BMC Research Notes最新文献

{"title":"A pilot study using eDNA collected from soil and active air samplers to detect terrestrial vertebrates in an open grassland habitat of central Queensland, Australia.","authors":"Emma L Gray, Faith M Walker, Daniel E Sanchez, Savannah J Marriott, Andrew M Baker","doi":"10.1186/s13104-025-07302-3","DOIUrl":"10.1186/s13104-025-07302-3","url":null,"abstract":"<p><strong>Objective: </strong>Small mammals such as the Julia Creek dunnart (Sminthopsis douglasi) may be difficult to detect using traditional trapping methods. Here, we conducted a pilot study to determine whether eDNA collected from soil and/or air could detect the presence of terrestrial vertebrates, including S. douglasi, in a semi-arid, open grassland environment.</p><p><strong>Results: </strong>Airborne eDNA analysis returned vertebrate DNA from five sample sites (n = 7), whereas soil eDNA analysis returned vertebrate DNA from a single site (n = 7). The Julia Creek dunnart was not detected in any of the experimental samples. However, several airborne eDNA samples did return strong matches to three terrestrial vertebrates, the long-haired rat (Rattus villosissimus), red kangaroo (Osphranter rufus) and brown quail (Synoicus ypsilophorus), all native species known to occur commonly in the study area. Overall, our preliminary findings suggest that the effectiveness of airborne and soil-derived eDNA in detecting terrestrial vertebrates was constrained by high human signal and low sampling intensity. For future studies, we recommend a number of field and lab-based refinements to increase the likelihood of detecting more taxa, particularly those that occur at low density.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"237"},"PeriodicalIF":1.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12117675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144156587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The LowSugarBerry trait phenotyped at single fruit and population levels.","authors":"Antoine Bigard, Luciana Wilhelm de Almeida, Dargie T Berhe, Die Wu, Hernán Ojeda, Anne Pellegrino, Charles Romieu, Laurent Torregrosa","doi":"10.1186/s13104-025-07289-x","DOIUrl":"10.1186/s13104-025-07289-x","url":null,"abstract":"<p><strong>Objective: </strong>The grapevine is one of the most widely grown perennial crops worldwide. As for other species displaying clusters of small fruits, the development of single berries within a bunch is asynchronous and heterogeneous. Because of this, the study of water and solute accumulation kinetics and balance at the organ level cannot be directly extrapolated from populations of fruits. In order to have an original and reliable data sets on the accumulation of the main solutes and water in the grapevine fruits, from the onset of sugar importation to the end of phloem unloading, we analyzed the composition of populations of berries sorted according to their density and also single berry samples.</p><p><strong>Data description: </strong>Contents in primary and secondary metabolites and cations and water during fruit ripening in a panel of varieties displaying a range of metabolic features, including genotypes able to import water at low fruit osmotic potential. These original data sets are suitable to revisit some aspects of the accumulation of main solutes and water in the grapevine fruit and/or to perform comparative studies with other fleshy fruits.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"236"},"PeriodicalIF":1.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12117671/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144156525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of a thermochemical antibody stripping method for enhanced tyramide signal amplification-based opal multiplex immunohistochemistry in fragile tissues.","authors":"Xiaotong Yu, Yan Song, Li Chen, Lixiang Xue, Jianling Yang, Chen Huang","doi":"10.1186/s13104-025-07301-4","DOIUrl":"10.1186/s13104-025-07301-4","url":null,"abstract":"<p><strong>Objective: </strong>Tyramide signal amplification (TSA)-induced Opal multiplex immunohistochemistry (mIHC) represents an advanced methodology for the in-situ detection of multiple target proteins. A critical aspect of this technique is the complete removal of primary and secondary antibodies to prevent signal cross-reaction. However, current antibody stripping methods exhibit several limitations, particularly when applied to tissues prone to delamination. Microwave treatment may compromise tissue integrity, additionally, the effectiveness of chemical reagents can be sensitive to variations in temperature, pH, and concentration. This study aims to optimize antibody stripping strategies specifically for use in Opal mIHC protocols, addressing limitations of current methods which particularly for tissues prone to delamination.