BMC Research Notes最新文献_第3页

MSRP-TODNet: a multi-scale reinforced region wise analyser for tiny object detection. 用于微小目标检测的多尺度增强区域明智分析仪。

IF 1.6

BMC Research Notes Pub Date : 2025-04-30 DOI: 10.1186/s13104-025-07263-7

Thulasi Bikku, K P N V Satya Sree, Srinivasarao Thota, Malligunta Kiran Kumar, P Shanmugasundaram

{"title":"MSRP-TODNet: a multi-scale reinforced region wise analyser for tiny object detection.","authors":"Thulasi Bikku, K P N V Satya Sree, Srinivasarao Thota, Malligunta Kiran Kumar, P Shanmugasundaram","doi":"10.1186/s13104-025-07263-7","DOIUrl":"https://doi.org/10.1186/s13104-025-07263-7","url":null,"abstract":"Objective: Detecting small, faraway objects in real-time surveillance is challenging due to limited pixel representation, affecting classifier performance. Deep Learning (DL) techniques generate feature maps to enhance detection, but conventional methods suffer from high computational costs. To address this, we propose Multi-Scale Region-wise Pixel Analysis with GAN for Tiny Object Detection (MSRP-TODNet). The model is trained and tested on VisDrone VID 2019 and MS-COCO datasets. First, images undergo two-fold pre-processing using Improved Wiener Filter (IWF) for artifact removal and Adjusted Contrast Enhancement Method (ACEM) for blurring correction. The Multi-Agent Reinforcement Learning (MARL) algorithm splits the pre-processed image into four regions, analyzing each pixel to generate feature maps. These are processed by the Enhanced Feature Pyramid Network (EFPN), which merges them into a single feature map. Finally, a Generative Adversarial Network (GAN) detects objects with bounding boxes.Results: Experimental results on the DOTA dataset demonstrate that MSRP-TODNet outperforms existing state-of-the-art methods. Specifically, it achieves an mAP @0.5 of 84.2%, mAP @0.5:0.95 of 54.1%, and an F1-Score of 84.0%, surpassing improved TPH-YOLOv5, YOLOv7-Tiny, and DRDet by margins of 1.7%-6.1% in detection performance. These results demonstrate the framework's effectiveness for accurate, real-time small object detection in UAV surveillance and aerial imagery.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"200"},"PeriodicalIF":1.6,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044837/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143964918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling within-host Chikungunya virus dynamics with the immune system using semi-analytical approaches. 利用半分析方法模拟宿主内基孔肯雅病毒与免疫系统的动力学。

IF 1.6

BMC Research Notes Pub Date : 2025-04-30 DOI: 10.1186/s13104-025-07252-w

Morufu Oyedunsi Olayiwola, Akeem Olarewaju Yunus, Adedapo Ismaila, Alaje, Joseph Adeleke Adedeji

{"title":"Modeling within-host Chikungunya virus dynamics with the immune system using semi-analytical approaches.","authors":"Morufu Oyedunsi Olayiwola, Akeem Olarewaju Yunus, Adedapo Ismaila, Alaje, Joseph Adeleke Adedeji","doi":"10.1186/s13104-025-07252-w","DOIUrl":"https://doi.org/10.1186/s13104-025-07252-w","url":null,"abstract":"Objective: Chikungunya fever continues to spread worldwide due to its asymptomatic nature and lack of a specific treatment. A mathematical model using the Caputo fractional order derivative is developed to study the interactions between host defense cells and Chikungunya viral particles in this research. The model's solution existence, uniqueness, and positivity are analyzed. The disease-free state threshold and Hyers-Ulam stability are established.Results: The basic reproductive number <math> <mrow><msub><mi>R</mi> <mn>0</mn></msub> <mo>≈</mo> <mn>7</mn></mrow> </math> , depict a high replication rate of the virus, indicating an increased infectiousness of uninfected cells. Sensitivity analysis shows that the invasion rate of susceptible monocytes increases spread, while antigenic immune response keeps <math><msub><mi>R</mi> <mn>0</mn></msub> </math> below 1. The Laplace Adomian Decomposition Method (LADM) is used to solve the model. Experimental outcomes suggest that the enhanced adaptive immune response, potentially influenced by nutritional support or medication, exhibits a more pronounced hysteresis effect. We observed that viral particles are cleared approximately three (3) days earlier before cell infection, potentially clearing the virus within a week. This insight could accelerate elimination of viral particles and expedite virus clearance.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"201"},"PeriodicalIF":1.6,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044791/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143966337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of confidence/trust in the emergency department. 信心/信任在急诊科的作用。

