BMC Research Notes最新文献

{"title":"Pilot application of an inflammation and physiological dysregulation index based on noninvasive salivary biomarkers.","authors":"Andrey I Egorov, William Xue, Jason Kobylanski, Miyu Fuzawa, Shannon M Griffin, Timothy J Wade, Michael Nye","doi":"10.1186/s13104-024-07056-4","DOIUrl":"10.1186/s13104-024-07056-4","url":null,"abstract":"<p><strong>Background and objective: </strong>Noninvasive salivary biomarkers can be used to assess the cumulative subclinical health impacts of social and environmental stressors. We evaluated seven salivary biomarkers of neuroendocrine and immune functions and a group index of physiological dysregulation based on these biomarkers in a pilot survey in a socioeconomically disadvantaged population.</p><p><strong>Results: </strong>Participants recruited at subsidized public housing projects in Denver, Colorado, completed a sociodemographic, behavioral, and health questionnaire and used passive drool samplers to collect five saliva samples over the course of 1 day. Samples were tested for the neuroendocrine biomarkers alpha-amylase, cortisol, and dehydroepiandrosterone (DHEA) as well as immune system/inflammation biomarkers C-reactive protein (CRP), interleukin (IL)-1β, IL-6 and total immunoglobulin A (IgA). A composite Inflammation and Physiological Dysregulation Index (IPDI) was calculated as a count of potentially unhealthy values of dichotomized biomarkers. In 20 individuals who completed the survey (average age 46 years, 75% females), allergy to house dust was significantly associated with increased IPDI (adjusted for age) and significantly increased odds of potentially unhealthy values of total IgA, IL-1β, and CRP. Age, obesity, diabetes, allergy to pollen, recent loss of employment, and depression, anxiety, and stress scores from the standard DASS-21 questionnaire were positively but not significantly (0.05 < p < 0.2) associated with IPDI. This project demonstrated an application of a composite index based on noninvasive salivary biomarkers to assess subclinical health impacts of chronic social stressors.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"53"},"PeriodicalIF":1.6,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11796071/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143254838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OsHMA3 overexpression works more efficiently in generating low-Cd rice grain than OsNramp5 knockout mutation.","authors":"Yuejing Gui, Joanne Teo, Dongsheng Tian, Raji Mohan, Zhongchao Yin","doi":"10.1186/s13104-025-07112-7","DOIUrl":"10.1186/s13104-025-07112-7","url":null,"abstract":"<p><strong>Objective: </strong>Cadmium (Cd) is a highly toxic metal element and a carcinogen to humans. Rice is prone to taking up Cd from paddy fields and accumulating it in grain, which raises health concerns for rice consumers. OsNramp5 is a major transporter for Cd and manganese (Mn) uptake in rice, whereas OsHMA3 is a tonoplast-localized transporter involved in Cd detoxification in vacuoles. In this study, we compared the efficiency of OsNramp5 knockout mutation and OsHMA3 overexpression in reducing Cd content in rice grain.</p><p><strong>Results: </strong>The grain Cd content of the OsNramp5 knockout mutants was significantly lower than that of the wild-type rice T5105. However, these mutants still had much higher grain Cd content than the previously reported OsNramp5 mutants or the OsHMA3 overexpression lines developed in our previous study. Pyramiding the OsNramp5 mutant allele and the OsHMA3 transgene in a single line did not result in an additional reduction in grain Cd content. The OsNramp5 gene in T5105 has a haplotype II promoter, and its knockout mutation also partially reduced Mn content in rice grain. Our results demonstrate that OsHMA3 overexpression works more efficiently in generating low-Cd rice grain than OsNramp5 knockout mutation without affecting Mn uptake in rice.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"55"},"PeriodicalIF":1.6,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11800450/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143254837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tympanoplasty in Northern Ethiopia: success rates, failure factors, and audiometric improvements.","authors":"Filmawit Gebremeskel Asfaha, Bethelhem Birhanu Tesfa, Lemlem Weldegerima Gebremariam, Andom Geremew, Seid Temam, Girmatsion Fisseha Abreha","doi":"10.1186/s13104-025-07123-4","DOIUrl":"10.1186/s13104-025-07123-4","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to determine the success rate of tympanoplasty, the factors associated with failure rate, and audiometric gains after the surgical procedure in Ethiopia.