</p><p><strong>Results: </strong>Evaluation of microwave oven-assisted antibody removal (MO-AR), chemical reagent-based antibody removal (CR-AR), and hybridization oven-based stripping at 50 °C (HO-AR-50) and 98 °C (HO-AR-98) revealed that MO-AR and HO-AR-98 most effectively removed primary and secondary antibodies. In five-color mIHC on mouse kidney sections, both methods yielded strong target-specific signals. However, in brain tissue sections prone to delamination, HO-AR-98 better preserved tissue integrity compared to MO-AR and supported effective multi-target staining. These findings establish a novel thermochemical stripping method compatible with TSA-based Opal mIHC, enhancing its utility in research and clinical diagnostics.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"235"},"PeriodicalIF":1.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12117710/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144156590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of geopolymer membranes using clay and some industrial wastes to remove the organic load of pharmaceutical wastewater.","authors":"Abdulrazzaq Hammal","doi":"10.1186/s13104-025-07299-9","DOIUrl":"10.1186/s13104-025-07299-9","url":null,"abstract":"<p><p>The importance of geopolymeric materials has increased in recent years due to their high effectiveness in water treatment by multiple techniques such as adsorption and filtration. Therefore, research has increased related to the preparation of geopolymers from different raw materials. In this research geopolymeric membranes were prepared from Syrian clay by calcinted it at 600˚C for 120 min, then it was mixed with an activated alkaline solution (NaOH + Na₂SiO₃: SiO₂/Na₂O = 1.3). The effect of adding quantities of chamotte waste, ceramics waste, and glass waste on the properties of the geopolymeric membrane was studied. The porosity decreased significantly due to waste additions, the decrease in porosity led to a decrease in water flow, while the compressive strength of the membranes increased with the effect of waste addition. The prepared membranes were used to remove organic pollutants from pharmaceutical wastewater in two stages, the first stage was absorbed by the prepared membrane powder, and the second stage was filtration by the prepared membrane. The tests showed good efficiency in removing organic pollutants, and the results of the bacteriological tests indicated good effectiveness of the prepared membranes in removing bacterial contamination from the treated water sample.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"232"},"PeriodicalIF":1.6,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12105329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144149320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reference intervals of haematological indices for young adults with sickle cell anaemia in Southwestern Nigeria.","authors":"Muritala A Asafa, Ibrahim O Ahmed, Mary B Agim, Taofik A Fabinu, Simeon O Kusoro, Blessing T Ogunoye, Oluwadare Ogunlade, Rahman A Bolarinwa","doi":"10.1186/s13104-025-07303-2","DOIUrl":"10.1186/s13104-025-07303-2","url":null,"abstract":"<p><strong>Objective: </strong>Reference intervals (RIs) are crucial for the interpretation of results and overall patient management. There are currently no known established reference values for haematological parameters for patients with sickle cell anaemia (SCA) in Nigeria, making it difficult for clinicians to interpret and diagnose abnormalities appropriately. This study aimed at establishing RIs of some haematological indices for individuals with SCA.</p><p><strong>Results: </strong>Generally, the reference interval for Haematocrit (%) is 17.8 to 29.7, while the WBC (x 10⁹/l) ranged between 5.9 and 12.1 and the platelet count (x 10⁹/l) is 166-465. For the male participants, the reference interval for haematocrit (%) was found to be between 17.8 and 29, the WBC (x 10⁹/l) ranged between 6.7 and 12.1 and the platelet count (x 10⁹/l) was between 166 and 399. The female subjects had a haematocrit (%) of between 18.7 and 28.5, WBC of between 5.9 10⁹/l and 13.0 × 10⁹/l and the platelet count (x 10⁹/l) was between 225 and 465.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"233"},"PeriodicalIF":1.6,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12107978/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144149358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of non-ionizing radiation on the thyroid gland in rats.","authors":"Zeynab Seyedi Sarhad, Amirali Ebrahimbabaei, Alireza Tavassoli, Manzarbanoo Shojaeifard","doi":"10.1186/s13104-025-07297-x","DOIUrl":"10.1186/s13104-025-07297-x","url":null,"abstract":"<p><strong>Objectives: </strong>This study evaluated the effects of non-ionizing electromagnetic radiation on rat thyroid function and histopathology. Forty female and thirty male Sprague-Dawley rats (200-220 g, 2 months old) were exposed to 2.45 GHz Wi-Fi, mobile jammer radiation, or a sham condition. In Group A, male rats were exposed to Wi-Fi or mobile jammers for 2 h daily for two weeks. The devices were located within a one-meter radius of the animal cage, either on or off. In the Sham group, the experimental setup was like the other groups, but the irradiating devices were turned off. Group B included non-pregnant females, pregnant (exposed and control subgroups), and their offspring. Thyroid hormones in the serum were measured, and the histology was microscopically analyzed, focusing on areas of colloid and epithelium in the thyroid follicles.