IF 1.6

BMC Research Notes Pub Date : 2025-04-29 DOI: 10.1186/s13104-025-07266-4

Alina Abidova, Pedro Alcântara da Silva, Sérgio Moreira

引用次数: 0

Correction: Simultaneous assessment of mitochondrial DNA copy number and nuclear epigenetic age towards predictive models of development and aging. 更正：同时评估线粒体DNA拷贝数和核表观遗传年龄对发育和衰老的预测模型。

IF 1.6

BMC Research Notes Pub Date : 2025-04-27 DOI: 10.1186/s13104-025-07260-w

Phyo W Win, Julia Nguyen, Amanda L Morin, Charles E Newcomb, Shiva M Singh, Noha Gomaa, Christina A Castellani

引用次数: 0

Sex-specific characteristics of special endurance and performance potential in female runners. 女性跑步者特殊耐力和表现潜力的性别特征。

IF 1.6

BMC Research Notes Pub Date : 2025-04-26 DOI: 10.1186/s13104-025-07256-6

Wolfgang Blödorn, Frank Döring

{"title":"Sex-specific characteristics of special endurance and performance potential in female runners.","authors":"Wolfgang Blödorn, Frank Döring","doi":"10.1186/s13104-025-07256-6","DOIUrl":"https://doi.org/10.1186/s13104-025-07256-6","url":null,"abstract":"Objective: The coefficient of special endurance (KsA) is a metric that quantifies the relative pace loss between two consecutive distances (e.g., 100 m/200m). Here, we analyzed over 20,000 race times to determine KsA values for female runners across seven distance pairs from 100 m to 10,000 m. The data analyses are based on multiple official performance rankings at international to regional levels, exclusively compiled and processed for this study.Results: The KsA values obtained have remained stable for over four decades in national-level female runners and are applicable from world-class to regional levels. A sex-based analysis reveals that females undergo a more pronounced decrease in pace from 100 m to 1500 m in comparison to males. These sex differences in special endurance align with known variations in muscle fiber composition and fast-twitch type II fiber characteristics between males and females. In conclusion, we provide statistically valid KsA reference values for female runners from 100 m to 10,000 m. These values have practical implications for coaches and athletes seeking to assess runners' strengths, weaknesses, potential, and specific talents based on race times. Sex differences in KsA values may reflect muscle physiology and guide future research on KsA and muscle function.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"196"},"PeriodicalIF":1.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12032771/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143980845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The influence of the geroprotective cytokine on the transcriptome of young and senescent mesenchymal stem cells. 衰老保护细胞因子对年轻和衰老间充质干细胞转录组的影响。

IF 1.6

BMC Research Notes Pub Date : 2025-04-24 DOI: 10.1186/s13104-025-07262-8

Liya G Kondratyeva, Diana K Matveeva, Maria I Ezdakova, Marina V Utkina, Andrey Yu Ratushnyy

{"title":"The influence of the geroprotective cytokine on the transcriptome of young and senescent mesenchymal stem cells.","authors":"Liya G Kondratyeva, Diana K Matveeva, Maria I Ezdakova, Marina V Utkina, Andrey Yu Ratushnyy","doi":"10.1186/s13104-025-07262-8","DOIUrl":"https://doi.org/10.1186/s13104-025-07262-8","url":null,"abstract":"Objectives: Increasing longevity and the growing elderly population necessitate a deeper understanding of aging mechanisms to prolong productive life and improve treatments for age-related diseases linked with cellular senescence. Mesenchymal stem cells (MSCs) are crucial for maintaining tissue homeostasis, but their physiological changes during senescence are not well understood. Growth differentiation factor 11 (GDF11) has emerged as a potential rejuvenation factor, enhancing MSC viability, mobility, and angiogenic functions, which improves outcomes in ischemic models and cardiac repair. This study aims to identify transcriptomic changes in young and senescent MSCs influenced by GDF11, highlighting its potential in MSC-based therapies.Data description: To evaluate transcriptomic changes induced by the potential geroprotective factor GDF11, we performed RNA sequencing on four groups of samples: 'young' MSCs (MmC-/GDF11-) and senescent MSCs (MmC+/GDF11-) without the addition of GDF11, as well as 'young' (MmC-/GDF11+) and senescent MSCs (MmC+/GDF11+) with the addition of GDF11. After 10 days of incubation, indexed cDNA libraries for Illumina sequencing were prepared from the samples, and the resulting cDNA library mix was subjected to NovaSeq 6000 sequencing. This paper describes the collection of 16 RNA sequencing samples comprising 4 sets of MSCs. FASTQ files from Illumina sequencing are available in the NCBI Gene Expression Omnibus.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"195"},"PeriodicalIF":1.6,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12023522/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of methods of optimal cut-point selection for biomarkers in diagnostic medicine: a simulation study with application of clinical data in health informatics. 诊断医学中生物标志物最佳切入点选择方法的比较：临床数据在健康信息学中的应用模拟研究。

