{"title":"Timeliness of switching to second-line antiretroviral therapy following virologic failure in Dar es Salaam, Tanzania.","authors":"Majid Hussein Thadeo, George Msema Bwire","doi":"10.1186/s13104-026-07848-w","DOIUrl":"https://doi.org/10.1186/s13104-026-07848-w","url":null,"abstract":"<p><strong>Objective: </strong>Timely transition from first-line to second-line antiretroviral therapy (ART) is critical to prevent treatment failure and minimize resistance. The World Health Organization (WHO) recommends switching within 90 days of confirmed virologic failure (VF). This study assessed the timeliness of switching to second-line ART in three HIV care and treatment clinics in Ilala District, Dar es Salaam.</p><p><strong>Results: </strong>A retrospective cross-sectional study reviewed patient records from 2019 onward. Data were collected using a structured questionnaire in Kobo Toolbox, verified against patient files, and analyzed in SPSS version 29. Of 5456 patients on ART, 213 were on second-line therapy; 121 patients with complete records were included in the analysis. Most participants were female (69.4%), aged 25-34 years (31.4%), married (49.6%), self-employed (38.8%), and living with family (90.9%). Before switching, most patients had at least two viral load tests (94.3%). The most common second-line regimen was Tenofovir + Emtricitabine + Atazanavir/ritonavir (38.8%). Overall, 72.7% of patients were switched within 90 days of confirmed VF, while 27.3% experienced delays. No statistically significant associations were found between timely switching and sociodemographic or clinical factors. Conclusively, more than 25% of patients experienced delayed switching, indicating suboptimal adherence to WHO-recommended timelines in the studied clinics.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147855926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Engineering prosthetic axes for unparallel full arch implants by the virtual selection of abutment orientation and angulation.","authors":"Medhat Sameh Abdelaziz","doi":"10.1186/s13104-026-07808-4","DOIUrl":"https://doi.org/10.1186/s13104-026-07808-4","url":null,"abstract":"<p><p>A technique for virtual determination of the abutments' angulation, orientation, and gingival height for unparalleled osseointegrated dental implants is described. A cone beam computed tomography (CBCT) and an optical scan were performed for the arch to be restored. The wax-up prosthesis was designed using the Exocad software program, and implants were virtually placed over the osseointegrated implants appearing in the CBCT using an implant planning software program. The abutments were virtually placed, and their angulation and orientation were selected according to the virtually designed prosthesis. This technique offers a time- and cost-effective digital solution for engineering unparallel implants' prosthetic axes.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"19 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147855937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Beatrice H Bredt, Mathurin Fatou, Aidi G Lugenge, Dismas S Kamande, Nathalie Liechti, Sarah J Moore, Pie Müller
{"title":"Integrating 3D video tracking with the standard WHO tunnel assay: a proof-of-concept to support improving insecticide-treated nets for mosquito control.","authors":"Beatrice H Bredt, Mathurin Fatou, Aidi G Lugenge, Dismas S Kamande, Nathalie Liechti, Sarah J Moore, Pie Müller","doi":"10.1186/s13104-026-07860-0","DOIUrl":"https://doi.org/10.1186/s13104-026-07860-0","url":null,"abstract":"<p><p>The World Health Organization (WHO) tunnel test is a standardised laboratory assay used to characterise the biological availability and potency of active ingredients on the surface of an insecticide-treated bed net (ITN) against host-seeking mosquitoes. However, the assay provides only endpoint measurements-the proportions of mosquitoes killed and blood-fed - and therefore offers no insight into how mosquitoes interact with the ITN. Therefore, complementary behavioural data would be highly valuable, for example to reveal the extent to which mosquitoes engage with the net, thereby helping to explain differences in endpoint outcomes, or to determine the minimum assay duration required and thus improve throughput. For capturing mosquito behaviour in detail, automated three-dimensional (3D) video tracking offers a powerful approach. Here, we present a proof-of-concept study combining the WHO tunnel assay with Trackit3D, a versatile tracking system, in a laboratory in Tanzania where tunnel assays are routinely performed. The system successfully tracked multiple mosquitoes simultaneously as they were attracted to a rabbit, including measuring the duration of contact with the net, despite typical fluctuations in power supply and lighting conditions. The ability to obtain high-resolution trajectories within the WHO tunnel assay provides new opportunities to enhance the behavioural evaluation of ITNs and strengthens both the interpretability and utility of the assay.