</p><p><strong>Results: </strong>There were 82 successful grafts take out of the 92 tympanoplasty procedures with an overall success rate of 89.1%. Females, who had tympanoplasty had 45 (91.8%) successful grafts while 37 (86.0%) successful grafts for male. Of the total tympanoplasties, 50(53.4%) of it were done in the left ear. Ear discharge was found to be a significant predictor of tympanoplasty failure rate [AOR = 9.6, 95% CI (1.1 - 88.8)]. There was a significant gain of air conduction with a p-value of < 0.001 after the tympanoplasty procedure which was about 13 dB mean difference.</p><p><strong>Conclusions: </strong>In this study, only post-operative ear infection was found to decrease the success rate of tympanoplasty significantly. It also revealed that tympanoplasty improves hearing ability. We recommend a strict application of infection prevention and control strategies, and adequate and comprehensive follow-up of patients including audiometric assessment.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"52"},"PeriodicalIF":1.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11792363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143122146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Severe anaemia in people with HIV: demographic, clinical and renal correlates.","authors":"Kingsley Kamvuma, Sody Munsaka, Sepiso K Masenga, John Amos Mulemena, Christopher Newton Phiri, Michelo Miyoba, Benson M Hamooya","doi":"10.1186/s13104-025-07100-x","DOIUrl":"10.1186/s13104-025-07100-x","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the sociodemographic, clinical and renal correlates associated with severe anaemia among people with HIV.</p><p><strong>Methods: </strong>We conducted a cross-sectional analysis of people with HIV on antiretroviral therapy (ART) for at least 6 months, stratified by anaemia status. Anaemia was defined based on the World Health Organisation (WHO) classification, as haemoglobin concentration lower than normal i.e. <12 g/dl in females and < 13 g/dl in males and the primary outcome, severe anaemia, as a haemoglobin level below 8 g/dl according to the World Health Organisation.</p><p><strong>Results: </strong>The study comprised 372 participants receiving ART, of whom 236 (63.4%) were females. The mean age ± SD of the participants was 44.8 ± 12.4 years. The overall prevalence of severe anaemia was 7.8% (95% CI: 0.053-0.111). In multivariable logistic regression analysis, factors significantly associated with severe anaemia were female sex (Adjusted Odds Ratio (AOR: 14.3, 95% CI: 2.14-126.6), albumin (AOR: 0.93 95% CI: 0.88-0.98) and creatinine levels (AOR: 1.01 95% CI: 1.00-1.03).</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"51"},"PeriodicalIF":1.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11792207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143122042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Open challenges for the automatic synthesis of clinical trials.","authors":"Olivia Sanchez-Graillet, David M Schmidt, Christian Kullik, Philipp Cimiano","doi":"10.1186/s13104-025-07121-6","DOIUrl":"10.1186/s13104-025-07121-6","url":null,"abstract":"<p><strong>Objective: </strong>An important criterion for selecting clinical trials to be compared in systematic reviews and meta-analyses is that they measure the same outcomes. However, this represents a challenge as there is a wide variety of outcomes, and it is difficult to standardize them for comparing clinical trials containing them. To address this challenge, we utilized our annotated dataset, which includes 211 abstracts of clinical trials related to glaucoma and type 2 diabetes mellitus. We then developed a tool that provides an overview of the annotated clinical trial information and enables users to group them by outcomes.</p><p><strong>Results: </strong>Using our visualization tool, we formed groups of outcomes and their respective clinical trials. We were able to determine the most common outcomes in clinical trials for these diseases. As a case study on diabetes, we compared our outcomes with those consented by diabetes stakeholders and found that many of the grouped outcomes are aligned with the consented ones. This demonstrates that tools such as the one presented can help standardize clinical outcomes, which in turn help in the synthesis of clinical trials. Finally, we also offer some recommendations that could help in the automation of clinical trials based on outcome standardization.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"50"},"PeriodicalIF":1.6,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11789334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The association between consumption of ultra-processed foods and sperm quality parameters: a cross-sectional study.","authors":"Mitra Soltani, Mohammad Reza Ahmadi, Zainab Shateri, Zahra Maghsoudi, Milad Rajabzadeh-Dehkordi, Moein Askarpour, Amir Hossein Asadi, Mehran Nouri","doi":"10.1186/s13104-025-07107-4","DOIUrl":"10.1186/s13104-025-07107-4","url":null,"abstract":"<p><strong>Background: </strong>While recent studies suggest a correlation between unhealthy dietary patterns, oxidative stress, inflammation, and male infertility, the potential association between ultra-processed foods (UPFs) and male infertility remains underexplored. Therefore, the aim of the present study was to investigate the association between UPF intake and male infertility by evaluating sperm quality parameters.