and are costly. The goal of this study was to design and manufacture a 3D-printed, customized cytofunnel that is cost-efficient, reusable and generates a larger cytospin area. We used an iterative design process using computer-aided design (CAD) software and stereolithography (SLA) printing with biocompatible resin. The funnel prototypes were subjected to a technical evaluation using two independent human pleural effusion specimens, as well as through repeated exposure to two commonly used disinfectants.</p><p><strong>Results: </strong>The 3D-printed cytospin funnel featured larger outlet diameters, increasing the cytospin area approximately threefold with a total manufacturing time of 24 h. The presented reusable funnel design enabled enlarged cytospin areas and increased practical cell-loading capacities under the tested conditions and may support future digital cytology and high-throughput analysis workflows. The published STL file may provide an accessible, sustainable alternative for laboratories, especially in resource-limited settings and may serve as a basis for further customization.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"19 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13621705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149079847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction: Occurrence of hepatitis A virus in Philippine cupped oysters (Magallana bilineata) and green mussels (Perna viridis) collected in the Philippines. 更正:在菲律宾收集的菲律宾杯状牡蛎(Magallana bilineata)和绿贻贝(Perna viridis)中出现甲型肝炎病毒。
IF 1.7
BMC Research Notes Pub Date : 2026-09-24 DOI: 10.1186/s13104-026-08027-7
Abigail S Rustia, Leslie Michelle M Dalmacio, Erniel B Barrios, Patricia Mae M Lagazon, John Carlo G Gosilatar, Dennis D Tanay, Anne Marie M Matubis-Binuya, Christrhea N Udang, Minerva M Manaog, Roel Anthony S Bayangos, Denise Anne B Dela Cruz, Crisanto M Lopez
{"title":"Correction: Occurrence of hepatitis A virus in Philippine cupped oysters (Magallana bilineata) and green mussels (Perna viridis) collected in the Philippines.","authors":"Abigail S Rustia, Leslie Michelle M Dalmacio, Erniel B Barrios, Patricia Mae M Lagazon, John Carlo G Gosilatar, Dennis D Tanay, Anne Marie M Matubis-Binuya, Christrhea N Udang, Minerva M Manaog, Roel Anthony S Bayangos, Denise Anne B Dela Cruz, Crisanto M Lopez","doi":"10.1186/s13104-026-08027-7","DOIUrl":"10.1186/s13104-026-08027-7","url":null,"abstract":"","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"19 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13602556/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Patient treatment engagement scale (PTES) for inflammatory bowel disease: exploring background factors. 炎症性肠病患者治疗参与量表(PTES):探索背景因素
IF 1.7
BMC Research Notes Pub Date : 2026-09-21 DOI: 10.1186/s13104-026-07664-2
Hisao Toyoshima, Chiharu Yashima, Tomohide Akase, Masayoshi Koinuma
{"title":"Patient treatment engagement scale (PTES) for inflammatory bowel disease: exploring background factors.","authors":"Hisao Toyoshima, Chiharu Yashima, Tomohide Akase, Masayoshi Koinuma","doi":"10.1186/s13104-026-07664-2","DOIUrl":"10.1186/s13104-026-07664-2","url":null,"abstract":"<p><strong>Background: </strong>The Patient Treatment Engagement Scale (PTES) is a simplified tool for assessing patient involvement in treatment decision-making, which is an important element of patient-centered care. Shared Decision Making (SDM) is the standard measure in this field; however, it is time-consuming and often difficult for non-physician healthcare professionals to apply in routine practice. Therefore, in this study we adopted PTES as a more pragmatic alternative and exploratively analyzed factors associated with PTES scores among patients with inflammatory bowel disease (IBD).</p><p><strong>Methods: </strong>Individual patient data were collected via a web-based questionnaire. Items potentially associated with PTES, based on previous studies, were screened, and multicollinearity was assessed using Kendall's τ. A multiple regression model was constructed using the ordinary least squares method, and standardized β values and variance inflation factor (VIF) values were calculated. Subgroup analyses were performed separately for ulcerative colitis (UC) and Crohn's disease (CD), and cross-validation was conducted.</p><p><strong>Results: </strong>The main factors associated with PTES scores were the number of treatment options (β = 0.191), presence of complications (β = 0.128), trust in the attending physician (β = 0.119), number of trusted healthcare professionals (β = 0.099), and availability of supportive communities (β = 0.068). The number of treatment options had a particularly strong influence on UC patients, whereas the number of trusted healthcare professionals had a greater impact on CD patients.</p><p><strong>Conclusions: </strong>Providing multiple treatment options and building trust with healthcare providers are crucial factors associated with higher PTES scores, reflecting greater patient engagement in treatment decision-making.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"19 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13595684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A domain-specific annotation framework and expert-annotated corpus for structured analysis of German HNSCC pathology reports. 一个领域特定的注释框架和专家注释语料库,用于结构化分析德国HNSCC病理报告。