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147855995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"EctoShed: a novel gene delivery platform for functional analysis of adipocyte-shed proteoforms.","authors":"Ana Rita Tavanez, Nadia Meincke Egedal, Natasa Stanic, Hande Topel, Jan-Wilhelm Kornfeld","doi":"10.1186/s13104-026-07680-2","DOIUrl":"10.1186/s13104-026-07680-2","url":null,"abstract":"<p><strong>Objective: </strong>The proteolytic cleavage of membrane-bound proteins, ectodomain shedding, functionally expands the reservoir of proteins/peptides available for endocrine crosstalk, and metabolic regulation. However, the functional understanding of secreted proteoforms, including whether they act synergistically or antagonistically with their membrane precursors, is often unknown. We aimed to develop a novel viral vector-based gene delivery platform enabling characterization of both membrane-bound and soluble proteoforms in adipocytes, independent of endogenous shedding. To this end, we elected amine oxidase copper-containing 3 (AOC3) as a target for validation.</p><p><strong>Results: </strong>We describe a novel platform, termed 'EctoShed', achieving adipocyte-specific expression of different proteoforms of AOC3 - full-length and membrane-bound or a soluble AOC3 mimic (m-sAOC3) - by capitalising on the established lentiviral and adeno-associated virus gene delivery systems. In vitro transduction of primary white adipocytes induced significant expression of both isoforms, whilst retaining AOC3 enzymatic activity. In vivo delivery to inguinal white adipose tissue enabled depot-specific AOC3 expression and increased abundance of m-sAOC3 in serum. Mice expressing m-sAOC3 exhibited reduced fat mass and fasting glucose levels. Thus, EctoShed is a novel tool to dissect the functional roles of soluble proteoforms secreted from adipocytes, with broad in vitro and in vivo applications in cardiometabolic health.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"19 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13151326/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147834003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Araz Omar Fatah, Kochr Ali Mahmood, Dawan Jamal Hawezy, Dilshad Hamad Mustafa, Saman Taher Barzinjy, Sirwan Khalid Ahmed
{"title":"Prevalence of biliary gastritis and associated demographic, dietary, and clinical factors among adults in the Kurdistan Region of Iraq: a cross-sectional study.","authors":"Araz Omar Fatah, Kochr Ali Mahmood, Dawan Jamal Hawezy, Dilshad Hamad Mustafa, Saman Taher Barzinjy, Sirwan Khalid Ahmed","doi":"10.1186/s13104-026-07854-y","DOIUrl":"https://doi.org/10.1186/s13104-026-07854-y","url":null,"abstract":"<p><strong>Objective: </strong>Biliary gastritis is an under-recognized inflammatory condition associated with duodenogastric bile reflux and nonspecific gastrointestinal symptoms, often leading to diagnostic challenges. Epidemiological data from the Kurdistan Region of Iraq are limited. This study aimed to estimate the prevalence of biliary gastritis among adults with available diagnostic data and to examine its associations with demographic, lifestyle, dietary, and clinical factors.</p><p><strong>Results: </strong>A descriptive cross-sectional study was conducted between June 2024 and April 2025 among 638 adults recruited from urban and rural healthcare centers. Biliary gastritis was identified based on documented clinical diagnosis and/or prior endoscopic findings. Among participants with available diagnostic documentation (n = 486), the prevalence of biliary gastritis was 26.7%. The mean age of participants was 43.53 ± 15.25 years. Significant associations were observed with sex, marital status, occupation, dietary factors (fast food consumption, fruit and vegetable intake, and caffeine intake), gallstone disease, liver disease, and gastrointestinal symptoms including nausea, vomiting, and abdominal pain (p < 0.05). No significant associations were found with diabetes mellitus, gastroesophageal reflux disease, or physical activity. Multivariable logistic regression identified several demographic, dietary, and clinical variables associated with biliary gastritis. These findings suggest that biliary gastritis represents a notable health concern in this setting, highlighting the importance of dietary modification and improved access to diagnostic services. However, findings should be interpreted cautiously due to the cross-sectional design and reliance on facility-based data. Further longitudinal studies are warranted.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jamie Schnuck Olapo, Sarah A Gunby, Lisa L Strate, David R Flum