IF 1.6

BMC Research Notes Pub Date : 2025-04-23 DOI: 10.1186/s13104-025-07245-9

Mojtaba Hassanzad, Karimollah Hajian-Tilaki, Zinatossadat Bouzari, Shahla Yazdani

{"title":"Comparison of methods of optimal cut-point selection for biomarkers in diagnostic medicine: a simulation study with application of clinical data in health informatics.","authors":"Mojtaba Hassanzad, Karimollah Hajian-Tilaki, Zinatossadat Bouzari, Shahla Yazdani","doi":"10.1186/s13104-025-07245-9","DOIUrl":"https://doi.org/10.1186/s13104-025-07245-9","url":null,"abstract":"Objectives: Several methods of cut-point selection for biomarkers have been suggested in biomedical research but the superiority of them over others was not studied comprehensively under different pairs of distributions, degree of overlap, and the ratio of sample sizes. This simulation study was aimed to compare five popular methods with application of clinical examples.Results: The data of simulation was generated from the 12 configurations of binormal, bigamma, and biexponential pairs with different sample sizes The results showed that the four popular methods of Youden, Euclidean, Product, and Index of Union (IU) yielded identical optimal cut-point under binormal model with homoscedastic. While, with high AUC, the Youden may produce less bias and MSE, but for moderate and low AUC, Euclidean has less bias and MSE than other methods. The IU yielded more precise findings than the Youden for moderate and low AUC in binormal pairs, but its performance was lower with skewed distributions. In contrast, the cut-points produced by diagnostic odds ratio (DOR) were extremely high with low sensitivity and high MSE and bias. The results of clinical data showed that when AUC > 0.95, the five methods may produce identical cut-point, but DOR yields an extremely high value of cut-point for AUC < 0.95.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"193"},"PeriodicalIF":1.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12020263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143981307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermal potentiation improves IFN-γ production but not cytotoxicity in human CAR-T cells. 热增强可改善人CAR-T细胞中IFN-γ的产生，但不能改善细胞毒性。

IF 1.6

BMC Research Notes Pub Date : 2025-04-23 DOI: 10.1186/s13104-025-07249-5

Niladri Bhusan Pati, Yixin Jin, Suman Kumar, Jon Amund Kyte, Rafal Ciosk

{"title":"Thermal potentiation improves IFN-γ production but not cytotoxicity in human CAR-T cells.","authors":"Niladri Bhusan Pati, Yixin Jin, Suman Kumar, Jon Amund Kyte, Rafal Ciosk","doi":"10.1186/s13104-025-07249-5","DOIUrl":"https://doi.org/10.1186/s13104-025-07249-5","url":null,"abstract":"Objective: Body temperature plays an important role in cancer, with febrile temperature generally associated with improved cancer resistance. In murine models, this resistance has been linked to the cytotoxic T cells, whose differentiation into cancer-killing effector cells is poor at lower but robust at elevated temperatures. If conserved, temperature-mediated potentiation of patient-derived T cells could be implemented to improve the existing cancer treatments, including the chimeric antigen receptor T-cell therapy (CAR T-cell therapy). Here, we tested this possibility using human STEAP1 CAR-T cells developed to target prostate cancer.Results: In mice, transient exposure to febrile temperature (39-40 ºC) increases the production of IFN-γ and the cancer-killing ability of CD8 + T cells. Using a similar temperature treatment, we observed elevated levels of IFN-γ also in the human CAR-T cells. However, these cells displayed no improvement in their ability to kill cancer cells. Although we cannot discount the possibility that alternative protocols might lead to other outcomes, our findings suggest that incorporating thermal potentiation into existing protocols of CAR-T cell therapy may be more complicated than anticipated.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"192"},"PeriodicalIF":1.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12020131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143976823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using LDpred2 to adapt polygenic risk score techniques for methylation score creation. 使用LDpred2适应多基因风险评分技术创建甲基化评分。