IF 1.7
BMC Research Notes Pub Date : 2026-09-21 DOI: 10.1186/s13104-026-08024-w
Sebastian Gubik, Franziska Seger, Andreas Vollmer, Marius Hörner, Tobias Renner, Luca Kohlhepp, Marc Manthey, Urs Müller-Richter, Alexander Kübler, Stephan Hackenberg, Stefan Hartmann
{"title":"A domain-specific annotation framework and expert-annotated corpus for structured analysis of German HNSCC pathology reports.","authors":"Sebastian Gubik, Franziska Seger, Andreas Vollmer, Marius Hörner, Tobias Renner, Luca Kohlhepp, Marc Manthey, Urs Müller-Richter, Alexander Kübler, Stephan Hackenberg, Stefan Hartmann","doi":"10.1186/s13104-026-08024-w","DOIUrl":"10.1186/s13104-026-08024-w","url":null,"abstract":"<p><strong>Objective: </strong>To develop and empirically characterize a domain-specific, block-structured annotation schema and an expert-annotated reference corpus for German-language head and neck squamous cell carcinoma (HNSCC) pathology reports.</p><p><strong>Results description: </strong>We retrieved 631 pathology reports from 145 patients treated at University Hospital Würzburg between January 2021 and March 2024 and split them at the patient level into training, validation, and evaluation subsets. Reports were manually annotated using a hierarchical, span-based schema supporting overlapping and nested annotations. The resulting corpus contains 45 unique labels organized into clinically meaningful blocks and an average of 37-39 annotations per report across subsets. Label frequencies and normalized category distributions were broadly comparable across the three subsets. Fine-grained labels were predominantly applied within their designated blocks, indicating adherence to the intended block structure. The corpus provides a domain-specific reference resource for developing and evaluating structured information-extraction approaches for German HNSCC pathology reports.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"19 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13595725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of yam virus X isolates from Dioscorea trifida in Brazil. 巴西薯蓣病毒X分离株的鉴定。
IF 1.7
BMC Research Notes Pub Date : 2026-09-02 DOI: 10.1186/s13104-026-07987-0
Cintia Paula Feitosa Souza Araújo, Dennis Knierim, Saulo Alves Santos de Oliveira, Rosana Blawid, Paolo Margaria
{"title":"Characterization of yam virus X isolates from Dioscorea trifida in Brazil.","authors":"Cintia Paula Feitosa Souza Araújo, Dennis Knierim, Saulo Alves Santos de Oliveira, Rosana Blawid, Paolo Margaria","doi":"10.1186/s13104-026-07987-0","DOIUrl":"10.1186/s13104-026-07987-0","url":null,"abstract":"<p><strong>Objective: </strong>Yam virus X (YVX; Potexvirus ecsdioscoreae) is a positive-sense, flexuous RNA virus belonging to the family Alphaflexiviridae. It has been first reported from Guadeloupe, a French archipelago located in the Caribbean Sea. In this study, we investigated the virome in yam (Dioscorea spp.) plant material collected in the state of Bahia (Brazil) by high-throughput sequencing (HTS) on Illumina platform. The objective of the investigation was to explore the occurrence of YVX in yam from South America, and to study its genetic diversity compared to the only one YVX genome sequence available in the GenBank public database.</p><p><strong>Results: </strong>An initial investigation by HTS of bulked RNA extracts (n=23, combined into 4 pools) revealed occurrence of YVX only in samples collected in the region of Valença. Subsequent screening by RT-PCR of the individual samples composing the pool uncovered infection with YVX only in Discorea trifida. Total RNA extracts from three infected plants were individually sequenced, resulting in the assembly of three complete genome sequences of YVX, showing ~84% nucleotide identity to the reference sequence from Guadeloupe. Our results contribute to expanding the pool of sequences available for YVX, supporting detection purposes and stimulating additional investigations for future studies on YVX diversity and evolution.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"19 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13540820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148879030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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