{"title":"Dietary modifications to prevent recurrent diverticulitis.","authors":"Jamie Schnuck Olapo, Sarah A Gunby, Lisa L Strate, David R Flum","doi":"10.1186/s13104-026-07779-6","DOIUrl":"https://doi.org/10.1186/s13104-026-07779-6","url":null,"abstract":"<p><p>Each year, millions of people experience an episode of diverticulitis, accompanied by abdominal pain, gastrointestinal symptoms, and expensive healthcare utilization. Rates of recurrence following recovery from an episode are high, ranging from 20 to 50% depending on how this is measured. A number of dietary and lifestyle factors have been found to be associated with an increased risk of developing incident diverticulitis; while these have been suggested to play a role in preventing recurrence, there is limited evidence to support this claim. Clinicians often recommend a high fiber diet after recovery from an episode of diverticulitis, however, numerous other dietary patterns have been proposed to provide benefit. Anecdotal evidence suggests that there are widespread beliefs among patients with diverticulitis about dietary triggers and modifications that are effective at reducing disease recurrence and symptom burden. We aimed to gain a better understanding of what current dietary modifications are being attempted by patients with a history of diverticulitis episode and surveyed 430 people. We found that a high fiber diet was the most common dietary change, made in 59% of survey respondents, followed by the Mediterranean diet by 26%. Among patients who adopted a Mediterranean diet, adherence was modest in 45% and strict/high in 20%. These findings suggest patients commonly make dietary changes motivated by a history of diverticulitis, and that further research in dietary interventions for this condition may be embraced by patients.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Simultaneous consumption of vegetable salad with bread attenuates postprandial serum glucose elevation in healthy adults: a single-ingestion open-label crossover trial.","authors":"Mengwei Yuan, Naoki Kawada, Yumi Takeda, Ryosuke Matsuoka, Kazunori Utsunomiya","doi":"10.1186/s13104-026-07836-0","DOIUrl":"https://doi.org/10.1186/s13104-026-07836-0","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to evaluate the effect of simultaneous consumption of vegetables and bread on postprandial serum glucose concentration. In total, 15 healthy men participated in this single-ingestion, open-label, non-randomized crossover trial. Participants were given meals (bread vs. bread with vegetable salad) after a night of fasting in a non-randomized sequence. At 0, 15, 30, 45, 60, 90, and 120 min following the consumption of the test meal, blood samples were collected to determine the serum levels of glucose, insulin, glucose-dependent insulinotropic polypeptide, and triglycerides.</p><p><strong>Results: </strong>Results revealed that serum glucose and insulin levels were significantly lower after 45 and 60 min in participants who consumed bread with vegetable salad than in those who only consumed bread. This emphasizes the potential benefit of simultaneously consuming vegetables and bread as an effective dietary strategy for preventing postprandial blood glucose elevation.</p><p><strong>Trial registration: </strong>UMIN Clinical Trials Registry (UMIN-CTR), UMIN000053931, registered on March 22, 2024.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The persistent threat of malaria in Bishoftu Town: twelve-year trends and their implications for Addis Ababa.","authors":"Buze Chala Dufera, Hassen Mamo","doi":"10.1186/s13104-026-07853-z","DOIUrl":"https://doi.org/10.1186/s13104-026-07853-z","url":null,"abstract":"<p><p>Bishoftu town, located about 40 km from the largely malaria-free Addis Ababa, remains malaria-endemic. Factors that possibly heighten exposure risk include abundant water bodies, increased visitors during events such as the Irreechaa festival, climate change, and changing land-use around the town. Frequent travel and strong interconnections raise concerns about spillover to Addis Ababa via infected people or mosquitoes. The potential spread of the invasive urban vector Anopheles stephensi is an additional concern, although there is currently little evidence of its introduction in Bishoftu. Future surges in transmission could be facilitated