IF 1.6

BMC Research Notes Pub Date : 2025-04-23 DOI: 10.1186/s13104-025-07222-2

Kristoffer Sandås, Leticia Spindola, Solveig Løkhammer, Anne-Kristin Stavrum, Ole Andreassen, Markos Tesfaye, Stéphanie Le Hellard

{"title":"Using LDpred2 to adapt polygenic risk score techniques for methylation score creation.","authors":"Kristoffer Sandås, Leticia Spindola, Solveig Løkhammer, Anne-Kristin Stavrum, Ole Andreassen, Markos Tesfaye, Stéphanie Le Hellard","doi":"10.1186/s13104-025-07222-2","DOIUrl":"https://doi.org/10.1186/s13104-025-07222-2","url":null,"abstract":"Objective: This study sought to determine if the R package LDpred2, designed for polygenic risk score creation for genome-wide association studies using summary statistics, could be adapted for deriving DNA methylation scores from methylome-wide association studies. Recognizing that linkage disequilibrium, used as prior in LDpred2, does not apply to methylation, we explored co-methylated regions and topologically associating domains as alternative structural priors for correlation between methylation sites. A genomic sliding-window approach was also tested. The performance of the LDpred2-based models was evaluated on methylation data from schizophrenia and control samples (N = 1,227).Results: LDpred2 models employing topologically associating domains and sliding window clusters as priors performed similarly to existing methods, explaining approximately 3.6% of schizophrenia phenotypic variance. The co-methylated regions model underperformed due to insufficient clustering of probes. The similarity in performance between the model using topologically associating domains and a null model consisting of random clusters suggests that the structural information provided by these domains enhances performance only marginally. In conclusion, while LDpred2 can be adapted for methylation data, it does not substantially enhance methylation score performance over existing methods, and the choice of structural prior may not be a critical factor.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"190"},"PeriodicalIF":1.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12016360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143989022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive single-cell RNA-sequencing study of Tollip deficiency effect in IL-13-stimulated human airway epithelial cells. il -13刺激的人气道上皮细胞Tollip缺陷效应的单细胞rna测序研究。

IF 1.6

BMC Research Notes Pub Date : 2025-04-23 DOI: 10.1186/s13104-025-07255-7

Grace Yihua Lee, Niccolette Schaunaman, Hamid Reza Nouri, Monica Kraft, Hong Wei Chu

{"title":"Comprehensive single-cell RNA-sequencing study of Tollip deficiency effect in IL-13-stimulated human airway epithelial cells.","authors":"Grace Yihua Lee, Niccolette Schaunaman, Hamid Reza Nouri, Monica Kraft, Hong Wei Chu","doi":"10.1186/s13104-025-07255-7","DOIUrl":"https://doi.org/10.1186/s13104-025-07255-7","url":null,"abstract":"Objective: Toll-interacting protein (Tollip) suppresses excessive pro-inflammatory signaling, but its function in airway epithelial responses to IL-13, a key mediator in allergic diseases, remains unclear. This study investigates Tollip knockdown (TKD) effects in primary human airway epithelial cells using single-cell RNA sequencing, providing the first single-cell analysis of TKD and the first exploring its interaction with IL-13.Results: IL-13 treatment upregulated key genes, including SPDEF, MUC5AC, POSTN, ALOX15, and CCL26, confirming IL-13's effects and validating our methods. IL-13 reduced TNF-α signaling and epithelial-mesenchymal transition in certain cell types, suggesting a dual role in promoting type 2 inflammation while suppressing Th1-driven inflammation. Tollip deficiency alone significantly amplified TNF-α signaling and inflammatory pathways in goblet, club, and suprabasal cells. Comparisons between TKDIL13 vs IL13 and TKD vs CTR revealed that IL-13 does not substantially alter Tollip deficiency response in most cell types, reinforcing findings in TKD vs CTR. Tollip deficiency alters the response to IL-13 in a cell-type-specific manner, strongly downregulating TNF-α signaling in goblet cells but only weakly in basal and club cells. Tollip deficiency enhances IL-13's suppression of Th1 inflammatory responses in goblet cells. These novel insights in Tollip-IL-13 interactions offer potential therapeutic targets for asthma and related diseases.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"194"},"PeriodicalIF":1.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12020103/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143980795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0