</p><p><strong>Results: </strong>Compared to the control group, T4 hormone levels were significantly different in male rats exposed to mobile jammer radiation (p-value = 0.037). In group B, significant differences were found solely in the male offspring regarding T3 levels due to jammer exposure (average = 109.00 for male offspring in the experiment vs. average = 65.50 for those in the control, p-value < 0.001). Additionally, histopathological findings indicated significant differences as well. These results highlight a potential link between exposure to electromagnetic radiation and changes in thyroid endocrine and histological parameters. Our findings suggest that ongoing assessment of existing safety guidelines on non-ionizing radiation exposure is necessary, especially concerning its effects on thyroid hormone levels and follicular histology.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"231"},"PeriodicalIF":1.6,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12103772/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing dental students' awareness of anaphylactic shock caused by local anesthetic injection: a cross-sectional, descriptive-analytical study.","authors":"Fahimeh Pakravan, Bahare Jabbari, Mehdi Nasr Isfahani, Adel Tabesh, Latife Jabbari","doi":"10.1186/s13104-025-07296-y","DOIUrl":"10.1186/s13104-025-07296-y","url":null,"abstract":"<p><strong>Objective: </strong>Anaphylaxis, a life-threatening allergic reaction with rapid onset and multi-organ involvement, poses a serious risk in dental emergencies, particularly after local anesthetic injections. Other potential triggers include antibiotics, chlorhexidine, iodoform, and latex. Prompt diagnosis and management are crucial for healthcare professionals, yet some dental students lack the necessary knowledge to handle such emergencies effectively. This study aims to assess the awareness of final-year dental students at Isfahan University of Medical Sciences regarding anaphylactic shock induced by local anesthetic injections.</p><p><strong>Results: </strong>A total of 206 students (115 females, 91 males), aged 23-27 years, participated. The most common age was 24 (22.8%). Average knowledge scores were 4.04 for anaphylaxis reactions, 2.17 for epinephrine prescription, and 1.50 for symptoms and signs. Female students scored slightly higher on anaphylaxis awareness than males (4.11 vs. 3.95, P = 0.347). Students aged 23 and 27 demonstrated greater knowledge (4.45 and 4.24, P = 0.068). No significant differences were found based on gender, age, or year of entry. Few students had encountered or treated anaphylaxis cases, highlighting the need for improved education on epinephrine use and symptom recognition.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"230"},"PeriodicalIF":1.6,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12103749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of effectiveness of health education bundle to overcome vaccine hesitancy in mothers: single blinded randomized study.","authors":"Mounika Bazar, Kiran N Baliga, Anupama Nayak Panakaje, S R Ravikiran","doi":"10.1186/s13104-025-07291-3","DOIUrl":"10.1186/s13104-025-07291-3","url":null,"abstract":"<p><strong>Objective: </strong>Vaccine Hesitancy (VH) challenges previously perceived attitudes of a simple dichotomy of \"accept\" or \"reject\". This is not just due to people being uninformed or misinformed but rather due to multiple forms of distrust. It is criticized as a vague notion with an uncertain hypothetical background. Therefore, this study aimed to assess the baseline knowledge and immunization practices of postnatal mothers and their hesitancy after intervention with the health education tool 'SuBaDRa' and compare it with those of the control group.</p><p><strong>Result: </strong>This hospital-based, single-blinded randomized control study was performed for 2 years with 272 (136 per group) postnatal mothers (booked cases with immunization cards and access to a smartphone) in Karnataka, India. 