by infrastructure projects most particularly the construction of a major new airport in Bishoftu that will increase visitor traffic. Analysis of 252,610 blood films from the Bishoftu Town Health Office (January 2011 - December 2022) revealed 5,696(2.3%) malaria cases, with Plasmodium vivax at 63.8% and P. falciparum at 31.0%. The highest incidence (804 cases) occurred in 2017, with individuals > 14 constituting 66.3% of cases. Findings indicate persistent malaria with a fluctuating trend, highlighting the need for aggressive surveillance and targeted control measures to combat malaria in Bishoftu and prevent its spread to Addis, considering demographic shifts, increased mobility, and the development of recreational water pools associated with development projects.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147834009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hendrik Bertram, Muhammad Jawad, Susann Michanski, Inga Tiemann, Armin O Schmitt, Mehmet Gültas
{"title":"Whole-genome resequencing data of the Ixworth chicken breed.","authors":"Hendrik Bertram, Muhammad Jawad, Susann Michanski, Inga Tiemann, Armin O Schmitt, Mehmet Gültas","doi":"10.1186/s13104-026-07837-z","DOIUrl":"https://doi.org/10.1186/s13104-026-07837-z","url":null,"abstract":"<p><strong>Objectives: </strong>The Ixworth chicken is a British dual-purpose breed and mostly maintained by small-scale farmers. Due to legislation regarding the ban on male chick culling in European countries, such as in Germany, renewed interest has arisen in rearing dual-purpose chickens that provide both meat and eggs from the same genetic line. This dataset was generated within the scope of projects aiming to evaluate the viability of dual-purpose breeds for sustainable and welfare-oriented poultry production. One of the objectives was to characterize the genetic potential of the Ixworth chicken as a model for breeding programs that combine conservation and practical use in ecological farming systems.</p><p><strong>Data description: </strong>Liver samples from 49 male Ixworth chickens were collected after scheduled slaughter at the Campus Frankenforst of the Faculty of Agricultural, Nutritional and Engineering Sciences of the University of Bonn, Germany. Genomic DNA was extracted and subjected to whole-genome resequencing using the Illumina NovaSeq 6000 platform. The dataset provides high-resolution genomic information on a rare breed with a pure dual-purpose background. This resource represents the first public sequencing dataset of the Ixworth chicken and thus offers a valuable foundation for future studies on genetic diversity, conservation genomics, and breeding strategies for sustainable poultry production.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"19 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13137572/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of inpatient antibiotic prescribing patterns using WHO indicators in Northern Ethiopia: a prospective cross-sectional study identifying stewardship gaps.","authors":"Haftom Yirga Tsegay, Berhane Yohannes Hailu, Gebrehiwot Gebremedhin Tafere, Filmon Beyenne Demoz, Werkey Araya Tekleargis, Kald Beshir Tuem","doi":"10.1186/s13104-026-07852-0","DOIUrl":"https://doi.org/10.1186/s13104-026-07852-0","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to evaluate inpatient antibiotic prescribing patterns and identify antimicrobial stewardship (AMS) gaps using World Health Organization (WHO) antibiotic use indicators at Ayder Comprehensive Specialized Hospital (ACSH), a major tertiary hospital in Northern Ethiopia.</p><p><strong>Results: </strong>Among 1,684 admitted patients, 865 (51.4%) received at least one antibiotic. A total of 1,491 antibiotics were prescribed, with a mean of 1.7 ± 0.7 antibiotics per patient and an average treatment duration of 5.9 ± 3.9 days. Nearly all antibiotics were prescribed by generic name (98.9%) and from the national Essential Medicines List (100%). Injectable formulations accounted for 90.9% of prescriptions. Ceftriaxone (41.0%), metronidazole (21.9%), and vancomycin (9.4%) were the most commonly used antibiotics. Watch-class antibiotics constituted 64.1% of prescriptions. More than half of antibiotic use was for therapeutic purposes (54.3%), of which 95.5% was empirical. Culture testing was performed in only 2.4% of patients, and antimicrobial susceptibility testing in 0.3%. Stock-outs affected 24.3% of antibiotics, with a mean duration of 4.8 days per month. Longer hospital stays, comorbidities, and severe clinical conditions, including sepsis and pneumonia, were associated with increased antibiotic use.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}