</p><p><strong>Methods: </strong>The participants (n = 260) of the current cross-sectional study were recruited from an infertility center in Isfahan Province, Iran. Four semen parameters-such as total sperm motility, sperm concentration, sperm volume, and normal sperm morphology-were evaluated. Also, the participants' food intake was assessed using a validated 168-item food frequency questionnaire. Moreover, the NOVA system was employed to calculate the UPF index. The association between UPFs and sperm parameters was analyzed using logistic regression.</p><p><strong>Results: </strong>In the crude model, no significant associations were observed between the second and last tertiles of UPFs with abnormalities in sperm concentration, total motility, and morphology (p > 0.05 for all). However, after adjusting for age, marriage duration, body mass index, physical activity, depression, anxiety, stress, energy intake, cigarette history, and mineral and vitamin supplements, a significantly higher association was identified between the second tertile of UPFs and abnormalities in sperm concentration (odds ratio (OR) = 3.962, 95% confidence interval (CI): 1.345-11.670, p = 0.013).</p><p><strong>Conclusions: </strong>In conclusion, although the analysis did not find significant associations between UPF consumption and impaired sperm motility and morphology, it revealed significant trends linking higher UPF intake with lower sperm concentration. If future studies confirm these results, they could aid in designing interventional and preventive programs aimed at addressing infertility in men of reproductive age in the field of public health.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"48"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11786443/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Profile of outer membrane proteins of carbapenem-resistant Gram-negative bacilli in Ghana.","authors":"Francis S Codjoe, Fleischer C N Kotey, Eric S Donkor","doi":"10.1186/s13104-024-07070-6","DOIUrl":"10.1186/s13104-024-07070-6","url":null,"abstract":"<p><strong>Objective: </strong>Carbapenem resistance is a major global health threat, but insights on its molecular determinants are scanty in sub-Saharan Africa, the predominant global antimicrobial resistance hotspot. This study aimed to profile outer membrane proteins (OMPs) of 111 carbapenem-resistant Gram-negative bacteria recovered from a broad spectrum of clinical specimens from Ghana.</p><p><strong>Results: </strong>The OMPs of Pseudomonas aeruginosa produced decreased amounts of OprD or the porin was completely lost, except in 5.9% (n = 3) of the isolates which had high-level porins. For Acinetobacter baumannii, 96.8% (n = 30) expressed loss of OprF. One carbapenemase non-PCR-positive isolate with high-level porin expression was observed. In A. baumannii, the major and significant band on SDS-PAGE was ~ 35 kDa. There were substantial numbers of unrelated porin expression among the isolates. Particularly, OmpC/F or OmpK35/37 expression was deficient. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis for Escherichia coli, Klebsiella aerogenes, Klebsiella pneumoniae, and other Enterobacterales had OmpC and OmpF absent or markedly reduced compared to the control strains. Overall, porin loss was a major mechanism underlying carbapenem resistance among the isolates, suggesting that in carbapenem-resistant organisms that seem to lack known carbapenem resistance genes, porin loss may be the underlying carbapenem resistance mechanism.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"49"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11786416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feasibility of enzalutamide on patients with recurrent non-muscle-invasive bladder cancer with marker tumors: phase I study.","authors":"Takashi Kawahara, Shuya Kandori, Takahiro Kojima, Bryan J Mathis, Masanobu Shiga, Hiroyuki Nishiyama","doi":"10.1186/s13104-025-07128-z","DOIUrl":"10.1186/s13104-025-07128-z","url":null,"abstract":"<p><strong>Objective: </strong>Recent preclinical and retrospective clinical evidence shows that androgen receptor (AR)-mediated signals have significant roles in development of non-muscle invasive bladder cancer (NMIBC). Here, we conducted a single-center, phase I study to assess the feasibility and efficacy of enzalutamide in patients having recurrent NMIBC with marker tumors. Patients with NMIBC who cannot achieve complete transurethral resection (TUR) or with recurrence within a year after the TUR, were enrolled. The patients were administered oral enzalutamide at 160 mg dose, once daily for four weeks. Clinical response at the end of the treatment was evaluated using cystoscopy.