'SuBaDRa', a tailored health education tool, was used to counsel the intervention group: Presenting Sustainable initiatives by the government; assessing mothers' Baseline immunization knowledge and postintervention revaluations via the 'WHO SAGE questionnaire', Dissipating knowledge via health education and Reinforcement with social media applications. The control group was counseled about essential newborn care. The vaccine delay of infants at birth and at 6, 10, and 14 weeks and 9 months of age was assessed. The secondary outcome measures mothers' attitudes toward immunization postintervention. The vaccine was considered delayed if it was received later than 2 weeks after the recommended age. The characteristics of the study subjects, i.e., postnatal mothers with eligible newborns, were analyzed via descriptive statistics. These characteristics were compared between the intervention and control groups via the Chi-square (χ²) test and Fischer's exact test. The results revealed that the intervention group vaccine delays at 6, 10, 14 weeks and 9 months were 5.9%, 3%, 0.7% and 11.9%, respectively, and the control group vaccine delays were 20%, 28.9%, 55.6% and 48.1%, respectively, with p values of 0.001 at 6 weeks and 0 for the rest, all of which were highly significant. Trial registration The study was registered on Clinical Trials Registry - India (CTRI) with the registration number (CTRI/2021/08/035749), registered on (18/08/2021).</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"229"},"PeriodicalIF":1.6,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12102990/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144131765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing resazurin-based viability assays for P-MSC/TER308 cell line to enhance results reliability.","authors":"Jessica Petiti, Laura Revel, Carla Divieto","doi":"10.1186/s13104-025-07298-w","DOIUrl":"10.1186/s13104-025-07298-w","url":null,"abstract":"<p><strong>Objective: </strong>The results of this research contribute to the LifeSaver project, which focuses on reducing neonatal and infant mortality resulting from preterm births. The project aims to create an in vitro system simulating prenatal conditions to screen and analyze chemicals and pharmaceuticals, establishing scientifically justified regulations for their use during pregnancy. Because several papers have recently identified data inconsistencies in pre-clinical studies, a key part of the project involves optimizing cellular cytotoxicity assays to enhance the reliability of pharmacological and toxicity screening for drugs and environmental contaminants.</p><p><strong>Results: </strong>The resazurin-based viability assay was chosen as the primary method due to its widespread adoption and simplicity in assessing drug cytotoxicity. This work describes the optimization of the resazurin-based viability assay on the P-MSC/TERT308 cell line, a placenta-derived mesenchymal stem cell used within the LifeSaver project. By applying our previously described and validated Standard Operating Procedure, we fine-tuned experimental parameters, consistently obtaining reliable results with measurement uncertainty of less than 10%.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"228"},"PeriodicalIF":1.6,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12100786/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sexual harassment complaints across BSE100 companies: a longitudinal dataset (2013-2023).","authors":"Divya R, Gopi Ranga Nath V","doi":"10.1186/s13104-025-07294-0","DOIUrl":"10.1186/s13104-025-07294-0","url":null,"abstract":"<p><strong>Objective: </strong>This dataset offers empirical support for understanding the number of sexual harassment complaints across various industries and sectors, and for analysing patterns over a decade. The data was manually collected from the annual integrated report or business responsibility report of each company on the BSE100 list. This dataset can be utilised by the Indian Ministry of Women & Child Development and related stakeholders as a foundation for studying the impact of previous interventions and policy changes on complaint reporting rates. Additionally, it can also be used to analyse patterns and trends and give way to revisions to existing legal frameworks and improvements to grievance redressal mechanisms for sexual harassment complaints.</p><p><strong>Data description: </strong>Data includes descriptive statistics for all variables and compares complaints received between each year and the subsequent year, with a total of 10 year-to-year comparisons for the period: 2013-2024. The variables are: (1) List of BSE100 Companies, (2) Industry, (3) Sector, (4) Number of sexual harassment complaints received for the periods: 2013-2024, (5) Number of sexual harassment complaints pending during the periods: 2013-2024.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"227"},"PeriodicalIF":1.6,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101009/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}