</p><p><strong>Results: </strong>Of the six patients enrolled, two experienced multiple recurrences. All the patients received the planned administration of enzalutamide. Enzalutamide was tolerable and all patients were able to complete the planed treatment, although four patients experienced mild treatment-related adverse events (AEs), but AEs with grade 2 or more were not observed. As for efficacy, three patients showed no change while the remaining three showed disease progression. Immunohistochemical analysis did not showed the strong staining of AR in the latest tumors. This is the first clinical study on enzalutamide treatment for NMIBC patients. In this study, four weeks of enzalutamide administration was well tolerated, however showed no clinical response for non-strong staining of AR.</p><p><strong>Trial registration: </strong>University Hospital Medical Information Network UMIN000026520 (date registration: 2017/3/13).</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"47"},"PeriodicalIF":1.6,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11786325/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of the chloroplast and mitochondrial genomes of the Indian pitcher plant, Nepenthes khasiana Hook.f.","authors":"Ruchishree Konhar, Debasis Dash, Devendra Kumar Biswal","doi":"10.1186/s13104-025-07082-w","DOIUrl":"10.1186/s13104-025-07082-w","url":null,"abstract":"<p><strong>Objectives: </strong>Nepenthes, sometimes known as tropical pitcher plants or monkey cups, is a carnivorous plant genus that contains more than 160 species. Nepenthes khasiana, India's sole representative of the genus, is a rare and endangered dioecious plant endemic to North-east India. Despite the fact that it is a prominent insectivorous plant in the Nepenthaceae family, genomic resources for the species are limited, making genomic breeding and understanding the genetic basis of botanical carnivory difficult. Herein, we report the complete chloroplast (cp) and mitochondrial (mt) genomes of N. khasiana for the first time. These organelle genomes were assembled as part of a whole-genome sequencing project aimed at gaining deeper insights into their evolutionary relations with genomes of other carnivorous plants.</p><p><strong>Data description: </strong>The complete cp genome (156,914 bp) and mt genome (900,031 bp) of N. khasiana are presented here. The cp genome contains two repeat regions and 131 genes (112 unique genes): 86 protein coding genes, 8 rRNA coding genes and 37 tRNA coding genes. The mt genome contains 84 genes (55 unique genes): 50 protein coding genes, 7 rRNA coding genes and 27 tRNA coding genes. The cp and mt genomic data generated will be useful for future molecular characterization and evolutionary research related to botanical carnivory.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"45"},"PeriodicalIF":1.6,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783808/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of smartphone-based active distraction in association with audioanalgesia for overcoming airotor-related anxiety in children: a randomized controlled trial.","authors":"Kritika Bali, Radhika Ailawadi, Y M Karuna, N Srikant, Ashwin Rao, P Anupama Nayak, Charisma Thimmaiah","doi":"10.1186/s13104-025-07119-0","DOIUrl":"10.1186/s13104-025-07119-0","url":null,"abstract":"<p><strong>Background: </strong>Most children experience distress while visiting a dentist, above which the sound of the airotor and suction machine results in fear and difficulty in performing further procedures.</p><p><strong>Methods: </strong>This was a randomized controlled parallel-group study of 40 children aged 6-13 years who required cavity preparation via the airotor. The children were randomly allocated to either Group 1 (Piano music app; active distraction combined with audio analgesia) or Group 2 (basic behavioural guidance alone). Self-reported dental anxiety was measured via a modified child dental anxiety scale, and behavior was assessed via Venham's and FLACC (Faces Legs Activity Cry and Consolability) scales. The data obtained were subjected to appropriate statistical analysis.</p><p><strong>Results: </strong>Self-reported dental anxiety was significantly lower in group 1 (p < 0.005). No significant difference between the groups was observed for the Venham and FLACC scores.</p><p><strong>Conclusion: </strong>Compared with basic behavioural guidance alone, the use of active distraction with audio analgesia in the form of the piano music app significantly decreased the degree of dental anxiety caused by the use of the airotor. This also resulted in clinically better cooperation by the child during cavity preparation.</p><p><strong>Trial registration: </strong>Registered in the Clinical Trials Registry India (CTRI/2024/07/070160) dated 08/07/2024.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"46"},"PeriodicalIF":1.6,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783720/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}